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The map label for this gene is gacS [H]

Identifier: 75909242

GI number: 75909242

Start: 3755798

End: 3757555

Strand: Direct

Name: gacS [H]

Synonym: Ava_3033

Alternate gene names: 75909242

Gene position: 3755798-3757555 (Clockwise)

Preceding gene: 75909241

Following gene: 75909243

Centisome position: 59.0

GC content: 42.55

Gene sequence:

>1758_bases
ATGACTATTACTGCCAATTCCCAATTACCAAATAGATTTCCCCAAAATCTAGAATCGATGAACCACAAGGCTGATGGATT
ATTAGCAACCCTCGGCGCTGAGTTGGTATATGTGCAGGATGGGGTGGGGCGTTACCTGTCTTTTTATTGGCAGAAAAGTG
AACTTTTGGGTTTCAACCAAGAGCAAATAGTATGCGATGGTAATGGACAGGAAATTTTTGTCCCACTGGACAAAGTTTCT
TACATGAAGAGACTGCACTATGTCATGGCTAACTTAGCTCCAGAAAAGTTCTCTTGCTGGTTTAGCTGTCATCAAAATCT
ATTTGAGTTGGAACTGGCTATCACGCCGATTATTCCACCTTTGGGAACTGCTGCCACGACGGTCTTGGTAATGGGAAGGT
TATTACAAGTTAAAGTAAATAACCAAGAGGTCAATGTAGCAATTACCACCCCTACACAAACAGACCTAGCTTCGCGATCG
CAGCAACACCAAAGATTGGTAAATAAAATTACCAGAAATATTCGCCGGACACTAGATTTAGACGTTATTTGGCAACAAAC
AGTTGACAGTCTAGGTAAAGCACTCAGGCTAGAGCGCTGTATTATTTGTCCTTACCAGCCCTCTAGCTCTAAAGTGCGAG
TAATTGCCGAATATCATCAACCAGATCGTCCTTCCATGCTGGGTTTAGAAATAGATGTGACTTCTGAACCAGCTTTTGCT
CAGGCGTTGACAACCTTGGAACCAATCATTATGGAAGTTCCTGGTTATAAACTTTGCCCACAACAGAAAATTTTAGTAGT
AGCCACTTGTTACCAAGACCAAGCTAATGGCTTAATAGCGATCAATTTACGGCATGAATGTTATCCCTTAACCAATGCCG
AATTAGAACTAGCTAAAGAAGTAGCAGATCAGTTGGGAACAGCGATCGCTCATGCTACCTTATATAAAGAATTAGAAGCA
GCACGACAAAAAGCTGAAGAAGCTTCCCGCCTCAAGAGTGAGTTTCTCGCTAATGTCTCCCATGAAATCCGCACCCCCTT
GAATGGGATGATTGGTTTCCTCAAGCTGATTTTAGAAGGTATGGCAGACGATGTCGAAGAAAAAAACCAGTTTTTATCGG
AAGCTCATCAATTATCTTTACATCTACTCAACATTATTAACGACATCTTAGATATAGCTAAAATCGAAGCTGGCAAAATG
GAGTTGGTGTGCGCTCCCATCAAGCTGGAAGAGCTATTCTCTGATGTAGATAACTTTATGCGTCCACAAGCAGAAGTCAA
AAACCTCAGTTTCCGGATGCAATTGCCACCAACCTCCGATGAAATCGTTGTGCAAGGCAATTACCAAAGGCTTTTACAAG
TCATGTTAAATATAGTCGGGAATGCGATTAAATTTACCCACGAAGGTGGTATTACCGTTTCTGCCGACGTTGTCCTGAAA
AAAGGGAAACTCCAAGAACAGCACTTTCCTGGTATGGTGAGAGTCAGAATAGCCGATACAGGCATTGGTGTTTCCTTAGA
CCAGCAAGACAAATTATTTCAATTATTCTCTCAAGTAGATGGTTCCCGTACTCGTCAGTATGGTGGTACAGGTTTGGGAC
TGGCAATATCCCAAAAACTTGTAGAGACGATGGGTGGCGAAGTGCATTTTTACAGTCTTGGTGAGGGACTTGGTTCGACA
GTCACATTTACCGTACCTCTATACCAACAGCCAGTCATGCTTTCCTCTAACGACGCTGATGCTGAGTGCATCGGTTAA

Upstream 100 bases:

>100_bases
ACTCAGTATGTAAAATTGGTCAAATTTTACGCCAAGCTTTGCCCAGCCTTAGTAAGACTGTTTTGAGTTGCCATTAGTCC
ATCCAACTCGATTCAACGTA

Downstream 100 bases:

>100_bases
GTCTAGGACTTATGTACTCAGGGATTAGGTGTAAGGGTATAAGGGTGTGAGGGTGTAAGGGTGTGAGGGTGTAAGGGTAT
GAGGGTTTTAAACATTTACA

Product: GAF sensor signal transduction histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 585; Mature: 584

Protein sequence:

>585_residues
MTITANSQLPNRFPQNLESMNHKADGLLATLGAELVYVQDGVGRYLSFYWQKSELLGFNQEQIVCDGNGQEIFVPLDKVS
YMKRLHYVMANLAPEKFSCWFSCHQNLFELELAITPIIPPLGTAATTVLVMGRLLQVKVNNQEVNVAITTPTQTDLASRS
QQHQRLVNKITRNIRRTLDLDVIWQQTVDSLGKALRLERCIICPYQPSSSKVRVIAEYHQPDRPSMLGLEIDVTSEPAFA
QALTTLEPIIMEVPGYKLCPQQKILVVATCYQDQANGLIAINLRHECYPLTNAELELAKEVADQLGTAIAHATLYKELEA
ARQKAEEASRLKSEFLANVSHEIRTPLNGMIGFLKLILEGMADDVEEKNQFLSEAHQLSLHLLNIINDILDIAKIEAGKM
ELVCAPIKLEELFSDVDNFMRPQAEVKNLSFRMQLPPTSDEIVVQGNYQRLLQVMLNIVGNAIKFTHEGGITVSADVVLK
KGKLQEQHFPGMVRVRIADTGIGVSLDQQDKLFQLFSQVDGSRTRQYGGTGLGLAISQKLVETMGGEVHFYSLGEGLGST
VTFTVPLYQQPVMLSSNDADAECIG

Sequences:

>Translated_585_residues
MTITANSQLPNRFPQNLESMNHKADGLLATLGAELVYVQDGVGRYLSFYWQKSELLGFNQEQIVCDGNGQEIFVPLDKVS
YMKRLHYVMANLAPEKFSCWFSCHQNLFELELAITPIIPPLGTAATTVLVMGRLLQVKVNNQEVNVAITTPTQTDLASRS
QQHQRLVNKITRNIRRTLDLDVIWQQTVDSLGKALRLERCIICPYQPSSSKVRVIAEYHQPDRPSMLGLEIDVTSEPAFA
QALTTLEPIIMEVPGYKLCPQQKILVVATCYQDQANGLIAINLRHECYPLTNAELELAKEVADQLGTAIAHATLYKELEA
ARQKAEEASRLKSEFLANVSHEIRTPLNGMIGFLKLILEGMADDVEEKNQFLSEAHQLSLHLLNIINDILDIAKIEAGKM
ELVCAPIKLEELFSDVDNFMRPQAEVKNLSFRMQLPPTSDEIVVQGNYQRLLQVMLNIVGNAIKFTHEGGITVSADVVLK
KGKLQEQHFPGMVRVRIADTGIGVSLDQQDKLFQLFSQVDGSRTRQYGGTGLGLAISQKLVETMGGEVHFYSLGEGLGST
VTFTVPLYQQPVMLSSNDADAECIG
>Mature_584_residues
TITANSQLPNRFPQNLESMNHKADGLLATLGAELVYVQDGVGRYLSFYWQKSELLGFNQEQIVCDGNGQEIFVPLDKVSY
MKRLHYVMANLAPEKFSCWFSCHQNLFELELAITPIIPPLGTAATTVLVMGRLLQVKVNNQEVNVAITTPTQTDLASRSQ
QHQRLVNKITRNIRRTLDLDVIWQQTVDSLGKALRLERCIICPYQPSSSKVRVIAEYHQPDRPSMLGLEIDVTSEPAFAQ
ALTTLEPIIMEVPGYKLCPQQKILVVATCYQDQANGLIAINLRHECYPLTNAELELAKEVADQLGTAIAHATLYKELEAA
RQKAEEASRLKSEFLANVSHEIRTPLNGMIGFLKLILEGMADDVEEKNQFLSEAHQLSLHLLNIINDILDIAKIEAGKME
LVCAPIKLEELFSDVDNFMRPQAEVKNLSFRMQLPPTSDEIVVQGNYQRLLQVMLNIVGNAIKFTHEGGITVSADVVLKK
GKLQEQHFPGMVRVRIADTGIGVSLDQQDKLFQLFSQVDGSRTRQYGGTGLGLAISQKLVETMGGEVHFYSLGEGLGSTV
TFTVPLYQQPVMLSSNDADAECIG

Specific function: Forms part of a two-component regulatory system gacA/gacS(lemA). May be involved in lesion formation, swarming and in the production of extracellular protease, syringomycin and N- acyl-L-homoserine lactone (acyl-HSL). Required for pathogenicity on bean [H

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1789149, Length=255, Percent_Identity=41.5686274509804, Blast_Score=181, Evalue=1e-46,
Organism=Escherichia coli, GI48994928, Length=242, Percent_Identity=35.1239669421488, Blast_Score=148, Evalue=9e-37,
Organism=Escherichia coli, GI1788713, Length=256, Percent_Identity=33.984375, Blast_Score=131, Evalue=1e-31,
Organism=Escherichia coli, GI145693157, Length=262, Percent_Identity=34.3511450381679, Blast_Score=125, Evalue=8e-30,
Organism=Escherichia coli, GI87081816, Length=257, Percent_Identity=35.408560311284, Blast_Score=119, Evalue=4e-28,
Organism=Escherichia coli, GI1788393, Length=244, Percent_Identity=31.1475409836066, Blast_Score=102, Evalue=5e-23,
Organism=Escherichia coli, GI1790346, Length=242, Percent_Identity=28.5123966942149, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1786600, Length=275, Percent_Identity=27.2727272727273, Blast_Score=84, Evalue=4e-17,
Organism=Escherichia coli, GI1786912, Length=269, Percent_Identity=27.1375464684015, Blast_Score=81, Evalue=2e-16,
Organism=Escherichia coli, GI1790436, Length=267, Percent_Identity=27.7153558052434, Blast_Score=76, Evalue=5e-15,
Organism=Escherichia coli, GI1788549, Length=296, Percent_Identity=25.3378378378378, Blast_Score=76, Evalue=7e-15,
Organism=Escherichia coli, GI1786783, Length=244, Percent_Identity=24.1803278688525, Blast_Score=72, Evalue=1e-13,
Organism=Escherichia coli, GI1787894, Length=247, Percent_Identity=25.5060728744939, Blast_Score=69, Evalue=6e-13,
Organism=Escherichia coli, GI1790861, Length=244, Percent_Identity=25.4098360655738, Blast_Score=64, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR011006
- InterPro:   IPR003660
- InterPro:   IPR004358
- InterPro:   IPR008207
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF00512 HisKA; PF01627 Hpt; PF00072 Response_reg [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 65082; Mature: 64951

Theoretical pI: Translated: 5.26; Mature: 5.26

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTITANSQLPNRFPQNLESMNHKADGLLATLGAELVYVQDGVGRYLSFYWQKSELLGFNQ
CEEECCCCCCCCCHHHHHHCCCCCCCHHHHHCCEEEEEECCHHHHHHHHHHHHHHCCCCC
EQIVCDGNGQEIFVPLDKVSYMKRLHYVMANLAPEKFSCWFSCHQNLFELELAITPIIPP
CEEEECCCCCEEEEEHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCEEEEEEEECCCCC
LGTAATTVLVMGRLLQVKVNNQEVNVAITTPTQTDLASRSQQHQRLVNKITRNIRRTLDL
CCHHHHHHHHHHHHEEEEECCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
DVIWQQTVDSLGKALRLERCIICPYQPSSSKVRVIAEYHQPDRPSMLGLEIDVTSEPAFA
HHHHHHHHHHHHHHHHHCCEEECCCCCCCCCEEEEEECCCCCCCCEEEEEEEECCCCHHH
QALTTLEPIIMEVPGYKLCPQQKILVVATCYQDQANGLIAINLRHECYPLTNAELELAKE
HHHHHHHHHHHCCCCCCCCCCCCEEEEEEECCCCCCCEEEEEECCCEECCCCCHHHHHHH
VADQLGTAIAHATLYKELEAARQKAEEASRLKSEFLANVSHEIRTPLNGMIGFLKLILEG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
MADDVEEKNQFLSEAHQLSLHLLNIINDILDIAKIEAGKMELVCAPIKLEELFSDVDNFM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHH
RPQAEVKNLSFRMQLPPTSDEIVVQGNYQRLLQVMLNIVGNAIKFTHEGGITVSADVVLK
CCHHHHCCCEEEEECCCCCCCEEEECCHHHHHHHHHHHHCCCEEEEECCCEEEEEHEEEE
KGKLQEQHFPGMVRVRIADTGIGVSLDQQDKLFQLFSQVDGSRTRQYGGTGLGLAISQKL
CCCCCHHCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHCCCCHHHHCCCCCCHHHHHHHH
VETMGGEVHFYSLGEGLGSTVTFTVPLYQQPVMLSSNDADAECIG
HHHHCCCEEEEECCCCCCCEEEEEEECCCCCEEEECCCCCCCCCC
>Mature Secondary Structure 
TITANSQLPNRFPQNLESMNHKADGLLATLGAELVYVQDGVGRYLSFYWQKSELLGFNQ
EEECCCCCCCCCHHHHHHCCCCCCCHHHHHCCEEEEEECCHHHHHHHHHHHHHHCCCCC
EQIVCDGNGQEIFVPLDKVSYMKRLHYVMANLAPEKFSCWFSCHQNLFELELAITPIIPP
CEEEECCCCCEEEEEHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCEEEEEEEECCCCC
LGTAATTVLVMGRLLQVKVNNQEVNVAITTPTQTDLASRSQQHQRLVNKITRNIRRTLDL
CCHHHHHHHHHHHHEEEEECCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
DVIWQQTVDSLGKALRLERCIICPYQPSSSKVRVIAEYHQPDRPSMLGLEIDVTSEPAFA
HHHHHHHHHHHHHHHHHCCEEECCCCCCCCCEEEEEECCCCCCCCEEEEEEEECCCCHHH
QALTTLEPIIMEVPGYKLCPQQKILVVATCYQDQANGLIAINLRHECYPLTNAELELAKE
HHHHHHHHHHHCCCCCCCCCCCCEEEEEEECCCCCCCEEEEEECCCEECCCCCHHHHHHH
VADQLGTAIAHATLYKELEAARQKAEEASRLKSEFLANVSHEIRTPLNGMIGFLKLILEG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
MADDVEEKNQFLSEAHQLSLHLLNIINDILDIAKIEAGKMELVCAPIKLEELFSDVDNFM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHH
RPQAEVKNLSFRMQLPPTSDEIVVQGNYQRLLQVMLNIVGNAIKFTHEGGITVSADVVLK
CCHHHHCCCEEEEECCCCCCCEEEECCHHHHHHHHHHHHCCCEEEEECCCEEEEEHEEEE
KGKLQEQHFPGMVRVRIADTGIGVSLDQQDKLFQLFSQVDGSRTRQYGGTGLGLAISQKL
CCCCCHHCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHCCCCHHHHCCCCCCHHHHHHHH
VETMGGEVHFYSLGEGLGSTVTFTVPLYQQPVMLSSNDADAECIG
HHHHCCCEEEEECCCCCCCEEEEEEECCCCCEEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1314807 [H]