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The map label for this gene is folD

Identifier: 75908915

GI number: 75908915

Start: 3340436

End: 3341335

Strand: Direct

Name: folD

Synonym: Ava_2703

Alternate gene names: 75908915

Gene position: 3340436-3341335 (Clockwise)

Preceding gene: 75908911

Following gene: 75908916

Centisome position: 52.48

GC content: 44.56

Gene sequence:

>900_bases
ATGGAAACAAAAACTGCCAAAATTCTTGATGGTAAAACTTTAGCTGAGAAAATTCAAAAAGAACTGACTGCCCAAATTAT
AGAAGTACAGGCCAAAATTGGTCGTCCTCCTGGGTTAGCAGTATTGATGGTTGGTGATAACCCAGCCTCAGCAGCCTATG
TACGGAACAAGGAAAAATCCTGTGCCAAAGTGGGTATTGCTTCTTTTGGTAAGCATTTTCCCCAAGAAACTAGCCAAAAA
GAGTTAGAGGATGTGATTGCTGCACTTAACCAAGATGAACAGGTAGATGGCATTCTTGTACAACTACCCTTACCTGAGCA
TCTGGATGCAGTAAAGTTGCTGCATCAAATAGAACCGGATAAAGATGCTGATGGACTCCACCCAGTGAATTTAGGGCGTT
TGGTGCGTGGGGAAAAAGGTTTACGCAGTTGCACCCCAGCTGGAGTAATGCGGCTATTGGCAGAATATGAAATTTCTTTG
CGAGGAAAGCAAGCTGTGGTGGTGGGACGGAGTATTTTGGTAGGGAAGCCTATGGCTTTGATGCTCCTAGAAGCTGATGC
CACCGTGACTATTGCCCACTCGCGATCGCAAGACCTAAAATCCATCACCCAAAATGCTGATATTCTCGTTGCTGCTGCCG
GTTTGCCAGGATTAATTACTGCTGACATGGTGAAACCAGGTGCTGTTGTGGTAGATGTGGGGATAAATCGCGTGACTGAT
GCTAATGGCAAAAGTCGCTTAGTAGGCGATATTAATTTTGCATCAATTGCTGGTGTGGCAGAATATATCACCCCAGTTCC
CGGTGGTATTGGCCCTATGACTGTGGCTTTGTTATTACAAAATACAGTCACCAGCTATTTACAAACAGCAAAAGAAAGCG
GAGCCTTGGATGTTAAATGA

Upstream 100 bases:

>100_bases
GTGGTAGAAAAGACGCTACGGCATCAGATGGCAACGCCATCCATCATAATTACTGCGGACAACTGAAATTTGCACCAAAG
CGCATCTTCTGGGAATTCTC

Downstream 100 bases:

>100_bases
AAAATTACGAATCGTAACATAAATCATCATTTATCATCCATCTTTGATGATTACTGAAGTATGAGTTAATCTTTCAGCTC
CAGAGCATCTTAAAATTGTG

Product: bifunctional 5,10-methylene-tetrahydrofolate dehydrogenase/ 5,10-methylene-tetrahydrofolate cyclohydrolase

Products: NA

Alternate protein names: Methylenetetrahydrofolate dehydrogenase; Methenyltetrahydrofolate cyclohydrolase

Number of amino acids: Translated: 299; Mature: 299

Protein sequence:

>299_residues
METKTAKILDGKTLAEKIQKELTAQIIEVQAKIGRPPGLAVLMVGDNPASAAYVRNKEKSCAKVGIASFGKHFPQETSQK
ELEDVIAALNQDEQVDGILVQLPLPEHLDAVKLLHQIEPDKDADGLHPVNLGRLVRGEKGLRSCTPAGVMRLLAEYEISL
RGKQAVVVGRSILVGKPMALMLLEADATVTIAHSRSQDLKSITQNADILVAAAGLPGLITADMVKPGAVVVDVGINRVTD
ANGKSRLVGDINFASIAGVAEYITPVPGGIGPMTVALLLQNTVTSYLQTAKESGALDVK

Sequences:

>Translated_299_residues
METKTAKILDGKTLAEKIQKELTAQIIEVQAKIGRPPGLAVLMVGDNPASAAYVRNKEKSCAKVGIASFGKHFPQETSQK
ELEDVIAALNQDEQVDGILVQLPLPEHLDAVKLLHQIEPDKDADGLHPVNLGRLVRGEKGLRSCTPAGVMRLLAEYEISL
RGKQAVVVGRSILVGKPMALMLLEADATVTIAHSRSQDLKSITQNADILVAAAGLPGLITADMVKPGAVVVDVGINRVTD
ANGKSRLVGDINFASIAGVAEYITPVPGGIGPMTVALLLQNTVTSYLQTAKESGALDVK
>Mature_299_residues
METKTAKILDGKTLAEKIQKELTAQIIEVQAKIGRPPGLAVLMVGDNPASAAYVRNKEKSCAKVGIASFGKHFPQETSQK
ELEDVIAALNQDEQVDGILVQLPLPEHLDAVKLLHQIEPDKDADGLHPVNLGRLVRGEKGLRSCTPAGVMRLLAEYEISL
RGKQAVVVGRSILVGKPMALMLLEADATVTIAHSRSQDLKSITQNADILVAAAGLPGLITADMVKPGAVVVDVGINRVTD
ANGKSRLVGDINFASIAGVAEYITPVPGGIGPMTVALLLQNTVTSYLQTAKESGALDVK

Specific function: Catalyzes the oxidation of 5,10- methylenetetrahydrofolate to 5,10-methenyltetrahydrofolate and then the hydrolysis of 5,10-methenyltetrahydrofolate to 10- formyltetrahydrofolate

COG id: COG0190

COG function: function code H; 5,10-methylene-tetrahydrofolate dehydrogenase/Methenyl tetrahydrofolate cyclohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tetrahydrofolate dehydrogenase/cyclohydrolase family

Homologues:

Organism=Homo sapiens, GI222418558, Length=290, Percent_Identity=47.9310344827586, Blast_Score=243, Evalue=2e-64,
Organism=Homo sapiens, GI94721354, Length=289, Percent_Identity=46.0207612456747, Blast_Score=240, Evalue=1e-63,
Organism=Homo sapiens, GI222136639, Length=288, Percent_Identity=40.625, Blast_Score=213, Evalue=2e-55,
Organism=Homo sapiens, GI36796743, Length=199, Percent_Identity=27.1356783919598, Blast_Score=74, Evalue=2e-13,
Organism=Escherichia coli, GI1786741, Length=286, Percent_Identity=49.6503496503497, Blast_Score=276, Evalue=2e-75,
Organism=Caenorhabditis elegans, GI17568735, Length=252, Percent_Identity=40.8730158730159, Blast_Score=184, Evalue=3e-47,
Organism=Saccharomyces cerevisiae, GI6319558, Length=299, Percent_Identity=41.8060200668896, Blast_Score=229, Evalue=5e-61,
Organism=Saccharomyces cerevisiae, GI6321643, Length=300, Percent_Identity=38.3333333333333, Blast_Score=218, Evalue=1e-57,
Organism=Saccharomyces cerevisiae, GI6322933, Length=312, Percent_Identity=26.2820512820513, Blast_Score=70, Evalue=6e-13,
Organism=Drosophila melanogaster, GI17136816, Length=294, Percent_Identity=45.9183673469388, Blast_Score=247, Evalue=7e-66,
Organism=Drosophila melanogaster, GI17136818, Length=291, Percent_Identity=46.3917525773196, Blast_Score=247, Evalue=8e-66,
Organism=Drosophila melanogaster, GI24645718, Length=286, Percent_Identity=41.958041958042, Blast_Score=218, Evalue=3e-57,
Organism=Drosophila melanogaster, GI17137370, Length=286, Percent_Identity=41.958041958042, Blast_Score=218, Evalue=3e-57,
Organism=Drosophila melanogaster, GI62472483, Length=286, Percent_Identity=41.958041958042, Blast_Score=218, Evalue=3e-57,
Organism=Drosophila melanogaster, GI45551871, Length=286, Percent_Identity=41.958041958042, Blast_Score=218, Evalue=3e-57,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): FOLD_ANAVT (Q3M9M0)

Other databases:

- EMBL:   CP000117
- RefSeq:   YP_323211.1
- HSSP:   O50385
- ProteinModelPortal:   Q3M9M0
- SMR:   Q3M9M0
- STRING:   Q3M9M0
- GeneID:   3682201
- GenomeReviews:   CP000117_GR
- KEGG:   ava:Ava_2703
- NMPDR:   fig|240292.3.peg.1041
- eggNOG:   COG0190
- HOGENOM:   HBG328751
- OMA:   DVVYETA
- PhylomeDB:   Q3M9M0
- ProtClustDB:   PRK14186
- BioCyc:   AVAR240292:AVA_2703-MONOMER
- GO:   GO:0005488
- HAMAP:   MF_01576
- InterPro:   IPR016040
- InterPro:   IPR000672
- InterPro:   IPR020630
- InterPro:   IPR020867
- InterPro:   IPR020631
- Gene3D:   G3DSA:3.40.50.720
- PRINTS:   PR00085

Pfam domain/function: PF00763 THF_DHG_CYH; PF02882 THF_DHG_CYH_C

EC number: =1.5.1.5; =3.5.4.9

Molecular weight: Translated: 31583; Mature: 31583

Theoretical pI: Translated: 6.69; Mature: 6.69

Prosite motif: PS00766 THF_DHG_CYH_1; PS00767 THF_DHG_CYH_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
METKTAKILDGKTLAEKIQKELTAQIIEVQAKIGRPPGLAVLMVGDNPASAAYVRNKEKS
CCCCCCCEECCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHCCHHH
CAKVGIASFGKHFPQETSQKELEDVIAALNQDEQVDGILVQLPLPEHLDAVKLLHQIEPD
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCEEEEECCCHHHHHHHHHHHCCCC
KDADGLHPVNLGRLVRGEKGLRSCTPAGVMRLLAEYEISLRGKQAVVVGRSILVGKPMAL
CCCCCCCCCCHHHHHHCCCCHHCCCHHHHHHHHHHHHHHCCCCEEEEECCHHECCCCEEE
MLLEADATVTIAHSRSQDLKSITQNADILVAAAGLPGLITADMVKPGAVVVDVGINRVTD
EEEECCCEEEEEECCCHHHHHHHCCCCEEEEECCCCCCEEHHHCCCCCEEEECCCCEEEC
ANGKSRLVGDINFASIAGVAEYITPVPGGIGPMTVALLLQNTVTSYLQTAKESGALDVK
CCCCCEEEECCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
METKTAKILDGKTLAEKIQKELTAQIIEVQAKIGRPPGLAVLMVGDNPASAAYVRNKEKS
CCCCCCCEECCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHCCHHH
CAKVGIASFGKHFPQETSQKELEDVIAALNQDEQVDGILVQLPLPEHLDAVKLLHQIEPD
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCEEEEECCCHHHHHHHHHHHCCCC
KDADGLHPVNLGRLVRGEKGLRSCTPAGVMRLLAEYEISLRGKQAVVVGRSILVGKPMAL
CCCCCCCCCCHHHHHHCCCCHHCCCHHHHHHHHHHHHHHCCCCEEEEECCHHECCCCEEE
MLLEADATVTIAHSRSQDLKSITQNADILVAAAGLPGLITADMVKPGAVVVDVGINRVTD
EEEECCCEEEEEECCCHHHHHHHCCCCEEEEECCCCCCEEHHHCCCCCEEEECCCCEEEC
ANGKSRLVGDINFASIAGVAEYITPVPGGIGPMTVALLLQNTVTSYLQTAKESGALDVK
CCCCCEEEECCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA