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The map label for this gene is gutB [H]

Identifier: 76818960

GI number: 76818960

Start: 2185265

End: 2186401

Strand: Direct

Name: gutB [H]

Synonym: BURPS1710b_A1795

Alternate gene names: 76818960

Gene position: 2185265-2186401 (Clockwise)

Preceding gene: 76817522

Following gene: 76819479

Centisome position: 68.68

GC content: 68.43

Gene sequence:

>1137_bases
ATGCCACAGACAGCCGAAGCGCCGCCGCAACGCGCGGCCGAGCGCGGCGAGACGCCCGCGCTGCCCGCCACGATGCGCGC
CGTGGTTTGCCACGGCCCGCGAGACTACCGCCTCGAGCAGGTGCCGGTGCCGAAGCCGAGGCCGGACGAGATCCTGACCC
AGGTGGAGCGCGTGGGCATCTGCATGGGCGACATCAAGACGTTTCGCGGCGCGCCGTCGTTCTGGGGCGACGCGGTGCAG
CCGCGCTACGTGAAGCCGCCGATGATTCCCGGCCACGAATTCGTGTGCCGCGTCGTCGCGCTCGGCCCCGGCGCCGAGCG
GCGCGGCGTGAAGGCGGGCGATCGCGTGATCTCCGAGCAGATCGTGCCGTGCTGGAGCTGCCGCTTCTGCGGCCACGGCC
AGTACTGGATGTGCCAGAAGCACGATCTGTACGGATTCCAGAACAACGTGCACGGCGCGATGGCCGAATACATGATCTTC
ACGAAGGAGGCGATCGTGCACCGCGTGCCCGATTCGATCCCGACCGACGAGGCGATCCTGATCGAGCCGCTGTCGTGCTC
GCTGCACGCGGCCGATCGCGCGAACGTCGGCTTCGACGACGTGGTCGTCGTCGCCGGCGCGGGCACGCTCGGGCTCGGCA
TCATCGGCGCGGTGCGGCTGCGCCATCCGAAGCAACTGATCGTGCTCGACATGAAGCCCGAGCGCGCGGCGCTCGCGCGC
CGGATGGGCGCGGACGACGTGTGGAACCCGGCCGAGGAGAACGTGATCGAGAAGATCCGCGCGATCACGGGCGGCTACGG
CTGCGATATCTACATCGAGGCGACCGGCCACCATCGCGCGGTAGGCCAGGGGCTCGCGATGCTGCGCAAGCTCGGGCGCT
TCGTCGAGTTCAGCGTGTTCAACGACGAAGCGAGCGTCGACTGGTCGATCATCGGCGATCGCAAGGAGCTCGACGTGCTC
GGCTCGCATCTCGGCCCGTACATGTACCCGCGCGCGATCGAGTTCATCGCATCGCGCAGGATCGACGTGCGCGGCATCGT
CACGCACACGTTCCCGCTGTCGCGCTTCGCCGACGCGTTCGCGGTGATGGAGCGCGGCGAGCAATCGTTGAAGGTCGTTC
TGGATCCGCGAGGTTAA

Upstream 100 bases:

>100_bases
ACGTCGTCATCGACGGCGGCTACACCGTGCGCTGAAGCGGCGGCCGCGCAATCATCGCCGCCCTCCCCGATCGACCGACA
CACAGCACAGGAGACAACGC

Downstream 100 bases:

>100_bases
TTGCATGAATCGTGTCATCAATCATCCCGACTACGTCGTCGAGGACATGCTGCGCGGGATCGTCGCCGCGCATCCGGCGC
TCGCGCTCGACGCGGACAAC

Product: zinc-binding dehydrogenase family oxidoreductase

Products: NA

Alternate protein names: Glucitol dehydrogenase; L-iditol 2-dehydrogenase [H]

Number of amino acids: Translated: 378; Mature: 377

Protein sequence:

>378_residues
MPQTAEAPPQRAAERGETPALPATMRAVVCHGPRDYRLEQVPVPKPRPDEILTQVERVGICMGDIKTFRGAPSFWGDAVQ
PRYVKPPMIPGHEFVCRVVALGPGAERRGVKAGDRVISEQIVPCWSCRFCGHGQYWMCQKHDLYGFQNNVHGAMAEYMIF
TKEAIVHRVPDSIPTDEAILIEPLSCSLHAADRANVGFDDVVVVAGAGTLGLGIIGAVRLRHPKQLIVLDMKPERAALAR
RMGADDVWNPAEENVIEKIRAITGGYGCDIYIEATGHHRAVGQGLAMLRKLGRFVEFSVFNDEASVDWSIIGDRKELDVL
GSHLGPYMYPRAIEFIASRRIDVRGIVTHTFPLSRFADAFAVMERGEQSLKVVLDPRG

Sequences:

>Translated_378_residues
MPQTAEAPPQRAAERGETPALPATMRAVVCHGPRDYRLEQVPVPKPRPDEILTQVERVGICMGDIKTFRGAPSFWGDAVQ
PRYVKPPMIPGHEFVCRVVALGPGAERRGVKAGDRVISEQIVPCWSCRFCGHGQYWMCQKHDLYGFQNNVHGAMAEYMIF
TKEAIVHRVPDSIPTDEAILIEPLSCSLHAADRANVGFDDVVVVAGAGTLGLGIIGAVRLRHPKQLIVLDMKPERAALAR
RMGADDVWNPAEENVIEKIRAITGGYGCDIYIEATGHHRAVGQGLAMLRKLGRFVEFSVFNDEASVDWSIIGDRKELDVL
GSHLGPYMYPRAIEFIASRRIDVRGIVTHTFPLSRFADAFAVMERGEQSLKVVLDPRG
>Mature_377_residues
PQTAEAPPQRAAERGETPALPATMRAVVCHGPRDYRLEQVPVPKPRPDEILTQVERVGICMGDIKTFRGAPSFWGDAVQP
RYVKPPMIPGHEFVCRVVALGPGAERRGVKAGDRVISEQIVPCWSCRFCGHGQYWMCQKHDLYGFQNNVHGAMAEYMIFT
KEAIVHRVPDSIPTDEAILIEPLSCSLHAADRANVGFDDVVVVAGAGTLGLGIIGAVRLRHPKQLIVLDMKPERAALARR
MGADDVWNPAEENVIEKIRAITGGYGCDIYIEATGHHRAVGQGLAMLRKLGRFVEFSVFNDEASVDWSIIGDRKELDVLG
SHLGPYMYPRAIEFIASRRIDVRGIVTHTFPLSRFADAFAVMERGEQSLKVVLDPRG

Specific function: Reduces glucitol to fructose [H]

COG id: COG1063

COG function: function code ER; Threonine dehydrogenase and related Zn-dependent dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the zinc-containing alcohol dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI156627571, Length=351, Percent_Identity=30.1994301994302, Blast_Score=154, Evalue=1e-37,
Organism=Homo sapiens, GI4501929, Length=372, Percent_Identity=22.3118279569892, Blast_Score=79, Evalue=5e-15,
Organism=Homo sapiens, GI34577061, Length=381, Percent_Identity=22.8346456692913, Blast_Score=70, Evalue=4e-12,
Organism=Escherichia coli, GI1788073, Length=328, Percent_Identity=30.7926829268293, Blast_Score=159, Evalue=2e-40,
Organism=Escherichia coli, GI1790045, Length=339, Percent_Identity=28.6135693215339, Blast_Score=125, Evalue=3e-30,
Organism=Escherichia coli, GI1787863, Length=357, Percent_Identity=26.3305322128852, Blast_Score=117, Evalue=1e-27,
Organism=Escherichia coli, GI226510992, Length=339, Percent_Identity=27.1386430678466, Blast_Score=116, Evalue=3e-27,
Organism=Escherichia coli, GI1788407, Length=363, Percent_Identity=24.5179063360882, Blast_Score=108, Evalue=5e-25,
Organism=Escherichia coli, GI1788075, Length=367, Percent_Identity=25.8855585831063, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI87082125, Length=369, Percent_Identity=27.10027100271, Blast_Score=99, Evalue=4e-22,
Organism=Escherichia coli, GI1786825, Length=411, Percent_Identity=26.2773722627737, Blast_Score=93, Evalue=3e-20,
Organism=Escherichia coli, GI1790718, Length=330, Percent_Identity=24.8484848484848, Blast_Score=93, Evalue=3e-20,
Organism=Escherichia coli, GI1786552, Length=384, Percent_Identity=25.5208333333333, Blast_Score=78, Evalue=9e-16,
Organism=Caenorhabditis elegans, GI17562876, Length=345, Percent_Identity=29.8550724637681, Blast_Score=155, Evalue=4e-38,
Organism=Caenorhabditis elegans, GI17562878, Length=344, Percent_Identity=28.1976744186047, Blast_Score=139, Evalue=2e-33,
Organism=Caenorhabditis elegans, GI71988145, Length=365, Percent_Identity=24.3835616438356, Blast_Score=71, Evalue=8e-13,
Organism=Caenorhabditis elegans, GI17562584, Length=379, Percent_Identity=24.5382585751979, Blast_Score=71, Evalue=8e-13,
Organism=Caenorhabditis elegans, GI17562582, Length=146, Percent_Identity=34.2465753424658, Blast_Score=68, Evalue=7e-12,
Organism=Saccharomyces cerevisiae, GI6319955, Length=362, Percent_Identity=25.1381215469613, Blast_Score=110, Evalue=4e-25,
Organism=Saccharomyces cerevisiae, GI6322619, Length=362, Percent_Identity=25.1381215469613, Blast_Score=110, Evalue=4e-25,
Organism=Saccharomyces cerevisiae, GI6323099, Length=344, Percent_Identity=27.3255813953488, Blast_Score=108, Evalue=1e-24,
Organism=Saccharomyces cerevisiae, GI6319257, Length=387, Percent_Identity=25.5813953488372, Blast_Score=98, Evalue=2e-21,
Organism=Saccharomyces cerevisiae, GI6320033, Length=298, Percent_Identity=25.503355704698, Blast_Score=87, Evalue=4e-18,
Organism=Saccharomyces cerevisiae, GI6319258, Length=299, Percent_Identity=25.752508361204, Blast_Score=84, Evalue=4e-17,
Organism=Saccharomyces cerevisiae, GI6323729, Length=374, Percent_Identity=23.5294117647059, Blast_Score=77, Evalue=4e-15,
Organism=Saccharomyces cerevisiae, GI6323961, Length=381, Percent_Identity=24.9343832020997, Blast_Score=75, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6319621, Length=373, Percent_Identity=27.8820375335121, Blast_Score=75, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6324486, Length=382, Percent_Identity=25.6544502617801, Blast_Score=72, Evalue=2e-13,
Organism=Drosophila melanogaster, GI17737897, Length=343, Percent_Identity=30.0291545189504, Blast_Score=127, Evalue=1e-29,
Organism=Drosophila melanogaster, GI17137530, Length=342, Percent_Identity=28.9473684210526, Blast_Score=125, Evalue=6e-29,
Organism=Drosophila melanogaster, GI17737895, Length=378, Percent_Identity=23.2804232804233, Blast_Score=80, Evalue=2e-15,
Organism=Drosophila melanogaster, GI221457811, Length=335, Percent_Identity=25.0746268656716, Blast_Score=67, Evalue=3e-11,
Organism=Drosophila melanogaster, GI45550770, Length=335, Percent_Identity=25.0746268656716, Blast_Score=67, Evalue=3e-11,
Organism=Drosophila melanogaster, GI45551930, Length=335, Percent_Identity=25.0746268656716, Blast_Score=67, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013149
- InterPro:   IPR013154
- InterPro:   IPR002085
- InterPro:   IPR002328
- InterPro:   IPR011032
- InterPro:   IPR016040 [H]

Pfam domain/function: PF08240 ADH_N; PF00107 ADH_zinc_N [H]

EC number: =1.1.1.14 [H]

Molecular weight: Translated: 41767; Mature: 41636

Theoretical pI: Translated: 7.31; Mature: 7.31

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPQTAEAPPQRAAERGETPALPATMRAVVCHGPRDYRLEQVPVPKPRPDEILTQVERVGI
CCCCCCCCHHHHHHCCCCCCCCHHHHEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHH
CMGDIKTFRGAPSFWGDAVQPRYVKPPMIPGHEFVCRVVALGPGAERRGVKAGDRVISEQ
HHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHEEEEEECCCCCCCCCCHHHHHHHHH
IVPCWSCRFCGHGQYWMCQKHDLYGFQNNVHGAMAEYMIFTKEAIVHRVPDSIPTDEAIL
CCCCEEEEECCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEEE
IEPLSCSLHAADRANVGFDDVVVVAGAGTLGLGIIGAVRLRHPKQLIVLDMKPERAALAR
EECCCCEEECCCCCCCCCCCEEEEECCCHHHHHHHHHHHCCCCCEEEEEECCCHHHHHHH
RMGADDVWNPAEENVIEKIRAITGGYGCDIYIEATGHHRAVGQGLAMLRKLGRFVEFSVF
HCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHEEEEEE
NDEASVDWSIIGDRKELDVLGSHLGPYMYPRAIEFIASRRIDVRGIVTHTFPLSRFADAF
CCCCCCEEEEECCCHHHHHHHHHCCCHHHHHHHHHHHHCCCCEEEEEEECCCHHHHHHHH
AVMERGEQSLKVVLDPRG
HHHHCCCCEEEEEECCCC
>Mature Secondary Structure 
PQTAEAPPQRAAERGETPALPATMRAVVCHGPRDYRLEQVPVPKPRPDEILTQVERVGI
CCCCCCCHHHHHHCCCCCCCCHHHHEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHH
CMGDIKTFRGAPSFWGDAVQPRYVKPPMIPGHEFVCRVVALGPGAERRGVKAGDRVISEQ
HHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHEEEEEECCCCCCCCCCHHHHHHHHH
IVPCWSCRFCGHGQYWMCQKHDLYGFQNNVHGAMAEYMIFTKEAIVHRVPDSIPTDEAIL
CCCCEEEEECCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEEE
IEPLSCSLHAADRANVGFDDVVVVAGAGTLGLGIIGAVRLRHPKQLIVLDMKPERAALAR
EECCCCEEECCCCCCCCCCCEEEEECCCHHHHHHHHHHHCCCCCEEEEEECCCHHHHHHH
RMGADDVWNPAEENVIEKIRAITGGYGCDIYIEATGHHRAVGQGLAMLRKLGRFVEFSVF
HCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHEEEEEE
NDEASVDWSIIGDRKELDVLGSHLGPYMYPRAIEFIASRRIDVRGIVTHTFPLSRFADAF
CCCCCCEEEEECCCHHHHHHHHHCCCHHHHHHHHHHHHCCCCEEEEEEECCCHHHHHHHH
AVMERGEQSLKVVLDPRG
HHHHCCCCEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1460002; 9384377; 8195086 [H]