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The map label for this gene is pmbA [H]

Identifier: 75908976

GI number: 75908976

Start: 3408685

End: 3410025

Strand: Direct

Name: pmbA [H]

Synonym: Ava_2764

Alternate gene names: 75908976

Gene position: 3408685-3410025 (Clockwise)

Preceding gene: 75908975

Following gene: 75908979

Centisome position: 53.55

GC content: 38.7

Gene sequence:

>1341_bases
ATGCCGAACATTAATGAAATCGCTACTTATGCCCAAGACAATGCTCAAAAGCTGGGCATCCAAAAATTCGATATTTATGG
CTCAACTGTAGATGAAACTAGTGTACAAGTTGACCAAGGTGAGCCAAAGCAAGTCAAAGCTTCAAATCGTTCTGGTGTAA
CTGTACGCGTCTGGAATGAAGACAATACCATAGGTATTACTAGCACCACAGATGTAGATCCCAAAGGGCTAGAATTAGCT
TTAAAAACGGCTTATGAAGCTAGTTTTTTTGGTGTCAAAGAAAATGTTCCTGATTTTAGTCCAGAGGCTACTGTTCCTCT
TGCTAATACGTTAAATGAGAAAGCGCCTCAAGCTCCTGTTTCGGAACTAATAGAAAAATTGCTAGTGGCTGAAAAAGAAC
TGCTGGCTGCTCATCCGGCTATCAAAGGTGTACCTTACAACGGCTTGGCACAAAGAGATATTGATCGATTTTATCTCAAT
AGCCAAGGTGCTTTAAGAACTGAGTCTGTTTCTTTGTCATCAGTTTATCTGTACAGCAAAACTGAAGAGGAAGGGAAAAA
ACCACGCAGTGCCGGTGCTTACAGAATCAACCGTAGCTTAGAAAATTTGGATATCAATGGATGTATCCAAGAAACTGCTG
AAAAAACTATTAGTCATCTCAATTACGACAAAATTAAAACTGGTAAGTATCGGGTGATTTTTTCACCGGAAGCCTTTTTA
AGTTTGCTAGGGGCTTTTTCTAATTTATTTAATGCCCAAAATATTCTTGATAAACAAAGCTTATCTACTCCCGATGATTT
AGGCAAGCAAATTGCTTCGCCTTTATTATCGGTTTATGATGATGCTTTGCACCCTGCGAATATAGGTGCAGAAAGTTTTG
ATGGCGAAGGTACACCTACTCGTCAGATTAGACTAATAGAAAATGGGATTTTAACAGGATTTCTTCATAGTGCTGGGACG
GCAAAAAGGTTGAATACTCAACCTACAGGTAATGCTAGTATTGGGGCGAAAGTTAGTGTTAGTCCTAACTTTTATCATGT
GTTTGCTTCTGGGAATTCTGAGGAGAAATTTAGCTTAGAAACAGCAGACAATGTAATTTTGATAGATGATTTACACGCTT
TGCACGCTGGTGTGAAATCTTTGCAAGGATCTTTTTCTCTGCCCTTTGATGGTTGGTTAATTAACAAAGGTGTCAAAACA
AGTATTGAATCAGCAACAGTTGCTGGTGATTTCTTAGAACTTTTGAAATCAATTATTTACGTAGAACCAGAAGTGGCTTT
AACACCTGGTGGTGTGGCTCCGAAAATTTGGGTGGAGGAATTATCAATTACTGGTGAATAA

Upstream 100 bases:

>100_bases
CTACATATATTTCTTAGGCGAAAAACGTAATTCATAATATCCAAAAATCCGAAATTTGATTTACTTGTCAAAATCCAAAA
TCTCAAATCAAAGATTGGCT

Downstream 100 bases:

>100_bases
ATTATGCCAAATTGACTGTTGGTAGGGTGCGTCGGTATGAATATTTTCTTGGTACAGCTAGTTTTTTTCGCAAGTAGCGC
ACCCTGCTGGAATGAAATTA

Product: peptidase U62, modulator of DNA gyrase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 446; Mature: 445

Protein sequence:

>446_residues
MPNINEIATYAQDNAQKLGIQKFDIYGSTVDETSVQVDQGEPKQVKASNRSGVTVRVWNEDNTIGITSTTDVDPKGLELA
LKTAYEASFFGVKENVPDFSPEATVPLANTLNEKAPQAPVSELIEKLLVAEKELLAAHPAIKGVPYNGLAQRDIDRFYLN
SQGALRTESVSLSSVYLYSKTEEEGKKPRSAGAYRINRSLENLDINGCIQETAEKTISHLNYDKIKTGKYRVIFSPEAFL
SLLGAFSNLFNAQNILDKQSLSTPDDLGKQIASPLLSVYDDALHPANIGAESFDGEGTPTRQIRLIENGILTGFLHSAGT
AKRLNTQPTGNASIGAKVSVSPNFYHVFASGNSEEKFSLETADNVILIDDLHALHAGVKSLQGSFSLPFDGWLINKGVKT
SIESATVAGDFLELLKSIIYVEPEVALTPGGVAPKIWVEELSITGE

Sequences:

>Translated_446_residues
MPNINEIATYAQDNAQKLGIQKFDIYGSTVDETSVQVDQGEPKQVKASNRSGVTVRVWNEDNTIGITSTTDVDPKGLELA
LKTAYEASFFGVKENVPDFSPEATVPLANTLNEKAPQAPVSELIEKLLVAEKELLAAHPAIKGVPYNGLAQRDIDRFYLN
SQGALRTESVSLSSVYLYSKTEEEGKKPRSAGAYRINRSLENLDINGCIQETAEKTISHLNYDKIKTGKYRVIFSPEAFL
SLLGAFSNLFNAQNILDKQSLSTPDDLGKQIASPLLSVYDDALHPANIGAESFDGEGTPTRQIRLIENGILTGFLHSAGT
AKRLNTQPTGNASIGAKVSVSPNFYHVFASGNSEEKFSLETADNVILIDDLHALHAGVKSLQGSFSLPFDGWLINKGVKT
SIESATVAGDFLELLKSIIYVEPEVALTPGGVAPKIWVEELSITGE
>Mature_445_residues
PNINEIATYAQDNAQKLGIQKFDIYGSTVDETSVQVDQGEPKQVKASNRSGVTVRVWNEDNTIGITSTTDVDPKGLELAL
KTAYEASFFGVKENVPDFSPEATVPLANTLNEKAPQAPVSELIEKLLVAEKELLAAHPAIKGVPYNGLAQRDIDRFYLNS
QGALRTESVSLSSVYLYSKTEEEGKKPRSAGAYRINRSLENLDINGCIQETAEKTISHLNYDKIKTGKYRVIFSPEAFLS
LLGAFSNLFNAQNILDKQSLSTPDDLGKQIASPLLSVYDDALHPANIGAESFDGEGTPTRQIRLIENGILTGFLHSAGTA
KRLNTQPTGNASIGAKVSVSPNFYHVFASGNSEEKFSLETADNVILIDDLHALHAGVKSLQGSFSLPFDGWLINKGVKTS
IESATVAGDFLELLKSIIYVEPEVALTPGGVAPKIWVEELSITGE

Specific function: May facilitate the secretion of the peptide antibiotic microcin B17 (MccB17) by completing its maturation. Suppresses the inhibitory activity of the carbon storage regulator (CsrA) [H]

COG id: COG0312

COG function: function code R; Predicted Zn-dependent proteases and their inactivated homologs

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase U62 family [H]

Homologues:

Organism=Escherichia coli, GI1790682, Length=423, Percent_Identity=24.113475177305, Blast_Score=90, Evalue=3e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002510 [H]

Pfam domain/function: PF01523 PmbA_TldD [H]

EC number: NA

Molecular weight: Translated: 48292; Mature: 48160

Theoretical pI: Translated: 4.70; Mature: 4.70

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
0.2 %Met     (Translated Protein)
0.4 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
0.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPNINEIATYAQDNAQKLGIQKFDIYGSTVDETSVQVDQGEPKQVKASNRSGVTVRVWNE
CCCHHHHHHHHHCCHHHCCCEEEEECCCCCCCCEEEECCCCCCEEECCCCCCCEEEEECC
DNTIGITSTTDVDPKGLELALKTAYEASFFGVKENVPDFSPEATVPLANTLNEKAPQAPV
CCEEEEEECCCCCCCCCEEEEHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHCCCCCCHH
SELIEKLLVAEKELLAAHPAIKGVPYNGLAQRDIDRFYLNSQGALRTESVSLSSVYLYSK
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHCCHHHEECCCCCEEECCEEEEEEEEEEC
TEEEGKKPRSAGAYRINRSLENLDINGCIQETAEKTISHLNYDKIKTGKYRVIFSPEAFL
CHHHCCCCCCCCCEEECCCHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCEEEEECHHHHH
SLLGAFSNLFNAQNILDKQSLSTPDDLGKQIASPLLSVYDDALHPANIGAESFDGEGTPT
HHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCC
RQIRLIENGILTGFLHSAGTAKRLNTQPTGNASIGAKVSVSPNFYHVFASGNSEEKFSLE
CEEEEECCCCHHHHHHCCCCCHHCCCCCCCCCCCCEEEEECCCEEEEEECCCCCCEEEEE
TADNVILIDDLHALHAGVKSLQGSFSLPFDGWLINKGVKTSIESATVAGDFLELLKSIIY
CCCCEEEEECHHHHHHHHHHHCCCCCCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHE
VEPEVALTPGGVAPKIWVEELSITGE
ECCCEEECCCCCCCCEEEEEEEECCC
>Mature Secondary Structure 
PNINEIATYAQDNAQKLGIQKFDIYGSTVDETSVQVDQGEPKQVKASNRSGVTVRVWNE
CCHHHHHHHHHCCHHHCCCEEEEECCCCCCCCEEEECCCCCCEEECCCCCCCEEEEECC
DNTIGITSTTDVDPKGLELALKTAYEASFFGVKENVPDFSPEATVPLANTLNEKAPQAPV
CCEEEEEECCCCCCCCCEEEEHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHCCCCCCHH
SELIEKLLVAEKELLAAHPAIKGVPYNGLAQRDIDRFYLNSQGALRTESVSLSSVYLYSK
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHCCHHHEECCCCCEEECCEEEEEEEEEEC
TEEEGKKPRSAGAYRINRSLENLDINGCIQETAEKTISHLNYDKIKTGKYRVIFSPEAFL
CHHHCCCCCCCCCEEECCCHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCEEEEECHHHHH
SLLGAFSNLFNAQNILDKQSLSTPDDLGKQIASPLLSVYDDALHPANIGAESFDGEGTPT
HHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCC
RQIRLIENGILTGFLHSAGTAKRLNTQPTGNASIGAKVSVSPNFYHVFASGNSEEKFSLE
CEEEEECCCCHHHHHHCCCCCHHCCCCCCCCCCCCEEEEECCCEEEEEECCCCCCEEEEE
TADNVILIDDLHALHAGVKSLQGSFSLPFDGWLINKGVKTSIESATVAGDFLELLKSIIY
CCCCEEEEECHHHHHHHHHHHCCCCCCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHE
VEPEVALTPGGVAPKIWVEELSITGE
ECCCEEECCCCCCCCEEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]