Click here to switch to the map view.

The map label for this gene is cobA [H]

Identifier: 75909132

GI number: 75909132

Start: 3627414

End: 3628196

Strand: Direct

Name: cobA [H]

Synonym: Ava_2922

Alternate gene names: 75909132

Gene position: 3627414-3628196 (Clockwise)

Preceding gene: 75909131

Following gene: 75909134

Centisome position: 56.98

GC content: 46.23

Gene sequence:

>783_bases
ATGAACCGCACAGACAATGAGGGGCAAAAGAGTTTGGGTAAGGTCTATTTAGTAGGTGCGGGGCCAGGCGATCCAGGACT
GATGACGGTGAAAGGCAAAGGTATATTAGAATGTGCCGATGTGGTCATATATGATGCTTTAGTCAGTCCAGCAATTTTGG
CAATGATTAATCCCCAAGCAGAGCAAATTAACGCTGGTAAGCGCATGGGGAGACATTCCCTATTGCAGGACGAAACAACT
CAACTACTAATTGAACAAGCCCAAAATCATGCTGTTGTTGTGCGCTTAAAAGGTGGCGACCCCTTTATTTTCGGTCGTGG
TGGGGAAGAGATGGCTGATTTGATTGCGGCTGGGGTTCCGGTGGAGGTTGTACCAGGTATTACATCGGGAATTGCAGCGC
CAGCCTATGCGGGGATTCCCTTAACTCATCGCTTGTATAGTTCATCTGTGACTTTTGTAACTGGACATGAGTCGGCGGGT
AAGTATAGACCGAAAGTCAATTGGCAAGCGATCGCCCAAGGTTCGGAAACGATTGTCATTTATATGGGTATCCACAATCT
ACCTTACATTGTGGAACAGTTGAAGACGGCTGGGTTGAGTTTAGCAACACCAATTGCTTTAGTACGTTGGGGTACACGTC
CCGAACAAGAAGAATTAATTGGTGAGTTAGGGACAATTATAGAACTAGTGGAGAAAACCGGATTTGAAGCGCCAGCGATC
GCAGTCATTGGCTCTGTTGTCAATATGCACAGTATTTTATCTGTAGCAGGTCGTGTTCATTGA

Upstream 100 bases:

>100_bases
AAGCGCTAAAATTACAATTTCCTGGGGTAAGTTTATACTTGGCTGAACCTTTGGGAGTTAGTGGAGAATTAGCCGATTTA
ATTGGGGATTTGATTTACAC

Downstream 100 bases:

>100_bases
TTTGTCAATGTGTCTACATCTAATACCGTTTCTTGATGTAGCTTGCCTGGCGTAGCCATAGATGCACTTTATTCTGTACT
GTAGAGACGTTGCATACAAC

Product: uroporphyrin-III C-methyltransferase

Products: NA

Alternate protein names: Urogen III methylase; SUMT; Uroporphyrinogen III methylase; UROM [H]

Number of amino acids: Translated: 260; Mature: 260

Protein sequence:

>260_residues
MNRTDNEGQKSLGKVYLVGAGPGDPGLMTVKGKGILECADVVIYDALVSPAILAMINPQAEQINAGKRMGRHSLLQDETT
QLLIEQAQNHAVVVRLKGGDPFIFGRGGEEMADLIAAGVPVEVVPGITSGIAAPAYAGIPLTHRLYSSSVTFVTGHESAG
KYRPKVNWQAIAQGSETIVIYMGIHNLPYIVEQLKTAGLSLATPIALVRWGTRPEQEELIGELGTIIELVEKTGFEAPAI
AVIGSVVNMHSILSVAGRVH

Sequences:

>Translated_260_residues
MNRTDNEGQKSLGKVYLVGAGPGDPGLMTVKGKGILECADVVIYDALVSPAILAMINPQAEQINAGKRMGRHSLLQDETT
QLLIEQAQNHAVVVRLKGGDPFIFGRGGEEMADLIAAGVPVEVVPGITSGIAAPAYAGIPLTHRLYSSSVTFVTGHESAG
KYRPKVNWQAIAQGSETIVIYMGIHNLPYIVEQLKTAGLSLATPIALVRWGTRPEQEELIGELGTIIELVEKTGFEAPAI
AVIGSVVNMHSILSVAGRVH
>Mature_260_residues
MNRTDNEGQKSLGKVYLVGAGPGDPGLMTVKGKGILECADVVIYDALVSPAILAMINPQAEQINAGKRMGRHSLLQDETT
QLLIEQAQNHAVVVRLKGGDPFIFGRGGEEMADLIAAGVPVEVVPGITSGIAAPAYAGIPLTHRLYSSSVTFVTGHESAG
KYRPKVNWQAIAQGSETIVIYMGIHNLPYIVEQLKTAGLSLATPIALVRWGTRPEQEELIGELGTIIELVEKTGFEAPAI
AVIGSVVNMHSILSVAGRVH

Specific function: Catalyzes both methylations at C-2 and C-7 of uroporphyrinogen III leading to precorrin-1 and precorrin-2; their oxidative esterification gives respectively factor I octamethyl ester and sirohydrochlorin [H]

COG id: COG0007

COG function: function code H; Uroporphyrinogen-III methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the precorrin methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1789768, Length=249, Percent_Identity=43.7751004016064, Blast_Score=204, Evalue=7e-54,
Organism=Saccharomyces cerevisiae, GI6322922, Length=243, Percent_Identity=36.2139917695473, Blast_Score=124, Evalue=1e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000878
- InterPro:   IPR014777
- InterPro:   IPR014776
- InterPro:   IPR006366
- InterPro:   IPR003043 [H]

Pfam domain/function: PF00590 TP_methylase [H]

EC number: =2.1.1.107 [H]

Molecular weight: Translated: 27549; Mature: 27549

Theoretical pI: Translated: 6.16; Mature: 6.16

Prosite motif: PS00839 SUMT_1 ; PS00840 SUMT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNRTDNEGQKSLGKVYLVGAGPGDPGLMTVKGKGILECADVVIYDALVSPAILAMINPQA
CCCCCCCHHHCCCEEEEEECCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCH
EQINAGKRMGRHSLLQDETTQLLIEQAQNHAVVVRLKGGDPFIFGRGGEEMADLIAAGVP
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCEEEECCCCHHHHHHHHCCCC
VEVVPGITSGIAAPAYAGIPLTHRLYSSSVTFVTGHESAGKYRPKVNWQAIAQGSETIVI
EEECCCCHHCCCCCCCCCCCHHHHHHCCCEEEEECCCCCCCCCCCCCHHHEECCCCEEEE
YMGIHNLPYIVEQLKTAGLSLATPIALVRWGTRPEQEELIGELGTIIELVEKTGFEAPAI
EECCCCHHHHHHHHHHCCCCHHCHHHHEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCHH
AVIGSVVNMHSILSVAGRVH
HHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNRTDNEGQKSLGKVYLVGAGPGDPGLMTVKGKGILECADVVIYDALVSPAILAMINPQA
CCCCCCCHHHCCCEEEEEECCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCH
EQINAGKRMGRHSLLQDETTQLLIEQAQNHAVVVRLKGGDPFIFGRGGEEMADLIAAGVP
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCEEEECCCCHHHHHHHHCCCC
VEVVPGITSGIAAPAYAGIPLTHRLYSSSVTFVTGHESAGKYRPKVNWQAIAQGSETIVI
EEECCCCHHCCCCCCCCCCCHHHHHHCCCEEEEECCCCCCCCCCCCCHHHEECCCCEEEE
YMGIHNLPYIVEQLKTAGLSLATPIALVRWGTRPEQEELIGELGTIIELVEKTGFEAPAI
EECCCCHHHHHHHHHHCCCCHHCHHHHEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCHH
AVIGSVVNMHSILSVAGRVH
HHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231 [H]