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The map label for this gene is rfbF [H]

Identifier: 76819082

GI number: 76819082

Start: 2399506

End: 2400312

Strand: Direct

Name: rfbF [H]

Synonym: BURPS1710b_A1961

Alternate gene names: 76819082

Gene position: 2399506-2400312 (Clockwise)

Preceding gene: 76818741

Following gene: 76819797

Centisome position: 75.41

GC content: 61.96

Gene sequence:

>807_bases
GTGGACGATAACTTTCAAGAGGACGCGATCGTGAAGGCTGTGATTCTCGCAGGCGGATTCGGCACGCGATTCACCGAAGA
AACGATTTCCCGGCCGAAACCGATGATCGAGATCGGCGGCAAGCCGATTCTCTGGCACATCCTGAAGCTGTATTCGCACC
ACGGCATCAACGAGTTCATCATCTGCTGCGGATACCGGGGCTACGTCATCAAGGAATATTTCGCGAACTACTTCCTGCAC
ATGTCGGACGTGACGTTCGATGTGCAGAAGAACAGCATGGAAGTTCATTACCGCTATTCGGAACCGTGGCGCGTGACGCT
CGTCGACACCGGCGAGACGACGATGACGGGCGGGCGGATCCGCCGCGTGCGCCAGTACGTCGAAGGCGACGACATGTTCT
GCCTCACGTACGGCGATGGCCTGTGCGACGTCGACCTCGGCCGCCTGATCGCATTTCACCGCGAGCACGGCGGCAAGGCG
ACGCTCACCGCGGTGCATCCGCCGGCGCGCTTCGGCGTGCTCGATATCGGCGCGGACCGGCAGGTCATCGATTTCACCGA
GAAGCCGCGGATCGACGGCGGGATGATCAACGGCGGGTTCTTCGTGCTGTCGCCTTCGGTCATCGATCTGATCGACGGCG
ACGCGACGGTGTGGGAACGCGAGCCGCTCGAGCGGCTCGCGCGGGCCAACGAGCTGAAGGCGTATCCGCACGACGGCTTC
TGGCAATGCATGGATACGTTGCGCGACAAGCTGCTGCTCGACAGCATGTGGGCGACGGGCACCGCGCCGTGGAAGAAATG
GAGCTGA

Upstream 100 bases:

>100_bases
GCTTCGTGCTGGCGGTGACGGCCGTCATCGGCAGGGCGTTCGGCAGGCAGGCGGTGGAGCGTGCGCGGCGAAGCGGCGAC
CTCGCGCGCCTCAGGCATGC

Downstream 100 bases:

>100_bases
CGATGCATGCGCCCGACGCTCGATTCTGGCAGGCGAGACGCGTGCTCGTGACGGGCCACACGGGCTTCAAGGGAAGCTGG
CTGAGCCTGTGGCTGCAGTC

Product: glucose-1-phosphate cytidylyltransferase

Products: NA

Alternate protein names: CDP-glucose pyrophosphorylase [H]

Number of amino acids: Translated: 268; Mature: 268

Protein sequence:

>268_residues
MDDNFQEDAIVKAVILAGGFGTRFTEETISRPKPMIEIGGKPILWHILKLYSHHGINEFIICCGYRGYVIKEYFANYFLH
MSDVTFDVQKNSMEVHYRYSEPWRVTLVDTGETTMTGGRIRRVRQYVEGDDMFCLTYGDGLCDVDLGRLIAFHREHGGKA
TLTAVHPPARFGVLDIGADRQVIDFTEKPRIDGGMINGGFFVLSPSVIDLIDGDATVWEREPLERLARANELKAYPHDGF
WQCMDTLRDKLLLDSMWATGTAPWKKWS

Sequences:

>Translated_268_residues
MDDNFQEDAIVKAVILAGGFGTRFTEETISRPKPMIEIGGKPILWHILKLYSHHGINEFIICCGYRGYVIKEYFANYFLH
MSDVTFDVQKNSMEVHYRYSEPWRVTLVDTGETTMTGGRIRRVRQYVEGDDMFCLTYGDGLCDVDLGRLIAFHREHGGKA
TLTAVHPPARFGVLDIGADRQVIDFTEKPRIDGGMINGGFFVLSPSVIDLIDGDATVWEREPLERLARANELKAYPHDGF
WQCMDTLRDKLLLDSMWATGTAPWKKWS
>Mature_268_residues
MDDNFQEDAIVKAVILAGGFGTRFTEETISRPKPMIEIGGKPILWHILKLYSHHGINEFIICCGYRGYVIKEYFANYFLH
MSDVTFDVQKNSMEVHYRYSEPWRVTLVDTGETTMTGGRIRRVRQYVEGDDMFCLTYGDGLCDVDLGRLIAFHREHGGKA
TLTAVHPPARFGVLDIGADRQVIDFTEKPRIDGGMINGGFFVLSPSVIDLIDGDATVWEREPLERLARANELKAYPHDGF
WQCMDTLRDKLLLDSMWATGTAPWKKWS

Specific function: Involved in the biosynthesis of the tyvelose, a 3,6- dideoxyhexose found in the O-antigen of the surface lipopolysaccharides. It catalyzes the transfer of a CMP moiety from CTP to glucose 1-phosphate. This enzyme can utilize either CTP or UTP as the nucle

COG id: COG1208

COG function: function code MJ; Nucleoside-diphosphate-sugar pyrophosphorylase involved in lipopolysaccharide biosynthesis/translation initiation factor 2B, gamma/epsilon subunits (eIF-2Bgamma/eIF-2Bepsilon)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glucose-1-phosphate cytidylyltransferase family [H]

Homologues:

Organism=Homo sapiens, GI11761621, Length=251, Percent_Identity=29.4820717131474, Blast_Score=107, Evalue=1e-23,
Organism=Homo sapiens, GI11761619, Length=251, Percent_Identity=29.4820717131474, Blast_Score=107, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI133931050, Length=250, Percent_Identity=28, Blast_Score=116, Evalue=1e-26,
Organism=Saccharomyces cerevisiae, GI6320148, Length=248, Percent_Identity=29.4354838709677, Blast_Score=119, Evalue=7e-28,
Organism=Drosophila melanogaster, GI21355443, Length=249, Percent_Identity=28.1124497991968, Blast_Score=104, Evalue=8e-23,
Organism=Drosophila melanogaster, GI24644084, Length=249, Percent_Identity=28.1124497991968, Blast_Score=104, Evalue=8e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013446
- InterPro:   IPR005835 [H]

Pfam domain/function: PF00483 NTP_transferase [H]

EC number: =2.7.7.33 [H]

Molecular weight: Translated: 30549; Mature: 30549

Theoretical pI: Translated: 5.82; Mature: 5.82

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDDNFQEDAIVKAVILAGGFGTRFTEETISRPKPMIEIGGKPILWHILKLYSHHGINEFI
CCCCCCHHHHHHHHEEECCCCCCCCHHHHHCCCCEEEECCCHHHHHHHHHHHHCCCCEEE
ICCGYRGYVIKEYFANYFLHMSDVTFDVQKNSMEVHYRYSEPWRVTLVDTGETTMTGGRI
EEECCCCHHHHHHHHHHEEEECCEEEEEECCCEEEEEEECCCEEEEEEECCCCEECCHHH
RRVRQYVEGDDMFCLTYGDGLCDVDLGRLIAFHREHGGKATLTAVHPPARFGVLDIGADR
HHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHCCCCEEEEEEECCCCCCCEEEECCCC
QVIDFTEKPRIDGGMINGGFFVLSPSVIDLIDGDATVWEREPLERLARANELKAYPHDGF
EEEECCCCCCCCCCEECCCEEEECCCEEEEECCCCCEECHHHHHHHHHHHHCCCCCCCHH
WQCMDTLRDKLLLDSMWATGTAPWKKWS
HHHHHHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MDDNFQEDAIVKAVILAGGFGTRFTEETISRPKPMIEIGGKPILWHILKLYSHHGINEFI
CCCCCCHHHHHHHHEEECCCCCCCCHHHHHCCCCEEEECCCHHHHHHHHHHHHCCCCEEE
ICCGYRGYVIKEYFANYFLHMSDVTFDVQKNSMEVHYRYSEPWRVTLVDTGETTMTGGRI
EEECCCCHHHHHHHHHHEEEECCEEEEEECCCEEEEEEECCCEEEEEEECCCCEECCHHH
RRVRQYVEGDDMFCLTYGDGLCDVDLGRLIAFHREHGGKATLTAVHPPARFGVLDIGADR
HHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHCCCCEEEEEEECCCCCCCEEEECCCC
QVIDFTEKPRIDGGMINGGFFVLSPSVIDLIDGDATVWEREPLERLARANELKAYPHDGF
EEEECCCCCCCCCCEECCCEEEECCCEEEEECCCCCEECHHHHHHHHHHHHCCCCCCCHH
WQCMDTLRDKLLLDSMWATGTAPWKKWS
HHHHHHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11677608; 12644504 [H]