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The map label for this gene is purT

Identifier: 75909231

GI number: 75909231

Start: 3745640

End: 3746815

Strand: Direct

Name: purT

Synonym: Ava_3022

Alternate gene names: 75909231

Gene position: 3745640-3746815 (Clockwise)

Preceding gene: 75909230

Following gene: 75909232

Centisome position: 58.84

GC content: 42.43

Gene sequence:

>1176_bases
ATGAGTAAGGCAATTAAGCTACCCCAAAAACTAATGTTATTGGGGTCAGGTGAGTTGGGCAAAGAATTTGTCATTGCGGC
TCAACGTCTTGGTAATTATGTGATAGCCGTTGACCGCTACGCCAACGCACCCGCGATGCAGGTTGCTGATTTTTGTGAAG
TAATATCAATGCTTAGTGCTGATGATTTAGAAGCTGTAGTCACTAAGTATCAACCTGATTTTATCATACCAGAGATTGAA
GCAATTAGAACCGAGAAACTACAGGAATTTGAAGACAGAGGTATCACTGTCATCCCCACTGCGGCAGCTACGAATTATAC
AATGAACCGCGATCGCATTCGGGAATTAGCCCATGAAGAATTAGGTATTAGAACCGCGAAATATGGTTATGCTATTACTT
TAGAAGAATTGATTGCTGTTTCTGATGAGATAGGTTTTCCCAATGTAGTCAAACCTGTGATGTCGTCCTCAGGTAAGGGA
CAATCGGTAGTAGCTACAAAAGAGGAAGTAGAAAAAGCTTGGAATTATGCGATCGCTAATTCTCGTGGTGATAGTCAAAA
AGTCATCGTCGAAGAATTTATCAACTTTGAAATTGAAATAACTCTCCTCACCATTAAACAGTGGGATAGTCCGACAATTT
TCTGTTCACCCATCGGACACCGTCAAGAACGAGGCGATTATCAAGAATCTTGGCAACCGGCCGGCATTAGTGAAGATAAG
ATATTAGAAGCGCAAGCCATAGCCAAAAAAGTCACAGATGCTTTAGGCGGTGCTGGCATATTTGGGGTAGAATTTTTCAT
TACCAAAGACGAAGTAATCTTTTCTGAACTATCACCTCGTCCCCACGATACGGGAATGGTGACATTAATTTCACAAAATT
TGAACGAATTTGAGTTACATTTACGAGCAATTTTAGGCTTACCCATTCCCAAAATAGAACAGTTGGGGCCTTCAGCCAGT
GCCGTAATTTTAGCATCAGAAAAATTAGATGCACCCGTGTTTACGGGTATCGCCGAAGCATTAGCCGCACCAGACGTAGA
TATTAGACTATTTGGTAAACCCACAGCACATCCTTACCGCCGTATGGGTGTCGCCTTAGCCAAAGCCGAGAATGTGCAAG
CCGCTAGGGAGAAAGCGACAGACGCAGCAAGTAAAATCCAAATCAGTAGTCAATAA

Upstream 100 bases:

>100_bases
TTACAACTGCGGGGAGCGAAGTTCAACTATTTGGCAGATGATAATTAAATTGCAGTATTAACTATTAGGCTGCTGGATTT
TATTTATCTGGGAAAGGTGC

Downstream 100 bases:

>100_bases
TTGCTGACTACTGCAAAAAAATTTTCAAAGTTCCTAAAAATGTCCATATCTCAAAGGAAAAATTTAGTTATGCAGGCACT
ATCCGATGAGATTATAATTA

Product: phosphoribosylglycinamide formyltransferase 2

Products: 5'-Phosphoribosyl-N-Formylglycinamide; Pyrophosphate; acetylphosphate; ADP [C]

Alternate protein names: GART 2; 5'-phosphoribosylglycinamide transformylase 2; Formate-dependent GAR transformylase; GAR transformylase 2

Number of amino acids: Translated: 391; Mature: 390

Protein sequence:

>391_residues
MSKAIKLPQKLMLLGSGELGKEFVIAAQRLGNYVIAVDRYANAPAMQVADFCEVISMLSADDLEAVVTKYQPDFIIPEIE
AIRTEKLQEFEDRGITVIPTAAATNYTMNRDRIRELAHEELGIRTAKYGYAITLEELIAVSDEIGFPNVVKPVMSSSGKG
QSVVATKEEVEKAWNYAIANSRGDSQKVIVEEFINFEIEITLLTIKQWDSPTIFCSPIGHRQERGDYQESWQPAGISEDK
ILEAQAIAKKVTDALGGAGIFGVEFFITKDEVIFSELSPRPHDTGMVTLISQNLNEFELHLRAILGLPIPKIEQLGPSAS
AVILASEKLDAPVFTGIAEALAAPDVDIRLFGKPTAHPYRRMGVALAKAENVQAAREKATDAASKIQISSQ

Sequences:

>Translated_391_residues
MSKAIKLPQKLMLLGSGELGKEFVIAAQRLGNYVIAVDRYANAPAMQVADFCEVISMLSADDLEAVVTKYQPDFIIPEIE
AIRTEKLQEFEDRGITVIPTAAATNYTMNRDRIRELAHEELGIRTAKYGYAITLEELIAVSDEIGFPNVVKPVMSSSGKG
QSVVATKEEVEKAWNYAIANSRGDSQKVIVEEFINFEIEITLLTIKQWDSPTIFCSPIGHRQERGDYQESWQPAGISEDK
ILEAQAIAKKVTDALGGAGIFGVEFFITKDEVIFSELSPRPHDTGMVTLISQNLNEFELHLRAILGLPIPKIEQLGPSAS
AVILASEKLDAPVFTGIAEALAAPDVDIRLFGKPTAHPYRRMGVALAKAENVQAAREKATDAASKIQISSQ
>Mature_390_residues
SKAIKLPQKLMLLGSGELGKEFVIAAQRLGNYVIAVDRYANAPAMQVADFCEVISMLSADDLEAVVTKYQPDFIIPEIEA
IRTEKLQEFEDRGITVIPTAAATNYTMNRDRIRELAHEELGIRTAKYGYAITLEELIAVSDEIGFPNVVKPVMSSSGKGQ
SVVATKEEVEKAWNYAIANSRGDSQKVIVEEFINFEIEITLLTIKQWDSPTIFCSPIGHRQERGDYQESWQPAGISEDKI
LEAQAIAKKVTDALGGAGIFGVEFFITKDEVIFSELSPRPHDTGMVTLISQNLNEFELHLRAILGLPIPKIEQLGPSASA
VILASEKLDAPVFTGIAEALAAPDVDIRLFGKPTAHPYRRMGVALAKAENVQAAREKATDAASKIQISSQ

Specific function: Catalyzes two reactions:the first one is the production of beta-formyl glycinamide ribonucleotide (GAR) from formate, ATP and beta GAR; the second, a side reaction, is the production of acetyl phosphate and ADP from acetate and ATP

COG id: COG0027

COG function: function code F; Formate-dependent phosphoribosylglycinamide formyltransferase (GAR transformylase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain

Homologues:

Organism=Escherichia coli, GI1788155, Length=381, Percent_Identity=54.8556430446194, Blast_Score=392, Evalue=1e-110,
Organism=Escherichia coli, GI1786733, Length=329, Percent_Identity=25.531914893617, Blast_Score=72, Evalue=5e-14,
Organism=Escherichia coli, GI1786216, Length=163, Percent_Identity=26.3803680981595, Blast_Score=62, Evalue=8e-11,
Organism=Saccharomyces cerevisiae, GI6324702, Length=370, Percent_Identity=25.1351351351351, Blast_Score=105, Evalue=1e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PURT_ANAVT (Q3M8Q4)

Other databases:

- EMBL:   CP000117
- RefSeq:   YP_323527.1
- ProteinModelPortal:   Q3M8Q4
- SMR:   Q3M8Q4
- STRING:   Q3M8Q4
- GeneID:   3681199
- GenomeReviews:   CP000117_GR
- KEGG:   ava:Ava_3022
- NMPDR:   fig|240292.3.peg.2246
- eggNOG:   COG0027
- HOGENOM:   HBG516369
- OMA:   TMNRKAI
- PhylomeDB:   Q3M8Q4
- ProtClustDB:   PRK09288
- BioCyc:   AVAR240292:AVA_3022-MONOMER
- HAMAP:   MF_01643
- InterPro:   IPR011761
- InterPro:   IPR003135
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR005862
- InterPro:   IPR011054
- Gene3D:   G3DSA:3.30.1490.20
- Gene3D:   G3DSA:3.30.470.20
- Gene3D:   G3DSA:3.40.50.20
- PANTHER:   PTHR23047:SF2
- TIGRFAMs:   TIGR01142

Pfam domain/function: PF02222 ATP-grasp; SSF52440 PreATP-grasp-like; SSF51246 Rudmnt_hyb_motif

EC number: 2.1.2.- [C]

Molecular weight: Translated: 42833; Mature: 42702

Theoretical pI: Translated: 4.69; Mature: 4.69

Prosite motif: PS50975 ATP_GRASP

Important sites: BINDING 78-78 BINDING 110-110 BINDING 151-151 BINDING 199-199 BINDING 264-264 BINDING 276-276 BINDING 283-283 BINDING 353-353

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKAIKLPQKLMLLGSGELGKEFVIAAQRLGNYVIAVDRYANAPAMQVADFCEVISMLSA
CCCCHHCCHHHEEECCCCCCHHHHHHHHHHCCEEEEEECCCCCCHHHHHHHHHHHHHHCC
DDLEAVVTKYQPDFIIPEIEAIRTEKLQEFEDRGITVIPTAAATNYTMNRDRIRELAHEE
CHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCHHHHHHHHHHH
LGIRTAKYGYAITLEELIAVSDEIGFPNVVKPVMSSSGKGQSVVATKEEVEKAWNYAIAN
HCCEEECCCEEEEHHHHHHHHCCCCCCHHHHHHHCCCCCCCEEEECHHHHHHHHCCCEEC
SRGDSQKVIVEEFINFEIEITLLTIKQWDSPTIFCSPIGHRQERGDYQESWQPAGISEDK
CCCCCHHHHHHHHCCEEEEEEEEEEEECCCCEEEECCCCCCHHCCCHHHCCCCCCCCHHH
ILEAQAIAKKVTDALGGAGIFGVEFFITKDEVIFSELSPRPHDTGMVTLISQNLNEFELH
HHHHHHHHHHHHHHHCCCCEEEEEEEEECCHHHHHHCCCCCCCCCEEEEECCCCCHHHHH
LRAILGLPIPKIEQLGPSASAVILASEKLDAPVFTGIAEALAAPDVDIRLFGKPTAHPYR
HHHHHCCCCCHHHHCCCCCCEEEEECCCCCCHHHHHHHHHHCCCCCEEEEECCCCCCHHH
RMGVALAKAENVQAAREKATDAASKIQISSQ
HHHHHHHHHHHHHHHHHHHHHHHHCEEECCC
>Mature Secondary Structure 
SKAIKLPQKLMLLGSGELGKEFVIAAQRLGNYVIAVDRYANAPAMQVADFCEVISMLSA
CCCHHCCHHHEEECCCCCCHHHHHHHHHHCCEEEEEECCCCCCHHHHHHHHHHHHHHCC
DDLEAVVTKYQPDFIIPEIEAIRTEKLQEFEDRGITVIPTAAATNYTMNRDRIRELAHEE
CHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCHHHHHHHHHHH
LGIRTAKYGYAITLEELIAVSDEIGFPNVVKPVMSSSGKGQSVVATKEEVEKAWNYAIAN
HCCEEECCCEEEEHHHHHHHHCCCCCCHHHHHHHCCCCCCCEEEECHHHHHHHHCCCEEC
SRGDSQKVIVEEFINFEIEITLLTIKQWDSPTIFCSPIGHRQERGDYQESWQPAGISEDK
CCCCCHHHHHHHHCCEEEEEEEEEEEECCCCEEEECCCCCCHHCCCHHHCCCCCCCCHHH
ILEAQAIAKKVTDALGGAGIFGVEFFITKDEVIFSELSPRPHDTGMVTLISQNLNEFELH
HHHHHHHHHHHHHHHCCCCEEEEEEEEECCHHHHHHCCCCCCCCCEEEEECCCCCHHHHH
LRAILGLPIPKIEQLGPSASAVILASEKLDAPVFTGIAEALAAPDVDIRLFGKPTAHPYR
HHHHHCCCCCHHHHCCCCCCEEEEECCCCCCHHHHHHHHHHCCCCCEEEEECCCCCCHHH
RMGVALAKAENVQAAREKATDAASKIQISSQ
HHHHHHHHHHHHHHHHHHHHHHHHCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Formate; 5'-Phospho-Ribosylglycinamide; acetate; ATP [C]

Specific reaction: Formate + ATP + 5'-Phospho-Ribosylglycinamide = 5'-Phosphoribosyl-N-Formylglycinamide + ADP + Pyrophosphate. acetate + ATP = acetylphosphate + ADP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA