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The map label for this gene is ynfL [H]

Identifier: 76817414

GI number: 76817414

Start: 2131340

End: 2132221

Strand: Direct

Name: ynfL [H]

Synonym: BURPS1710b_A1746

Alternate gene names: 76817414

Gene position: 2131340-2132221 (Clockwise)

Preceding gene: 76817377

Following gene: 76817836

Centisome position: 66.99

GC content: 74.83

Gene sequence:

>882_bases
ATGGATCGCCTCGAACTGCGCCATGTGCGTGCGTTTCTCTGCGTCGCGCGGCACCTGCACTTCGCCCGCGCGGCGGACGA
GCTCGACATCGCGCCGCCCGCGCTGACGCGGCTCGTCCAGGACGCGGAGCGGCTTCTCGGCGTGCGGCTCTTTCACCGCA
CGCGGCGCTCGGTCGCGCTGAGCGCCGCGGGCGCGGCTTATCTCGGCGAAGCGCAGGCCGCGCTCGCGCATCTCGCGCGC
GGCCGCGAGCGGGCGGCGCTCGCCGAGCGCGGCGAAATCGGGCGGATCGAGCTCGGGTACGTATCGTCGGCGGCGTACGC
GGGGGTGCTGCAACGCACGGTGGGCGCGTTTCGCGACGCGCATCCGCGCATCCGCGTCGACGTGCGCGAAGTGCCGATGA
GCGAGGTTGCCGCGCGGCTCGACGCGGGCACGCTCGACGCCGCATACGTGCGCCCGCCGCTGCCGCTGCCGGACGGCGTG
TCGACGCACACCGTGCATCGCGATGTGTTCGTCGTCGCGGTGCACGGGCAATCGCCGCTCGCCGCGCGCGTATCGATTCG
GCCCGCCGCGCTCGCGGGCGAGCGCTTCGTCGTGCCCGAGCAGGCGCTCGGCACGCTGGAGGCGGCGCGCCGGGGGCGTT
TCGAGCCGATCGTCGACGCGCGGCCGGGCGCGCTCGTCGCGGTGCTCGCGCACGTGTCGGTGAGCGGCGGCGTCGCGATC
GTGCCGCAGGCGCTCGTCGGCTGCGTGTCGCTGCCGGGCGTCGCGTACCGGCCGATCGAGGGCAAGCCGATTGCGTCGGA
GGTGGCCGTTGCGTATCGCCGGCACGAGAAGGCGCCCGCGGTGCGCGCTTTCGTGCGGTTCGTGCGGCAGGCCGCGCTTT
AG

Upstream 100 bases:

>100_bases
ATGCGAAGGGGAGAGCGGCATGTTAGGCTGAGCGGCTGTTGCAAGTCATTGCCACAGAAATTCATCTTTATTGCATGAAA
AAGCAATAAAGGAGCCATCC

Downstream 100 bases:

>100_bases
CGCACGGGCGAGCGGCGCGCGGCGGCGGCGCTACGGCGGGATGCCGGATTCCGCGCGGCACGCGGCGCGGGCGCGCGGTG
CGTCACGTCCCCGCCGGCAA

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 293; Mature: 293

Protein sequence:

>293_residues
MDRLELRHVRAFLCVARHLHFARAADELDIAPPALTRLVQDAERLLGVRLFHRTRRSVALSAAGAAYLGEAQAALAHLAR
GRERAALAERGEIGRIELGYVSSAAYAGVLQRTVGAFRDAHPRIRVDVREVPMSEVAARLDAGTLDAAYVRPPLPLPDGV
STHTVHRDVFVVAVHGQSPLAARVSIRPAALAGERFVVPEQALGTLEAARRGRFEPIVDARPGALVAVLAHVSVSGGVAI
VPQALVGCVSLPGVAYRPIEGKPIASEVAVAYRRHEKAPAVRAFVRFVRQAAL

Sequences:

>Translated_293_residues
MDRLELRHVRAFLCVARHLHFARAADELDIAPPALTRLVQDAERLLGVRLFHRTRRSVALSAAGAAYLGEAQAALAHLAR
GRERAALAERGEIGRIELGYVSSAAYAGVLQRTVGAFRDAHPRIRVDVREVPMSEVAARLDAGTLDAAYVRPPLPLPDGV
STHTVHRDVFVVAVHGQSPLAARVSIRPAALAGERFVVPEQALGTLEAARRGRFEPIVDARPGALVAVLAHVSVSGGVAI
VPQALVGCVSLPGVAYRPIEGKPIASEVAVAYRRHEKAPAVRAFVRFVRQAAL
>Mature_293_residues
MDRLELRHVRAFLCVARHLHFARAADELDIAPPALTRLVQDAERLLGVRLFHRTRRSVALSAAGAAYLGEAQAALAHLAR
GRERAALAERGEIGRIELGYVSSAAYAGVLQRTVGAFRDAHPRIRVDVREVPMSEVAARLDAGTLDAAYVRPPLPLPDGV
STHTVHRDVFVVAVHGQSPLAARVSIRPAALAGERFVVPEQALGTLEAARRGRFEPIVDARPGALVAVLAHVSVSGGVAI
VPQALVGCVSLPGVAYRPIEGKPIASEVAVAYRRHEKAPAVRAFVRFVRQAAL

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787879, Length=289, Percent_Identity=32.5259515570934, Blast_Score=139, Evalue=2e-34,
Organism=Escherichia coli, GI2367136, Length=283, Percent_Identity=30.0353356890459, Blast_Score=108, Evalue=3e-25,
Organism=Escherichia coli, GI1788887, Length=295, Percent_Identity=28.135593220339, Blast_Score=105, Evalue=3e-24,
Organism=Escherichia coli, GI1790399, Length=324, Percent_Identity=24.3827160493827, Blast_Score=75, Evalue=4e-15,
Organism=Escherichia coli, GI145693105, Length=276, Percent_Identity=25.3623188405797, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI157672245, Length=292, Percent_Identity=26.027397260274, Blast_Score=65, Evalue=6e-12,
Organism=Escherichia coli, GI1790208, Length=281, Percent_Identity=24.1992882562278, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI1788748, Length=266, Percent_Identity=23.6842105263158, Blast_Score=62, Evalue=4e-11,
Organism=Escherichia coli, GI87081904, Length=180, Percent_Identity=26.1111111111111, Blast_Score=61, Evalue=8e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 31382; Mature: 31382

Theoretical pI: Translated: 11.06; Mature: 11.06

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDRLELRHVRAFLCVARHLHFARAADELDIAPPALTRLVQDAERLLGVRLFHRTRRSVAL
CCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SAAGAAYLGEAQAALAHLARGRERAALAERGEIGRIELGYVSSAAYAGVLQRTVGAFRDA
HHCCHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCEEEECCHHHHHHHHHHHHHHHHHHCC
HPRIRVDVREVPMSEVAARLDAGTLDAAYVRPPLPLPDGVSTHTVHRDVFVVAVHGQSPL
CCCEEEEHHHCCHHHHHHHHCCCCCCHHHCCCCCCCCCCCCCCEEECEEEEEEEECCCCC
AARVSIRPAALAGERFVVPEQALGTLEAARRGRFEPIVDARPGALVAVLAHVSVSGGVAI
EEEEEECHHHHCCCEEECCHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHCCCCEEH
VPQALVGCVSLPGVAYRPIEGKPIASEVAVAYRRHEKAPAVRAFVRFVRQAAL
HHHHHHHHHCCCCCEECCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure
MDRLELRHVRAFLCVARHLHFARAADELDIAPPALTRLVQDAERLLGVRLFHRTRRSVAL
CCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SAAGAAYLGEAQAALAHLARGRERAALAERGEIGRIELGYVSSAAYAGVLQRTVGAFRDA
HHCCHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCEEEECCHHHHHHHHHHHHHHHHHHCC
HPRIRVDVREVPMSEVAARLDAGTLDAAYVRPPLPLPDGVSTHTVHRDVFVVAVHGQSPL
CCCEEEEHHHCCHHHHHHHHCCCCCCHHHCCCCCCCCCCCCCCEEECEEEEEEEECCCCC
AARVSIRPAALAGERFVVPEQALGTLEAARRGRFEPIVDARPGALVAVLAHVSVSGGVAI
EEEEEECHHHHCCCEEECCHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHCCCCEEH
VPQALVGCVSLPGVAYRPIEGKPIASEVAVAYRRHEKAPAVRAFVRFVRQAAL
HHHHHHHHHCCCCCEECCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]