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The map label for this gene is slt [H]

Identifier: 75909260

GI number: 75909260

Start: 3780878

End: 3783073

Strand: Direct

Name: slt [H]

Synonym: Ava_3051

Alternate gene names: 75909260

Gene position: 3780878-3783073 (Clockwise)

Preceding gene: 75909258

Following gene: 75909262

Centisome position: 59.39

GC content: 45.77

Gene sequence:

>2196_bases
ATGCTGAAAAAACTACAAAAAAAGCAAATTTCTATAATCGCTGGTGCGGCACTGTTTGCCTTTTCAGCTGGGGCGATGGT
GTCAGCACCGGAGATTGGTAAATCCTTGGGGCAATGGCTCAACGTGACTCAAGGTCAGCCCAAAGATTTATCCGAGGGAA
GTAGGGCGAAATCAGATGTATTTCCACTGATATCACAATCGCCGGTAGAACGAGCAGCCAAACTAGAAGAACTCTCGCAA
AACTCGCGATCGCCAGATCGAGAAAGAGCGCGTTATTTGTTGGCGAGTGATTATATAGACACAAAACAAGGCCAAAAAGC
CCTGGAACTACTCACGGGTTTAGAGAAAAGCTATCCTGTCCTCGCACCCTACATCTTACTGAAACAGGCACAAGCACAGG
ATTTATTAGGGGAAGATGGCAAAGCTTCTGACTTGAGGCAACAAGTATTAAAACAGTATCCCAAGGAAGCGGCGGTGGTG
AAAGCCATATATCTGATTGCTCAACCAAAGCTACAGGATACAGCGATCGCCCAATTTCCTTCCCATCCCCTCACCTGGGA
GATTATCAGGAAGCGCCTATCAGAAAATCCCAATCAGCCACAGTTGCAGCTCATCTTGGCTCAACACGCTTATACCCAGC
CAGGGATAGTAGGGGTGTTAGACGAGCTAGGAAAACAGACAAATCTCCAACCCCAAGACTGGGAAGTGATTGGTACAGCC
TACTGGGAGAATAATCAATTCCTCAAAGCGGCGAACGCTTACGCCAAAGCGCCAAAAACAGCCCGTAATCTCTACCGGAC
TGCGCGGGGATGGCAAGTAGGCGGGAAAAATCGAGAACAAGCAATCTCTACCTATAAGCAACTAGTACAGCAATTTCCTG
ATGCGCGAGAAACAGGACTAGGCTTGGTACGGTTGGCAGAAATGACTAAAACCAACAAAGATGCCTTACCCTATCTCAAC
CAAGTAATTGCTAAATTCCCTGAACAAGCAAGTCAGGCATTGGTAAAAAAAGCCGAAATCCTCACAGCCCTTAAGGATGA
AAAAGCAGCCCAACAAACTTGGCAACAACTTATTACCAAATATGCCAAATCCGATGAAGCCGCCGAATATCGGTGGAAAA
ATGCTTTAGAGAAAGCCAAAGCCAGAGATTACACCAGCGCTTGGAAGTGGGCGCAACCAATTGTGATCAATAACCCTAAC
AGTATTTTGGCTCCCAGGGCGGGTTTTTGGCTAGGTAAATGGGCAGCAGCCGTAGGGAAACAACAGGAAGCGCAAACTGC
CTATGAGTATGTGATTAGCCAGTTTCCTTACTCTTATTATGCCTGGCGATCGGCAAACCTCTTGGGGTTGAATGTCGGCA
ACTTTGATAACGTCCGTCAACTCACCCCAGAAGTAATTCCCTATCAACGCCCCATACCTCCTACAGGTTCCCCAGCCTTC
CAAGAATTGTATTTACTTGGTCAAGATCGTGACGCGTGGTTGCAATGGGAGACAGAATATCTCAATAAACAGGAACCCAC
AGTAGCAGAACAATTCACCGAAGGCTTGATGCGGTTAGCCAGAGGTGAATATCTGTCAGGAATTAACCTGATTTCTAAAC
TAGAAGATAGGGAAACACCAGAAGAACAAGCCGAATATCAGGCCTTAAGTAAACAAATTACCTACTGGCAAGCCCGTTAC
CCGTTCCCCTACTTGAAAGAAATTGAAAAATGGTCATCTGAGCGAGAACTTAATCCCTTGCTAGTTACCGCCTTAATGCG
TCAAGAATCCCGGTTTGAAGCAAAAATCAAATCCGTTGTCGGCGCGACTGGCTTAATGCAGGTAATGCCAGATACAGCTA
AGTGGATAGCATCAAAAATTCCCTTAGATATCAAGACCATTAATTTAGAGAATCCCAATGACAACGTGATGCTAGGGACA
TGGTATTTAGATCATACCCATGAGCAGTATGGTAATAATTCCATGTTAGCGATCGCTAGTTACAATGCTGGCCCAGGTAG
CGTCGCCCGATGGTTGAAAACTTTACCTAACCAAGACCCAGATGAGTTTGTTGAAGCCATCCCCTTTAATGAAACTAGGG
ATTATGTCCGCCAGGTATTTGGTAACTATTGGAATTATCTCCGACTATATAATCCTGAGATTGCCAGCATCGTAGCTAAA
TATTCCGCCGAACAACCGAAATTGCCCGGAAATTAG

Upstream 100 bases:

>100_bases
TTTTCCTAGAATAAGAAATTTCCAATGAAAAGGTGATTACACTCTCGGCAACTAATGCTAGAGTTTCAGGAAATTCATCG
GCACTGTTCAGCGAGTATCC

Downstream 100 bases:

>100_bases
GGAATAGGGGACGAGGAATTTTATGGTTGTTGATTGTTGACGGTTAACTGTCAACTGTCAACCGTCAACAAATTACACCA
ATTTGAAAAAAGAATGCGAC

Product: lytic transglycosylase catalytic subunit

Products: 1,6-Anhydrobond [C]

Alternate protein names: Exomuramidase; Peptidoglycan lytic exotransglycosylase; Slt70 [H]

Number of amino acids: Translated: 731; Mature: 731

Protein sequence:

>731_residues
MLKKLQKKQISIIAGAALFAFSAGAMVSAPEIGKSLGQWLNVTQGQPKDLSEGSRAKSDVFPLISQSPVERAAKLEELSQ
NSRSPDRERARYLLASDYIDTKQGQKALELLTGLEKSYPVLAPYILLKQAQAQDLLGEDGKASDLRQQVLKQYPKEAAVV
KAIYLIAQPKLQDTAIAQFPSHPLTWEIIRKRLSENPNQPQLQLILAQHAYTQPGIVGVLDELGKQTNLQPQDWEVIGTA
YWENNQFLKAANAYAKAPKTARNLYRTARGWQVGGKNREQAISTYKQLVQQFPDARETGLGLVRLAEMTKTNKDALPYLN
QVIAKFPEQASQALVKKAEILTALKDEKAAQQTWQQLITKYAKSDEAAEYRWKNALEKAKARDYTSAWKWAQPIVINNPN
SILAPRAGFWLGKWAAAVGKQQEAQTAYEYVISQFPYSYYAWRSANLLGLNVGNFDNVRQLTPEVIPYQRPIPPTGSPAF
QELYLLGQDRDAWLQWETEYLNKQEPTVAEQFTEGLMRLARGEYLSGINLISKLEDRETPEEQAEYQALSKQITYWQARY
PFPYLKEIEKWSSERELNPLLVTALMRQESRFEAKIKSVVGATGLMQVMPDTAKWIASKIPLDIKTINLENPNDNVMLGT
WYLDHTHEQYGNNSMLAIASYNAGPGSVARWLKTLPNQDPDEFVEAIPFNETRDYVRQVFGNYWNYLRLYNPEIASIVAK
YSAEQPKLPGN

Sequences:

>Translated_731_residues
MLKKLQKKQISIIAGAALFAFSAGAMVSAPEIGKSLGQWLNVTQGQPKDLSEGSRAKSDVFPLISQSPVERAAKLEELSQ
NSRSPDRERARYLLASDYIDTKQGQKALELLTGLEKSYPVLAPYILLKQAQAQDLLGEDGKASDLRQQVLKQYPKEAAVV
KAIYLIAQPKLQDTAIAQFPSHPLTWEIIRKRLSENPNQPQLQLILAQHAYTQPGIVGVLDELGKQTNLQPQDWEVIGTA
YWENNQFLKAANAYAKAPKTARNLYRTARGWQVGGKNREQAISTYKQLVQQFPDARETGLGLVRLAEMTKTNKDALPYLN
QVIAKFPEQASQALVKKAEILTALKDEKAAQQTWQQLITKYAKSDEAAEYRWKNALEKAKARDYTSAWKWAQPIVINNPN
SILAPRAGFWLGKWAAAVGKQQEAQTAYEYVISQFPYSYYAWRSANLLGLNVGNFDNVRQLTPEVIPYQRPIPPTGSPAF
QELYLLGQDRDAWLQWETEYLNKQEPTVAEQFTEGLMRLARGEYLSGINLISKLEDRETPEEQAEYQALSKQITYWQARY
PFPYLKEIEKWSSERELNPLLVTALMRQESRFEAKIKSVVGATGLMQVMPDTAKWIASKIPLDIKTINLENPNDNVMLGT
WYLDHTHEQYGNNSMLAIASYNAGPGSVARWLKTLPNQDPDEFVEAIPFNETRDYVRQVFGNYWNYLRLYNPEIASIVAK
YSAEQPKLPGN
>Mature_731_residues
MLKKLQKKQISIIAGAALFAFSAGAMVSAPEIGKSLGQWLNVTQGQPKDLSEGSRAKSDVFPLISQSPVERAAKLEELSQ
NSRSPDRERARYLLASDYIDTKQGQKALELLTGLEKSYPVLAPYILLKQAQAQDLLGEDGKASDLRQQVLKQYPKEAAVV
KAIYLIAQPKLQDTAIAQFPSHPLTWEIIRKRLSENPNQPQLQLILAQHAYTQPGIVGVLDELGKQTNLQPQDWEVIGTA
YWENNQFLKAANAYAKAPKTARNLYRTARGWQVGGKNREQAISTYKQLVQQFPDARETGLGLVRLAEMTKTNKDALPYLN
QVIAKFPEQASQALVKKAEILTALKDEKAAQQTWQQLITKYAKSDEAAEYRWKNALEKAKARDYTSAWKWAQPIVINNPN
SILAPRAGFWLGKWAAAVGKQQEAQTAYEYVISQFPYSYYAWRSANLLGLNVGNFDNVRQLTPEVIPYQRPIPPTGSPAF
QELYLLGQDRDAWLQWETEYLNKQEPTVAEQFTEGLMRLARGEYLSGINLISKLEDRETPEEQAEYQALSKQITYWQARY
PFPYLKEIEKWSSERELNPLLVTALMRQESRFEAKIKSVVGATGLMQVMPDTAKWIASKIPLDIKTINLENPNDNVMLGT
WYLDHTHEQYGNNSMLAIASYNAGPGSVARWLKTLPNQDPDEFVEAIPFNETRDYVRQVFGNYWNYLRLYNPEIASIVAK
YSAEQPKLPGN

Specific function: Murein-degrading enzyme. Catalyzes the cleavage of the glycosidic bonds between N-acetylmuramic acid and N- acetylglucosamine residues in peptidoglycan. May play a role in recycling of muropeptides during cell elongation and/or cell division [H]

COG id: COG0741

COG function: function code M; Soluble lytic murein transglycosylase and related regulatory proteins (some contain LysM/invasin domains)

Gene ontology:

Cell location: Periplasm. Note=Tightly associated with the murein sacculus (By similarity) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transglycosylase slt family [H]

Homologues:

Organism=Escherichia coli, GI87082441, Length=163, Percent_Identity=39.2638036809816, Blast_Score=113, Evalue=3e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016026
- InterPro:   IPR008258
- InterPro:   IPR012289
- InterPro:   IPR008939
- InterPro:   IPR000189 [H]

Pfam domain/function: PF01464 SLT [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 82786; Mature: 82786

Theoretical pI: Translated: 9.19; Mature: 9.19

Prosite motif: PS00922 TRANSGLYCOSYLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKKLQKKQISIIAGAALFAFSAGAMVSAPEIGKSLGQWLNVTQGQPKDLSEGSRAKSDV
CCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHH
FPLISQSPVERAAKLEELSQNSRSPDRERARYLLASDYIDTKQGQKALELLTGLEKSYPV
HHHHCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCH
LAPYILLKQAQAQDLLGEDGKASDLRQQVLKQYPKEAAVVKAIYLIAQPKLQDTAIAQFP
HHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCHHHHHHCC
SHPLTWEIIRKRLSENPNQPQLQLILAQHAYTQPGIVGVLDELGKQTNLQPQDWEVIGTA
CCCCHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCHHHHHHHHCHHCCCCCCCCEEEEEE
YWENNQFLKAANAYAKAPKTARNLYRTARGWQVGGKNREQAISTYKQLVQQFPDARETGL
EECCCCHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHCC
GLVRLAEMTKTNKDALPYLNQVIAKFPEQASQALVKKAEILTALKDEKAAQQTWQQLITK
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YAKSDEAAEYRWKNALEKAKARDYTSAWKWAQPIVINNPNSILAPRAGFWLGKWAAAVGK
HHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCHHHHHHHHHHCC
QQEAQTAYEYVISQFPYSYYAWRSANLLGLNVGNFDNVRQLTPEVIPYQRPIPPTGSPAF
HHHHHHHHHHHHHHCCCHHEEECCCCEEEEECCCCCHHHHHCHHHCCCCCCCCCCCCHHH
QELYLLGQDRDAWLQWETEYLNKQEPTVAEQFTEGLMRLARGEYLSGINLISKLEDRETP
HHHHHHCCCCCHHEEEEHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
EEQAEYQALSKQITYWQARYPFPYLKEIEKWSSERELNPLLVTALMRQESRFEAKIKSVV
HHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
GATGLMQVMPDTAKWIASKIPLDIKTINLENPNDNVMLGTWYLDHTHEQYGNNSMLAIAS
HHHHHHHHCCHHHHHHHHHCCCEEEEEEECCCCCCEEEEEEEECCCHHHHCCCCEEEEEE
YNAGPGSVARWLKTLPNQDPDEFVEAIPFNETRDYVRQVFGNYWNYLRLYNPEIASIVAK
CCCCCHHHHHHHHHCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHEECCHHHHHHHHH
YSAEQPKLPGN
HCCCCCCCCCC
>Mature Secondary Structure
MLKKLQKKQISIIAGAALFAFSAGAMVSAPEIGKSLGQWLNVTQGQPKDLSEGSRAKSDV
CCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHH
FPLISQSPVERAAKLEELSQNSRSPDRERARYLLASDYIDTKQGQKALELLTGLEKSYPV
HHHHCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCH
LAPYILLKQAQAQDLLGEDGKASDLRQQVLKQYPKEAAVVKAIYLIAQPKLQDTAIAQFP
HHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCHHHHHHCC
SHPLTWEIIRKRLSENPNQPQLQLILAQHAYTQPGIVGVLDELGKQTNLQPQDWEVIGTA
CCCCHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCHHHHHHHHCHHCCCCCCCCEEEEEE
YWENNQFLKAANAYAKAPKTARNLYRTARGWQVGGKNREQAISTYKQLVQQFPDARETGL
EECCCCHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHCC
GLVRLAEMTKTNKDALPYLNQVIAKFPEQASQALVKKAEILTALKDEKAAQQTWQQLITK
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YAKSDEAAEYRWKNALEKAKARDYTSAWKWAQPIVINNPNSILAPRAGFWLGKWAAAVGK
HHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCHHHHHHHHHHCC
QQEAQTAYEYVISQFPYSYYAWRSANLLGLNVGNFDNVRQLTPEVIPYQRPIPPTGSPAF
HHHHHHHHHHHHHHCCCHHEEECCCCEEEEECCCCCHHHHHCHHHCCCCCCCCCCCCHHH
QELYLLGQDRDAWLQWETEYLNKQEPTVAEQFTEGLMRLARGEYLSGINLISKLEDRETP
HHHHHHCCCCCHHEEEEHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
EEQAEYQALSKQITYWQARYPFPYLKEIEKWSSERELNPLLVTALMRQESRFEAKIKSVV
HHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
GATGLMQVMPDTAKWIASKIPLDIKTINLENPNDNVMLGTWYLDHTHEQYGNNSMLAIAS
HHHHHHHHCCHHHHHHHHHCCCEEEEEEECCCCCCEEEEEEEECCCHHHHCCCCEEEEEE
YNAGPGSVARWLKTLPNQDPDEFVEAIPFNETRDYVRQVFGNYWNYLRLYNPEIASIVAK
CCCCCHHHHHHHHHCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHEECCHHHHHHHHH
YSAEQPKLPGN
HCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Muramic Acid Residue (N-Acetylmuramic Acid And N-Acetylglucosamine Residues) [C]

Specific reaction: Cleavage Of The Beta-1,4-Glycosidic Bond Between N-Acetylmuramic Acid And N-Acetylglucosamine Residues, Thereby Conserving The Energy In A Newly Synthesized 1,6-Anhydrobond In The Muramic Acid Residue. [C]

General reaction: Cleavage Of The Beta-1,4-Glycosidic Bond [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]