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The map label for this gene is gltA [H]

Identifier: 75908925

GI number: 75908925

Start: 3349921

End: 3351057

Strand: Direct

Name: gltA [H]

Synonym: Ava_2713

Alternate gene names: 75908925

Gene position: 3349921-3351057 (Clockwise)

Preceding gene: 75908924

Following gene: 75908926

Centisome position: 52.62

GC content: 46.35

Gene sequence:

>1137_bases
ATGATGGTGTGCGAATATAAGCCTGGTTTAGAAGGCATTCCCGCCGCCCAATCGAGTATCAGTTATGTAGATGGGCAAAA
GGGAATACTAGAATATCGTGGCATCCGGATTGAGGATTTAGCCCAGAAGAGTACTTTTCTGGAAACTGCTTATCTTTTAA
TCTGGGGTGAGTTGCCAACAAAAGAAGAATTGCAAGTATTTGAGGAGGAAGTCCGTCTTCATCGGCGGATTAAATACCGG
ATTCGGGATATGATGAAGTGCTTTCCCGAATCGGGTCATCCAATGGATGCACTCCAAGCCTCTGCTGCGGCTTTAGGCTT
GTTTTACTCCCGTCGAGATTTGCACAATCCTGTCTATATTCGGGATGCTGTAGTGCGCTTAATAGCTACTATTCCAACGA
TGGTAGCTGCATTCCAGTTGATGCGGAAAGGCAATGACCCCGTTAAACCCCGCGATGATTTAGATTATTCCGCCAATTTT
CTCTACATGCTCAACGAGAAAGAACCGGATGCTTTGGCGGCAAAAATCTTTGATATCTGCTTGATTCTCCATGTCGAGCA
TACGATGAATGCTTCTACCTTCAGTGCTAGGGTAACAGCTTCAACCTTGACAGACCCCTATGCGGTGGTTGCTAGCGCTG
TGGGAACCTTGGGAGGGCCTTTACACGGTGGAGCCAATGAAGAAGTAATTCAGATGTTGGAAGAAATCGGTTCCGTGGAG
AATGTGCGTTCTTATGTCGAAGAGAGGTTGCAACGGAAAGACAAGCTCATGGGCTTTGGACATCGTGTTTACAAGGTGAA
AGACCCACGGGCAACAATTTTGCAAGGTCTCGCAGAACAGTTGTTTGCCAAGTTCGGCGCGGATAAGTATTACGACATCG
CCCAAGAAATGGAACGGGTAGTCGAAGAGAAGCTTGGTCATAAAGGGATTTATCCCAATGTTGACTTTTACTCAGGTCTA
GTGTATAGGAAGATGGGTATTCCTACAGACTTGTTTACACCAATCTTTGCGATCGCTCGTGTGGCTGGTTGGTTAGCCCA
CTGGAAAGAACAACTTGAAGAAAACCGCATCTTCCGTCCTACCCAGGTTTATAACGGCAAACACAGTGTTACTTACACCC
CCATTGACCAACGTTAA

Upstream 100 bases:

>100_bases
GTTCTGCCAAGAATTGTTGTGAGCTAGGCTAGTGTTATGGCGGCAATAATTTCTGGTAAGTTAGCTTGATCAGATATTTC
ACTAAGCAAATGGTGTTGCC

Downstream 100 bases:

>100_bases
AAGCTGGGGAATCGGTAAATTAATAGCCAGCTGATAACCGTTACCAAAATGGAAGCGGCGTTTTTCTGATGCTTTTGTGA
AGCACTGGGAATTAGGGATA

Product: citrate synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 378; Mature: 378

Protein sequence:

>378_residues
MMVCEYKPGLEGIPAAQSSISYVDGQKGILEYRGIRIEDLAQKSTFLETAYLLIWGELPTKEELQVFEEEVRLHRRIKYR
IRDMMKCFPESGHPMDALQASAAALGLFYSRRDLHNPVYIRDAVVRLIATIPTMVAAFQLMRKGNDPVKPRDDLDYSANF
LYMLNEKEPDALAAKIFDICLILHVEHTMNASTFSARVTASTLTDPYAVVASAVGTLGGPLHGGANEEVIQMLEEIGSVE
NVRSYVEERLQRKDKLMGFGHRVYKVKDPRATILQGLAEQLFAKFGADKYYDIAQEMERVVEEKLGHKGIYPNVDFYSGL
VYRKMGIPTDLFTPIFAIARVAGWLAHWKEQLEENRIFRPTQVYNGKHSVTYTPIDQR

Sequences:

>Translated_378_residues
MMVCEYKPGLEGIPAAQSSISYVDGQKGILEYRGIRIEDLAQKSTFLETAYLLIWGELPTKEELQVFEEEVRLHRRIKYR
IRDMMKCFPESGHPMDALQASAAALGLFYSRRDLHNPVYIRDAVVRLIATIPTMVAAFQLMRKGNDPVKPRDDLDYSANF
LYMLNEKEPDALAAKIFDICLILHVEHTMNASTFSARVTASTLTDPYAVVASAVGTLGGPLHGGANEEVIQMLEEIGSVE
NVRSYVEERLQRKDKLMGFGHRVYKVKDPRATILQGLAEQLFAKFGADKYYDIAQEMERVVEEKLGHKGIYPNVDFYSGL
VYRKMGIPTDLFTPIFAIARVAGWLAHWKEQLEENRIFRPTQVYNGKHSVTYTPIDQR
>Mature_378_residues
MMVCEYKPGLEGIPAAQSSISYVDGQKGILEYRGIRIEDLAQKSTFLETAYLLIWGELPTKEELQVFEEEVRLHRRIKYR
IRDMMKCFPESGHPMDALQASAAALGLFYSRRDLHNPVYIRDAVVRLIATIPTMVAAFQLMRKGNDPVKPRDDLDYSANF
LYMLNEKEPDALAAKIFDICLILHVEHTMNASTFSARVTASTLTDPYAVVASAVGTLGGPLHGGANEEVIQMLEEIGSVE
NVRSYVEERLQRKDKLMGFGHRVYKVKDPRATILQGLAEQLFAKFGADKYYDIAQEMERVVEEKLGHKGIYPNVDFYSGL
VYRKMGIPTDLFTPIFAIARVAGWLAHWKEQLEENRIFRPTQVYNGKHSVTYTPIDQR

Specific function: Tricarboxylic acid cycle. [C]

COG id: COG0372

COG function: function code C; Citrate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the citrate synthase family [H]

Homologues:

Organism=Homo sapiens, GI38327625, Length=380, Percent_Identity=27.8947368421053, Blast_Score=119, Evalue=4e-27,
Organism=Escherichia coli, GI1786939, Length=376, Percent_Identity=38.8297872340425, Blast_Score=280, Evalue=1e-76,
Organism=Escherichia coli, GI1786527, Length=370, Percent_Identity=34.8648648648649, Blast_Score=253, Evalue=2e-68,
Organism=Caenorhabditis elegans, GI17555174, Length=376, Percent_Identity=26.8617021276596, Blast_Score=110, Evalue=2e-24,
Organism=Saccharomyces cerevisiae, GI6319850, Length=371, Percent_Identity=25.6064690026954, Blast_Score=106, Evalue=6e-24,
Organism=Saccharomyces cerevisiae, GI6325257, Length=386, Percent_Identity=25.1295336787565, Blast_Score=102, Evalue=1e-22,
Organism=Saccharomyces cerevisiae, GI6324328, Length=367, Percent_Identity=24.5231607629428, Blast_Score=99, Evalue=1e-21,
Organism=Drosophila melanogaster, GI24640126, Length=371, Percent_Identity=27.2237196765499, Blast_Score=120, Evalue=1e-27,
Organism=Drosophila melanogaster, GI24640124, Length=371, Percent_Identity=27.2237196765499, Blast_Score=120, Evalue=1e-27,
Organism=Drosophila melanogaster, GI21356863, Length=380, Percent_Identity=27.3684210526316, Blast_Score=112, Evalue=4e-25,

Paralogues:

None

Copy number: 624 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 2,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011278
- InterPro:   IPR016142
- InterPro:   IPR016143
- InterPro:   IPR002020
- InterPro:   IPR016141
- InterPro:   IPR019810 [H]

Pfam domain/function: PF00285 Citrate_synt [H]

EC number: =2.3.3.1 [H]

Molecular weight: Translated: 42963; Mature: 42963

Theoretical pI: Translated: 6.68; Mature: 6.68

Prosite motif: PS00480 CITRATE_SYNTHASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMVCEYKPGLEGIPAAQSSISYVDGQKGILEYRGIRIEDLAQKSTFLETAYLLIWGELPT
CEEECCCCCCCCCCCCHHHCHHCCCCCCHHHHCCCCHHHHHHHHHHHHHHHHEEECCCCC
KEELQVFEEEVRLHRRIKYRIRDMMKCFPESGHPMDALQASAAALGLFYSRRDLHNPVYI
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHH
RDAVVRLIATIPTMVAAFQLMRKGNDPVKPRDDLDYSANFLYMLNEKEPDALAAKIFDIC
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHH
LILHVEHTMNASTFSARVTASTLTDPYAVVASAVGTLGGPLHGGANEEVIQMLEEIGSVE
HHHHHHHHCCCCHHHHEEEHHHCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCHH
NVRSYVEERLQRKDKLMGFGHRVYKVKDPRATILQGLAEQLFAKFGADKYYDIAQEMERV
HHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
VEEKLGHKGIYPNVDFYSGLVYRKMGIPTDLFTPIFAIARVAGWLAHWKEQLEENRIFRP
HHHHHCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
TQVYNGKHSVTYTPIDQR
HHHCCCCCCEEECCCCCC
>Mature Secondary Structure
MMVCEYKPGLEGIPAAQSSISYVDGQKGILEYRGIRIEDLAQKSTFLETAYLLIWGELPT
CEEECCCCCCCCCCCCHHHCHHCCCCCCHHHHCCCCHHHHHHHHHHHHHHHHEEECCCCC
KEELQVFEEEVRLHRRIKYRIRDMMKCFPESGHPMDALQASAAALGLFYSRRDLHNPVYI
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHH
RDAVVRLIATIPTMVAAFQLMRKGNDPVKPRDDLDYSANFLYMLNEKEPDALAAKIFDIC
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHH
LILHVEHTMNASTFSARVTASTLTDPYAVVASAVGTLGGPLHGGANEEVIQMLEEIGSVE
HHHHHHHHCCCCHHHHEEEHHHCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCHH
NVRSYVEERLQRKDKLMGFGHRVYKVKDPRATILQGLAEQLFAKFGADKYYDIAQEMERV
HHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
VEEKLGHKGIYPNVDFYSGLVYRKMGIPTDLFTPIFAIARVAGWLAHWKEQLEENRIFRP
HHHHHCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
TQVYNGKHSVTYTPIDQR
HHHCCCCCCEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231 [H]