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The map label for this gene is cysS

Identifier: 75909922

GI number: 75909922

Start: 4627621

End: 4629081

Strand: Direct

Name: cysS

Synonym: Ava_3718

Alternate gene names: 75909922

Gene position: 4627621-4629081 (Clockwise)

Preceding gene: 75909915

Following gene: 75909923

Centisome position: 72.7

GC content: 45.31

Gene sequence:

>1461_bases
ATGACCCTAACGATTTACAATACCCTCACCCGCCGTCAAGAACCTTTGGAAACTGTAGAGCCAGGTAAGGTTAAGATGTA
TTGCTGCGGCGTGACAGTTTATGATTATTGCCATTTGGGTCATGCACGTTCCTATATAGTTTGGGATACGATTCGGCGTT
ATTTGATTTGGCGTGGTTTTGAAGTTAAATACATCCAAAATTTTACCGATATTGACGATAAGATTCTTAACCGTGCCAAA
GAACAAGGTTCAACAATGGCGGAGGTGTCAAACCGCTTTATTGATGCCTATTTTGCAGATATCCGCCGTTTAAATGTCCT
GGATGCGGATGAATATCCCCGCGTCACTGAGCATATTCCCGAAATTCATCAATTAATCCAAATTCTGGAAGAAAAAGGTC
TAGCTTACGCCGTTGGTGGGGATGTTTATTATCGCGTAGAACGCTTTCCCAGTTATGGCAAACTCTCTGGCCGGGAGTTG
GAACAAATGCAGGCTGGTGCTAGCGGTCGGGTAGATGTGGAAGACTCCGAACCAAAAAAGCAACATCCTTTTGATTTTGC
CTTGTGGAAAGCAGCAAAACCAGGGGAACCAGCGTGGGATTCTCCTTGGGGTCAAGGTCGTCCGGGATGGCATATTGAAT
GCTCGGCGATGATTCGTTCTAAGCTAGGGGCAACTATTGATATTCATGGTGGTGGTGGAGATTTGATTTTTCCCCACCAT
GAAAATGAAATTGCTCAGTCTGAAGCAGCTATGAATCAGCCATTGGCGCGTTATTGGACACATAATGGCATGGTGATGGT
GAATGGTCAGAAAATGTCAAAATCTCTGGGTAATTTCATCACCATTCGAGAATTATTAGATGGGGTGGGTAGTTGGAAAG
GCGACCCAGTAAACCCAATGGCTGTAAGATTGTTTGTCTTGCAGGCACATTACCGCAAGCCTCTAGATTTTACAGAAGAA
GCGATCGCCAACGCCGAAAATAGCTGGAAAACCCTCAAGGAAGGTTTGCTCTTTGGCTATCAATACGGTGAAAAGCTCGG
TTGGGGTCAAGAATCCGCAATTATTCCCGAACTCGCCACCCGTTTTCAAGAATTAGGCGACGATGACTTTAACTTCTCCG
GTGGCTTGGCGGTACTGTTTGAATTAGCCAAAGAACTCCGCCGCGAAGGTAATATCCTCGTCCACGAAGGAACCACCAAA
ACCCCCAGCGATGAACTCCAACGCCAATGGAATACCCTTGTCACATTAGGTAAAGTCTTGGGTTTAGAAGCAACACCAGA
TGATGAAACCCTGACACAACCAGGTCTAAGTGATACAGATATTGAAGCCCTAATTGAGCAAAGACAAGCAGCCAGAAAAA
ACAAGAACTTTAGCGAAAGCGATCGCATCCGTAATGAACTACAAGCACAAGGTGTAACCTTAATTGATAGCCCCCAAGGT
ACACGTTGGCATAGGAGTTAA

Upstream 100 bases:

>100_bases
GGTAGCGGAGATAGTCCAGAAAACACTAGCATAGGAATGCTGGAATATTTGTCCAGCTGCTTTATCCTATTATTGCCTAT
CTCTTCCAAGTTACCGTGTT

Downstream 100 bases:

>100_bases
AGGGGACTGGGGACTGGGGACTGGGAAAGAGAAAATTCACCCAATACCTAATCCCCAATACCCAATACCTAATCCCCAAT
ACCCAATCCCTATTCCCTAA

Product: cysteinyl-tRNA synthetase

Products: NA

Alternate protein names: Cysteine--tRNA ligase; CysRS

Number of amino acids: Translated: 486; Mature: 485

Protein sequence:

>486_residues
MTLTIYNTLTRRQEPLETVEPGKVKMYCCGVTVYDYCHLGHARSYIVWDTIRRYLIWRGFEVKYIQNFTDIDDKILNRAK
EQGSTMAEVSNRFIDAYFADIRRLNVLDADEYPRVTEHIPEIHQLIQILEEKGLAYAVGGDVYYRVERFPSYGKLSGREL
EQMQAGASGRVDVEDSEPKKQHPFDFALWKAAKPGEPAWDSPWGQGRPGWHIECSAMIRSKLGATIDIHGGGGDLIFPHH
ENEIAQSEAAMNQPLARYWTHNGMVMVNGQKMSKSLGNFITIRELLDGVGSWKGDPVNPMAVRLFVLQAHYRKPLDFTEE
AIANAENSWKTLKEGLLFGYQYGEKLGWGQESAIIPELATRFQELGDDDFNFSGGLAVLFELAKELRREGNILVHEGTTK
TPSDELQRQWNTLVTLGKVLGLEATPDDETLTQPGLSDTDIEALIEQRQAARKNKNFSESDRIRNELQAQGVTLIDSPQG
TRWHRS

Sequences:

>Translated_486_residues
MTLTIYNTLTRRQEPLETVEPGKVKMYCCGVTVYDYCHLGHARSYIVWDTIRRYLIWRGFEVKYIQNFTDIDDKILNRAK
EQGSTMAEVSNRFIDAYFADIRRLNVLDADEYPRVTEHIPEIHQLIQILEEKGLAYAVGGDVYYRVERFPSYGKLSGREL
EQMQAGASGRVDVEDSEPKKQHPFDFALWKAAKPGEPAWDSPWGQGRPGWHIECSAMIRSKLGATIDIHGGGGDLIFPHH
ENEIAQSEAAMNQPLARYWTHNGMVMVNGQKMSKSLGNFITIRELLDGVGSWKGDPVNPMAVRLFVLQAHYRKPLDFTEE
AIANAENSWKTLKEGLLFGYQYGEKLGWGQESAIIPELATRFQELGDDDFNFSGGLAVLFELAKELRREGNILVHEGTTK
TPSDELQRQWNTLVTLGKVLGLEATPDDETLTQPGLSDTDIEALIEQRQAARKNKNFSESDRIRNELQAQGVTLIDSPQG
TRWHRS
>Mature_485_residues
TLTIYNTLTRRQEPLETVEPGKVKMYCCGVTVYDYCHLGHARSYIVWDTIRRYLIWRGFEVKYIQNFTDIDDKILNRAKE
QGSTMAEVSNRFIDAYFADIRRLNVLDADEYPRVTEHIPEIHQLIQILEEKGLAYAVGGDVYYRVERFPSYGKLSGRELE
QMQAGASGRVDVEDSEPKKQHPFDFALWKAAKPGEPAWDSPWGQGRPGWHIECSAMIRSKLGATIDIHGGGGDLIFPHHE
NEIAQSEAAMNQPLARYWTHNGMVMVNGQKMSKSLGNFITIRELLDGVGSWKGDPVNPMAVRLFVLQAHYRKPLDFTEEA
IANAENSWKTLKEGLLFGYQYGEKLGWGQESAIIPELATRFQELGDDDFNFSGGLAVLFELAKELRREGNILVHEGTTKT
PSDELQRQWNTLVTLGKVLGLEATPDDETLTQPGLSDTDIEALIEQRQAARKNKNFSESDRIRNELQAQGVTLIDSPQGT
RWHRS

Specific function: Unknown

COG id: COG0215

COG function: function code J; Cysteinyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI13375695, Length=326, Percent_Identity=42.0245398773006, Blast_Score=242, Evalue=6e-64,
Organism=Homo sapiens, GI10835051, Length=250, Percent_Identity=43.2, Blast_Score=197, Evalue=1e-50,
Organism=Homo sapiens, GI62240992, Length=250, Percent_Identity=43.2, Blast_Score=197, Evalue=2e-50,
Organism=Homo sapiens, GI21269877, Length=250, Percent_Identity=43.2, Blast_Score=197, Evalue=2e-50,
Organism=Homo sapiens, GI303304987, Length=250, Percent_Identity=43.2, Blast_Score=197, Evalue=2e-50,
Organism=Escherichia coli, GI1786737, Length=494, Percent_Identity=44.1295546558704, Blast_Score=376, Evalue=1e-105,
Organism=Caenorhabditis elegans, GI17509797, Length=271, Percent_Identity=38.3763837638376, Blast_Score=181, Evalue=1e-45,
Organism=Caenorhabditis elegans, GI17509795, Length=271, Percent_Identity=38.3763837638376, Blast_Score=181, Evalue=1e-45,
Organism=Saccharomyces cerevisiae, GI6324082, Length=260, Percent_Identity=42.6923076923077, Blast_Score=182, Evalue=7e-47,
Organism=Drosophila melanogaster, GI19922218, Length=515, Percent_Identity=32.2330097087379, Blast_Score=218, Evalue=5e-57,
Organism=Drosophila melanogaster, GI19922364, Length=252, Percent_Identity=42.8571428571429, Blast_Score=194, Evalue=8e-50,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SYC_ANAVT (Q3M6R3)

Other databases:

- EMBL:   CP000117
- RefSeq:   YP_324218.1
- ProteinModelPortal:   Q3M6R3
- STRING:   Q3M6R3
- GeneID:   3678922
- GenomeReviews:   CP000117_GR
- KEGG:   ava:Ava_3718
- NMPDR:   fig|240292.3.peg.4871
- eggNOG:   COG0215
- HOGENOM:   HBG327651
- OMA:   DFDALNM
- PhylomeDB:   Q3M6R3
- ProtClustDB:   PRK00260
- BioCyc:   AVAR240292:AVA_3718-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00041_B
- InterPro:   IPR015803
- InterPro:   IPR015273
- InterPro:   IPR014729
- InterPro:   IPR009080
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR10890
- PRINTS:   PR00983
- SMART:   SM00840
- TIGRFAMs:   TIGR00435

Pfam domain/function: PF09190 DALR_2; PF01406 tRNA-synt_1e; SSF47323 tRNAsyn_1a_bind

EC number: =6.1.1.16

Molecular weight: Translated: 55153; Mature: 55022

Theoretical pI: Translated: 5.39; Mature: 5.39

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: BINDING 274-274

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLTIYNTLTRRQEPLETVEPGKVKMYCCGVTVYDYCHLGHARSYIVWDTIRRYLIWRGF
CEEEEEEHHHHCCCCHHHCCCCCEEEEEECCHHHHHHHHCCCCCCHHHHHHHHHHHHCCC
EVKYIQNFTDIDDKILNRAKEQGSTMAEVSNRFIDAYFADIRRLNVLDADEYPRVTEHIP
EEHHHHCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHH
EIHQLIQILEEKGLAYAVGGDVYYRVERFPSYGKLSGRELEQMQAGASGRVDVEDSEPKK
HHHHHHHHHHHCCCEEEECCCEEEEEECCCCCCCCCCHHHHHHHCCCCCCCCCCCCCCCC
QHPFDFALWKAAKPGEPAWDSPWGQGRPGWHIECSAMIRSKLGATIDIHGGGGDLIFPHH
CCCCCCEEECCCCCCCCCCCCCCCCCCCCCEEEHHHHHHHHCCCEEEEECCCCCEECCCC
ENEIAQSEAAMNQPLARYWTHNGMVMVNGQKMSKSLGNFITIRELLDGVGSWKGDPVNPM
CCHHHHHHHHHCCCHHHHHCCCCEEEEECHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHH
AVRLFVLQAHYRKPLDFTEEAIANAENSWKTLKEGLLFGYQYGEKLGWGQESAIIPELAT
HHHHHHHHHHHCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHH
RFQELGDDDFNFSGGLAVLFELAKELRREGNILVHEGTTKTPSDELQRQWNTLVTLGKVL
HHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHH
GLEATPDDETLTQPGLSDTDIEALIEQRQAARKNKNFSESDRIRNELQAQGVTLIDSPQG
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCEEEEECCCC
TRWHRS
CCCCCC
>Mature Secondary Structure 
TLTIYNTLTRRQEPLETVEPGKVKMYCCGVTVYDYCHLGHARSYIVWDTIRRYLIWRGF
EEEEEEHHHHCCCCHHHCCCCCEEEEEECCHHHHHHHHCCCCCCHHHHHHHHHHHHCCC
EVKYIQNFTDIDDKILNRAKEQGSTMAEVSNRFIDAYFADIRRLNVLDADEYPRVTEHIP
EEHHHHCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHH
EIHQLIQILEEKGLAYAVGGDVYYRVERFPSYGKLSGRELEQMQAGASGRVDVEDSEPKK
HHHHHHHHHHHCCCEEEECCCEEEEEECCCCCCCCCCHHHHHHHCCCCCCCCCCCCCCCC
QHPFDFALWKAAKPGEPAWDSPWGQGRPGWHIECSAMIRSKLGATIDIHGGGGDLIFPHH
CCCCCCEEECCCCCCCCCCCCCCCCCCCCCEEEHHHHHHHHCCCEEEEECCCCCEECCCC
ENEIAQSEAAMNQPLARYWTHNGMVMVNGQKMSKSLGNFITIRELLDGVGSWKGDPVNPM
CCHHHHHHHHHCCCHHHHHCCCCEEEEECHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHH
AVRLFVLQAHYRKPLDFTEEAIANAENSWKTLKEGLLFGYQYGEKLGWGQESAIIPELAT
HHHHHHHHHHHCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHH
RFQELGDDDFNFSGGLAVLFELAKELRREGNILVHEGTTKTPSDELQRQWNTLVTLGKVL
HHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHH
GLEATPDDETLTQPGLSDTDIEALIEQRQAARKNKNFSESDRIRNELQAQGVTLIDSPQG
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCEEEEECCCC
TRWHRS
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA