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The map label for this gene is ytcB [H]

Identifier: 75909435

GI number: 75909435

Start: 4014268

End: 4015218

Strand: Direct

Name: ytcB [H]

Synonym: Ava_3228

Alternate gene names: 75909435

Gene position: 4014268-4015218 (Clockwise)

Preceding gene: 75909429

Following gene: 75909439

Centisome position: 63.06

GC content: 43.74

Gene sequence:

>951_bases
ATGGCAAAAATTATAGTTACTGGAGCAGCAGGATTTATTGGTTCGCACCTGGTAGAAACCCTCCTAAAACAAGGTGAAGA
AGTAATCGGGATTGATGAAGTGAATGATTACTACGATCCCTTGTTAAAACGTAAAAATATCGCCTATTTACAATCGTCGC
CTAACTTCACTTTCATTGAAGGAGATATTCAGTTTCTAGATTGGCCGTCTCTCCTCCAAGATGTGACTGTGGTCTACCAT
CAGGCGGCGCAGGCGGGAGTTAGGGCAAGTTGGGGTAAAGGTTTCCGTGCTTATACTGAGCGCAATATTAATGCAACACA
AGTTTTGTTGGAAGCAGCTAAGGATGCTCAACAATTAACTAGGTTTGTGTTTGCATCTACTTCTAGTGTGTATGGGGATG
CGGAAACCTTACCTACTCACGAAGGAATTCCTCCTCAACCGGTTTCTCCTTATGGAATTACTAAGTTAGCCGCCGAGCGT
TTGTGTGGACTGTATCACAAAAATTTCGGTGTGCCGTTTGTGGCTTTACGTTATTTCACTGTATATGGCCCTAGACAGCG
CCCTGATATGGCTTTCCATAAGTTTTTTAAATCGGTTTTACAGGATGAAGCGATTCCTGTTTACGGAGATGGACAGCAAA
CTCGTGACTTTACATTTGTGAGCGATGCTGTAGCGGCCAATTTAGCGGCTGCTAGTGTACCAACTGCTGTAGGAGAAATT
TTTAATATTGGTGGTGGTAGTAGAGTGGTTTTGGCTGAGGTCTTAGACACAATGGAACAAATTGTTGGTCAGCCAATTAA
GAGAAATCACATAGAAAAGGCAATGGGTGATGCACGTCACACGGCTGCTGATGTATCTAAGGCACGGAAAATTTTAGGAT
ATGAACCACAGGTTTCTTTGAGGGAGGGTTTGAGCCTAGAATGGCAGTGGGTGAAGGCTTTGTACGCCTAG

Upstream 100 bases:

>100_bases
TGATACGGTGGGCATTGCCCACCCTACAAATACTTAGATTTGTTCAATAATCAAATCGGATTCCTATAGAAACTAAGAAA
ATAAGAATAAAGTAGGTAAC

Downstream 100 bases:

>100_bases
AGCCAGTGAAAGCGATCGCTTGGATTAAACTAAGAGGGTTTAGCTAAAGCGATCGCACTAATTCCACTCAACAGCAAACC
TATGCCTATCCATTGGGAAA

Product: 3-beta hydroxysteroid dehydrogenase/isomerase

Products: dTDP-4-dehydro-6-deoxy-D-glucose; H2O [C]

Alternate protein names: NA

Number of amino acids: Translated: 316; Mature: 315

Protein sequence:

>316_residues
MAKIIVTGAAGFIGSHLVETLLKQGEEVIGIDEVNDYYDPLLKRKNIAYLQSSPNFTFIEGDIQFLDWPSLLQDVTVVYH
QAAQAGVRASWGKGFRAYTERNINATQVLLEAAKDAQQLTRFVFASTSSVYGDAETLPTHEGIPPQPVSPYGITKLAAER
LCGLYHKNFGVPFVALRYFTVYGPRQRPDMAFHKFFKSVLQDEAIPVYGDGQQTRDFTFVSDAVAANLAAASVPTAVGEI
FNIGGGSRVVLAEVLDTMEQIVGQPIKRNHIEKAMGDARHTAADVSKARKILGYEPQVSLREGLSLEWQWVKALYA

Sequences:

>Translated_316_residues
MAKIIVTGAAGFIGSHLVETLLKQGEEVIGIDEVNDYYDPLLKRKNIAYLQSSPNFTFIEGDIQFLDWPSLLQDVTVVYH
QAAQAGVRASWGKGFRAYTERNINATQVLLEAAKDAQQLTRFVFASTSSVYGDAETLPTHEGIPPQPVSPYGITKLAAER
LCGLYHKNFGVPFVALRYFTVYGPRQRPDMAFHKFFKSVLQDEAIPVYGDGQQTRDFTFVSDAVAANLAAASVPTAVGEI
FNIGGGSRVVLAEVLDTMEQIVGQPIKRNHIEKAMGDARHTAADVSKARKILGYEPQVSLREGLSLEWQWVKALYA
>Mature_315_residues
AKIIVTGAAGFIGSHLVETLLKQGEEVIGIDEVNDYYDPLLKRKNIAYLQSSPNFTFIEGDIQFLDWPSLLQDVTVVYHQ
AAQAGVRASWGKGFRAYTERNINATQVLLEAAKDAQQLTRFVFASTSSVYGDAETLPTHEGIPPQPVSPYGITKLAAERL
CGLYHKNFGVPFVALRYFTVYGPRQRPDMAFHKFFKSVLQDEAIPVYGDGQQTRDFTFVSDAVAANLAAASVPTAVGEIF
NIGGGSRVVLAEVLDTMEQIVGQPIKRNHIEKAMGDARHTAADVSKARKILGYEPQVSLREGLSLEWQWVKALYA

Specific function: DTDP-L-RHAMNOSE BIOSYNTHESIS WITHIN THE O ANTIGEN BIOSYNTHESIS PATHWAY OF LIPOPOLYSACCHARIDE BIOSYNTHESIS. [C]

COG id: COG0451

COG function: function code MG; Nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family [H]

Homologues:

Organism=Homo sapiens, GI7657641, Length=323, Percent_Identity=30.9597523219814, Blast_Score=144, Evalue=7e-35,
Organism=Homo sapiens, GI42516563, Length=310, Percent_Identity=30.6451612903226, Blast_Score=129, Evalue=4e-30,
Organism=Homo sapiens, GI56237023, Length=343, Percent_Identity=27.4052478134111, Blast_Score=82, Evalue=7e-16,
Organism=Homo sapiens, GI56118217, Length=343, Percent_Identity=27.4052478134111, Blast_Score=82, Evalue=7e-16,
Organism=Homo sapiens, GI189083684, Length=343, Percent_Identity=27.4052478134111, Blast_Score=82, Evalue=7e-16,
Organism=Escherichia coli, GI1788353, Length=350, Percent_Identity=29.7142857142857, Blast_Score=122, Evalue=4e-29,
Organism=Escherichia coli, GI48994969, Length=341, Percent_Identity=30.2052785923754, Blast_Score=121, Evalue=5e-29,
Organism=Escherichia coli, GI1786974, Length=336, Percent_Identity=27.9761904761905, Blast_Score=104, Evalue=9e-24,
Organism=Escherichia coli, GI1790049, Length=262, Percent_Identity=27.0992366412214, Blast_Score=68, Evalue=6e-13,
Organism=Caenorhabditis elegans, GI17568069, Length=321, Percent_Identity=28.6604361370716, Blast_Score=116, Evalue=1e-26,
Organism=Caenorhabditis elegans, GI17539532, Length=315, Percent_Identity=28.8888888888889, Blast_Score=109, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI115532424, Length=322, Percent_Identity=25.4658385093168, Blast_Score=94, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI71982035, Length=347, Percent_Identity=27.3775216138329, Blast_Score=86, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI71982038, Length=349, Percent_Identity=27.5071633237822, Blast_Score=85, Evalue=5e-17,
Organism=Saccharomyces cerevisiae, GI6319493, Length=338, Percent_Identity=26.9230769230769, Blast_Score=87, Evalue=4e-18,
Organism=Drosophila melanogaster, GI21356223, Length=312, Percent_Identity=30.7692307692308, Blast_Score=123, Evalue=2e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: 4.2.1.46 [C]

Molecular weight: Translated: 34829; Mature: 34698

Theoretical pI: Translated: 6.41; Mature: 6.41

Prosite motif: PS00061 ADH_SHORT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKIIVTGAAGFIGSHLVETLLKQGEEVIGIDEVNDYYDPLLKRKNIAYLQSSPNFTFIE
CCEEEEECCHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHCCCCEEEECCCCEEEEE
GDIQFLDWPSLLQDVTVVYHQAAQAGVRASWGKGFRAYTERNINATQVLLEAAKDAQQLT
CCEEECCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHH
RFVFASTSSVYGDAETLPTHEGIPPQPVSPYGITKLAAERLCGLYHKNFGVPFVALRYFT
HHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
VYGPRQRPDMAFHKFFKSVLQDEAIPVYGDGQQTRDFTFVSDAVAANLAAASVPTAVGEI
HCCCCCCCCHHHHHHHHHHHHCCCCCEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
FNIGGGSRVVLAEVLDTMEQIVGQPIKRNHIEKAMGDARHTAADVSKARKILGYEPQVSL
HCCCCCCEEHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHH
REGLSLEWQWVKALYA
HCCCCCHHHHHHHHCC
>Mature Secondary Structure 
AKIIVTGAAGFIGSHLVETLLKQGEEVIGIDEVNDYYDPLLKRKNIAYLQSSPNFTFIE
CEEEEECCHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHCCCCEEEECCCCEEEEE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HCCCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NAD+ [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.019 {NAD+}} 0.034 {dTDPglucose}} [C]

Substrates: dTDPglucose [C]

Specific reaction: dTDPglucose --> dTDP-4-dehydro-6-deoxy-D-glucose + H2O [C]

General reaction: Elimination (of H2O C-O bond cleavage [C]

Inhibitor: TDP; TTP [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9387221; 9384377 [H]