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The map label for this gene is fabI [H]

Identifier: 75909491

GI number: 75909491

Start: 4097487

End: 4098281

Strand: Direct

Name: fabI [H]

Synonym: Ava_3284

Alternate gene names: 75909491

Gene position: 4097487-4098281 (Clockwise)

Preceding gene: 75909487

Following gene: 161567475

Centisome position: 64.37

GC content: 44.4

Gene sequence:

>795_bases
ATGACAACAAAAATCTCTATGCTGAATCTGACTGGAAAAAATGCCTTAGTTACAGGTATCGCCAATAATCGTTCCATTGC
CTGGGGGATTGCCCAACAACTGCACGCAGCTGGAGCCAACCTTGGTATTACTTATCTACCAGATGAACGTGGCAAATTTG
AGAAGAAAGTTTCAGAACTCGTAGAACCCCTCAACCCCAGCTTATTCTTGCCTTGTAATGTTCAAAATGATGAACAAATT
CAGTCTACTTTTGATACCATCCGCGATAAATGGGGCAGGTTAGATATTCTGATTCATTGTCTAGCCTTTGCTAACAGAGA
TGATTTGACTGGAGATTTTAGCCAAACATCCCGTGCTGGTTTTGCCACGGCTTTAGATATCAGCACTTTTTCACTGGTGC
AGTTAAGTGGTGCGGCTAAACCTCTGATGACCGAAGGTGGTAGCATTATTACTTTGTCATATTTGGGTGGTGTCAGAGCT
GTTCCTAACTACAACGTTATGGGAGTTGCTAAAGCGGGATTGGAAGCTAGTGTACGTTATCTAGCATCTGAACTCGGCTC
TCAAAATATTCGCGTTAACGCCATTTCTGCTGGCCCCATCCGTACCTTGGCTTCTAGCGCCGTTGGTGGCATTTTAGATA
TGATTCACCATGTAGAACAAGTAGCGCCCTTACGTCGGACTGTGACTCAGCTAGAAGTTGGCAATACAGCCGCCTTCTTG
GCTAGTGATTTAGCTAGTGGTATTACTGGTCAAGTCCTGTACGTAGATGCAGGATACGAAATCATGGGGATGTAA

Upstream 100 bases:

>100_bases
CTTTCCAGTTAAACAGCAAGAAAATTCCCAACATAACTTGATTACCTCTTCTACTGGGTAGTGATTTCTGTCAAGCTGTA
CAAATGAATCGATCACATTA

Downstream 100 bases:

>100_bases
TAAAATCTGGGTACTGGGTATTAGGGATTGGGGATTAGGTATGAGGAACAAACAAGGTGGAAAAAGAATGATTTTTTCAC
CACTTTTTCCTTATATGCCC

Product: enoyl-(acyl carrier protein) reductase

Products: NA

Alternate protein names: NADH-dependent enoyl-ACP reductase [H]

Number of amino acids: Translated: 264; Mature: 263

Protein sequence:

>264_residues
MTTKISMLNLTGKNALVTGIANNRSIAWGIAQQLHAAGANLGITYLPDERGKFEKKVSELVEPLNPSLFLPCNVQNDEQI
QSTFDTIRDKWGRLDILIHCLAFANRDDLTGDFSQTSRAGFATALDISTFSLVQLSGAAKPLMTEGGSIITLSYLGGVRA
VPNYNVMGVAKAGLEASVRYLASELGSQNIRVNAISAGPIRTLASSAVGGILDMIHHVEQVAPLRRTVTQLEVGNTAAFL
ASDLASGITGQVLYVDAGYEIMGM

Sequences:

>Translated_264_residues
MTTKISMLNLTGKNALVTGIANNRSIAWGIAQQLHAAGANLGITYLPDERGKFEKKVSELVEPLNPSLFLPCNVQNDEQI
QSTFDTIRDKWGRLDILIHCLAFANRDDLTGDFSQTSRAGFATALDISTFSLVQLSGAAKPLMTEGGSIITLSYLGGVRA
VPNYNVMGVAKAGLEASVRYLASELGSQNIRVNAISAGPIRTLASSAVGGILDMIHHVEQVAPLRRTVTQLEVGNTAAFL
ASDLASGITGQVLYVDAGYEIMGM
>Mature_263_residues
TTKISMLNLTGKNALVTGIANNRSIAWGIAQQLHAAGANLGITYLPDERGKFEKKVSELVEPLNPSLFLPCNVQNDEQIQ
STFDTIRDKWGRLDILIHCLAFANRDDLTGDFSQTSRAGFATALDISTFSLVQLSGAAKPLMTEGGSIITLSYLGGVRAV
PNYNVMGVAKAGLEASVRYLASELGSQNIRVNAISAGPIRTLASSAVGGILDMIHHVEQVAPLRRTVTQLEVGNTAAFLA
SDLASGITGQVLYVDAGYEIMGM

Specific function: Fatty acid biosynthesis pathway; second reduction step. [C]

COG id: COG0623

COG function: function code I; Enoyl-[acyl-carrier-protein] reductase (NADH)

Gene ontology:

Cell location: Inner Membrane-Associated [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family. FabI subfamily [H]

Homologues:

Organism=Homo sapiens, GI10190704, Length=199, Percent_Identity=32.6633165829146, Blast_Score=80, Evalue=2e-15,
Organism=Homo sapiens, GI40254992, Length=202, Percent_Identity=27.2277227722772, Blast_Score=69, Evalue=4e-12,
Organism=Homo sapiens, GI31542939, Length=215, Percent_Identity=28.3720930232558, Blast_Score=69, Evalue=4e-12,
Organism=Escherichia coli, GI1787545, Length=256, Percent_Identity=47.65625, Blast_Score=247, Evalue=7e-67,
Organism=Escherichia coli, GI87082100, Length=261, Percent_Identity=29.5019157088123, Blast_Score=82, Evalue=4e-17,
Organism=Escherichia coli, GI1789378, Length=252, Percent_Identity=28.1746031746032, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI1787335, Length=258, Percent_Identity=31.7829457364341, Blast_Score=76, Evalue=2e-15,
Organism=Escherichia coli, GI1787905, Length=255, Percent_Identity=29.0196078431373, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI87082160, Length=262, Percent_Identity=27.0992366412214, Blast_Score=66, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17562906, Length=274, Percent_Identity=28.8321167883212, Blast_Score=72, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI193204405, Length=275, Percent_Identity=29.8181818181818, Blast_Score=70, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17562910, Length=285, Percent_Identity=29.1228070175439, Blast_Score=69, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI17531453, Length=267, Percent_Identity=29.9625468164794, Blast_Score=68, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI115534694, Length=113, Percent_Identity=37.1681415929204, Blast_Score=67, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17536025, Length=269, Percent_Identity=27.1375464684015, Blast_Score=65, Evalue=5e-11,
Organism=Saccharomyces cerevisiae, GI6324126, Length=259, Percent_Identity=28.1853281853282, Blast_Score=70, Evalue=3e-13,
Organism=Drosophila melanogaster, GI24644337, Length=120, Percent_Identity=38.3333333333333, Blast_Score=75, Evalue=5e-14,
Organism=Drosophila melanogaster, GI21357041, Length=203, Percent_Identity=29.064039408867, Blast_Score=74, Evalue=1e-13,
Organism=Drosophila melanogaster, GI28571526, Length=223, Percent_Identity=29.5964125560538, Blast_Score=72, Evalue=5e-13,
Organism=Drosophila melanogaster, GI24644339, Length=261, Percent_Identity=26.0536398467433, Blast_Score=69, Evalue=5e-12,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002198
- InterPro:   IPR014358
- InterPro:   IPR002347
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00106 adh_short [H]

EC number: =1.3.1.9 [H]

Molecular weight: Translated: 28084; Mature: 27952

Theoretical pI: Translated: 6.12; Mature: 6.12

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTKISMLNLTGKNALVTGIANNRSIAWGIAQQLHAAGANLGITYLPDERGKFEKKVSEL
CCCEEEEEEECCCCEEEEEECCCCEEHHHHHHHHHHCCCCCCEEECCCCCCHHHHHHHHH
VEPLNPSLFLPCNVQNDEQIQSTFDTIRDKWGRLDILIHCLAFANRDDLTGDFSQTSRAG
HHCCCCCEEEEECCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCHHHHCCCC
FATALDISTFSLVQLSGAAKPLMTEGGSIITLSYLGGVRAVPNYNVMGVAKAGLEASVRY
CEEEEECCCEEEEEECCCCCCHHCCCCCEEEEEECCCEECCCCCCEEEEECCCHHHHHHH
LASELGSQNIRVNAISAGPIRTLASSAVGGILDMIHHVEQVAPLRRTVTQLEVGNTAAFL
HHHHHCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHH
ASDLASGITGQVLYVDAGYEIMGM
HHHHHCCCCCEEEEEECCEEEECC
>Mature Secondary Structure 
TTKISMLNLTGKNALVTGIANNRSIAWGIAQQLHAAGANLGITYLPDERGKFEKKVSEL
CCEEEEEEECCCCEEEEEECCCCEEHHHHHHHHHHCCCCCCEEECCCCCCHHHHHHHHH
VEPLNPSLFLPCNVQNDEQIQSTFDTIRDKWGRLDILIHCLAFANRDDLTGDFSQTSRAG
HHCCCCCEEEEECCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCHHHHCCCC
FATALDISTFSLVQLSGAAKPLMTEGGSIITLSYLGGVRAVPNYNVMGVAKAGLEASVRY
CEEEEECCCEEEEEECCCCCCHHCCCCCEEEEEECCCEECCCCCCEEEEECCCHHHHHHH
LASELGSQNIRVNAISAGPIRTLASSAVGGILDMIHHVEQVAPLRRTVTQLEVGNTAAFL
HHHHHCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHH
ASDLASGITGQVLYVDAGYEIMGM
HHHHHCCCCCEEEEEECCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8391534; 11759840 [H]