Click here to switch to the map view.

The map label for this gene is pyk [H]

Identifier: 76818303

GI number: 76818303

Start: 2002722

End: 2004176

Strand: Direct

Name: pyk [H]

Synonym: BURPS1710b_A1638

Alternate gene names: 76818303

Gene position: 2002722-2004176 (Clockwise)

Preceding gene: 76819073

Following gene: 76819080

Centisome position: 62.94

GC content: 70.24

Gene sequence:

>1455_bases
ATGCGAAACGAAAGCCATACCGCCCGCGAACGCCGCCGTTCGACGAAGATCGTCGCGACGCTCGGCCCGTCGAGTTCGAC
CGAGACCGCGATCGAGGCGCTCGCGCGCGCCGGCGCCGACGTGTTCCGTCTGAACTTCAGCCACGGCACGCATGCCGACC
ACGCGTTGCGCCACGCGGCCGTGCGCGCGATCGAGGCGCGCATCGGGCATCCGATCGGCGTCCTGCTCGATCTGCAGGGC
CCGAAGCTGCGGGTCGGGCAGTTCGCGAGCGGCCGCGCGCAGATCGTGAAGGGCCGGCCGTTCGTGTTCGACAGGGACCC
GGCGCCCGGCGACGCGCGGCGCGTGTCGCTGCCGCATCCGGAGATCTTCGACGCCGCGCGCCCCGGCCATCTGCTGCTTG
TCGACGACGGCAAGCTGCGGTTTCGCGTCGACGCGGTTTCATCGGCGCGCATCGAGACGACCGCGCTGCTCGACGGGATC
GTGTCCGATCGCAAGGGCGTGAGCGTGCCCGATGCGACGCTCGCGATTCCGGCGCTATCCGCGAAGGATCGCGACGATCT
CGAATTCGGGTTGTCGCTCGGCGTCGACTGGGTCGCGCTGTCGTTCGTGCAGACCGCGCAGGACGTGCGCGACGCGCGCG
CGTTGATCGGCGCGCGCGCGGCGATCGTCGCGAAGATCGAGAAGCCGCAGGCGGTCGCCAACATCGCCGAGATCGTCGAC
GCGGCCGATGCGGTGATGGTCGCGCGCGGCGATCTGGGCGTCGAGATGTCGCTCGAGGACGTGCCGAGCGTGCAGAAGCA
GATCATCCGGCTCGCGCGCGCGGCGGGCAAACCGGTGATCGTCGCGACGCAGATGCTCGAATCGATGACGCTCGCGCCGA
CGCCGACGCGCGCGGAGGCGTCCGACGTCGCCGCGGCCGTCTACGACGGCGCCGACGCGGTGATGCTGTCGGCGGAATCG
GCGTCGGGCCAGTATCCGGTCGAGGCGGTCGATTTCATGCGCAAGATCATTTCGACGACCGAGGCGGACCCGATTCAGCC
GCAGCTGATGAAGGCGATCGTCACCGCGCACGCGCCGAACGCGACCGACGCGATCGGCGCGGCGATCGAGGTGGTGTCGG
ATACGCTGCGGCTCGGCGTTGCCGTCACGTACACGGCGTCGGGCGCCACGGCGATACGTCTGTCGCGGCTGCGCCCGAGC
ACGGCGATCCTGAGCCTCACGCCGCGCGCCGAGATCGCGCGCCGGCTCACGCTCGCATGGGGCGTGCGCTCGCGTGTGGC
GGCGGAGGCGACCGGCATCGAGAACATCGTCGAGATCGCGATCGAAGCCGCGCACGCGGAAGGTTTCGCGACCGACGAAT
GTCCGATCGTCGTCGCCGCGGGCGTGCCGTTCGGCCATCCCGGCTCGACGAACCTGATGCGCATCGTCTGGCCGACCGAA
ATGACGCGACAATAG

Upstream 100 bases:

>100_bases
CCGCATCGCCCCGTGCACGCCGCCCGCATCCCGCGCCGCCCGTTGCCGGGCGGTGCGCTTCTCCGAACCCTCCGACTCAT
CTCACGCCCAGGAACCGGAT

Downstream 100 bases:

>100_bases
CGTCGAGCCGGTGTGCCGGCCCGCTTTGCCGGCCGCCTGGCGGACCGTTTCGCGGGCGGCTTCGCGCCCGCTTTGCAGAT
CGCTTCACAGGCTGTTTCGC

Product: pyruvate kinase

Products: NA

Alternate protein names: PK [H]

Number of amino acids: Translated: 484; Mature: 484

Protein sequence:

>484_residues
MRNESHTARERRRSTKIVATLGPSSSTETAIEALARAGADVFRLNFSHGTHADHALRHAAVRAIEARIGHPIGVLLDLQG
PKLRVGQFASGRAQIVKGRPFVFDRDPAPGDARRVSLPHPEIFDAARPGHLLLVDDGKLRFRVDAVSSARIETTALLDGI
VSDRKGVSVPDATLAIPALSAKDRDDLEFGLSLGVDWVALSFVQTAQDVRDARALIGARAAIVAKIEKPQAVANIAEIVD
AADAVMVARGDLGVEMSLEDVPSVQKQIIRLARAAGKPVIVATQMLESMTLAPTPTRAEASDVAAAVYDGADAVMLSAES
ASGQYPVEAVDFMRKIISTTEADPIQPQLMKAIVTAHAPNATDAIGAAIEVVSDTLRLGVAVTYTASGATAIRLSRLRPS
TAILSLTPRAEIARRLTLAWGVRSRVAAEATGIENIVEIAIEAAHAEGFATDECPIVVAAGVPFGHPGSTNLMRIVWPTE
MTRQ

Sequences:

>Translated_484_residues
MRNESHTARERRRSTKIVATLGPSSSTETAIEALARAGADVFRLNFSHGTHADHALRHAAVRAIEARIGHPIGVLLDLQG
PKLRVGQFASGRAQIVKGRPFVFDRDPAPGDARRVSLPHPEIFDAARPGHLLLVDDGKLRFRVDAVSSARIETTALLDGI
VSDRKGVSVPDATLAIPALSAKDRDDLEFGLSLGVDWVALSFVQTAQDVRDARALIGARAAIVAKIEKPQAVANIAEIVD
AADAVMVARGDLGVEMSLEDVPSVQKQIIRLARAAGKPVIVATQMLESMTLAPTPTRAEASDVAAAVYDGADAVMLSAES
ASGQYPVEAVDFMRKIISTTEADPIQPQLMKAIVTAHAPNATDAIGAAIEVVSDTLRLGVAVTYTASGATAIRLSRLRPS
TAILSLTPRAEIARRLTLAWGVRSRVAAEATGIENIVEIAIEAAHAEGFATDECPIVVAAGVPFGHPGSTNLMRIVWPTE
MTRQ
>Mature_484_residues
MRNESHTARERRRSTKIVATLGPSSSTETAIEALARAGADVFRLNFSHGTHADHALRHAAVRAIEARIGHPIGVLLDLQG
PKLRVGQFASGRAQIVKGRPFVFDRDPAPGDARRVSLPHPEIFDAARPGHLLLVDDGKLRFRVDAVSSARIETTALLDGI
VSDRKGVSVPDATLAIPALSAKDRDDLEFGLSLGVDWVALSFVQTAQDVRDARALIGARAAIVAKIEKPQAVANIAEIVD
AADAVMVARGDLGVEMSLEDVPSVQKQIIRLARAAGKPVIVATQMLESMTLAPTPTRAEASDVAAAVYDGADAVMLSAES
ASGQYPVEAVDFMRKIISTTEADPIQPQLMKAIVTAHAPNATDAIGAAIEVVSDTLRLGVAVTYTASGATAIRLSRLRPS
TAILSLTPRAEIARRLTLAWGVRSRVAAEATGIENIVEIAIEAAHAEGFATDECPIVVAAGVPFGHPGSTNLMRIVWPTE
MTRQ

Specific function: Glycolysis; final step. [C]

COG id: COG0469

COG function: function code G; Pyruvate kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pyruvate kinase family [H]

Homologues:

Organism=Homo sapiens, GI10835121, Length=489, Percent_Identity=37.8323108384458, Blast_Score=275, Evalue=6e-74,
Organism=Homo sapiens, GI32967597, Length=489, Percent_Identity=37.8323108384458, Blast_Score=274, Evalue=1e-73,
Organism=Homo sapiens, GI33286422, Length=489, Percent_Identity=35.7873210633947, Blast_Score=256, Evalue=4e-68,
Organism=Homo sapiens, GI33286420, Length=489, Percent_Identity=35.7873210633947, Blast_Score=256, Evalue=4e-68,
Organism=Homo sapiens, GI33286418, Length=494, Percent_Identity=36.2348178137652, Blast_Score=255, Evalue=8e-68,
Organism=Homo sapiens, GI310128732, Length=287, Percent_Identity=36.2369337979094, Blast_Score=159, Evalue=4e-39,
Organism=Homo sapiens, GI310128730, Length=287, Percent_Identity=36.2369337979094, Blast_Score=159, Evalue=4e-39,
Organism=Homo sapiens, GI310128736, Length=235, Percent_Identity=33.1914893617021, Blast_Score=118, Evalue=1e-26,
Organism=Homo sapiens, GI310128734, Length=235, Percent_Identity=33.1914893617021, Blast_Score=118, Evalue=1e-26,
Organism=Homo sapiens, GI310128738, Length=214, Percent_Identity=33.1775700934579, Blast_Score=102, Evalue=1e-21,
Organism=Escherichia coli, GI1787965, Length=471, Percent_Identity=36.0934182590234, Blast_Score=275, Evalue=6e-75,
Organism=Escherichia coli, GI1788160, Length=479, Percent_Identity=37.5782881002088, Blast_Score=271, Evalue=6e-74,
Organism=Caenorhabditis elegans, GI17544584, Length=486, Percent_Identity=34.3621399176955, Blast_Score=271, Evalue=6e-73,
Organism=Caenorhabditis elegans, GI17506829, Length=486, Percent_Identity=35.3909465020576, Blast_Score=251, Evalue=7e-67,
Organism=Caenorhabditis elegans, GI71984406, Length=486, Percent_Identity=35.3909465020576, Blast_Score=251, Evalue=7e-67,
Organism=Caenorhabditis elegans, GI17506831, Length=486, Percent_Identity=35.3909465020576, Blast_Score=251, Evalue=8e-67,
Organism=Caenorhabditis elegans, GI71984413, Length=486, Percent_Identity=35.3909465020576, Blast_Score=251, Evalue=8e-67,
Organism=Saccharomyces cerevisiae, GI6324923, Length=483, Percent_Identity=34.5755693581781, Blast_Score=248, Evalue=2e-66,
Organism=Saccharomyces cerevisiae, GI6319279, Length=434, Percent_Identity=36.8663594470046, Blast_Score=243, Evalue=4e-65,
Organism=Drosophila melanogaster, GI24648964, Length=486, Percent_Identity=36.0082304526749, Blast_Score=261, Evalue=7e-70,
Organism=Drosophila melanogaster, GI28571814, Length=486, Percent_Identity=36.0082304526749, Blast_Score=261, Evalue=9e-70,
Organism=Drosophila melanogaster, GI24648966, Length=390, Percent_Identity=36.1538461538462, Blast_Score=224, Evalue=1e-58,
Organism=Drosophila melanogaster, GI24581235, Length=503, Percent_Identity=29.4234592445328, Blast_Score=202, Evalue=4e-52,
Organism=Drosophila melanogaster, GI24646914, Length=241, Percent_Identity=38.1742738589212, Blast_Score=157, Evalue=2e-38,

Paralogues:

None

Copy number: 500 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 124 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001697
- InterPro:   IPR015813
- InterPro:   IPR011037
- InterPro:   IPR015794
- InterPro:   IPR018209
- InterPro:   IPR015793
- InterPro:   IPR015795
- InterPro:   IPR015806 [H]

Pfam domain/function: PF00224 PK; PF02887 PK_C [H]

EC number: =2.7.1.40 [H]

Molecular weight: Translated: 51236; Mature: 51236

Theoretical pI: Translated: 6.75; Mature: 6.75

Prosite motif: PS00110 PYRUVATE_KINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRNESHTARERRRSTKIVATLGPSSSTETAIEALARAGADVFRLNFSHGTHADHALRHAA
CCCCHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHH
VRAIEARIGHPIGVLLDLQGPKLRVGQFASGRAQIVKGRPFVFDRDPAPGDARRVSLPHP
HHHHHHHHCCCEEEEEECCCCCEEECCCCCCCEEEEECCCEEEECCCCCCCCEEECCCCC
EIFDAARPGHLLLVDDGKLRFRVDAVSSARIETTALLDGIVSDRKGVSVPDATLAIPALS
HHHCCCCCCEEEEEECCCEEEEEECCCCCCHHHHHHHHHHHCCCCCCCCCCCCEEEECCC
AKDRDDLEFGLSLGVDWVALSFVQTAQDVRDARALIGARAAIVAKIEKPQAVANIAEIVD
CCCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCHHHHHHHHHHH
AADAVMVARGDLGVEMSLEDVPSVQKQIIRLARAAGKPVIVATQMLESMTLAPTPTRAEA
HHCEEEEEECCCCCEEEHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHCCCCCCCCCCH
SDVAAAVYDGADAVMLSAESASGQYPVEAVDFMRKIISTTEADPIQPQLMKAIVTAHAPN
HHHHHHEECCCCEEEEEECCCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCC
ATDAIGAAIEVVSDTLRLGVAVTYTASGATAIRLSRLRPSTAILSLTPRAEIARRLTLAW
CHHHHHHHHHHHHHHHHCCEEEEEECCCCEEEEEECCCCCEEEEEECCHHHHHHHHHHHH
GVRSRVAAEATGIENIVEIAIEAAHAEGFATDECPIVVAAGVPFGHPGSTNLMRIVWPTE
HHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCCCCCCCCCEEEEEECCH
MTRQ
HCCC
>Mature Secondary Structure
MRNESHTARERRRSTKIVATLGPSSSTETAIEALARAGADVFRLNFSHGTHADHALRHAA
CCCCHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHH
VRAIEARIGHPIGVLLDLQGPKLRVGQFASGRAQIVKGRPFVFDRDPAPGDARRVSLPHP
HHHHHHHHCCCEEEEEECCCCCEEECCCCCCCEEEEECCCEEEECCCCCCCCEEECCCCC
EIFDAARPGHLLLVDDGKLRFRVDAVSSARIETTALLDGIVSDRKGVSVPDATLAIPALS
HHHCCCCCCEEEEEECCCEEEEEECCCCCCHHHHHHHHHHHCCCCCCCCCCCCEEEECCC
AKDRDDLEFGLSLGVDWVALSFVQTAQDVRDARALIGARAAIVAKIEKPQAVANIAEIVD
CCCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCHHHHHHHHHHH
AADAVMVARGDLGVEMSLEDVPSVQKQIIRLARAAGKPVIVATQMLESMTLAPTPTRAEA
HHCEEEEEECCCCCEEEHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHCCCCCCCCCCH
SDVAAAVYDGADAVMLSAESASGQYPVEAVDFMRKIISTTEADPIQPQLMKAIVTAHAPN
HHHHHHEECCCCEEEEEECCCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCC
ATDAIGAAIEVVSDTLRLGVAVTYTASGATAIRLSRLRPSTAILSLTPRAEIARRLTLAW
CHHHHHHHHHHHHHHHHCCEEEEEECCCCEEEEEECCCCCEEEEEECCHHHHHHHHHHHH
GVRSRVAAEATGIENIVEIAIEAAHAEGFATDECPIVVAAGVPFGHPGSTNLMRIVWPTE
HHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCCCCCCCCCEEEEEECCH
MTRQ
HCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA