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The map label for this gene is pnp

Identifier: 75909486

GI number: 75909486

Start: 4090876

End: 4093032

Strand: Direct

Name: pnp

Synonym: Ava_3279

Alternate gene names: 75909486

Gene position: 4090876-4093032 (Clockwise)

Preceding gene: 75909485

Following gene: 75909487

Centisome position: 64.26

GC content: 48.54

Gene sequence:

>2157_bases
ATGGCAGAATTTGAGAAGTCAATATCCTTCGATGGAAGGGATATTCGACTGAAAGTAGGCCTACTAGCGCCCCAAGCTGG
TGGGTCGGTTTTGATAGAATCAGGGGATACAGCTGTTTTAGTCACAGCTACGCGATCGGCAGGTAGAGAGGGCATTGATT
TCCTTCCCCTGACAGTAGATTATGAAGAAAGACTATATGCGGCTGGTAGAATCCCCGGTGGGATTATGCGCCGAGAAGGC
CGTCCCCCAGAAAAAACAATTCTCACCAGCCGTCTCATAGACCGTCCCCTGCGTCCTTTGTTCCCTTCATGGTTGCGGGA
TGACTTGCAAATTGTCGCCTTAACAATGTCGATGGATGAGCAAGTACCGCCCGACGTGTTAGCAGTGACAGGTGCTTCCA
TTGCCACTCTCATTGCCAAAATCCCCTTTAACGGGCCGATGGCAGCAGTGCGGGTAGGCTTAGTGGGCGATGATTTCATT
ATTAACCCCACTTATGCAGAAATCGAGGCGGGAGACTTGGATCTGGTAGTCGCAGGTTCACCTCACGGCGTGATTATGGT
GGAAGCAGGGGCTAATCAATTACCAGAGCGAGATATTATCGAGGCGATTGATTTTGGTTATGAGGCAGTGCGCGACTTAA
TCAAAGCGCAGCTAGACCTAGTAGCCGAACTCGGTCTGGAAATTGTCCAAGAAGCACCGCCAGAGGTAGACCAAACTCTG
GAAAATTATATCCGCGATCGCGCTAGCGACGAAATTAAGAAAATTCTGGCTCAATTTGAACTAACCAAACCCGAACGCGA
TGCCGCGTTGGATGTTGTTAAAGATAATATTGCCACAGCGATCGCCGAACTCCCAGAAGAAGACCCAATTCGTTTAGCTG
CAACCGCCAACAGCAAAGCCCTGGGCAACACTTTTAAAGACATCACTAAATATTTCATGCGCCGTCAAATCGTCGAAGAT
AACGTGCGTGTTGATGGTCGAAAACTCGATCAAGTGCGCCCGGTTTCTTCCCAAGTAGGTGTCCTACCAAAGCGCGTCCA
CGGTAGCGGATTATTTAACCGAGGACTAACTCAGGTATTATCAGCTTGTACTTTGGGTACTCCTGGTGATGCCCAAAACC
TTAATGATGACCTGCAAACAGACCAATCAAAACGCTACCTCCATCATTACAACTTCCCGCCTTTCTCCGTTGGAGAAACC
AAGCCCTTACGCGCCCCTGGTAGACGGGAAATTGGTCATGGTGCATTAGCAGAGAGAGCCATCTTACCTGTACTGCCACC
CAAAGAGCAATTCCCTTACGTGATTCGCGTCGTCTCGGAAGTGCTTTCCTCCAACGGTTCCACCTCAATGGGTTCAGTTT
GTGGTTCCACCTTGGCATTAATGGATGCTGGTGTCCCCATCCTCAAACCCGTGAGTGGTGCAGCAATGGGCTTAATTAAA
GAAGGTGATGAAGTCAGAGTCCTGACAGATATTCAGGGTATTGAAGACTTTTTAGGTGACATGGACTTTAAAGTCGCTGG
TACCGATGCAGGGATTACCGCCTTGCAAATGGACATGAAAATCTCTGGTTTGTCTCTGGAGGTTATTGCCCAAGCTATTC
ACCAAGCAAAAGATGCTAGGCTGCATATTTTGGACAAGATGCTCCAAACTATCGATACCCCACGCACTGAAACCTCACCT
TACGCTCCTCGCCTGTTGACTATCAAGATTGATCCAGACATGATTGGTCTAGTCATTGGCCCTGGTGGTAAGACAATTAA
GGGCATCACCGAAGAAACAGGTGCAAAAATCGACATTGAAGACGATGGCACAGTGACAATTTCTGCGGTTGATGAAAACA
AGGCGAAAAGAGCGCGTAATATTGTCCAAGGCATGACTCGCAAACTCAACGAAGGCGATGTCTATGCTGGACGTGTGACT
CGGATTATTCCCATTGGCGCTTTTGTGGAATTCCTCCCTGGAAAAGAAGGGATGATTCACATTTCCCAATTAGCTGATTA
CCGTGTCGGCAAAGTTGAAGATGAAGTGGCGGTTGGCGATGAAGTAATTGTCAAAGTGCGCGAAATTGACAATAAGGGAC
GGATTAATCTCACACGCTTGGGTATACACCCAGATCAAGCAGCTGCGGCGCGGGAAGCTGCGGCGGTGAATCGTTAA

Upstream 100 bases:

>100_bases
CTGATGTTGCTCTTTGTATGTAACAGAAGCCAGCTACAGGGCATAAATGTTAGGATTGCCACAGAGAGAGAATAGCGTGC
ATAGAAGGAAAAAAAGCTGA

Downstream 100 bases:

>100_bases
ATCGATTGGGGATTTTAGATATTATCTAAAATCCGCGATGCCTCTTTACGTAGGCATCGCCGTAAAGTTTTTCATTATTG
TGAATAATCCCCACTAGAGA

Product: polynucleotide phosphorylase/polyadenylase

Products: NA

Alternate protein names: Polynucleotide phosphorylase; PNPase

Number of amino acids: Translated: 718; Mature: 717

Protein sequence:

>718_residues
MAEFEKSISFDGRDIRLKVGLLAPQAGGSVLIESGDTAVLVTATRSAGREGIDFLPLTVDYEERLYAAGRIPGGIMRREG
RPPEKTILTSRLIDRPLRPLFPSWLRDDLQIVALTMSMDEQVPPDVLAVTGASIATLIAKIPFNGPMAAVRVGLVGDDFI
INPTYAEIEAGDLDLVVAGSPHGVIMVEAGANQLPERDIIEAIDFGYEAVRDLIKAQLDLVAELGLEIVQEAPPEVDQTL
ENYIRDRASDEIKKILAQFELTKPERDAALDVVKDNIATAIAELPEEDPIRLAATANSKALGNTFKDITKYFMRRQIVED
NVRVDGRKLDQVRPVSSQVGVLPKRVHGSGLFNRGLTQVLSACTLGTPGDAQNLNDDLQTDQSKRYLHHYNFPPFSVGET
KPLRAPGRREIGHGALAERAILPVLPPKEQFPYVIRVVSEVLSSNGSTSMGSVCGSTLALMDAGVPILKPVSGAAMGLIK
EGDEVRVLTDIQGIEDFLGDMDFKVAGTDAGITALQMDMKISGLSLEVIAQAIHQAKDARLHILDKMLQTIDTPRTETSP
YAPRLLTIKIDPDMIGLVIGPGGKTIKGITEETGAKIDIEDDGTVTISAVDENKAKRARNIVQGMTRKLNEGDVYAGRVT
RIIPIGAFVEFLPGKEGMIHISQLADYRVGKVEDEVAVGDEVIVKVREIDNKGRINLTRLGIHPDQAAAAREAAAVNR

Sequences:

>Translated_718_residues
MAEFEKSISFDGRDIRLKVGLLAPQAGGSVLIESGDTAVLVTATRSAGREGIDFLPLTVDYEERLYAAGRIPGGIMRREG
RPPEKTILTSRLIDRPLRPLFPSWLRDDLQIVALTMSMDEQVPPDVLAVTGASIATLIAKIPFNGPMAAVRVGLVGDDFI
INPTYAEIEAGDLDLVVAGSPHGVIMVEAGANQLPERDIIEAIDFGYEAVRDLIKAQLDLVAELGLEIVQEAPPEVDQTL
ENYIRDRASDEIKKILAQFELTKPERDAALDVVKDNIATAIAELPEEDPIRLAATANSKALGNTFKDITKYFMRRQIVED
NVRVDGRKLDQVRPVSSQVGVLPKRVHGSGLFNRGLTQVLSACTLGTPGDAQNLNDDLQTDQSKRYLHHYNFPPFSVGET
KPLRAPGRREIGHGALAERAILPVLPPKEQFPYVIRVVSEVLSSNGSTSMGSVCGSTLALMDAGVPILKPVSGAAMGLIK
EGDEVRVLTDIQGIEDFLGDMDFKVAGTDAGITALQMDMKISGLSLEVIAQAIHQAKDARLHILDKMLQTIDTPRTETSP
YAPRLLTIKIDPDMIGLVIGPGGKTIKGITEETGAKIDIEDDGTVTISAVDENKAKRARNIVQGMTRKLNEGDVYAGRVT
RIIPIGAFVEFLPGKEGMIHISQLADYRVGKVEDEVAVGDEVIVKVREIDNKGRINLTRLGIHPDQAAAAREAAAVNR
>Mature_717_residues
AEFEKSISFDGRDIRLKVGLLAPQAGGSVLIESGDTAVLVTATRSAGREGIDFLPLTVDYEERLYAAGRIPGGIMRREGR
PPEKTILTSRLIDRPLRPLFPSWLRDDLQIVALTMSMDEQVPPDVLAVTGASIATLIAKIPFNGPMAAVRVGLVGDDFII
NPTYAEIEAGDLDLVVAGSPHGVIMVEAGANQLPERDIIEAIDFGYEAVRDLIKAQLDLVAELGLEIVQEAPPEVDQTLE
NYIRDRASDEIKKILAQFELTKPERDAALDVVKDNIATAIAELPEEDPIRLAATANSKALGNTFKDITKYFMRRQIVEDN
VRVDGRKLDQVRPVSSQVGVLPKRVHGSGLFNRGLTQVLSACTLGTPGDAQNLNDDLQTDQSKRYLHHYNFPPFSVGETK
PLRAPGRREIGHGALAERAILPVLPPKEQFPYVIRVVSEVLSSNGSTSMGSVCGSTLALMDAGVPILKPVSGAAMGLIKE
GDEVRVLTDIQGIEDFLGDMDFKVAGTDAGITALQMDMKISGLSLEVIAQAIHQAKDARLHILDKMLQTIDTPRTETSPY
APRLLTIKIDPDMIGLVIGPGGKTIKGITEETGAKIDIEDDGTVTISAVDENKAKRARNIVQGMTRKLNEGDVYAGRVTR
IIPIGAFVEFLPGKEGMIHISQLADYRVGKVEDEVAVGDEVIVKVREIDNKGRINLTRLGIHPDQAAAAREAAAVNR

Specific function: Involved in mRNA degradation. Hydrolyzes single-stranded polyribonucleotides processively in the 3'- to 5'-direction

COG id: COG1185

COG function: function code J; Polyribonucleotide nucleotidyltransferase (polynucleotide phosphorylase)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain

Homologues:

Organism=Homo sapiens, GI188528628, Length=725, Percent_Identity=36.6896551724138, Blast_Score=436, Evalue=1e-122,
Organism=Escherichia coli, GI145693187, Length=696, Percent_Identity=48.4195402298851, Blast_Score=621, Evalue=1e-179,
Organism=Caenorhabditis elegans, GI115534063, Length=675, Percent_Identity=34.2222222222222, Blast_Score=358, Evalue=5e-99,
Organism=Drosophila melanogaster, GI281362905, Length=698, Percent_Identity=37.5358166189112, Blast_Score=434, Evalue=1e-122,
Organism=Drosophila melanogaster, GI24651641, Length=698, Percent_Identity=37.5358166189112, Blast_Score=434, Evalue=1e-122,
Organism=Drosophila melanogaster, GI24651643, Length=698, Percent_Identity=37.5358166189112, Blast_Score=434, Evalue=1e-122,
Organism=Drosophila melanogaster, GI161079377, Length=642, Percent_Identity=37.0716510903427, Blast_Score=399, Evalue=1e-111,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1000 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 3328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media

Swissprot (AC and ID): PNP_ANAVT (Q3M7Z9)

Other databases:

- EMBL:   CP000117
- RefSeq:   YP_323782.1
- HSSP:   Q91WJ8
- ProteinModelPortal:   Q3M7Z9
- STRING:   Q3M7Z9
- GeneID:   3680310
- GenomeReviews:   CP000117_GR
- KEGG:   ava:Ava_3279
- NMPDR:   fig|240292.3.peg.2302
- eggNOG:   COG1185
- HOGENOM:   HBG382411
- OMA:   YGETVVL
- PhylomeDB:   Q3M7Z9
- ProtClustDB:   PRK11824
- BioCyc:   AVAR240292:AVA_3279-MONOMER
- GO:   GO:0005739
- HAMAP:   MF_01595
- InterPro:   IPR001247
- InterPro:   IPR015847
- InterPro:   IPR004087
- InterPro:   IPR004088
- InterPro:   IPR018111
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR012162
- InterPro:   IPR015848
- InterPro:   IPR003029
- InterPro:   IPR020568
- InterPro:   IPR022967
- Gene3D:   G3DSA:2.40.50.140
- Gene3D:   G3DSA:1.10.10.400
- PANTHER:   PTHR11252
- PIRSF:   PIRSF005499
- SMART:   SM00322
- SMART:   SM00316
- TIGRFAMs:   TIGR03591

Pfam domain/function: PF00013 KH_1; PF03726 PNPase; PF01138 RNase_PH; PF03725 RNase_PH_C; PF00575 S1; SSF46915 3_ExoRNase; SSF55666 3_ExoRNase; SSF50249 Nucleic_acid_OB; SSF54211 Ribosomal_S5_D2-typ_fold

EC number: =2.7.7.8

Molecular weight: Translated: 77752; Mature: 77621

Theoretical pI: Translated: 4.81; Mature: 4.81

Prosite motif: PS50084 KH_TYPE_1; PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEFEKSISFDGRDIRLKVGLLAPQAGGSVLIESGDTAVLVTATRSAGREGIDFLPLTVD
CCCCCCCCCCCCCEEEEEEEEECCCCCCEEEEECCCEEEEEEEECCCCCCCCEEEEEEEC
YEERLYAAGRIPGGIMRREGRPPEKTILTSRLIDRPLRPLFPSWLRDDLQIVALTMSMDE
HHHHHHCCCCCCCHHHHCCCCCCHHHHHHHHHHHCCCHHCCHHHHHCCEEEEEEEECCCC
QVPPDVLAVTGASIATLIAKIPFNGPMAAVRVGLVGDDFIINPTYAEIEAGDLDLVVAGS
CCCCCCEEECCHHHHHHHHHCCCCCCHHHEEEEEECCCEEECCCCCEEECCCEEEEEECC
PHGVIMVEAGANQLPERDIIEAIDFGYEAVRDLIKAQLDLVAELGLEIVQEAPPEVDQTL
CCCEEEEECCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
ENYIRDRASDEIKKILAQFELTKPERDAALDVVKDNIATAIAELPEEDPIRLAATANSKA
HHHHHHCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCH
LGNTFKDITKYFMRRQIVEDNVRVDGRKLDQVRPVSSQVGVLPKRVHGSGLFNRGLTQVL
HHHHHHHHHHHHHHHHHHHCCCCCCCCHHCCCCCCHHHHCCCCHHHCCCCHHHHHHHHHH
SACTLGTPGDAQNLNDDLQTDQSKRYLHHYNFPPFSVGETKPLRAPGRREIGHGALAERA
HHHCCCCCCCCCCCCHHHCCCHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHCC
ILPVLPPKEQFPYVIRVVSEVLSSNGSTSMGSVCGSTLALMDAGVPILKPVSGAAMGLIK
CCCCCCCCHHCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHCC
EGDEVRVLTDIQGIEDFLGDMDFKVAGTDAGITALQMDMKISGLSLEVIAQAIHQAKDAR
CCCCEEEEEHHHHHHHHHCCCCEEEECCCCCCEEEEEECEECCCHHHHHHHHHHHHHHHH
LHILDKMLQTIDTPRTETSPYAPRLLTIKIDPDMIGLVIGPGGKTIKGITEETGAKIDIE
HHHHHHHHHHHCCCCCCCCCCCCEEEEEEECCCEEEEEECCCCCEECCCHHCCCCEEEEC
DDGTVTISAVDENKAKRARNIVQGMTRKLNEGDVYAGRVTRIIPIGAFVEFLPGKEGMIH
CCCEEEEEECCCHHHHHHHHHHHHHHHCCCCCCEEECCEEEEEEHHHHHHHCCCCCCCEE
ISQLADYRVGKVEDEVAVGDEVIVKVREIDNKGRINLTRLGIHPDQAAAAREAAAVNR
HHHHHCCCCCCCCCHHHCCHHHEEEEEECCCCCCEEEEEECCCCCHHHHHHHHHHCCC
>Mature Secondary Structure 
AEFEKSISFDGRDIRLKVGLLAPQAGGSVLIESGDTAVLVTATRSAGREGIDFLPLTVD
CCCCCCCCCCCCEEEEEEEEECCCCCCEEEEECCCEEEEEEEECCCCCCCCEEEEEEEC
YEERLYAAGRIPGGIMRREGRPPEKTILTSRLIDRPLRPLFPSWLRDDLQIVALTMSMDE
HHHHHHCCCCCCCHHHHCCCCCCHHHHHHHHHHHCCCHHCCHHHHHCCEEEEEEEECCCC
QVPPDVLAVTGASIATLIAKIPFNGPMAAVRVGLVGDDFIINPTYAEIEAGDLDLVVAGS
CCCCCCEEECCHHHHHHHHHCCCCCCHHHEEEEEECCCEEECCCCCEEECCCEEEEEECC
PHGVIMVEAGANQLPERDIIEAIDFGYEAVRDLIKAQLDLVAELGLEIVQEAPPEVDQTL
CCCEEEEECCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
ENYIRDRASDEIKKILAQFELTKPERDAALDVVKDNIATAIAELPEEDPIRLAATANSKA
HHHHHHCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCH
LGNTFKDITKYFMRRQIVEDNVRVDGRKLDQVRPVSSQVGVLPKRVHGSGLFNRGLTQVL
HHHHHHHHHHHHHHHHHHHCCCCCCCCHHCCCCCCHHHHCCCCHHHCCCCHHHHHHHHHH
SACTLGTPGDAQNLNDDLQTDQSKRYLHHYNFPPFSVGETKPLRAPGRREIGHGALAERA
HHHCCCCCCCCCCCCHHHCCCHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHCC
ILPVLPPKEQFPYVIRVVSEVLSSNGSTSMGSVCGSTLALMDAGVPILKPVSGAAMGLIK
CCCCCCCCHHCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHCC
EGDEVRVLTDIQGIEDFLGDMDFKVAGTDAGITALQMDMKISGLSLEVIAQAIHQAKDAR
CCCCEEEEEHHHHHHHHHCCCCEEEECCCCCCEEEEEECEECCCHHHHHHHHHHHHHHHH
LHILDKMLQTIDTPRTETSPYAPRLLTIKIDPDMIGLVIGPGGKTIKGITEETGAKIDIE
HHHHHHHHHHHCCCCCCCCCCCCEEEEEEECCCEEEEEECCCCCEECCCHHCCCCEEEEC
DDGTVTISAVDENKAKRARNIVQGMTRKLNEGDVYAGRVTRIIPIGAFVEFLPGKEGMIH
CCCEEEEEECCCHHHHHHHHHHHHHHHCCCCCCEEECCEEEEEEHHHHHHHCCCCCCCEE
ISQLADYRVGKVEDEVAVGDEVIVKVREIDNKGRINLTRLGIHPDQAAAAREAAAVNR
HHHHHCCCCCCCCCHHHCCHHHEEEEEECCCCCCEEEEEECCCCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA