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The map label for this gene is thiG

Identifier: 75909426

GI number: 75909426

Start: 4001598

End: 4003556

Strand: Direct

Name: thiG

Synonym: Ava_3219

Alternate gene names: 75909426

Gene position: 4001598-4003556 (Clockwise)

Preceding gene: 75909425

Following gene: 75909427

Centisome position: 62.86

GC content: 48.85

Gene sequence:

>1959_bases
ATGACTAGGGACATTGTAATTATTGGTGGCGGCGTTATTGGTTTGGCGATCGCCGTTGAACTTAAGTTGCGCGGGACAAA
AGTTACCGTGCTTTGTCGTGATTTCCCAGCAGCAGCAGCTCACGCCGCCGCCGGGATGTTAGCCCCGGATGCCGAGGAAA
TCACAGATGAGGCGATGAAGTCGCTATGCTGGCGATCGCGTTCTTTATATCCCGAATGGACAAGCAAGTTAGAAGATTTA
ACGGGTTTAAACACTGGTTACTGGCCTTGTGGCATCCTAGCGCCAGTTTATGAAGGGCAGGAGAGCAAGGGTGTAAGAAT
TCAGGAAAATAAGGGAGAATCACCCGCTTATTGGTTAGAAAAAGCCGTTATTCATCAATATCAACCAGGATTAGGTGAGG
ATGTGGTTGGTGGTTGGTGGTATCCAGAGGATGCCCAAGTGAATAATCAAGCACTAGCGCGTGTGCTGTGGGCGGCGGCG
GAAAGCCTTGGTGTGGAACTCAACGACGGAATTACAGTAGAAGGATTATTACAACAGCAGGGACAGGTAGTAGGTGTCCA
AACCAACACCGGCATCATTCAGGCCGAACACTATGTTTTAGCCACAGGTGCTTGGGCAAATGAATTATTACCCTTACCCG
TAACCCCTCGTAAAGGGCAAATGTTGCGTGTGCGTGTGCCGGAATCTGTACCGGAATTGCCTTTAAAGCGGGTTTTATTT
GGCGAAAATATTTACATTGTACCGAGACGAGACCGATCTATTATTATTGGGGCAACGAGTGAAGATGTCGGCTTTACCCC
CCACAACACCCCCGCCGGCATTCAAACTTTACTGCAAGGCGCAATTCGTCTCTATCCTCAGTTACAGGATTATCCCATTC
AAGAATTTTGGTGGGGCTTTCGTCCAGCCACTCCAGATGAATTACCCATTTTAGGAACTAGTCACTGTGCCAATTTAACC
TTGGCTACTGGTCATTATCGCAACGGTATCTTACTAGCACCAATAACCGCCGCACTTATAGCCGATTTCATCGTAGAACA
AAAATCTGACCCCCTACTGTCTCATTTCCACTACTCACGCTTTCAAAAACAGGCATCTACCACCCCCATGTTTACCCACT
CCGCCAACTTCTCCAACGGACACGCCAAAAACCCTCCACTCCCCACTCTAGACTCATCCCTCATCATTGCAGGCAAATCC
TTTCATTCCCGTTTGATGACGGGGACAGGCAAATATCGCAGCATAGAAGAAATGCAGCAAAGCGTTGTTGCTAGCGGTTG
CGAAATTGTCACGGTGGCGGTGCGACGAGTCCAAACCAAAGCCCCAGGCCATGAAGGTTTAGCCGAAGCCCTGGACTGGT
CGAGAATTTGGATGTTGCCGAATACAGCTGGCTGTCAAACCGCAGAAGAAGCCATTCGGGTAGCGCGTTTGGGGAGAGAA
ATGGCTAAGTTATTAGGTCAGGAAGATAATAATTTTGTCAAATTAGAAGTTATACCAGACCCCAAATATTTACTTCCCGA
CCCCATTGGTACATTACAAGCTGCCGAACAGTTAGTGAAAGAAGGTTTCGCCGTCTTACCTTATATCAACGCTGACCCCA
TGCTAGCCAAGCGGTTAGAAGATGTCGGTTGTGCTACAGTCATGCCTTTAGCGTCACCCATCGGCTCAGGACAGGGTTTA
AAAACCACCGCCAACATTCAAATTATCATCGAAAACGCCAAGATCCCAGTCGTGGTAGATGCTGGCATTGGTGCGCCCTC
AGAAGCCTCCCAGGCGATGGAATTAGGGGCAGATGCCCTATTAATTAATAGTGCGATCGCCCTTGCTCAAAACCCAGCCG
CAATGGCTCAAGCCATGAACCTCGCAACAGTTGCCGGTCGTCTAGCCTACCTCGCAGGTAGAATGCCCATTAAAACCTAT
GCCAGTGCTAGTTCACCAGTCACAGGTACGATTAGTTAG

Upstream 100 bases:

>100_bases
TCCCTAGTCCCTCACCACTTGTACTAGTCGTTGGTTACAAGTTATGAGTTATTAGTTATGTACTAATGGTAAATAGCTAA
TATATAAATACTAATGACTA

Downstream 100 bases:

>100_bases
TCAGTGGTCAATAGTCAGTAGTCAGTAGTCAGTAGTCCATAGTGATTGCTTTGGACTGTTGACCAATGAGTAACAACAAC
TGACAACTGACAACTAACCA

Product: thiazole synthase

Products: 4-methyl-5-(beta-hydroxyethyl)thiazole phosphate; 4-hydroxy-benzyl-alcohol; C1 of tyrosine; ThiS protein [C]

Alternate protein names: Probable FAD-dependent glycine oxidase; Thiazole synthase

Number of amino acids: Translated: 652; Mature: 651

Protein sequence:

>652_residues
MTRDIVIIGGGVIGLAIAVELKLRGTKVTVLCRDFPAAAAHAAAGMLAPDAEEITDEAMKSLCWRSRSLYPEWTSKLEDL
TGLNTGYWPCGILAPVYEGQESKGVRIQENKGESPAYWLEKAVIHQYQPGLGEDVVGGWWYPEDAQVNNQALARVLWAAA
ESLGVELNDGITVEGLLQQQGQVVGVQTNTGIIQAEHYVLATGAWANELLPLPVTPRKGQMLRVRVPESVPELPLKRVLF
GENIYIVPRRDRSIIIGATSEDVGFTPHNTPAGIQTLLQGAIRLYPQLQDYPIQEFWWGFRPATPDELPILGTSHCANLT
LATGHYRNGILLAPITAALIADFIVEQKSDPLLSHFHYSRFQKQASTTPMFTHSANFSNGHAKNPPLPTLDSSLIIAGKS
FHSRLMTGTGKYRSIEEMQQSVVASGCEIVTVAVRRVQTKAPGHEGLAEALDWSRIWMLPNTAGCQTAEEAIRVARLGRE
MAKLLGQEDNNFVKLEVIPDPKYLLPDPIGTLQAAEQLVKEGFAVLPYINADPMLAKRLEDVGCATVMPLASPIGSGQGL
KTTANIQIIIENAKIPVVVDAGIGAPSEASQAMELGADALLINSAIALAQNPAAMAQAMNLATVAGRLAYLAGRMPIKTY
ASASSPVTGTIS

Sequences:

>Translated_652_residues
MTRDIVIIGGGVIGLAIAVELKLRGTKVTVLCRDFPAAAAHAAAGMLAPDAEEITDEAMKSLCWRSRSLYPEWTSKLEDL
TGLNTGYWPCGILAPVYEGQESKGVRIQENKGESPAYWLEKAVIHQYQPGLGEDVVGGWWYPEDAQVNNQALARVLWAAA
ESLGVELNDGITVEGLLQQQGQVVGVQTNTGIIQAEHYVLATGAWANELLPLPVTPRKGQMLRVRVPESVPELPLKRVLF
GENIYIVPRRDRSIIIGATSEDVGFTPHNTPAGIQTLLQGAIRLYPQLQDYPIQEFWWGFRPATPDELPILGTSHCANLT
LATGHYRNGILLAPITAALIADFIVEQKSDPLLSHFHYSRFQKQASTTPMFTHSANFSNGHAKNPPLPTLDSSLIIAGKS
FHSRLMTGTGKYRSIEEMQQSVVASGCEIVTVAVRRVQTKAPGHEGLAEALDWSRIWMLPNTAGCQTAEEAIRVARLGRE
MAKLLGQEDNNFVKLEVIPDPKYLLPDPIGTLQAAEQLVKEGFAVLPYINADPMLAKRLEDVGCATVMPLASPIGSGQGL
KTTANIQIIIENAKIPVVVDAGIGAPSEASQAMELGADALLINSAIALAQNPAAMAQAMNLATVAGRLAYLAGRMPIKTY
ASASSPVTGTIS
>Mature_651_residues
TRDIVIIGGGVIGLAIAVELKLRGTKVTVLCRDFPAAAAHAAAGMLAPDAEEITDEAMKSLCWRSRSLYPEWTSKLEDLT
GLNTGYWPCGILAPVYEGQESKGVRIQENKGESPAYWLEKAVIHQYQPGLGEDVVGGWWYPEDAQVNNQALARVLWAAAE
SLGVELNDGITVEGLLQQQGQVVGVQTNTGIIQAEHYVLATGAWANELLPLPVTPRKGQMLRVRVPESVPELPLKRVLFG
ENIYIVPRRDRSIIIGATSEDVGFTPHNTPAGIQTLLQGAIRLYPQLQDYPIQEFWWGFRPATPDELPILGTSHCANLTL
ATGHYRNGILLAPITAALIADFIVEQKSDPLLSHFHYSRFQKQASTTPMFTHSANFSNGHAKNPPLPTLDSSLIIAGKSF
HSRLMTGTGKYRSIEEMQQSVVASGCEIVTVAVRRVQTKAPGHEGLAEALDWSRIWMLPNTAGCQTAEEAIRVARLGREM
AKLLGQEDNNFVKLEVIPDPKYLLPDPIGTLQAAEQLVKEGFAVLPYINADPMLAKRLEDVGCATVMPLASPIGSGQGLK
TTANIQIIIENAKIPVVVDAGIGAPSEASQAMELGADALLINSAIALAQNPAAMAQAMNLATVAGRLAYLAGRMPIKTYA
SASSPVTGTIS

Specific function: Catalyzes the rearrangement of 1-deoxy-D-xylulose 5- phosphate (DXP) to produce the thiazole phosphate moiety of thiamine. Sulfur is provided by the thiocarboxylate moiety of the carrier protein ThiS. In vitro, sulfur can be provided by H(2)S

COG id: COG0665

COG function: function code E; Glycine/D-amino acid oxidases (deaminating)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the thiG family

Homologues:

Organism=Escherichia coli, GI48994993, Length=256, Percent_Identity=53.515625, Blast_Score=253, Evalue=3e-68,
Organism=Escherichia coli, GI1787438, Length=250, Percent_Identity=28.8, Blast_Score=94, Evalue=3e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): THIOG_ANAVT (Q3M859)

Other databases:

- EMBL:   CP000117
- RefSeq:   YP_323722.1
- ProteinModelPortal:   Q3M859
- SMR:   Q3M859
- STRING:   Q3M859
- GeneID:   3680639
- GenomeReviews:   CP000117_GR
- KEGG:   ava:Ava_3219
- NMPDR:   fig|240292.3.peg.616
- eggNOG:   COG0665
- HOGENOM:   HBG438564
- OMA:   CQIVTVA
- PhylomeDB:   Q3M859
- ProtClustDB:   CLSK893498
- BioCyc:   AVAR240292:AVA_3219-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00443
- InterPro:   IPR013785
- InterPro:   IPR006076
- InterPro:   IPR012727
- InterPro:   IPR008867
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR02352

Pfam domain/function: PF01266 DAO; PF05690 ThiG; SSF110399 ThiG

EC number: NA

Molecular weight: Translated: 70274; Mature: 70142

Theoretical pI: Translated: 5.66; Mature: 5.66

Prosite motif: NA

Important sites: ACT_SITE 494-494 BINDING 52-52 BINDING 173-173 BINDING 301-301 BINDING 327-327 BINDING 555-555

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRDIVIIGGGVIGLAIAVELKLRGTKVTVLCRDFPAAAAHAAAGMLAPDAEEITDEAMK
CCCEEEEECCCCEEEEEEEEEEECCCEEEEEEECCCHHHHHHHHCCCCCCHHHHHHHHHH
SLCWRSRSLYPEWTSKLEDLTGLNTGYWPCGILAPVYEGQESKGVRIQENKGESPAYWLE
HHHHHCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCCCCEEEECCCCCCHHHHHH
KAVIHQYQPGLGEDVVGGWWYPEDAQVNNQALARVLWAAAESLGVELNDGITVEGLLQQQ
HHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCEECCCCCEEHHHHHCC
GQVVGVQTNTGIIQAEHYVLATGAWANELLPLPVTPRKGQMLRVRVPESVPELPLKRVLF
CCEEEEEECCCEEEECEEEEEECCCHHCCCCCCCCCCCCCEEEEECCCCCCCCCHHHHHC
GENIYIVPRRDRSIIIGATSEDVGFTPHNTPAGIQTLLQGAIRLYPQLQDYPIQEFWWGF
CCCEEEEECCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHCCC
RPATPDELPILGTSHCANLTLATGHYRNGILLAPITAALIADFIVEQKSDPLLSHFHYSR
CCCCCCCCCEEECCCCCCEEEEECCCCCCEEEHHHHHHHHHHHHHHCCCCHHHHHHHHHH
FQKQASTTPMFTHSANFSNGHAKNPPLPTLDSSLIIAGKSFHSRLMTGTGKYRSIEEMQQ
HHHHCCCCCEEEECCCCCCCCCCCCCCCCCCCCEEEECCHHHHHHHCCCCCCCCHHHHHH
SVVASGCEIVTVAVRRVQTKAPGHEGLAEALDWSRIWMLPNTAGCQTAEEAIRVARLGRE
HHHHCCCHHHHHHHHHHHHCCCCHHHHHHHCCCCEEEECCCCCCCHHHHHHHHHHHHHHH
MAKLLGQEDNNFVKLEVIPDPKYLLPDPIGTLQAAEQLVKEGFAVLPYINADPMLAKRLE
HHHHHCCCCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHCCCEEEEECCCCHHHHHHHH
DVGCATVMPLASPIGSGQGLKTTANIQIIIENAKIPVVVDAGIGAPSEASQAMELGADAL
HCCCHHHHHHHCCCCCCCCCEEEEEEEEEEECCCCCEEEECCCCCCHHHHHHHHHCCCHH
LINSAIALAQNPAAMAQAMNLATVAGRLAYLAGRMPIKTYASASSPVTGTIS
HHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCCCEECCC
>Mature Secondary Structure 
TRDIVIIGGGVIGLAIAVELKLRGTKVTVLCRDFPAAAAHAAAGMLAPDAEEITDEAMK
CCEEEEECCCCEEEEEEEEEEECCCEEEEEEECCCHHHHHHHHCCCCCCHHHHHHHHHH
SLCWRSRSLYPEWTSKLEDLTGLNTGYWPCGILAPVYEGQESKGVRIQENKGESPAYWLE
HHHHHCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCCCCEEEECCCCCCHHHHHH
KAVIHQYQPGLGEDVVGGWWYPEDAQVNNQALARVLWAAAESLGVELNDGITVEGLLQQQ
HHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCEECCCCCEEHHHHHCC
GQVVGVQTNTGIIQAEHYVLATGAWANELLPLPVTPRKGQMLRVRVPESVPELPLKRVLF
CCEEEEEECCCEEEECEEEEEECCCHHCCCCCCCCCCCCCEEEEECCCCCCCCCHHHHHC
GENIYIVPRRDRSIIIGATSEDVGFTPHNTPAGIQTLLQGAIRLYPQLQDYPIQEFWWGF
CCCEEEEECCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHCCC
RPATPDELPILGTSHCANLTLATGHYRNGILLAPITAALIADFIVEQKSDPLLSHFHYSR
CCCCCCCCCEEECCCCCCEEEEECCCCCCEEEHHHHHHHHHHHHHHCCCCHHHHHHHHHH
FQKQASTTPMFTHSANFSNGHAKNPPLPTLDSSLIIAGKSFHSRLMTGTGKYRSIEEMQQ
HHHHCCCCCEEEECCCCCCCCCCCCCCCCCCCCEEEECCHHHHHHHCCCCCCCCHHHHHH
SVVASGCEIVTVAVRRVQTKAPGHEGLAEALDWSRIWMLPNTAGCQTAEEAIRVARLGRE
HHHHCCCHHHHHHHHHHHHCCCCHHHHHHHCCCCEEEECCCCCCCHHHHHHHHHHHHHHH
MAKLLGQEDNNFVKLEVIPDPKYLLPDPIGTLQAAEQLVKEGFAVLPYINADPMLAKRLE
HHHHHCCCCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHCCCEEEEECCCCHHHHHHHH
DVGCATVMPLASPIGSGQGLKTTANIQIIIENAKIPVVVDAGIGAPSEASQAMELGADAL
HCCCHHHHHHHCCCCCCCCCEEEEEEEEEEECCCCCEEEECCCCCCHHHHHHHHHCCCHH
LINSAIALAQNPAAMAQAMNLATVAGRLAYLAGRMPIKTYASASSPVTGTIS
HHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCCCEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: deoxyxylulose-5-phosphate; ThiS-COSH; L-tyrosine [C]

Specific reaction: deoxyxylulose-5-phosphate + ThiS-COSH + L-tyrosine = 4-methyl-5-(beta-hydroxyethyl)thiazole phosphate + 4-hydroxy-benzyl-alcohol + C1 of tyrosine + ThiS protein [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA