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The map label for this gene is rpoE3 [H]

Identifier: 76818123

GI number: 76818123

Start: 1896956

End: 1897651

Strand: Direct

Name: rpoE3 [H]

Synonym: BURPS1710b_A1567

Alternate gene names: 76818123

Gene position: 1896956-1897651 (Clockwise)

Preceding gene: 76818738

Following gene: 76817253

Centisome position: 59.62

GC content: 67.1

Gene sequence:

>696_bases
ATGACGACCGAGCCGCGATCGGGCGACATGCAGCCTTCGCGCGTGGACGACCGCGCGCTGGCCGCGCGGGTTGCCGGCGG
CGATCATGCGGCATTCGAGCAATTGATGCGCCGCTACAACCGGCGCCTCTACCGGCTTGCGCGCGCGACGTTGCGCGACG
ACGCGCAAGCCGAAGACGTGCTTCAGGAGGCCTATCTGGCCGCATTTCGCACCATCGGGCGCTTTCGCGGCGACGCATCG
CTGTCCACCTGGCTGTCGCGCCTCGTGCTCAACGCGTGCTTTACGCGCCAGCGCCGGGGCGCCCGGCGCGACAACATCGC
CCCGATGGTGAATACATCGTCCATCGCCGGCTTCGAGCGCACTACCATGGATGCGGACCTAACCTCCTCGCCCGATCATG
CACTCATGCGCACGGAGCTGCGTGCATTGCTCGAGCGCAAGCTCGATGCGTTGCCCGAGGCTTTTCGCCTCGTGTTCGTC
CTGCGTTGCGTGGAGGAGTTGAGCGTCGAGGAGACCGCGCAGTGTCTCGGCATACCGGAGGCCACCGTGCGATCGCGCCA
TTTTCGGGCGAAGCGCTTACTGCGCGAATCGCTCGCGCGGGAAATCGATTCGGCCGGGCGCGACGTGTTCTCGTTCGCCG
GCGAGCGCTGCGACCGCATCGTCCGCGGCGTACTGGCGAGGCTGATTTCGCCCTGA

Upstream 100 bases:

>100_bases
CGTATGTCGCGCGCAGCCCCGGGCGCTTTGCGTACGTCTGCGCGCTTCACCCCACCATGAAGGCGAATTTGATCGTCAAG
AGCAAGCGATGACCCGAACG

Downstream 100 bases:

>100_bases
TTTCGGGCACGCGGGCGGCGCTTGCCGCGTGCCGCCGCGCCGCCGGCACCGCACGGCAAGCGTCGGCAGGCGCCTGGATC
GACTGGCGGCCCGACGTACC

Product: RNA polymerase sigma factor

Products: NA

Alternate protein names: Sigma-30 [H]

Number of amino acids: Translated: 231; Mature: 230

Protein sequence:

>231_residues
MTTEPRSGDMQPSRVDDRALAARVAGGDHAAFEQLMRRYNRRLYRLARATLRDDAQAEDVLQEAYLAAFRTIGRFRGDAS
LSTWLSRLVLNACFTRQRRGARRDNIAPMVNTSSIAGFERTTMDADLTSSPDHALMRTELRALLERKLDALPEAFRLVFV
LRCVEELSVEETAQCLGIPEATVRSRHFRAKRLLRESLAREIDSAGRDVFSFAGERCDRIVRGVLARLISP

Sequences:

>Translated_231_residues
MTTEPRSGDMQPSRVDDRALAARVAGGDHAAFEQLMRRYNRRLYRLARATLRDDAQAEDVLQEAYLAAFRTIGRFRGDAS
LSTWLSRLVLNACFTRQRRGARRDNIAPMVNTSSIAGFERTTMDADLTSSPDHALMRTELRALLERKLDALPEAFRLVFV
LRCVEELSVEETAQCLGIPEATVRSRHFRAKRLLRESLAREIDSAGRDVFSFAGERCDRIVRGVLARLISP
>Mature_230_residues
TTEPRSGDMQPSRVDDRALAARVAGGDHAAFEQLMRRYNRRLYRLARATLRDDAQAEDVLQEAYLAAFRTIGRFRGDASL
STWLSRLVLNACFTRQRRGARRDNIAPMVNTSSIAGFERTTMDADLTSSPDHALMRTELRALLERKLDALPEAFRLVFVL
RCVEELSVEETAQCLGIPEATVRSRHFRAKRLLRESLAREIDSAGRDVFSFAGERCDRIVRGVLARLISP

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor regulates genes such as algD, involved in alginate biosynthesis [H]

COG id: COG1595

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit, sigma24 homolog

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family. ECF subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788926, Length=180, Percent_Identity=33.3333333333333, Blast_Score=97, Evalue=1e-21,

Paralogues:

None

Copy number: <10 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000838
- InterPro:   IPR007627
- InterPro:   IPR013249
- InterPro:   IPR014286
- InterPro:   IPR013325
- InterPro:   IPR013324 [H]

Pfam domain/function: PF04542 Sigma70_r2; PF08281 Sigma70_r4_2 [H]

EC number: NA

Molecular weight: Translated: 26141; Mature: 26010

Theoretical pI: Translated: 10.15; Mature: 10.15

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTEPRSGDMQPSRVDDRALAARVAGGDHAAFEQLMRRYNRRLYRLARATLRDDAQAEDV
CCCCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHH
LQEAYLAAFRTIGRFRGDASLSTWLSRLVLNACFTRQRRGARRDNIAPMVNTSSIAGFER
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHCCCHH
TTMDADLTSSPDHALMRTELRALLERKLDALPEAFRLVFVLRCVEELSVEETAQCLGIPE
HHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCH
ATVRSRHFRAKRLLRESLAREIDSAGRDVFSFAGERCDRIVRGVLARLISP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TTEPRSGDMQPSRVDDRALAARVAGGDHAAFEQLMRRYNRRLYRLARATLRDDAQAEDV
CCCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHH
LQEAYLAAFRTIGRFRGDASLSTWLSRLVLNACFTRQRRGARRDNIAPMVNTSSIAGFER
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHCCCHH
TTMDADLTSSPDHALMRTELRALLERKLDALPEAFRLVFVLRCVEELSVEETAQCLGIPE
HHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCH
ATVRSRHFRAKRLLRESLAREIDSAGRDVFSFAGERCDRIVRGVLARLISP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8432708; 8378309; 7961421; 10984043; 7737518 [H]