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The map label for this gene is benB [H]

Identifier: 76818735

GI number: 76818735

Start: 1264168

End: 1266261

Strand: Direct

Name: benB [H]

Synonym: BURPS1710b_A1000

Alternate gene names: 76818735

Gene position: 1264168-1266261 (Clockwise)

Preceding gene: 76818281

Following gene: 76818275

Centisome position: 39.73

GC content: 65.38

Gene sequence:

>2094_bases
ATGCCGCAGTTCCATCTTCATACCTCGAGGATATCGATCCGATACTCAATCAGTGTTGGACGCACCGTTTTGCCGGCAAC
TATCCTCAATCTGACCGCGCTGTCGTCGAGCACTATACCTGCGAAGCATCGCAGGTTCACCCGGGGTTTCCCATGCGACG
CGCGGCGCGGCGCCTCGCCGGACCGCGCGTCCGCACGCGCGCCGCCCCCGATCAGAGAGCCCATCAGGAGACGCCCCCAT
GATCCCGATCGACCCGGACCGCCGTGCGACGCCGCGCCCCATCGACGATTTCCTCGTCGAAGACAAGGCGCGCGGCGACT
ACCGGCTGCACCGCAGCGCGTTCACCGACGAAATGCTGTTCGAGCTCGAGATGAAGCACATCTTCGAAGGCAACTGGATC
TATCTCGCGCACGAGAGCCAGCTCCCGAACGCGAACGATTACTACACGACCACGATCGGCCGCCAGCCGATCGTGATCGC
GCGCAACCGCCACGGCGAGCTGAACGCGTTCGTCAACGCCTGCACGCACCGCGGCGCGATGCTGTGCCGCCACAAGCGCG
GCAACCGCGCGAGCTACACGTGCCCGTTCCACGGCTGGACGTTCAGCAACGGCGGCAAGCTGCTCAAAGTGAAGGACCCC
GAAGGAGCCGGCTACCCGGACTGCTTCAACCGCGACGGCTCGCACGATCTGAAGAAAGTCGCGCGCTTCGAGAACTATCG
CGGCTTCCTGTTCGGCAGCCTGAACCCCGAAGTCGAGCCGCTCGCCGCGCATCTCGGCGATGCCGCGCGCATCATCGACA
TGATCGTCGATCAGTCGGCGGACGGCCTCGAGGTGCTGCGCGGCTCGTCGACGTACACGTACGAAGGCAACTGGAAGCTC
ACCGCCGAGAACGGCGCGGACGGCTACCACGTATCGGCCGTTCACTGGAACTACGCGGCGACCGTCAACCACCGCAAGAC
CGACGCGCAGCACGAAGACACGATCCGCGCGATGGACGCGGGCAACTGGGGCCGTCAGGGCGGCGGCTTCTACGCGTTCG
ATCACGGCCACATGCTGCTGTGGACGCGCTGGGCGAACCCGGAGGACCGGCCGAACTTCGATCGCCGCGACGAATTCGCC
GCGCGCTGCGGCGGCGACGTCGCCGACTGGATGATCCGGAACTCCCGCAACCTGTGCCTGTACCCGAACGTCTATCTGAT
GGACCAGTTCGGCTCGCAGATCCGCGTGCTGCGCCCGCTCGCCGTCGATCGCACCGAGGTCACGATCTACTGCATCGCGC
CGAAGGGCGAGGCGCCCGACGCGCGCGCGCGGCGCATCCGCCAGTACGAGGATTTCTTCAACGCGAGCGGAATGGCGACG
CCCGACGATCTCGAGGAATTCCGCGCGTGCCAGCAGGGCTACGCGGGCCGCGCGGTCGAATGGAACGACATGTGCCGCGG
CGCCTCGCACTGGATCGAGGGCCCCGACGAAGCCGCGCGCCGGATCGGCATCCGCCCGCTGATGAGCGGCGTGAAGACCG
AGGACGAAGGGCTGTACACGGTCCAGCACCGCTACTGGATCGCGACGATGAAGCAGGCGCTCGCCGCCGAAAGGAGCGGC
GCATGAAGACGATCGATCTCGCCGACATCCAGGCGTTCCTCTATCGCGAGAGCCGGCTGCTCGACGACAAGGCGTGGGAC
GCATGGCTCGACTGCTATCGCGCGGACGCCGTGTTCTGGATGCCTTCCTGGGACGACGCGGATGCGCTCGTCACCGATCC
GCAGCGCGAAATCTCGCTGATCTACTACCCGAACCGGCAGGGGCTCGAGGATCGCGTGTTCCGCATCAAGACCGAGCGCT
CGAGCGCGACGGTGCCCGATACGCGCACGAGCCACAACATCGCGAACGTCGAGCGCGAAAGCGCCGATGGCGACGTGCAC
ACCGTGCGCTTCAACTGGCACACGCTCAGCTATCGCTACAAGACGGTGTCGAGCTATTTCGGCATGTCGCGCTACGCGAT
CGATTTCTCCGGCGACGCGCCGAAGATCGTCAGCAAGTACGTCGTCCTGAAGAACGACTACATCAACCAGCTGATCGACA
TCTACCACATCTAG

Upstream 100 bases:

>100_bases
GCTGCTGGATCTGGCGGCTCAACGGCGGCTGCGCGATGTGCAGGCGCTCGGCCGCGCGCGTGAAGTTCTGCTCCTCCGCG
ACCGCGACGAAGTAGCGTAG

Downstream 100 bases:

>100_bases
CCGGTGCGCGCCGCGGCAGCGCGGCCCGCTCGAGGAACGCATCATGGAACACCGCATCGCCCTGCAGTTCGAAGACGGCG
TCACCCGCTTCATCGCCTGC

Product: benzoate 1,2-dioxygenase subunit beta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 697; Mature: 696

Protein sequence:

>697_residues
MPQFHLHTSRISIRYSISVGRTVLPATILNLTALSSSTIPAKHRRFTRGFPCDARRGASPDRASARAPPPIREPIRRRPH
DPDRPGPPCDAAPHRRFPRRRQGARRLPAAPQRVHRRNAVRARDEAHLRRQLDLSRAREPAPERERLLHDHDRPPADRDR
AQPPRRAERVRQRLHAPRRDAVPPQARQPRELHVPVPRLDVQQRRQAAQSEGPRRSRLPGLLQPRRLARSEESRALRELS
RLPVRQPEPRSRAARRASRRCRAHHRHDRRSVGGRPRGAARLVDVHVRRQLEAHRRERRGRLPRIGRSLELRGDRQPPQD
RRAARRHDPRDGRGQLGPSGRRLLRVRSRPHAAVDALGEPGGPAELRSPRRIRRALRRRRRRLDDPELPQPVPVPERLSD
GPVRLADPRAAPARRRSHRGHDLLHRAEGRGARRARAAHPPVRGFLQRERNGDARRSRGIPRVPAGLRGPRGRMERHVPR
RLALDRGPRRSRAPDRHPPADERREDRGRRAVHGPAPLLDRDDEAGARRRKERRMKTIDLADIQAFLYRESRLLDDKAWD
AWLDCYRADAVFWMPSWDDADALVTDPQREISLIYYPNRQGLEDRVFRIKTERSSATVPDTRTSHNIANVERESADGDVH
TVRFNWHTLSYRYKTVSSYFGMSRYAIDFSGDAPKIVSKYVVLKNDYINQLIDIYHI

Sequences:

>Translated_697_residues
MPQFHLHTSRISIRYSISVGRTVLPATILNLTALSSSTIPAKHRRFTRGFPCDARRGASPDRASARAPPPIREPIRRRPH
DPDRPGPPCDAAPHRRFPRRRQGARRLPAAPQRVHRRNAVRARDEAHLRRQLDLSRAREPAPERERLLHDHDRPPADRDR
AQPPRRAERVRQRLHAPRRDAVPPQARQPRELHVPVPRLDVQQRRQAAQSEGPRRSRLPGLLQPRRLARSEESRALRELS
RLPVRQPEPRSRAARRASRRCRAHHRHDRRSVGGRPRGAARLVDVHVRRQLEAHRRERRGRLPRIGRSLELRGDRQPPQD
RRAARRHDPRDGRGQLGPSGRRLLRVRSRPHAAVDALGEPGGPAELRSPRRIRRALRRRRRRLDDPELPQPVPVPERLSD
GPVRLADPRAAPARRRSHRGHDLLHRAEGRGARRARAAHPPVRGFLQRERNGDARRSRGIPRVPAGLRGPRGRMERHVPR
RLALDRGPRRSRAPDRHPPADERREDRGRRAVHGPAPLLDRDDEAGARRRKERRMKTIDLADIQAFLYRESRLLDDKAWD
AWLDCYRADAVFWMPSWDDADALVTDPQREISLIYYPNRQGLEDRVFRIKTERSSATVPDTRTSHNIANVERESADGDVH
TVRFNWHTLSYRYKTVSSYFGMSRYAIDFSGDAPKIVSKYVVLKNDYINQLIDIYHI
>Mature_696_residues
PQFHLHTSRISIRYSISVGRTVLPATILNLTALSSSTIPAKHRRFTRGFPCDARRGASPDRASARAPPPIREPIRRRPHD
PDRPGPPCDAAPHRRFPRRRQGARRLPAAPQRVHRRNAVRARDEAHLRRQLDLSRAREPAPERERLLHDHDRPPADRDRA
QPPRRAERVRQRLHAPRRDAVPPQARQPRELHVPVPRLDVQQRRQAAQSEGPRRSRLPGLLQPRRLARSEESRALRELSR
LPVRQPEPRSRAARRASRRCRAHHRHDRRSVGGRPRGAARLVDVHVRRQLEAHRRERRGRLPRIGRSLELRGDRQPPQDR
RAARRHDPRDGRGQLGPSGRRLLRVRSRPHAAVDALGEPGGPAELRSPRRIRRALRRRRRRLDDPELPQPVPVPERLSDG
PVRLADPRAAPARRRSHRGHDLLHRAEGRGARRARAAHPPVRGFLQRERNGDARRSRGIPRVPAGLRGPRGRMERHVPRR
LALDRGPRRSRAPDRHPPADERREDRGRRAVHGPAPLLDRDDEAGARRRKERRMKTIDLADIQAFLYRESRLLDDKAWDA
WLDCYRADAVFWMPSWDDADALVTDPQREISLIYYPNRQGLEDRVFRIKTERSSATVPDTRTSHNIANVERESADGDVHT
VRFNWHTLSYRYKTVSSYFGMSRYAIDFSGDAPKIVSKYVVLKNDYINQLIDIYHI

Specific function: Degradation of benzoate to 2-hydro-1,2-dihydroxybenzoate (DHB). The beta subunit may be responsible for the substrate specificity of the enzyme [H]

COG id: COG5517

COG function: function code Q; Small subunit of phenylpropionate dioxygenase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial ring-hydroxylating dioxygenase beta subunit family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017641
- InterPro:   IPR000391 [H]

Pfam domain/function: PF00866 Ring_hydroxyl_B [H]

EC number: =1.14.12.10 [H]

Molecular weight: Translated: 80871; Mature: 80740

Theoretical pI: Translated: 12.26; Mature: 12.26

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPQFHLHTSRISIRYSISVGRTVLPATILNLTALSSSTIPAKHRRFTRGFPCDARRGASP
CCCCEEEECEEEEEEEECCCCHHHHHHHHHHHHHCCCCCCHHHHHHCCCCCCCCCCCCCC
DRASARAPPPIREPIRRRPHDPDRPGPPCDAAPHRRFPRRRQGARRLPAAPQRVHRRNAV
CCCCCCCCCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHCCCCCHHHHHHHHHH
RARDEAHLRRQLDLSRAREPAPERERLLHDHDRPPADRDRAQPPRRAERVRQRLHAPRRD
HHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHCCCCCC
AVPPQARQPRELHVPVPRLDVQQRRQAAQSEGPRRSRLPGLLQPRRLARSEESRALRELS
CCCCCCCCCCEEECCCCCCCHHHHHHHHHHCCCCHHCCCCCCCHHHHHHHHHHHHHHHHH
RLPVRQPEPRSRAARRASRRCRAHHRHDRRSVGGRPRGAARLVDVHVRRQLEAHRRERRG
HCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCC
RLPRIGRSLELRGDRQPPQDRRAARRHDPRDGRGQLGPSGRRLLRVRSRPHAAVDALGEP
CCCCCCCCEEECCCCCCCHHHHHHHHCCCCCCCCCCCCCHHHHHHHHCCCCHHHHHCCCC
GGPAELRSPRRIRRALRRRRRRLDDPELPQPVPVPERLSDGPVRLADPRAAPARRRSHRG
CCCHHCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCHHCCCCCEEECCCCCCHHHHHHCCC
HDLLHRAEGRGARRARAAHPPVRGFLQRERNGDARRSRGIPRVPAGLRGPRGRMERHVPR
HHHHHHHCCCCHHHHHCCCCHHHHHHHHHCCCCHHHHCCCCCCCCCCCCCHHHHHHHHHH
RLALDRGPRRSRAPDRHPPADERREDRGRRAVHGPAPLLDRDDEAGARRRKERRMKTIDL
HHHHCCCCCCCCCCCCCCCCHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCH
ADIQAFLYRESRLLDDKAWDAWLDCYRADAVFWMPSWDDADALVTDPQREISLIYYPNRQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCCCCCEECCCCCCEEEEECCCCC
GLEDRVFRIKTERSSATVPDTRTSHNIANVERESADGDVHTVRFNWHTLSYRYKTVSSYF
CCHHHHEEEEECCCCCCCCCCCCCCCHHHHHHCCCCCCEEEEEEEEEEEEEHHHHHHHHH
GMSRYAIDFSGDAPKIVSKYVVLKNDYINQLIDIYHI
CCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
PQFHLHTSRISIRYSISVGRTVLPATILNLTALSSSTIPAKHRRFTRGFPCDARRGASP
CCCEEEECEEEEEEEECCCCHHHHHHHHHHHHHCCCCCCHHHHHHCCCCCCCCCCCCCC
DRASARAPPPIREPIRRRPHDPDRPGPPCDAAPHRRFPRRRQGARRLPAAPQRVHRRNAV
CCCCCCCCCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHCCCCCHHHHHHHHHH
RARDEAHLRRQLDLSRAREPAPERERLLHDHDRPPADRDRAQPPRRAERVRQRLHAPRRD
HHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHCCCCCC
AVPPQARQPRELHVPVPRLDVQQRRQAAQSEGPRRSRLPGLLQPRRLARSEESRALRELS
CCCCCCCCCCEEECCCCCCCHHHHHHHHHHCCCCHHCCCCCCCHHHHHHHHHHHHHHHHH
RLPVRQPEPRSRAARRASRRCRAHHRHDRRSVGGRPRGAARLVDVHVRRQLEAHRRERRG
HCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCC
RLPRIGRSLELRGDRQPPQDRRAARRHDPRDGRGQLGPSGRRLLRVRSRPHAAVDALGEP
CCCCCCCCEEECCCCCCCHHHHHHHHCCCCCCCCCCCCCHHHHHHHHCCCCHHHHHCCCC
GGPAELRSPRRIRRALRRRRRRLDDPELPQPVPVPERLSDGPVRLADPRAAPARRRSHRG
CCCHHCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCHHCCCCCEEECCCCCCHHHHHHCCC
HDLLHRAEGRGARRARAAHPPVRGFLQRERNGDARRSRGIPRVPAGLRGPRGRMERHVPR
HHHHHHHCCCCHHHHHCCCCHHHHHHHHHCCCCHHHHCCCCCCCCCCCCCHHHHHHHHHH
RLALDRGPRRSRAPDRHPPADERREDRGRRAVHGPAPLLDRDDEAGARRRKERRMKTIDL
HHHHCCCCCCCCCCCCCCCCHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCH
ADIQAFLYRESRLLDDKAWDAWLDCYRADAVFWMPSWDDADALVTDPQREISLIYYPNRQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCCCCCEECCCCCCEEEEECCCCC
GLEDRVFRIKTERSSATVPDTRTSHNIANVERESADGDVHTVRFNWHTLSYRYKTVSSYF
CCHHHHEEEEECCCCCCCCCCCCCCCHHHHHHCCCCCCEEEEEEEEEEEEEHHHHHHHHH
GMSRYAIDFSGDAPKIVSKYVVLKNDYINQLIDIYHI
CCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1885518 [H]