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The map label for this gene is ddh [H]

Identifier: 75908872

GI number: 75908872

Start: 3289277

End: 3290302

Strand: Direct

Name: ddh [H]

Synonym: Ava_2660

Alternate gene names: 75908872

Gene position: 3289277-3290302 (Clockwise)

Preceding gene: 75908871

Following gene: 75908876

Centisome position: 51.67

GC content: 41.81

Gene sequence:

>1026_bases
ATGAAAGTAGCAGTCTTCAGTACAAAAGCCTATGATCGGCAGTTTTTAGAAGCTGCAAATGCTCCAAAGCAACATGATTT
GGTGTTTTTTGAACCCCGTCTCAATCAAGATACCGCCATTTTAGCCGCCGGATTTCCGGCGGTTTGTGTATTTGTACATG
ATCAAGTTGATGCAGCTACTTTAGAAATTCTTGCTTCACGAGGAACTCGCTTGATTGTCCTCCGTTGTGCAGGTTTCAAT
AATGTAGATTTAAAAGCTGCTAATAAGTTGGGTGTTAATGTAGTTCGTGTTCCCGCCTATTCACCCTATGGTGTAGCCGA
ACACGCAGTAGGTTTAATTTTGAGCCTCAATCGCAAAATCCACCGCGCTTATAATCGCGTCCGTGAAGGTAATTTTGCTT
TAGATGGACTTTTAGGTTTTAACATCAACGGACGTACGGTGGGAATTATCGGTACTGGTAAAATCGGTTTGATTTTGGGA
CAAATCATGAAAGGGTTTGGCTGTCGTCTACTCGCCTATGATGTGTACCACAACCCAGAAATGTTAGCATTAGGGGGGGA
ATACGTAGAATTACCGGAACTATTTGCCAATTCTGATATTATTTCTCTCCATTGCCCCCTGATGCCGCAAACTCATCACT
TAATCAACGCGGAAGCAATCGAGCAGGTAAAGCCAGGGGTAATGTTAATCAATACCAGCAGAGGTGCGCTCATTCATACC
CAAGCTGTAATTGAAGGACTCAAGACTGGCAAAATCGGTTCTTTGGGTGTAGATGTTTATGAGCAGGAATCAGAATTGTT
TTTTGAGGATTTATCAGGGGAAATCATTCAAGATGATATCTTCCAAAGACTGACAACATTTCCCAATGTACTTATCACAG
GACACCAAGCATTTTTTACAGAAGATGCTTTACGCAACATAGCAGAGACAACCTTAAACAACATTGCTGATATTGAGCAA
GGTCGTTCTTGTCCCAATGAAATCCGTTATCAACCAGAAGTAGAAGCTAAGGTTCTAGTTAGTTGA

Upstream 100 bases:

>100_bases
ATAATGGAGATTTTCAGCTAGCCCTTAGTGGACATCAACTGTTCAAACACCAAGCTAATATGTATGTGAAAAATATCCGG
CATACAGCCTGAGGTTTGGC

Downstream 100 bases:

>100_bases
GTATAGGGGTTTAGGGGTTTAGGGGTATAAATTTTGAAAAACCTTACACCCTTACGCCCTTACACCCATTCTCAACAGAC
TACCTGAGTGCGTAAGTTCT

Product: D-lactate dehydrogenase

Products: pyruvate; NADH; H+

Alternate protein names: NA

Number of amino acids: Translated: 341; Mature: 341

Protein sequence:

>341_residues
MKVAVFSTKAYDRQFLEAANAPKQHDLVFFEPRLNQDTAILAAGFPAVCVFVHDQVDAATLEILASRGTRLIVLRCAGFN
NVDLKAANKLGVNVVRVPAYSPYGVAEHAVGLILSLNRKIHRAYNRVREGNFALDGLLGFNINGRTVGIIGTGKIGLILG
QIMKGFGCRLLAYDVYHNPEMLALGGEYVELPELFANSDIISLHCPLMPQTHHLINAEAIEQVKPGVMLINTSRGALIHT
QAVIEGLKTGKIGSLGVDVYEQESELFFEDLSGEIIQDDIFQRLTTFPNVLITGHQAFFTEDALRNIAETTLNNIADIEQ
GRSCPNEIRYQPEVEAKVLVS

Sequences:

>Translated_341_residues
MKVAVFSTKAYDRQFLEAANAPKQHDLVFFEPRLNQDTAILAAGFPAVCVFVHDQVDAATLEILASRGTRLIVLRCAGFN
NVDLKAANKLGVNVVRVPAYSPYGVAEHAVGLILSLNRKIHRAYNRVREGNFALDGLLGFNINGRTVGIIGTGKIGLILG
QIMKGFGCRLLAYDVYHNPEMLALGGEYVELPELFANSDIISLHCPLMPQTHHLINAEAIEQVKPGVMLINTSRGALIHT
QAVIEGLKTGKIGSLGVDVYEQESELFFEDLSGEIIQDDIFQRLTTFPNVLITGHQAFFTEDALRNIAETTLNNIADIEQ
GRSCPNEIRYQPEVEAKVLVS
>Mature_341_residues
MKVAVFSTKAYDRQFLEAANAPKQHDLVFFEPRLNQDTAILAAGFPAVCVFVHDQVDAATLEILASRGTRLIVLRCAGFN
NVDLKAANKLGVNVVRVPAYSPYGVAEHAVGLILSLNRKIHRAYNRVREGNFALDGLLGFNINGRTVGIIGTGKIGLILG
QIMKGFGCRLLAYDVYHNPEMLALGGEYVELPELFANSDIISLHCPLMPQTHHLINAEAIEQVKPGVMLINTSRGALIHT
QAVIEGLKTGKIGSLGVDVYEQESELFFEDLSGEIIQDDIFQRLTTFPNVLITGHQAFFTEDALRNIAETTLNNIADIEQ
GRSCPNEIRYQPEVEAKVLVS

Specific function: Fermentative Lactate Dehydrogenase. [C]

COG id: COG1052

COG function: function code CHR; Lactate dehydrogenase and related dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI61743967, Length=265, Percent_Identity=32.8301886792453, Blast_Score=127, Evalue=1e-29,
Organism=Homo sapiens, GI4557497, Length=265, Percent_Identity=32.8301886792453, Blast_Score=127, Evalue=2e-29,
Organism=Homo sapiens, GI23308577, Length=280, Percent_Identity=28.5714285714286, Blast_Score=127, Evalue=2e-29,
Organism=Homo sapiens, GI145580578, Length=272, Percent_Identity=31.6176470588235, Blast_Score=122, Evalue=4e-28,
Organism=Homo sapiens, GI4557499, Length=272, Percent_Identity=31.6176470588235, Blast_Score=122, Evalue=4e-28,
Organism=Homo sapiens, GI145580575, Length=269, Percent_Identity=31.9702602230483, Blast_Score=121, Evalue=1e-27,
Organism=Homo sapiens, GI6912396, Length=292, Percent_Identity=28.4246575342466, Blast_Score=102, Evalue=6e-22,
Organism=Escherichia coli, GI1787645, Length=328, Percent_Identity=57.3170731707317, Blast_Score=402, Evalue=1e-113,
Organism=Escherichia coli, GI1789279, Length=232, Percent_Identity=32.3275862068966, Blast_Score=108, Evalue=7e-25,
Organism=Escherichia coli, GI87082289, Length=275, Percent_Identity=28.7272727272727, Blast_Score=105, Evalue=3e-24,
Organism=Escherichia coli, GI1788660, Length=261, Percent_Identity=27.5862068965517, Blast_Score=66, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI17532191, Length=265, Percent_Identity=29.811320754717, Blast_Score=122, Evalue=3e-28,
Organism=Caenorhabditis elegans, GI25147481, Length=265, Percent_Identity=29.4339622641509, Blast_Score=112, Evalue=4e-25,
Organism=Saccharomyces cerevisiae, GI6324055, Length=202, Percent_Identity=38.6138613861386, Blast_Score=130, Evalue=3e-31,
Organism=Saccharomyces cerevisiae, GI6322116, Length=238, Percent_Identity=31.9327731092437, Blast_Score=114, Evalue=2e-26,
Organism=Saccharomyces cerevisiae, GI6320925, Length=238, Percent_Identity=31.0924369747899, Blast_Score=110, Evalue=3e-25,
Organism=Saccharomyces cerevisiae, GI6324964, Length=212, Percent_Identity=27.8301886792453, Blast_Score=92, Evalue=9e-20,
Organism=Saccharomyces cerevisiae, GI6324980, Length=140, Percent_Identity=27.1428571428571, Blast_Score=68, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6321253, Length=371, Percent_Identity=24.7978436657682, Blast_Score=67, Evalue=5e-12,
Organism=Drosophila melanogaster, GI24646446, Length=282, Percent_Identity=35.1063829787234, Blast_Score=134, Evalue=7e-32,
Organism=Drosophila melanogaster, GI24646448, Length=282, Percent_Identity=35.1063829787234, Blast_Score=134, Evalue=7e-32,
Organism=Drosophila melanogaster, GI24646452, Length=282, Percent_Identity=35.1063829787234, Blast_Score=134, Evalue=7e-32,
Organism=Drosophila melanogaster, GI24646450, Length=282, Percent_Identity=35.1063829787234, Blast_Score=134, Evalue=7e-32,
Organism=Drosophila melanogaster, GI62472511, Length=282, Percent_Identity=35.1063829787234, Blast_Score=133, Evalue=2e-31,
Organism=Drosophila melanogaster, GI19921140, Length=252, Percent_Identity=32.1428571428571, Blast_Score=126, Evalue=2e-29,
Organism=Drosophila melanogaster, GI28571528, Length=230, Percent_Identity=29.5652173913043, Blast_Score=92, Evalue=4e-19,
Organism=Drosophila melanogaster, GI28574286, Length=252, Percent_Identity=26.5873015873016, Blast_Score=91, Evalue=2e-18,
Organism=Drosophila melanogaster, GI45552429, Length=269, Percent_Identity=25.6505576208178, Blast_Score=87, Evalue=1e-17,
Organism=Drosophila melanogaster, GI24585514, Length=269, Percent_Identity=25.6505576208178, Blast_Score=87, Evalue=1e-17,
Organism=Drosophila melanogaster, GI28574282, Length=269, Percent_Identity=25.6505576208178, Blast_Score=87, Evalue=1e-17,
Organism=Drosophila melanogaster, GI28574284, Length=217, Percent_Identity=27.6497695852535, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI45551003, Length=217, Percent_Identity=27.6497695852535, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24585516, Length=221, Percent_Identity=24.4343891402715, Blast_Score=70, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006139
- InterPro:   IPR006140
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00389 2-Hacid_dh; PF02826 2-Hacid_dh_C [H]

EC number: 1.1.1.28

Molecular weight: Translated: 37426; Mature: 37426

Theoretical pI: Translated: 5.79; Mature: 5.79

Prosite motif: PS00065 D_2_HYDROXYACID_DH_1 ; PS00670 D_2_HYDROXYACID_DH_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVAVFSTKAYDRQFLEAANAPKQHDLVFFEPRLNQDTAILAAGFPAVCVFVHDQVDAAT
CEEEEEECHHHHHHHHHHCCCCCCCCEEEECCCCCCCCEEEEECCCEEEEEEECCCCHHH
LEILASRGTRLIVLRCAGFNNVDLKAANKLGVNVVRVPAYSPYGVAEHAVGLILSLNRKI
HHHHHCCCCEEEEEEECCCCCCCEEECCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHH
HRAYNRVREGNFALDGLLGFNINGRTVGIIGTGKIGLILGQIMKGFGCRLLAYDVYHNPE
HHHHHHHHCCCEEEEEEEEECCCCEEEEEEECCHHHHHHHHHHHCCCCEEEEEEEECCCE
MLALGGEYVELPELFANSDIISLHCPLMPQTHHLINAEAIEQVKPGVMLINTSRGALIHT
EEEECCCEEECHHHHCCCCEEEEECCCCCCCHHHCCHHHHHHCCCCEEEEECCCCCEEEH
QAVIEGLKTGKIGSLGVDVYEQESELFFEDLSGEIIQDDIFQRLTTFPNVLITGHQAFFT
HHHHHHHCCCCCCCCCCEEEECCHHHHHHHCCCCHHHHHHHHHHHCCCCEEEECCHHHHH
EDALRNIAETTLNNIADIEQGRSCPNEIRYQPEVEAKVLVS
HHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCCCEEEEEC
>Mature Secondary Structure
MKVAVFSTKAYDRQFLEAANAPKQHDLVFFEPRLNQDTAILAAGFPAVCVFVHDQVDAAT
CEEEEEECHHHHHHHHHHCCCCCCCCEEEECCCCCCCCEEEEECCCEEEEEEECCCCHHH
LEILASRGTRLIVLRCAGFNNVDLKAANKLGVNVVRVPAYSPYGVAEHAVGLILSLNRKI
HHHHHCCCCEEEEEEECCCCCCCEEECCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHH
HRAYNRVREGNFALDGLLGFNINGRTVGIIGTGKIGLILGQIMKGFGCRLLAYDVYHNPE
HHHHHHHHCCCEEEEEEEEECCCCEEEEEEECCHHHHHHHHHHHCCCCEEEEEEEECCCE
MLALGGEYVELPELFANSDIISLHCPLMPQTHHLINAEAIEQVKPGVMLINTSRGALIHT
EEEECCCEEECHHHHCCCCEEEEECCCCCCCHHHCCHHHHHHCCCCEEEEECCCCCEEEH
QAVIEGLKTGKIGSLGVDVYEQESELFFEDLSGEIIQDDIFQRLTTFPNVLITGHQAFFT
HHHHHHHCCCCCCCCCCEEEECCHHHHHHHCCCCHHHHHHHHHHHCCCCEEEECCHHHHH
EDALRNIAETTLNNIADIEQGRSCPNEIRYQPEVEAKVLVS
HHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: (R)-lactate; NAD+

Specific reaction: (R)-lactate + NAD+ = pyruvate + NADH + H+

General reaction: Redox reaction [C]

Inhibitor: Pyruvate [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]