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The map label for this gene is ispH

Identifier: 76818269

GI number: 76818269

Start: 1597081

End: 1598022

Strand: Direct

Name: ispH

Synonym: BURPS1710b_A1285

Alternate gene names: 76818269

Gene position: 1597081-1598022 (Clockwise)

Preceding gene: 76819824

Following gene: 76817760

Centisome position: 50.19

GC content: 67.73

Gene sequence:

>942_bases
ATGCGAGTCATCCTTGCCCAGCCTCGCGGCTTTTGTGCGGGGGTTGTCCGCGCGATCGAGATCGTCGAGCGCGCGCTGCA
ACAGAACGGCGCGCCGGTCTATGTACGTCACGAGATCGTCCATAATCGGCACGTAGTCGAAAACCTGAGAAATAAAGGGG
CGCGATTCGTTGAGGAACTCGACGAAGTGCCGCACGGCGCCGTGGCCATCTTCAGCGCGCACGGCGTCGCGCAGACCGTC
GAGCAGGATGCGCAGGCCCGCGGCCTCGACGTCCTCGACGCAACCTGCCCGCTCGTCACGAAGGTGCACGTGCAGGGGCG
TCAGTACGTCGCGGCCGGCCGCCGGCTGATCCTGATCGGCCACGCCGGCCATCCGGAAGTCGAAGGCACGATCGGGCAGA
TTCCGGCCGAGGTGATCCTCGTCCAGAGCGAGGCCGAGGTCGACACGCTGACGCTGCCGGCCGACACGCCCGTCGCGTAC
GTGACGCAGACGACGCTGTCGGTGGACGACACGCGCGGCATCATCGAGGCGCTGCAACGCCGGTTCACCGACATCGTCGG
CCCGGACACGCGCGACATCTGCTACGCGACGCAGAACCGCCAGGCGGCGGTGCGCGAGCTGAGCGAACAGGTCGACGTGC
TGCTCGTCGTCGGCGCGACGAACAGCTCGAACTCGAACCGGCTGCGCGAGATCGGCACCGAAAGCGGCGTGCCGAGCTAT
CTCGTCGCGGACGGCTCGGAAGTCCGCGCCGAATGGTTCGCGAACGCGCGCACGGTGGGCCTCACGGCCGGCGCGTCGGC
GCCCGAGGAAATGGTCGAGGACGTGATCGCCGCGCTGCGCGCGCTCGGTCCGCTCGAGGTCGCGACGATGTCGGGCCGCG
AGGAAAAAGTCGAATTCAAGCTGCCGGCGAAGCTCACGCAAGCTGTCGCCCGCGAAGTTTAA

Upstream 100 bases:

>100_bases
TGCCGGGCCCGTTCCAGCATTCATAACATCCAAGTTGGGGCGATCGGCCCGGTCTGCCGGCCGCTCGCACCCCGCGTCAG
TCGTCGGAGTTCGCCCAAAT

Downstream 100 bases:

>100_bases
GGAGGACATCCCTTGTCTATTCCGCTGCTCCAGCAAGTCCGCGTTGGCGCGTACATCATGCGCCAGCACCTGTCCGGCAA
CAAACGCTATCCGCTCGCGC

Product: 4-hydroxy-3-methylbut-2-enyl diphosphate reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 313; Mature: 313

Protein sequence:

>313_residues
MRVILAQPRGFCAGVVRAIEIVERALQQNGAPVYVRHEIVHNRHVVENLRNKGARFVEELDEVPHGAVAIFSAHGVAQTV
EQDAQARGLDVLDATCPLVTKVHVQGRQYVAAGRRLILIGHAGHPEVEGTIGQIPAEVILVQSEAEVDTLTLPADTPVAY
VTQTTLSVDDTRGIIEALQRRFTDIVGPDTRDICYATQNRQAAVRELSEQVDVLLVVGATNSSNSNRLREIGTESGVPSY
LVADGSEVRAEWFANARTVGLTAGASAPEEMVEDVIAALRALGPLEVATMSGREEKVEFKLPAKLTQAVAREV

Sequences:

>Translated_313_residues
MRVILAQPRGFCAGVVRAIEIVERALQQNGAPVYVRHEIVHNRHVVENLRNKGARFVEELDEVPHGAVAIFSAHGVAQTV
EQDAQARGLDVLDATCPLVTKVHVQGRQYVAAGRRLILIGHAGHPEVEGTIGQIPAEVILVQSEAEVDTLTLPADTPVAY
VTQTTLSVDDTRGIIEALQRRFTDIVGPDTRDICYATQNRQAAVRELSEQVDVLLVVGATNSSNSNRLREIGTESGVPSY
LVADGSEVRAEWFANARTVGLTAGASAPEEMVEDVIAALRALGPLEVATMSGREEKVEFKLPAKLTQAVAREV
>Mature_313_residues
MRVILAQPRGFCAGVVRAIEIVERALQQNGAPVYVRHEIVHNRHVVENLRNKGARFVEELDEVPHGAVAIFSAHGVAQTV
EQDAQARGLDVLDATCPLVTKVHVQGRQYVAAGRRLILIGHAGHPEVEGTIGQIPAEVILVQSEAEVDTLTLPADTPVAY
VTQTTLSVDDTRGIIEALQRRFTDIVGPDTRDICYATQNRQAAVRELSEQVDVLLVVGATNSSNSNRLREIGTESGVPSY
LVADGSEVRAEWFANARTVGLTAGASAPEEMVEDVIAALRALGPLEVATMSGREEKVEFKLPAKLTQAVAREV

Specific function: Converts 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate into isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP)

COG id: COG0761

COG function: function code IM; Penicillin tolerance protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispH family

Homologues:

Organism=Escherichia coli, GI1786212, Length=308, Percent_Identity=54.2207792207792, Blast_Score=337, Evalue=4e-94,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPH1_BURMA (Q629Z7)

Other databases:

- EMBL:   CP000011
- RefSeq:   YP_106485.1
- GeneID:   3086216
- GenomeReviews:   CP000011_GR
- KEGG:   bma:BMAA1962
- TIGR:   BMAA1962
- HOGENOM:   HBG335228
- OMA:   REENIEF
- PhylomeDB:   Q629Z7
- ProtClustDB:   PRK01045
- BioCyc:   BMAL243160:BMA_A1962-MONOMER
- BRENDA:   1.17.1.2
- HAMAP:   MF_00191
- InterPro:   IPR003451
- TIGRFAMs:   TIGR00216

Pfam domain/function: PF02401 LYTB

EC number: =1.17.1.2

Molecular weight: Translated: 33770; Mature: 33770

Theoretical pI: Translated: 4.99; Mature: 4.99

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVILAQPRGFCAGVVRAIEIVERALQQNGAPVYVRHEIVHNRHVVENLRNKGARFVEEL
CEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHH
DEVPHGAVAIFSAHGVAQTVEQDAQARGLDVLDATCPLVTKVHVQGRQYVAAGRRLILIG
HHCCCCEEEEEECCCHHHHHHHHHHHCCCCHHHCCCCHHEEEECCCCHHHHCCCEEEEEE
HAGHPEVEGTIGQIPAEVILVQSEAEVDTLTLPADTPVAYVTQTTLSVDDTRGIIEALQR
CCCCCCCCCHHHCCCEEEEEEECCCCCCEEECCCCCCEEEEEEEEECCCHHHHHHHHHHH
RFTDIVGPDTRDICYATQNRQAAVRELSEQVDVLLVVGATNSSNSNRLREIGTESGVPSY
HHHHHCCCCCCCEEEECCCHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHCCCCCCCCE
LVADGSEVRAEWFANARTVGLTAGASAPEEMVEDVIAALRALGPLEVATMSGREEKVEFK
EEECCHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHHCCCCEEEECCCCCCEEEEE
LPAKLTQAVAREV
CCHHHHHHHHHCC
>Mature Secondary Structure
MRVILAQPRGFCAGVVRAIEIVERALQQNGAPVYVRHEIVHNRHVVENLRNKGARFVEEL
CEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHH
DEVPHGAVAIFSAHGVAQTVEQDAQARGLDVLDATCPLVTKVHVQGRQYVAAGRRLILIG
HHCCCCEEEEEECCCHHHHHHHHHHHCCCCHHHCCCCHHEEEECCCCHHHHCCCEEEEEE
HAGHPEVEGTIGQIPAEVILVQSEAEVDTLTLPADTPVAYVTQTTLSVDDTRGIIEALQR
CCCCCCCCCHHHCCCEEEEEEECCCCCCEEECCCCCCEEEEEEEEECCCHHHHHHHHHHH
RFTDIVGPDTRDICYATQNRQAAVRELSEQVDVLLVVGATNSSNSNRLREIGTESGVPSY
HHHHHCCCCCCCEEEECCCHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHCCCCCCCCE
LVADGSEVRAEWFANARTVGLTAGASAPEEMVEDVIAALRALGPLEVATMSGREEKVEFK
EEECCHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHHCCCCEEEECCCCCCEEEEE
LPAKLTQAVAREV
CCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA