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The map label for this gene is rtcA

Identifier: 75909974

GI number: 75909974

Start: 4692020

End: 4693066

Strand: Direct

Name: rtcA

Synonym: Ava_3770

Alternate gene names: 75909974

Gene position: 4692020-4693066 (Clockwise)

Preceding gene: 75909973

Following gene: 75909975

Centisome position: 73.71

GC content: 47.76

Gene sequence:

>1047_bases
ATGATTGACATTGACGGTTCCTACGGCGAGGGAGGCGGGCAAGTCCTCCGCACTTCCCTGAGTTTAGCCGCCATCACTGG
TGAACCCATACGTATTGCCGGTATTCGCGCCGGACGCAGGAAGCCAGGATTAGCAGCACAACACTTAACAGCAGTACGTG
CAGCGGCGAGAATTTGTCATGGGGAATTGCAGGGTGATGCTTTAGGTTCCACGATGCTGGAATTTATCCCTGGTGGTGGG
GTAAAAGCGGGAAATTATATTTTTGATGTCAGCGAAGTACAGCAAGGTGGTTCAGCTGGCGCGATTACTCTAGTTTTACA
GACAATTCTCTTACCTTTAGCTTTAGCAGACGGTGATTCTCATATCACCTTACGGGGTGGGACTCATGTAATTTTTAGCC
CCACAGTCACCTACATTGAGCGAGTATATCTGCCAATGTTATGTCGTATGGGAATCAAGGCACAAGTTAAGTTAGGTGCT
TGGGGATGGTATCCCAGGGGTGGGGGAGAAGTAAATTTACAGGTGAAGGGAGGCTGTCAACTCTGCGGTCTTAACTTGCT
AGAAAGGGGAGAATTAAAGCGGGTACAAGGATTAGCTGTGGCGACAGAATTACCCGCACATATACCCCAACGTATGGCAA
ATCGGGCTGAGAATCTATTACGCACGGCTGGTTTGCGGGTATCTATGCAGGCATTACGTGAAAAAGGTGTTGCACCAGGG
GCGGGTATTTTTTTAACGGCTGAGTATTGTAATAGTTTGACGGGTTTTGGTGGCTTTGGACGTTTGCGGTTATCCTCTGA
GAAAGTAGCCGAGATAGCTTGCGGACAACTATTACAGTTCCATGAAACTGGTGCGCCTGTGGATGAACATCTAGCAGATC
AATTACTATTACCTGCTGCTTTAGCTTCCGAGTCCAGTCAGTATAGAGTGGCTGAGGTGAGTACACACTTGACTACTAAT
GCCGCAGTGATTGAAAAGTTTGGCTTAGGAAAGATTACAGTAAATCAAGCGGAAAGAGTGGTAGCGATCGCCTCTGATAA
AACGTAG

Upstream 100 bases:

>100_bases
AGGCAGGGGGAGAAGTCCCCAATCCCCAATCCCCAATCCCCAATCCCCAATCCCCAGTCCCCAAAAATTTTGAATTGATT
CATCGCCCAGACCACTGAGT

Downstream 100 bases:

>100_bases
CTTGTTAATATAATCTCTAATTAATTAGCAGCTATGAGCTAGAGACAGGATAATTCTCAAGGTTAAAAATCTACTTTTCT
CAATAAAAAATTTTCTATCT

Product: RNA 3'-terminal-phosphate cyclase

Products: NA

Alternate protein names: RNA cyclase; RNA-3'-phosphate cyclase

Number of amino acids: Translated: 348; Mature: 348

Protein sequence:

>348_residues
MIDIDGSYGEGGGQVLRTSLSLAAITGEPIRIAGIRAGRRKPGLAAQHLTAVRAAARICHGELQGDALGSTMLEFIPGGG
VKAGNYIFDVSEVQQGGSAGAITLVLQTILLPLALADGDSHITLRGGTHVIFSPTVTYIERVYLPMLCRMGIKAQVKLGA
WGWYPRGGGEVNLQVKGGCQLCGLNLLERGELKRVQGLAVATELPAHIPQRMANRAENLLRTAGLRVSMQALREKGVAPG
AGIFLTAEYCNSLTGFGGFGRLRLSSEKVAEIACGQLLQFHETGAPVDEHLADQLLLPAALASESSQYRVAEVSTHLTTN
AAVIEKFGLGKITVNQAERVVAIASDKT

Sequences:

>Translated_348_residues
MIDIDGSYGEGGGQVLRTSLSLAAITGEPIRIAGIRAGRRKPGLAAQHLTAVRAAARICHGELQGDALGSTMLEFIPGGG
VKAGNYIFDVSEVQQGGSAGAITLVLQTILLPLALADGDSHITLRGGTHVIFSPTVTYIERVYLPMLCRMGIKAQVKLGA
WGWYPRGGGEVNLQVKGGCQLCGLNLLERGELKRVQGLAVATELPAHIPQRMANRAENLLRTAGLRVSMQALREKGVAPG
AGIFLTAEYCNSLTGFGGFGRLRLSSEKVAEIACGQLLQFHETGAPVDEHLADQLLLPAALASESSQYRVAEVSTHLTTN
AAVIEKFGLGKITVNQAERVVAIASDKT
>Mature_348_residues
MIDIDGSYGEGGGQVLRTSLSLAAITGEPIRIAGIRAGRRKPGLAAQHLTAVRAAARICHGELQGDALGSTMLEFIPGGG
VKAGNYIFDVSEVQQGGSAGAITLVLQTILLPLALADGDSHITLRGGTHVIFSPTVTYIERVYLPMLCRMGIKAQVKLGA
WGWYPRGGGEVNLQVKGGCQLCGLNLLERGELKRVQGLAVATELPAHIPQRMANRAENLLRTAGLRVSMQALREKGVAPG
AGIFLTAEYCNSLTGFGGFGRLRLSSEKVAEIACGQLLQFHETGAPVDEHLADQLLLPAALASESSQYRVAEVSTHLTTN
AAVIEKFGLGKITVNQAERVVAIASDKT

Specific function: Catalyzes the conversion of 3'-phosphate to a 2',3'- cyclic phosphodiester at the end of RNA. The mechanism of action of the enzyme occurs in 3 steps:(A) adenylation of the enzyme by ATP; (B) transfer of adenylate to an RNA-N3'P to produce RNA- N3'PP5'A;

COG id: COG0430

COG function: function code A; RNA 3'-terminal phosphate cyclase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA 3'-terminal cyclase family. Type 1 subfamily

Homologues:

Organism=Homo sapiens, GI4506589, Length=352, Percent_Identity=32.6704545454545, Blast_Score=169, Evalue=3e-42,
Organism=Homo sapiens, GI195927044, Length=365, Percent_Identity=31.5068493150685, Blast_Score=162, Evalue=6e-40,
Organism=Escherichia coli, GI48994937, Length=331, Percent_Identity=41.3897280966767, Blast_Score=210, Evalue=1e-55,
Organism=Drosophila melanogaster, GI18543269, Length=334, Percent_Identity=33.2335329341317, Blast_Score=166, Evalue=2e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RTCA_ANAVT (Q3M6L1)

Other databases:

- EMBL:   CP000117
- RefSeq:   YP_324270.1
- ProteinModelPortal:   Q3M6L1
- SMR:   Q3M6L1
- STRING:   Q3M6L1
- GeneID:   3678974
- GenomeReviews:   CP000117_GR
- KEGG:   ava:Ava_3770
- NMPDR:   fig|240292.3.peg.3888
- eggNOG:   COG0430
- HOGENOM:   HBG683539
- OMA:   EIDGSYG
- PhylomeDB:   Q3M6L1
- ProtClustDB:   PRK04204
- BioCyc:   AVAR240292:AVA_3770-MONOMER
- GO:   GO:0005737
- GO:   GO:0005654
- HAMAP:   MF_00200
- InterPro:   IPR013791
- InterPro:   IPR000228
- InterPro:   IPR013796
- InterPro:   IPR020723
- InterPro:   IPR017770
- InterPro:   IPR013792
- Gene3D:   G3DSA:3.65.10.20
- PANTHER:   PTHR11096
- TIGRFAMs:   TIGR03399

Pfam domain/function: PF01137 RTC; PF05189 RTC_insert; SSF52913 RNA3'-term_phos_cycl_insert; SSF55205 RNA3'_cycl/enolpyr_transf_A/B

EC number: =6.5.1.4

Molecular weight: Translated: 36669; Mature: 36669

Theoretical pI: Translated: 8.37; Mature: 8.37

Prosite motif: PS01287 RTC

Important sites: ACT_SITE 316-316 BINDING 107-107

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIDIDGSYGEGGGQVLRTSLSLAAITGEPIRIAGIRAGRRKPGLAAQHLTAVRAAARICH
CEECCCCCCCCCHHHHHHHHEEEEECCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHH
GELQGDALGSTMLEFIPGGGVKAGNYIFDVSEVQQGGSAGAITLVLQTILLPLALADGDS
CCCCCCHHHHHHHHHCCCCCCCCCCEEEEHHHHHCCCCCCHHHHHHHHHHHHHEEECCCC
HITLRGGTHVIFSPTVTYIERVYLPMLCRMGIKAQVKLGAWGWYPRGGGEVNLQVKGGCQ
EEEEECCCEEEECCHHHHHHHHHHHHHHHCCCCEEEEECCEECCCCCCCEEEEEECCCCE
LCGLNLLERGELKRVQGLAVATELPAHIPQRMANRAENLLRTAGLRVSMQALREKGVAPG
EECCCHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCC
AGIFLTAEYCNSLTGFGGFGRLRLSSEKVAEIACGQLLQFHETGAPVDEHLADQLLLPAA
CCEEEEHHHHCCCCCCCCCCEEEECHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
LASESSQYRVAEVSTHLTTNAAVIEKFGLGKITVNQAERVVAIASDKT
HHCCCCCEEEEEHHHHHCCCHHHHHHCCCCEEEECCCCEEEEEECCCC
>Mature Secondary Structure
MIDIDGSYGEGGGQVLRTSLSLAAITGEPIRIAGIRAGRRKPGLAAQHLTAVRAAARICH
CEECCCCCCCCCHHHHHHHHEEEEECCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHH
GELQGDALGSTMLEFIPGGGVKAGNYIFDVSEVQQGGSAGAITLVLQTILLPLALADGDS
CCCCCCHHHHHHHHHCCCCCCCCCCEEEEHHHHHCCCCCCHHHHHHHHHHHHHEEECCCC
HITLRGGTHVIFSPTVTYIERVYLPMLCRMGIKAQVKLGAWGWYPRGGGEVNLQVKGGCQ
EEEEECCCEEEECCHHHHHHHHHHHHHHHCCCCEEEEECCEECCCCCCCEEEEEECCCCE
LCGLNLLERGELKRVQGLAVATELPAHIPQRMANRAENLLRTAGLRVSMQALREKGVAPG
EECCCHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCC
AGIFLTAEYCNSLTGFGGFGRLRLSSEKVAEIACGQLLQFHETGAPVDEHLADQLLLPAA
CCEEEEHHHHCCCCCCCCCCEEEECHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
LASESSQYRVAEVSTHLTTNAAVIEKFGLGKITVNQAERVVAIASDKT
HHCCCCCEEEEEHHHHHCCCHHHHHHCCCCEEEECCCCEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA