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The map label for this gene is purB [H]

Identifier: 75909616

GI number: 75909616

Start: 4235012

End: 4236307

Strand: Direct

Name: purB [H]

Synonym: Ava_3410

Alternate gene names: 75909616

Gene position: 4235012-4236307 (Clockwise)

Preceding gene: 75909615

Following gene: 75909619

Centisome position: 66.53

GC content: 47.22

Gene sequence:

>1296_bases
TTGATAGAGCGTTATACTCTGCCCGAAATGGGCAACCTGTGGACTGACTTTTATAAACTAAAAACCTGGCTTCAGGTGGA
AATTGCAGTCTGTGAAGCCCAAGCGGAATTGGGTTATATTCCATCTGCTGCTGTTGAGGAAATCAAAGCTAAGGCAAATT
TTGATCCGCAACGGGTACTGGAAATAGAGGCGGAAGTCCGTCACGATGTCATCGCCTTTTTGACTAATGTCAATGAGTAT
GTGGGTGATGCTGGACGTTACATTCACTTGGGCTTAACTAGCTCTGATGTATTAGATACAGCTTTAGCTTTACAACTGGT
TGCTAGTTTGGATGTGTTGGCGCAACGCCTGGAAGGCTTGATTGGGGTGATTCGCCAAAAGGCACGGGAACACCGTTACA
CGGTAATGGCTGGCCGATCGCATGGTATTCACGCTGAACCAATTACTTTTGGGTTTAAATTGGCTGGTTGGTTGGCGGAA
GTGTTACGCCACCAACAACGGCTGACAATTTTGCGGGAAACGATCGCCGTGGGTAAGATTTCTGGCGCGGTGGGAACCTA
CGCTAATATTGAACCACGGGTGGAGGCGATCGCTTGTCAAAAACTCGGACTCAAACCCGATACAGCTTCTACACAAGTCA
TTTCCCGCGATATTCATGCTGATTTTGTGCAGCAGTTAGCCTTGATTGCTGCCTCTATTGAACGCTTTGCTGTAGAAATT
CGCAATCTACAAAAAACAGACGTTCTGGAAGTAGAGGAATTTTTCTCGAAGGGACAAAAAGGCTCTAGTGCGATGCCACA
TAAACGCAACCCCATCCGTTCGGAACGGCTGACGGGGATGGCGCGGCTAGTGAGGAGTCATGCCGGCGCAGTGTTAGAGG
ATGTTGCCCTGTGGCACGAACGGGACATTTCCCATAGTTCAGTGGAACGAGTAGTTCTGCCAGATGCCTGCATTTTGACC
CACTTTATGCTGATAGAAATCACCAATTTGGTGGAAAATCTGTTAGTCTACCCAGAAAATATGGCGCGGAATCTCAACTG
CTATGGTGGCGTTGTGTTCAGCCAAAAAGTTTTGCTGGCATTGGTAGATAAAGGACTCAGCCGAGAAGAAGCTTATGCGA
TCGTTCAGCAAAATGCTCATACTGCCTGGAATAAGCCGGAAGGCAATTTCCATGACTTGATCATTAACGACTCCCGTGTC
ACTCAACATTTGTCTCCAGCAGAAATTGCTGTGTGTTTTGACCCACAGCAACATCTCAAGCATTTGGATGAGGTTTATCG
CCGGTTGGATATTTAG

Upstream 100 bases:

>100_bases
AGTAACAATTGTTAACTGATGACTGTCAACTGACAACTATCAACTGACAACTATCAACCAAAGCGGGGTAAAATATGACA
GTTGTATTCTGCCGCGAACG

Downstream 100 bases:

>100_bases
GAATGGGGATTGGGTACAGGGAAAAAGGGGAAGAAATCTTTCTCTAAGTTCCTCTCCCTGCTCCCTACTTCTTAAATACG
CGCGAGTTTGATGGATTCGG

Product: adenylosuccinate lyase

Products: NA

Alternate protein names: ASL; Adenylosuccinase; ASase [H]

Number of amino acids: Translated: 431; Mature: 431

Protein sequence:

>431_residues
MIERYTLPEMGNLWTDFYKLKTWLQVEIAVCEAQAELGYIPSAAVEEIKAKANFDPQRVLEIEAEVRHDVIAFLTNVNEY
VGDAGRYIHLGLTSSDVLDTALALQLVASLDVLAQRLEGLIGVIRQKAREHRYTVMAGRSHGIHAEPITFGFKLAGWLAE
VLRHQQRLTILRETIAVGKISGAVGTYANIEPRVEAIACQKLGLKPDTASTQVISRDIHADFVQQLALIAASIERFAVEI
RNLQKTDVLEVEEFFSKGQKGSSAMPHKRNPIRSERLTGMARLVRSHAGAVLEDVALWHERDISHSSVERVVLPDACILT
HFMLIEITNLVENLLVYPENMARNLNCYGGVVFSQKVLLALVDKGLSREEAYAIVQQNAHTAWNKPEGNFHDLIINDSRV
TQHLSPAEIAVCFDPQQHLKHLDEVYRRLDI

Sequences:

>Translated_431_residues
MIERYTLPEMGNLWTDFYKLKTWLQVEIAVCEAQAELGYIPSAAVEEIKAKANFDPQRVLEIEAEVRHDVIAFLTNVNEY
VGDAGRYIHLGLTSSDVLDTALALQLVASLDVLAQRLEGLIGVIRQKAREHRYTVMAGRSHGIHAEPITFGFKLAGWLAE
VLRHQQRLTILRETIAVGKISGAVGTYANIEPRVEAIACQKLGLKPDTASTQVISRDIHADFVQQLALIAASIERFAVEI
RNLQKTDVLEVEEFFSKGQKGSSAMPHKRNPIRSERLTGMARLVRSHAGAVLEDVALWHERDISHSSVERVVLPDACILT
HFMLIEITNLVENLLVYPENMARNLNCYGGVVFSQKVLLALVDKGLSREEAYAIVQQNAHTAWNKPEGNFHDLIINDSRV
TQHLSPAEIAVCFDPQQHLKHLDEVYRRLDI
>Mature_431_residues
MIERYTLPEMGNLWTDFYKLKTWLQVEIAVCEAQAELGYIPSAAVEEIKAKANFDPQRVLEIEAEVRHDVIAFLTNVNEY
VGDAGRYIHLGLTSSDVLDTALALQLVASLDVLAQRLEGLIGVIRQKAREHRYTVMAGRSHGIHAEPITFGFKLAGWLAE
VLRHQQRLTILRETIAVGKISGAVGTYANIEPRVEAIACQKLGLKPDTASTQVISRDIHADFVQQLALIAASIERFAVEI
RNLQKTDVLEVEEFFSKGQKGSSAMPHKRNPIRSERLTGMARLVRSHAGAVLEDVALWHERDISHSSVERVVLPDACILT
HFMLIEITNLVENLLVYPENMARNLNCYGGVVFSQKVLLALVDKGLSREEAYAIVQQNAHTAWNKPEGNFHDLIINDSRV
TQHLSPAEIAVCFDPQQHLKHLDEVYRRLDI

Specific function: De novo purine biosynthesis; eighth step. [C]

COG id: COG0015

COG function: function code F; Adenylosuccinate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lyase 1 family. Adenylosuccinate lyase subfamily [H]

Homologues:

Organism=Homo sapiens, GI4557269, Length=422, Percent_Identity=29.6208530805687, Blast_Score=164, Evalue=2e-40,
Organism=Homo sapiens, GI183227688, Length=397, Percent_Identity=29.7229219143577, Blast_Score=158, Evalue=1e-38,
Organism=Escherichia coli, GI1787376, Length=329, Percent_Identity=28.2674772036474, Blast_Score=77, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17508577, Length=380, Percent_Identity=29.4736842105263, Blast_Score=138, Evalue=7e-33,
Organism=Caenorhabditis elegans, GI32564234, Length=347, Percent_Identity=30.2593659942363, Blast_Score=116, Evalue=2e-26,
Organism=Saccharomyces cerevisiae, GI6323391, Length=414, Percent_Identity=28.9855072463768, Blast_Score=156, Evalue=6e-39,
Organism=Drosophila melanogaster, GI24647570, Length=420, Percent_Identity=27.6190476190476, Blast_Score=134, Evalue=1e-31,
Organism=Drosophila melanogaster, GI221473854, Length=220, Percent_Identity=27.2727272727273, Blast_Score=65, Evalue=9e-11,
Organism=Drosophila melanogaster, GI78706858, Length=220, Percent_Identity=27.2727272727273, Blast_Score=65, Evalue=9e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019468
- InterPro:   IPR003031
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761
- InterPro:   IPR004769 [H]

Pfam domain/function: PF10397 ADSL_C; PF00206 Lyase_1 [H]

EC number: =4.3.2.2 [H]

Molecular weight: Translated: 48343; Mature: 48343

Theoretical pI: Translated: 6.37; Mature: 6.37

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIERYTLPEMGNLWTDFYKLKTWLQVEIAVCEAQAELGYIPSAAVEEIKAKANFDPQRVL
CCCCCCCCCHHHHHHHHHHHHHHHHEEEHEEHHHHHCCCCCHHHHHHHHHHCCCCHHHHH
EIEAEVRHDVIAFLTNVNEYVGDAGRYIHLGLTSSDVLDTALALQLVASLDVLAQRLEGL
HHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHH
IGVIRQKAREHRYTVMAGRSHGIHAEPITFGFKLAGWLAEVLRHQQRLTILRETIAVGKI
HHHHHHHHHHCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SGAVGTYANIEPRVEAIACQKLGLKPDTASTQVISRDIHADFVQQLALIAASIERFAVEI
CCCCCCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RNLQKTDVLEVEEFFSKGQKGSSAMPHKRNPIRSERLTGMARLVRSHAGAVLEDVALWHE
HCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RDISHSSVERVVLPDACILTHFMLIEITNLVENLLVYPENMARNLNCYGGVVFSQKVLLA
CCCCHHHHCEEECCCHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCHHCCHHHHHHHHHH
LVDKGLSREEAYAIVQQNAHTAWNKPEGNFHDLIINDSRVTQHLSPAEIAVCFDPQQHLK
HHHCCCCHHHHHHHHHCCCCCCCCCCCCCEEEEEEECHHHHHCCCCCCEEEEECHHHHHH
HLDEVYRRLDI
HHHHHHHHHCC
>Mature Secondary Structure
MIERYTLPEMGNLWTDFYKLKTWLQVEIAVCEAQAELGYIPSAAVEEIKAKANFDPQRVL
CCCCCCCCCHHHHHHHHHHHHHHHHEEEHEEHHHHHCCCCCHHHHHHHHHHCCCCHHHHH
EIEAEVRHDVIAFLTNVNEYVGDAGRYIHLGLTSSDVLDTALALQLVASLDVLAQRLEGL
HHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHH
IGVIRQKAREHRYTVMAGRSHGIHAEPITFGFKLAGWLAEVLRHQQRLTILRETIAVGKI
HHHHHHHHHHCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SGAVGTYANIEPRVEAIACQKLGLKPDTASTQVISRDIHADFVQQLALIAASIERFAVEI
CCCCCCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RNLQKTDVLEVEEFFSKGQKGSSAMPHKRNPIRSERLTGMARLVRSHAGAVLEDVALWHE
HCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RDISHSSVERVVLPDACILTHFMLIEITNLVENLLVYPENMARNLNCYGGVVFSQKVLLA
CCCCHHHHCEEECCCHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCHHCCHHHHHHHHHH
LVDKGLSREEAYAIVQQNAHTAWNKPEGNFHDLIINDSRVTQHLSPAEIAVCFDPQQHLK
HHHCCCCHHHHHHHHHCCCCCCCCCCCCCEEEEEEECHHHHHCCCCCCEEEEECHHHHHH
HLDEVYRRLDI
HHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]