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The map label for this gene is bclA [H]

Identifier: 76817725

GI number: 76817725

Start: 2472907

End: 2474532

Strand: Direct

Name: bclA [H]

Synonym: BURPS1710b_A2033

Alternate gene names: 76817725

Gene position: 2472907-2474532 (Clockwise)

Preceding gene: 76818467

Following gene: 76818843

Centisome position: 77.72

GC content: 71.16

Gene sequence:

>1626_bases
ATGGACAACTTTTGCCGCACGAATCTGCCTGCACCGACCGATCTGCCGGAGTTCGTCTTCGAGTTGCCGGGCCTGCAGTA
TCCGGCGCGCATCAACTGCGCGGCGGCGCTGCTCGACGACGCGGTGACCCGCCGGGGCTGGGGCGAGCGCGTCGCGATCA
GGACCGAGTCCGGTGCCGCCTGGTCGTATCGCGCGCTGTTCGAGCTGAGCAACCGGATCGCCAACCTGCTGGTGCGCGAC
GGCGGGCTCGTGCCGGGCAACCGGGTGCTGCTGCACGGAACCAATCATCCGTTTCTCGCCGCCGCATGGTTCGCGATCGT
CAAGGCGGGCGGCGTCGTGGTGACGACGATGCCGCTGCTGCGCGCGGGCGAGCTGTCGAAAGTCATCGCGCAGGCGCAGG
TCACGCACGCGCTGTGCGAGGCGGCGGTGTCCGCCGAGTTGCGCGCCGCGATGGCGGCGGCGCCGGGCGTCGCGTTCGTG
CGGTACTACGAGACCGACGACGCGGCCGCGTTCGAGCCGCTGCTGCACGCGTGCCCGCGCACGTTCGAGCCGGTCGATAC
GCGCGCCGACGAGCCGTGCATCGTCGCGTTCACGTCGGGCACGACGGGGCGCCCGAAGGCGACCGTGCATTTTCATCGCG
ACGTGATGGCGATCTGCCATTGCTTTCCGCAGCACGTGCTGAAGCCGAACGCCGACGACGTGTTCTGCGGCTCGCCGCCG
CTCGCGTTCACGTTCGGGCTCGGCGCGCTGCTGCTGTTTCCGCTGAGCGTCGGCGCGAGCGTCGTGCTGCTGCAGCGGGC
GAAGCCGCAGCGGCTGCTCGCCGCGATCGGCGCGCATCGCGTGAGCATCCTCTTCACCGCGCCGGCCGCGTATCGCGCGA
TGCTCGACGAGCTCGGCGCGCACGACATCGCCAGCCTGCGCAAGTGCGTGTGCGCGGGCGAGGCGCTGCCGGCGCCGACG
CGCAACGCGTGGCTCGCGCGCACGGGCATTCGCATCATCGACGGCATCGGCGCGACCGAGATGCTGCACATCTTCGCGTC
CGCGGACGAAACGCAGGCGAAGGAAGGCGCGATCGGCAAGGCGGTGCCCGGCTACCGGCTCGCGATCCTCGACGAGCGCG
GCGAGCGCCTGCCGCCGTATCACGTCGGCCGTCTCGCGGTGCAGGGGCCGACCGGCTGCCGCTACCTGAACGATGCGCGG
CAGCGCGATTACGTGCGGCACGGCTGGAACCTGACGGGCGACGCCGCCTACCTCGACGAGGACGGCTACCTGTTCTACCA
GTCGCGCGCCGACGACCTGATCATCAGCCTCGGCTACACCATCTCGCCCGCCGAGGTGGAGGAGGCGCTGCTGAGCCACG
CGGACGTGCTCGAGTGCGGTGTTGTCGGCGCGCCCGACGGGCGAGGCGGCACGCTCGTGTGCGCGCACGTGGTGCCGCGG
CCCGGCGTGCACGGCTGCGATGCGCTGACGGCCGCGTTGCAGCAGCACGTGAAGGCGCGGATCGCGCCGTACAAGTATCC
GCGGCGCATCGAGTATCACGCGGCCGGGCTGCCGCGCAACGATTCCGGCAAGCTGCAGCGCTTCAAGCTGCGGCAGGCGG
CCGAGGAAGACGTGCAGGCGGCCTGA

Upstream 100 bases:

>100_bases
TTCGAACGGTTTCGGCGAATCGCGACGCCGGCGCATGCACGTTCGGCGCGCGGCGCCGAATCATCCTGGTTTCTCAGTCA
ACTTCTCGTGAGCACTGACG

Downstream 100 bases:

>100_bases
AGGCCGCGCGGGCGATGCGGCCCGAACGCCGCGCGCGGCGGGCGATCGGCGCCGGCATCGACCAACCCATTCGATAACGA
GGCGACATGAAAATACTGGG

Product: 2-aminobenzoate-CoA ligase

Products: NA

Alternate protein names: Benzoyl-CoA synthetase [H]

Number of amino acids: Translated: 541; Mature: 541

Protein sequence:

>541_residues
MDNFCRTNLPAPTDLPEFVFELPGLQYPARINCAAALLDDAVTRRGWGERVAIRTESGAAWSYRALFELSNRIANLLVRD
GGLVPGNRVLLHGTNHPFLAAAWFAIVKAGGVVVTTMPLLRAGELSKVIAQAQVTHALCEAAVSAELRAAMAAAPGVAFV
RYYETDDAAAFEPLLHACPRTFEPVDTRADEPCIVAFTSGTTGRPKATVHFHRDVMAICHCFPQHVLKPNADDVFCGSPP
LAFTFGLGALLLFPLSVGASVVLLQRAKPQRLLAAIGAHRVSILFTAPAAYRAMLDELGAHDIASLRKCVCAGEALPAPT
RNAWLARTGIRIIDGIGATEMLHIFASADETQAKEGAIGKAVPGYRLAILDERGERLPPYHVGRLAVQGPTGCRYLNDAR
QRDYVRHGWNLTGDAAYLDEDGYLFYQSRADDLIISLGYTISPAEVEEALLSHADVLECGVVGAPDGRGGTLVCAHVVPR
PGVHGCDALTAALQQHVKARIAPYKYPRRIEYHAAGLPRNDSGKLQRFKLRQAAEEDVQAA

Sequences:

>Translated_541_residues
MDNFCRTNLPAPTDLPEFVFELPGLQYPARINCAAALLDDAVTRRGWGERVAIRTESGAAWSYRALFELSNRIANLLVRD
GGLVPGNRVLLHGTNHPFLAAAWFAIVKAGGVVVTTMPLLRAGELSKVIAQAQVTHALCEAAVSAELRAAMAAAPGVAFV
RYYETDDAAAFEPLLHACPRTFEPVDTRADEPCIVAFTSGTTGRPKATVHFHRDVMAICHCFPQHVLKPNADDVFCGSPP
LAFTFGLGALLLFPLSVGASVVLLQRAKPQRLLAAIGAHRVSILFTAPAAYRAMLDELGAHDIASLRKCVCAGEALPAPT
RNAWLARTGIRIIDGIGATEMLHIFASADETQAKEGAIGKAVPGYRLAILDERGERLPPYHVGRLAVQGPTGCRYLNDAR
QRDYVRHGWNLTGDAAYLDEDGYLFYQSRADDLIISLGYTISPAEVEEALLSHADVLECGVVGAPDGRGGTLVCAHVVPR
PGVHGCDALTAALQQHVKARIAPYKYPRRIEYHAAGLPRNDSGKLQRFKLRQAAEEDVQAA
>Mature_541_residues
MDNFCRTNLPAPTDLPEFVFELPGLQYPARINCAAALLDDAVTRRGWGERVAIRTESGAAWSYRALFELSNRIANLLVRD
GGLVPGNRVLLHGTNHPFLAAAWFAIVKAGGVVVTTMPLLRAGELSKVIAQAQVTHALCEAAVSAELRAAMAAAPGVAFV
RYYETDDAAAFEPLLHACPRTFEPVDTRADEPCIVAFTSGTTGRPKATVHFHRDVMAICHCFPQHVLKPNADDVFCGSPP
LAFTFGLGALLLFPLSVGASVVLLQRAKPQRLLAAIGAHRVSILFTAPAAYRAMLDELGAHDIASLRKCVCAGEALPAPT
RNAWLARTGIRIIDGIGATEMLHIFASADETQAKEGAIGKAVPGYRLAILDERGERLPPYHVGRLAVQGPTGCRYLNDAR
QRDYVRHGWNLTGDAAYLDEDGYLFYQSRADDLIISLGYTISPAEVEEALLSHADVLECGVVGAPDGRGGTLVCAHVVPR
PGVHGCDALTAALQQHVKARIAPYKYPRRIEYHAAGLPRNDSGKLQRFKLRQAAEEDVQAA

Specific function: Catalyzes the ligation of benzoate and CoA to form benzoyl-CoA at the expense of ATP. The enzyme also ligates 2- aminobenzoate and CoA. The enzyme shows activity toward a number of benzoate derivates [H]

COG id: COG0365

COG function: function code I; Acyl-coenzyme A synthetases/AMP-(fatty) acid ligases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family. Benzoate-CoA ligase subfamily [H]

Homologues:

Organism=Homo sapiens, GI42544132, Length=536, Percent_Identity=28.1716417910448, Blast_Score=214, Evalue=2e-55,
Organism=Homo sapiens, GI38505220, Length=489, Percent_Identity=30.879345603272, Blast_Score=208, Evalue=1e-53,
Organism=Homo sapiens, GI58082049, Length=541, Percent_Identity=29.9445471349353, Blast_Score=204, Evalue=2e-52,
Organism=Homo sapiens, GI157311624, Length=541, Percent_Identity=29.3900184842884, Blast_Score=199, Evalue=4e-51,
Organism=Homo sapiens, GI157311622, Length=541, Percent_Identity=29.3900184842884, Blast_Score=199, Evalue=4e-51,
Organism=Homo sapiens, GI122937307, Length=534, Percent_Identity=29.4007490636704, Blast_Score=198, Evalue=1e-50,
Organism=Homo sapiens, GI115511026, Length=531, Percent_Identity=27.4952919020716, Blast_Score=192, Evalue=5e-49,
Organism=Homo sapiens, GI187761345, Length=541, Percent_Identity=26.6173752310536, Blast_Score=137, Evalue=3e-32,
Organism=Homo sapiens, GI187761343, Length=541, Percent_Identity=26.6173752310536, Blast_Score=137, Evalue=3e-32,
Organism=Homo sapiens, GI8923896, Length=378, Percent_Identity=31.4814814814815, Blast_Score=123, Evalue=4e-28,
Organism=Homo sapiens, GI253970489, Length=378, Percent_Identity=31.4814814814815, Blast_Score=123, Evalue=4e-28,
Organism=Homo sapiens, GI13375727, Length=550, Percent_Identity=25.2727272727273, Blast_Score=122, Evalue=8e-28,
Organism=Homo sapiens, GI195947378, Length=400, Percent_Identity=25, Blast_Score=111, Evalue=2e-24,
Organism=Homo sapiens, GI156151445, Length=503, Percent_Identity=23.8568588469185, Blast_Score=110, Evalue=3e-24,
Organism=Homo sapiens, GI28416953, Length=566, Percent_Identity=24.9116607773852, Blast_Score=102, Evalue=1e-21,
Organism=Homo sapiens, GI42544134, Length=387, Percent_Identity=22.2222222222222, Blast_Score=99, Evalue=9e-21,
Organism=Escherichia coli, GI145693145, Length=508, Percent_Identity=26.7716535433071, Blast_Score=149, Evalue=5e-37,
Organism=Escherichia coli, GI1788107, Length=557, Percent_Identity=24.7755834829443, Blast_Score=138, Evalue=9e-34,
Organism=Escherichia coli, GI1790505, Length=557, Percent_Identity=25.4937163375224, Blast_Score=133, Evalue=3e-32,
Organism=Escherichia coli, GI221142682, Length=512, Percent_Identity=25.1953125, Blast_Score=110, Evalue=2e-25,
Organism=Escherichia coli, GI1789201, Length=366, Percent_Identity=27.0491803278689, Blast_Score=105, Evalue=8e-24,
Organism=Escherichia coli, GI1786529, Length=552, Percent_Identity=25.5434782608696, Blast_Score=105, Evalue=1e-23,
Organism=Escherichia coli, GI1786810, Length=524, Percent_Identity=24.8091603053435, Blast_Score=95, Evalue=1e-20,
Organism=Escherichia coli, GI1786801, Length=487, Percent_Identity=24.6406570841889, Blast_Score=65, Evalue=8e-12,
Organism=Caenorhabditis elegans, GI71982997, Length=568, Percent_Identity=26.4084507042254, Blast_Score=124, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI71983001, Length=568, Percent_Identity=26.4084507042254, Blast_Score=124, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI17531443, Length=480, Percent_Identity=24.375, Blast_Score=119, Evalue=5e-27,
Organism=Caenorhabditis elegans, GI17538037, Length=508, Percent_Identity=24.4094488188976, Blast_Score=114, Evalue=1e-25,
Organism=Caenorhabditis elegans, GI17557194, Length=510, Percent_Identity=25.4901960784314, Blast_Score=114, Evalue=1e-25,
Organism=Caenorhabditis elegans, GI71985884, Length=350, Percent_Identity=27.7142857142857, Blast_Score=113, Evalue=3e-25,
Organism=Caenorhabditis elegans, GI17560308, Length=502, Percent_Identity=25.6972111553785, Blast_Score=110, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI71994690, Length=370, Percent_Identity=24.8648648648649, Blast_Score=107, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI71994703, Length=370, Percent_Identity=24.8648648648649, Blast_Score=107, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI71994694, Length=370, Percent_Identity=24.8648648648649, Blast_Score=106, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI17558820, Length=341, Percent_Identity=26.6862170087977, Blast_Score=106, Evalue=3e-23,
Organism=Caenorhabditis elegans, GI17559526, Length=371, Percent_Identity=28.8409703504043, Blast_Score=104, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI32563687, Length=484, Percent_Identity=23.3471074380165, Blast_Score=100, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI17560140, Length=352, Percent_Identity=25, Blast_Score=82, Evalue=9e-16,
Organism=Caenorhabditis elegans, GI71996755, Length=466, Percent_Identity=22.7467811158798, Blast_Score=79, Evalue=5e-15,
Organism=Saccharomyces cerevisiae, GI6323182, Length=401, Percent_Identity=27.930174563591, Blast_Score=107, Evalue=4e-24,
Organism=Saccharomyces cerevisiae, GI6319264, Length=538, Percent_Identity=27.5092936802974, Blast_Score=107, Evalue=6e-24,
Organism=Saccharomyces cerevisiae, GI6319699, Length=348, Percent_Identity=25.8620689655172, Blast_Score=98, Evalue=3e-21,
Organism=Drosophila melanogaster, GI24667955, Length=572, Percent_Identity=25.5244755244755, Blast_Score=125, Evalue=1e-28,
Organism=Drosophila melanogaster, GI62472339, Length=533, Percent_Identity=25.328330206379, Blast_Score=120, Evalue=3e-27,
Organism=Drosophila melanogaster, GI21355181, Length=512, Percent_Identity=25.5859375, Blast_Score=119, Evalue=5e-27,
Organism=Drosophila melanogaster, GI24667959, Length=372, Percent_Identity=27.6881720430108, Blast_Score=117, Evalue=1e-26,
Organism=Drosophila melanogaster, GI24581924, Length=368, Percent_Identity=27.7173913043478, Blast_Score=113, Evalue=3e-25,
Organism=Drosophila melanogaster, GI18859661, Length=363, Percent_Identity=25.8953168044077, Blast_Score=102, Evalue=5e-22,
Organism=Drosophila melanogaster, GI21355225, Length=522, Percent_Identity=23.1800766283525, Blast_Score=97, Evalue=2e-20,
Organism=Drosophila melanogaster, GI21356441, Length=360, Percent_Identity=25.2777777777778, Blast_Score=96, Evalue=5e-20,
Organism=Drosophila melanogaster, GI19922652, Length=377, Percent_Identity=26.790450928382, Blast_Score=90, Evalue=3e-18,
Organism=Drosophila melanogaster, GI281365686, Length=320, Percent_Identity=23.4375, Blast_Score=89, Evalue=1e-17,
Organism=Drosophila melanogaster, GI24656500, Length=404, Percent_Identity=24.5049504950495, Blast_Score=85, Evalue=1e-16,
Organism=Drosophila melanogaster, GI24648260, Length=358, Percent_Identity=26.8156424581006, Blast_Score=82, Evalue=8e-16,
Organism=Drosophila melanogaster, GI24648257, Length=321, Percent_Identity=27.4143302180685, Blast_Score=82, Evalue=9e-16,
Organism=Drosophila melanogaster, GI20130357, Length=376, Percent_Identity=25, Blast_Score=80, Evalue=4e-15,
Organism=Drosophila melanogaster, GI24653035, Length=496, Percent_Identity=22.5806451612903, Blast_Score=77, Evalue=3e-14,
Organism=Drosophila melanogaster, GI21356947, Length=499, Percent_Identity=23.0460921843687, Blast_Score=77, Evalue=4e-14,
Organism=Drosophila melanogaster, GI21358303, Length=339, Percent_Identity=24.7787610619469, Blast_Score=75, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24648253, Length=418, Percent_Identity=21.7703349282297, Blast_Score=74, Evalue=4e-13,
Organism=Drosophila melanogaster, GI24648255, Length=418, Percent_Identity=22.0095693779904, Blast_Score=73, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000873
- InterPro:   IPR011957 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =6.2.1.25 [H]

Molecular weight: Translated: 58326; Mature: 58326

Theoretical pI: Translated: 7.24; Mature: 7.24

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDNFCRTNLPAPTDLPEFVFELPGLQYPARINCAAALLDDAVTRRGWGERVAIRTESGAA
CCCCCCCCCCCCCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEECCCCC
WSYRALFELSNRIANLLVRDGGLVPGNRVLLHGTNHPFLAAAWFAIVKAGGVVVTTMPLL
CHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCHHHHHHHHHHHCCCEEEEEHHHH
RAGELSKVIAQAQVTHALCEAAVSAELRAAMAAAPGVAFVRYYETDDAAAFEPLLHACPR
HCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCHHHHHHHHHCCC
TFEPVDTRADEPCIVAFTSGTTGRPKATVHFHRDVMAICHCFPQHVLKPNADDVFCGSPP
CCCCCCCCCCCCEEEEEECCCCCCCCEEEEEHHHHHHHHHHHHHHHCCCCCCCEECCCCC
LAFTFGLGALLLFPLSVGASVVLLQRAKPQRLLAAIGAHRVSILFTAPAAYRAMLDELGA
HHHHHHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHCCCEEEEEEECCHHHHHHHHHHCC
HDIASLRKCVCAGEALPAPTRNAWLARTGIRIIDGIGATEMLHIFASADETQAKEGAIGK
HHHHHHHHHHHCCCCCCCCCCCCCHHHCCCHHEECCCHHHHHHHHHCCCHHHHHCCCCCC
AVPGYRLAILDERGERLPPYHVGRLAVQGPTGCRYLNDARQRDYVRHGWNLTGDAAYLDE
CCCCEEEEEEECCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHCCCCCCCCEEEECC
DGYLFYQSRADDLIISLGYTISPAEVEEALLSHADVLECGVVGAPDGRGGTLVCAHVVPR
CCEEEEECCCCCEEEEECCEECHHHHHHHHHHHCCHHHCCCEECCCCCCCCEEEEEECCC
PGVHGCDALTAALQQHVKARIAPYKYPRRIEYHAAGLPRNDSGKLQRFKLRQAAEEDVQA
CCCCCHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHCC
A
C
>Mature Secondary Structure
MDNFCRTNLPAPTDLPEFVFELPGLQYPARINCAAALLDDAVTRRGWGERVAIRTESGAA
CCCCCCCCCCCCCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEECCCCC
WSYRALFELSNRIANLLVRDGGLVPGNRVLLHGTNHPFLAAAWFAIVKAGGVVVTTMPLL
CHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCHHHHHHHHHHHCCCEEEEEHHHH
RAGELSKVIAQAQVTHALCEAAVSAELRAAMAAAPGVAFVRYYETDDAAAFEPLLHACPR
HCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCHHHHHHHHHCCC
TFEPVDTRADEPCIVAFTSGTTGRPKATVHFHRDVMAICHCFPQHVLKPNADDVFCGSPP
CCCCCCCCCCCCEEEEEECCCCCCCCEEEEEHHHHHHHHHHHHHHHCCCCCCCEECCCCC
LAFTFGLGALLLFPLSVGASVVLLQRAKPQRLLAAIGAHRVSILFTAPAAYRAMLDELGA
HHHHHHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHCCCEEEEEEECCHHHHHHHHHHCC
HDIASLRKCVCAGEALPAPTRNAWLARTGIRIIDGIGATEMLHIFASADETQAKEGAIGK
HHHHHHHHHHHCCCCCCCCCCCCCHHHCCCHHEECCCHHHHHHHHHCCCHHHHHCCCCCC
AVPGYRLAILDERGERLPPYHVGRLAVQGPTGCRYLNDARQRDYVRHGWNLTGDAAYLDE
CCCCEEEEEEECCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHCCCCCCCCEEEECC
DGYLFYQSRADDLIISLGYTISPAEVEEALLSHADVLECGVVGAPDGRGGTLVCAHVVPR
CCEEEEECCCCCEEEEECCEECHHHHHHHHHHHCCHHHCCCEECCCCCCCCEEEEEECCC
PGVHGCDALTAALQQHVKARIAPYKYPRRIEYHAAGLPRNDSGKLQRFKLRQAAEEDVQA
CCCCCHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHCC
A
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12897012 [H]