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The map label for this gene is pinE [H]

Identifier: 76818748

GI number: 76818748

Start: 1494243

End: 1494839

Strand: Direct

Name: pinE [H]

Synonym: BURPS1710b_A1190

Alternate gene names: 76818748

Gene position: 1494243-1494839 (Clockwise)

Preceding gene: 76817438

Following gene: 76818860

Centisome position: 46.96

GC content: 62.14

Gene sequence:

>597_bases
ATGACCAGCACCTCGATCTGCAACGCGACGCGCTGGCGAAGGCAGGCTGTGAACGCGTCTTCGAGGACACCGCAAGCGGA
GCGCACCGGCTTGACGGCGCTGCTTGCCAGCTTGCGTCAAGGTGACACGATAGTAATCTGGCGGCTGGACCGACTCGGGC
GGTCACTGAAAGACCTGATCTACCTGGTCGAGCGGTTCGAAGCCGCCGGCGTCGGCCTGCGCAGCTTGCAGGAGAGCGTT
GACACCGCGTCTATCGGCGGCCGCCTCGTGTTTCATTTGTTCGGCGCATTGGCAGAGTTCGAGCGCAACCTGATCCGCGC
TGAAAGACTGCCACTCGGTTCGAGCCGATCCGCAAAATCGGTGAACATGCTGTGCCATTTCCGGACCATCCCATGGCTCG
TGCCGACAAGGTCCGCCACCTGCATGACCAACAACGGCAGCGACAGGCGCCGAATGACCAGGCGCATGCGCTCGGGCGAA
CTCATCTTCGCATTGACGAACGGCGTGCCGCGGCGTCGGGTGAATTTGGTCCCGCAATTCGCGCATTTGAACCGTGGGAA
GGCGCCGTTCGAGCATTCATCGAAACGCGTCTGGTGA

Upstream 100 bases:

>100_bases
ATCCAATAGCGGTATCCATTTTTTAATGTCTGACTATACTGAATTTTCACCGGCCAAACTGGAATCCGTATGCTAATCGG
ATACGCGCGCGTCTCGGCGG

Downstream 100 bases:

>100_bases
CTGCCGCAGTTCGGGCAATGCGGAGGTCGATGGCTGAGCCTCACGCGGCGCTCCCACTTGCCGCTCGGGTCGAGCTCCAA
CAGCCACGCGCGCCAGGCCG

Product: invertase/recombinase protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 198; Mature: 197

Protein sequence:

>198_residues
MTSTSICNATRWRRQAVNASSRTPQAERTGLTALLASLRQGDTIVIWRLDRLGRSLKDLIYLVERFEAAGVGLRSLQESV
DTASIGGRLVFHLFGALAEFERNLIRAERLPLGSSRSAKSVNMLCHFRTIPWLVPTRSATCMTNNGSDRRRMTRRMRSGE
LIFALTNGVPRRRVNLVPQFAHLNRGKAPFEHSSKRVW

Sequences:

>Translated_198_residues
MTSTSICNATRWRRQAVNASSRTPQAERTGLTALLASLRQGDTIVIWRLDRLGRSLKDLIYLVERFEAAGVGLRSLQESV
DTASIGGRLVFHLFGALAEFERNLIRAERLPLGSSRSAKSVNMLCHFRTIPWLVPTRSATCMTNNGSDRRRMTRRMRSGE
LIFALTNGVPRRRVNLVPQFAHLNRGKAPFEHSSKRVW
>Mature_197_residues
TSTSICNATRWRRQAVNASSRTPQAERTGLTALLASLRQGDTIVIWRLDRLGRSLKDLIYLVERFEAAGVGLRSLQESVD
TASIGGRLVFHLFGALAEFERNLIRAERLPLGSSRSAKSVNMLCHFRTIPWLVPTRSATCMTNNGSDRRRMTRRMRSGEL
IFALTNGVPRRRVNLVPQFAHLNRGKAPFEHSSKRVW

Specific function: This protein catalyzes the inversion of an 1800-bp E.coli DNA fragment, the P region, which can exist in either orientation. The function of the inversion is not yet clear [H]

COG id: COG1961

COG function: function code L; Site-specific recombinases, DNA invertase Pin homologs

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the site-specific recombinase resolvase family [H]

Homologues:

Organism=Escherichia coli, GI1787404, Length=81, Percent_Identity=53.0864197530864, Blast_Score=92, Evalue=3e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009057
- InterPro:   IPR012287
- InterPro:   IPR006118
- InterPro:   IPR006119
- InterPro:   IPR006120 [H]

Pfam domain/function: PF02796 HTH_7; PF00239 Resolvase [H]

EC number: NA

Molecular weight: Translated: 22375; Mature: 22244

Theoretical pI: Translated: 12.23; Mature: 12.23

Prosite motif: PS00398 RECOMBINASES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSTSICNATRWRRQAVNASSRTPQAERTGLTALLASLRQGDTIVIWRLDRLGRSLKDLI
CCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCEEEEEEHHHHCHHHHHHH
YLVERFEAAGVGLRSLQESVDTASIGGRLVFHLFGALAEFERNLIRAERLPLGSSRSAKS
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHH
VNMLCHFRTIPWLVPTRSATCMTNNGSDRRRMTRRMRSGELIFALTNGVPRRRVNLVPQF
HHHHHEECCCCEEECCCCCEEEECCCHHHHHHHHHHCCCCEEEEECCCCCCHHHHCCHHH
AHLNRGKAPFEHSSKRVW
HHCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
TSTSICNATRWRRQAVNASSRTPQAERTGLTALLASLRQGDTIVIWRLDRLGRSLKDLI
CCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCEEEEEEHHHHCHHHHHHH
YLVERFEAAGVGLRSLQESVDTASIGGRLVFHLFGALAEFERNLIRAERLPLGSSRSAKS
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHH
VNMLCHFRTIPWLVPTRSATCMTNNGSDRRRMTRRMRSGELIFALTNGVPRRRVNLVPQF
HHHHHEECCCCEEECCCCCEEEECCCHHHHHHHHHHCCCCEEEEECCCCCCHHHHCCHHH
AHLNRGKAPFEHSSKRVW
HHCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3894006; 6310572; 8905232; 9278503 [H]