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The map label for this gene is folC [H]

Identifier: 75909888

GI number: 75909888

Start: 4589809

End: 4591059

Strand: Direct

Name: folC [H]

Synonym: Ava_3683

Alternate gene names: 75909888

Gene position: 4589809-4591059 (Clockwise)

Preceding gene: 75909887

Following gene: 75909889

Centisome position: 72.1

GC content: 40.61

Gene sequence:

>1251_bases
GTGAATATCAATTCCCTACTGCAACCATTTCACCATTTCGGCGTTAACCTGGGACTTTCCCGGATTATCAAATTGCTGGA
CAACCTTGGCAATCCTCATGAGCGAGTTCCCATAATTCATGTTGCTGGTACAAATGGCAAAGGTTCTGTTTGTGCTTATT
TGTCTTCCGTGCTGACTGAGGCTGGTTATCGTACAGGGCGCTATACTTCGCCCCATTTAGTTGATTGGCCAGAGCGTATT
TGCCTGAATGAACAGCAAATTTCTCATAACGAATTGTGTCAATTACTTTTAAGAGTTCAAGCGGCTATTTGCCTTGATGA
TGAGTATCCGACTCTATTTGAAGTCATTACCGCCGCCGCTTGGTTATATTTTGCTCAGCAACAAGTTGATGTGGCGGTGG
TAGAAGTAGGATTGGGTGGACGGTTGGATGCTACTAATGTAATTGCTGATCCTTTGGTGACAGTGATAACTTCCATCAGT
CGGGAACATTGGCAACAATTGGGGCCGACTGTGGCTGATATTGCTGGGGAAAAAGCGGGGATTCTTAAGCCTGGATGTCC
GGCTGTGGTGGGGATGTTGCCACCGGATGCAGAAAAAGTGGTGCGATCGCGCGCTCAAGAATTACAATGTCCTTTAATTC
TGCCTCAACCTGCTCGTCAAATCTCTCCCCAACTAGCAGAATATCAAAGTATTCAAAATACAAAAACAATTACATATCCT
CTACCTTTACAAGGACAAATTCAACTCAATAATTCTGCTTTGGCACTAGCAGCTTTAGAAATTTTGCAACAAAAAGGCTG
GCAGATTTCTGAAGAAGAAATTATTAATGGCATGGCAAAAACTAAATGGCCAGGTCGAATGCAATGGATTAATTGGAAAA
ATCACAAATTACTAATTGATGGCGCTCATAATCCGGCATCTGCTCAAGTGTTACGAAACTATGTAGATACCCTAAATACC
CCATCAGTAACTTGGGTTATGGGAATGTTATCTACAAAAGACCACGCCGATATTTTTCAAGTTTTATTACGACCAAATGA
CCAATTATATTTAGTTCCAGTACCAGATAATAATTCAGCCGATACAGAGTATTTAGGGAAATTAGCTTGTGATATTTGTC
CAGAATTAAGTACTTATCAAACTTGTCCAGATTTAAAACTAGCCTTGGAGACTGCATTTAACTCAACAGATAATTTGGTA
GTTTTGTGCGGTTCTTTATACTTAATTGGTTACTTTTTAAAGAGCTTGTAA

Upstream 100 bases:

>100_bases
CTGTTCTCTTTACAGAGACAGTCGGTTTTAGTATGGGGATTAGAGAGATGAGTAAATAAATCAATAAAAACCTTATTCCC
AATTACCTATTACCCATTCC

Downstream 100 bases:

>100_bases
GTTGCTATTAGAGACTAACTCTTCTATGATTATCAGGTTATTTATATCTGTTTATTCATATCAGTCATTTTACTAGCTTT
TACAAGTACGCTTTAAAGAA

Product: folylpolyglutamate synthetase

Products: NA

Alternate protein names: Folylpoly-gamma-glutamate synthetase; FPGS; Tetrahydrofolate synthase; Tetrahydrofolylpolyglutamate synthase [H]

Number of amino acids: Translated: 416; Mature: 416

Protein sequence:

>416_residues
MNINSLLQPFHHFGVNLGLSRIIKLLDNLGNPHERVPIIHVAGTNGKGSVCAYLSSVLTEAGYRTGRYTSPHLVDWPERI
CLNEQQISHNELCQLLLRVQAAICLDDEYPTLFEVITAAAWLYFAQQQVDVAVVEVGLGGRLDATNVIADPLVTVITSIS
REHWQQLGPTVADIAGEKAGILKPGCPAVVGMLPPDAEKVVRSRAQELQCPLILPQPARQISPQLAEYQSIQNTKTITYP
LPLQGQIQLNNSALALAALEILQQKGWQISEEEIINGMAKTKWPGRMQWINWKNHKLLIDGAHNPASAQVLRNYVDTLNT
PSVTWVMGMLSTKDHADIFQVLLRPNDQLYLVPVPDNNSADTEYLGKLACDICPELSTYQTCPDLKLALETAFNSTDNLV
VLCGSLYLIGYFLKSL

Sequences:

>Translated_416_residues
MNINSLLQPFHHFGVNLGLSRIIKLLDNLGNPHERVPIIHVAGTNGKGSVCAYLSSVLTEAGYRTGRYTSPHLVDWPERI
CLNEQQISHNELCQLLLRVQAAICLDDEYPTLFEVITAAAWLYFAQQQVDVAVVEVGLGGRLDATNVIADPLVTVITSIS
REHWQQLGPTVADIAGEKAGILKPGCPAVVGMLPPDAEKVVRSRAQELQCPLILPQPARQISPQLAEYQSIQNTKTITYP
LPLQGQIQLNNSALALAALEILQQKGWQISEEEIINGMAKTKWPGRMQWINWKNHKLLIDGAHNPASAQVLRNYVDTLNT
PSVTWVMGMLSTKDHADIFQVLLRPNDQLYLVPVPDNNSADTEYLGKLACDICPELSTYQTCPDLKLALETAFNSTDNLV
VLCGSLYLIGYFLKSL
>Mature_416_residues
MNINSLLQPFHHFGVNLGLSRIIKLLDNLGNPHERVPIIHVAGTNGKGSVCAYLSSVLTEAGYRTGRYTSPHLVDWPERI
CLNEQQISHNELCQLLLRVQAAICLDDEYPTLFEVITAAAWLYFAQQQVDVAVVEVGLGGRLDATNVIADPLVTVITSIS
REHWQQLGPTVADIAGEKAGILKPGCPAVVGMLPPDAEKVVRSRAQELQCPLILPQPARQISPQLAEYQSIQNTKTITYP
LPLQGQIQLNNSALALAALEILQQKGWQISEEEIINGMAKTKWPGRMQWINWKNHKLLIDGAHNPASAQVLRNYVDTLNT
PSVTWVMGMLSTKDHADIFQVLLRPNDQLYLVPVPDNNSADTEYLGKLACDICPELSTYQTCPDLKLALETAFNSTDNLV
VLCGSLYLIGYFLKSL

Specific function: Conversion of folates to polyglutamate derivatives. It preferes 5,10-methylenetetrahydrofolate, rather than 10- formyltetrahydrofolate as folate substrate [H]

COG id: COG0285

COG function: function code H; Folylpolyglutamate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the folylpolyglutamate synthase family [H]

Homologues:

Organism=Homo sapiens, GI66932990, Length=307, Percent_Identity=34.8534201954397, Blast_Score=157, Evalue=3e-38,
Organism=Homo sapiens, GI66932984, Length=307, Percent_Identity=34.8534201954397, Blast_Score=156, Evalue=4e-38,
Organism=Escherichia coli, GI1788654, Length=416, Percent_Identity=31.4903846153846, Blast_Score=167, Evalue=1e-42,
Organism=Caenorhabditis elegans, GI17553150, Length=295, Percent_Identity=32.8813559322034, Blast_Score=147, Evalue=1e-35,
Organism=Caenorhabditis elegans, GI17553148, Length=295, Percent_Identity=32.8813559322034, Blast_Score=147, Evalue=1e-35,
Organism=Caenorhabditis elegans, GI71984923, Length=295, Percent_Identity=32.8813559322034, Blast_Score=146, Evalue=2e-35,
Organism=Saccharomyces cerevisiae, GI6323760, Length=428, Percent_Identity=35.0467289719626, Blast_Score=207, Evalue=2e-54,
Organism=Saccharomyces cerevisiae, GI6324815, Length=302, Percent_Identity=33.112582781457, Blast_Score=139, Evalue=7e-34,
Organism=Saccharomyces cerevisiae, GI6322718, Length=437, Percent_Identity=25.858123569794, Blast_Score=100, Evalue=3e-22,
Organism=Drosophila melanogaster, GI24641571, Length=443, Percent_Identity=29.1196388261851, Blast_Score=159, Evalue=3e-39,
Organism=Drosophila melanogaster, GI24581568, Length=190, Percent_Identity=32.6315789473684, Blast_Score=104, Evalue=9e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018109
- InterPro:   IPR001645
- InterPro:   IPR004101
- InterPro:   IPR013221 [H]

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.17 [H]

Molecular weight: Translated: 45957; Mature: 45957

Theoretical pI: Translated: 5.57; Mature: 5.57

Prosite motif: PS01011 FOLYLPOLYGLU_SYNT_1 ; PS01012 FOLYLPOLYGLU_SYNT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNINSLLQPFHHFGVNLGLSRIIKLLDNLGNPHERVPIIHVAGTNGKGSVCAYLSSVLTE
CCHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHH
AGYRTGRYTSPHLVDWPERICLNEQQISHNELCQLLLRVQAAICLDDEYPTLFEVITAAA
CCCCCCCCCCCCCCCCHHHHHCCHHCCCHHHHHHHHHHHHHHHEECCCCHHHHHHHHHHH
WLYFAQQQVDVAVVEVGLGGRLDATNVIADPLVTVITSISREHWQQLGPTVADIAGEKAG
HHHHHHHCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCC
ILKPGCPAVVGMLPPDAEKVVRSRAQELQCPLILPQPARQISPQLAEYQSIQNTKTITYP
CCCCCCCCEECCCCCCHHHHHHHHHHHCCCCEECCCCHHHHCHHHHHHHHHCCCCEEEEC
LPLQGQIQLNNSALALAALEILQQKGWQISEEEIINGMAKTKWPGRMQWINWKNHKLLID
CCCCCEEEECCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCEEEEEECCCEEEEE
GAHNPASAQVLRNYVDTLNTPSVTWVMGMLSTKDHADIFQVLLRPNDQLYLVPVPDNNSA
CCCCCHHHHHHHHHHHHCCCCCHHHHHHHHCCCCHHHHHHHHHCCCCCEEEEECCCCCCC
DTEYLGKLACDICPELSTYQTCPDLKLALETAFNSTDNLVVLCGSLYLIGYFLKSL
CHHHHHHHHHHHCCCCHHHCCCCCHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHC
>Mature Secondary Structure
MNINSLLQPFHHFGVNLGLSRIIKLLDNLGNPHERVPIIHVAGTNGKGSVCAYLSSVLTE
CCHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHH
AGYRTGRYTSPHLVDWPERICLNEQQISHNELCQLLLRVQAAICLDDEYPTLFEVITAAA
CCCCCCCCCCCCCCCCHHHHHCCHHCCCHHHHHHHHHHHHHHHEECCCCHHHHHHHHHHH
WLYFAQQQVDVAVVEVGLGGRLDATNVIADPLVTVITSISREHWQQLGPTVADIAGEKAG
HHHHHHHCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCC
ILKPGCPAVVGMLPPDAEKVVRSRAQELQCPLILPQPARQISPQLAEYQSIQNTKTITYP
CCCCCCCCEECCCCCCHHHHHHHHHHHCCCCEECCCCHHHHCHHHHHHHHHCCCCEEEEC
LPLQGQIQLNNSALALAALEILQQKGWQISEEEIINGMAKTKWPGRMQWINWKNHKLLID
CCCCCEEEECCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCEEEEEECCCEEEEE
GAHNPASAQVLRNYVDTLNTPSVTWVMGMLSTKDHADIFQVLLRPNDQLYLVPVPDNNSA
CCCCCHHHHHHHHHHHHCCCCCHHHHHHHHCCCCHHHHHHHHHCCCCCEEEEECCCCCCC
DTEYLGKLACDICPELSTYQTCPDLKLALETAFNSTDNLVVLCGSLYLIGYFLKSL
CHHHHHHHHHHHCCCCHHHCCCCCHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8419299; 9384377; 2553669 [H]