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The map label for this gene is rph

Identifier: 75908883

GI number: 75908883

Start: 3300639

End: 3301382

Strand: Direct

Name: rph

Synonym: Ava_2671

Alternate gene names: 75908883

Gene position: 3300639-3301382 (Clockwise)

Preceding gene: 75908882

Following gene: 75908885

Centisome position: 51.85

GC content: 43.55

Gene sequence:

>744_bases
ATGGTTTGGCAGCGTCCCGACGGTAGAAAACCCTATGAACTCCGTCCTATCAATTTTCACACTAAATTTACTCGTTTTGC
CCCTGGCTCGGTTCTGACAATATGCGGTGAGACTAAGGTATTGTGTACTGTTAGCGTTGCGGAAAGTGTACCCAAGTTTC
TTGCAGGTAGTGGCAAAGGTTGGTTAACGGCTGAGTATCGGATGCTACCATCAGCTACACAACAACGGCATGAAAGAGAA
TTATTGAAGTTATCTGGACGGACGCAAGAAATTCAACGTTTGATTGGGCGTAGTTTACGAGCAGCGATAGATTTTGAGGC
GTTAGGAGAACGGACTTTAACTGTAGACGCAGATGTGTTACAAGCTGATGCAGGTACGAGAACGGCAGCAATTACGGGCG
GATTTGTAGCATTAGCTGAGGCTATTTCTCAATTATTGCAGCGAGGGGTATTAGAGCGATCGCCTTTATGTGGACAAGTC
GCCGCCGTTTCTGTGGGTTTATTAGAACAAGAAGCATATTTAGATTTGAACTATATAGAAGATGTAGCCGCAACGGTAGA
TTTCAACGTTGTGATGAATAAAAACTTAGGAATTATTGAAGTTCAGGGAACAGCAGAAGAAGGTAGTTTTAGCCGTACCC
AATTGAATCAGCTATTAGATTGCGCCGAAACAGGAATTCAGCAACTATTAATTGCCCAACAGAAAGCAATTACTGATTGG
GATGAGTTGTTTGTGGGCAAGTAG

Upstream 100 bases:

>100_bases
TCTGGTTATCAGATGCTGATCCCGCATAGTACTTAATGTTTTGCACCACTAAGGTAACTTTAAAGGGGTATTCTACAATT
AAAAGTTAGAGGTGACTCTC

Downstream 100 bases:

>100_bases
AGAGTGGGGAGTTAAAGGTTTGTCTTTTTCTTCTCCCTTATTTCTTCTGGAGAATATCTAATAAGTCTGTTGTTTGCGAT
CGCTCTACCAAGGTATTTGC

Product: ribonuclease PH

Products: NA

Alternate protein names: RNase PH; tRNA nucleotidyltransferase

Number of amino acids: Translated: 247; Mature: 247

Protein sequence:

>247_residues
MVWQRPDGRKPYELRPINFHTKFTRFAPGSVLTICGETKVLCTVSVAESVPKFLAGSGKGWLTAEYRMLPSATQQRHERE
LLKLSGRTQEIQRLIGRSLRAAIDFEALGERTLTVDADVLQADAGTRTAAITGGFVALAEAISQLLQRGVLERSPLCGQV
AAVSVGLLEQEAYLDLNYIEDVAATVDFNVVMNKNLGIIEVQGTAEEGSFSRTQLNQLLDCAETGIQQLLIAQQKAITDW
DELFVGK

Sequences:

>Translated_247_residues
MVWQRPDGRKPYELRPINFHTKFTRFAPGSVLTICGETKVLCTVSVAESVPKFLAGSGKGWLTAEYRMLPSATQQRHERE
LLKLSGRTQEIQRLIGRSLRAAIDFEALGERTLTVDADVLQADAGTRTAAITGGFVALAEAISQLLQRGVLERSPLCGQV
AAVSVGLLEQEAYLDLNYIEDVAATVDFNVVMNKNLGIIEVQGTAEEGSFSRTQLNQLLDCAETGIQQLLIAQQKAITDW
DELFVGK
>Mature_247_residues
MVWQRPDGRKPYELRPINFHTKFTRFAPGSVLTICGETKVLCTVSVAESVPKFLAGSGKGWLTAEYRMLPSATQQRHERE
LLKLSGRTQEIQRLIGRSLRAAIDFEALGERTLTVDADVLQADAGTRTAAITGGFVALAEAISQLLQRGVLERSPLCGQV
AAVSVGLLEQEAYLDLNYIEDVAATVDFNVVMNKNLGIIEVQGTAEEGSFSRTQLNQLLDCAETGIQQLLIAQQKAITDW
DELFVGK

Specific function: Phosphorolytic exoribonuclease that removes nucleotide residues following the -CCA terminus of tRNA and adds nucleotides to the ends of RNA molecules by using nucleoside diphosphates as substrates

COG id: COG0689

COG function: function code J; RNase PH

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNase PH family

Homologues:

Organism=Escherichia coli, GI157672248, Length=210, Percent_Identity=55.7142857142857, Blast_Score=230, Evalue=6e-62,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RNPH_ANAVT (Q3M9Q2)

Other databases:

- EMBL:   CP000117
- RefSeq:   YP_323179.1
- HSSP:   Q9UXC2
- ProteinModelPortal:   Q3M9Q2
- STRING:   Q3M9Q2
- GeneID:   3682032
- GenomeReviews:   CP000117_GR
- KEGG:   ava:Ava_2671
- NMPDR:   fig|240292.3.peg.945
- eggNOG:   COG0689
- HOGENOM:   HBG737187
- OMA:   MLPRATG
- PhylomeDB:   Q3M9Q2
- ProtClustDB:   PRK00173
- BioCyc:   AVAR240292:AVA_2671-MONOMER
- HAMAP:   MF_00564
- InterPro:   IPR001247
- InterPro:   IPR015847
- InterPro:   IPR020568
- InterPro:   IPR002381
- InterPro:   IPR018336
- TIGRFAMs:   TIGR01966

Pfam domain/function: PF01138 RNase_PH; PF03725 RNase_PH_C; SSF55666 3_ExoRNase; SSF54211 Ribosomal_S5_D2-typ_fold

EC number: =2.7.7.56

Molecular weight: Translated: 27081; Mature: 27081

Theoretical pI: Translated: 4.99; Mature: 4.99

Prosite motif: PS01277 RIBONUCLEASE_PH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVWQRPDGRKPYELRPINFHTKFTRFAPGSVLTICGETKVLCTVSVAESVPKFLAGSGKG
CCCCCCCCCCCCEEEECCCCCCEEEECCCCEEEEECCCEEEEEEHHHHHHHHHHHCCCCC
WLTAEYRMLPSATQQRHERELLKLSGRTQEIQRLIGRSLRAAIDFEALGERTLTVDADVL
EEEEEEEECCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCHHHHCCEEEEEEHHHH
QADAGTRTAAITGGFVALAEAISQLLQRGVLERSPLCGQVAAVSVGLLEQEAYLDLNYIE
HCCCCCCEEECCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCHHHHH
DVAATVDFNVVMNKNLGIIEVQGTAEEGSFSRTQLNQLLDCAETGIQQLLIAQQKAITDW
HHHHHEEEEEEEECCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCH
DELFVGK
HHHHCCC
>Mature Secondary Structure
MVWQRPDGRKPYELRPINFHTKFTRFAPGSVLTICGETKVLCTVSVAESVPKFLAGSGKG
CCCCCCCCCCCCEEEECCCCCCEEEECCCCEEEEECCCEEEEEEHHHHHHHHHHHCCCCC
WLTAEYRMLPSATQQRHERELLKLSGRTQEIQRLIGRSLRAAIDFEALGERTLTVDADVL
EEEEEEEECCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCHHHHCCEEEEEEHHHH
QADAGTRTAAITGGFVALAEAISQLLQRGVLERSPLCGQVAAVSVGLLEQEAYLDLNYIE
HCCCCCCEEECCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCHHHHH
DVAATVDFNVVMNKNLGIIEVQGTAEEGSFSRTQLNQLLDCAETGIQQLLIAQQKAITDW
HHHHHEEEEEEEECCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCH
DELFVGK
HHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA