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The map label for this gene is glcD [H]

Identifier: 75908734

GI number: 75908734

Start: 3115941

End: 3117419

Strand: Direct

Name: glcD [H]

Synonym: Ava_2520

Alternate gene names: 75908734

Gene position: 3115941-3117419 (Clockwise)

Preceding gene: 75908733

Following gene: 75908735

Centisome position: 48.95

GC content: 42.33

Gene sequence:

>1479_bases
ATGCTTACCCAAGACCAAGATCAGAAACAACGTAACTGGAAACCCATTATCAAAGCATTCGAGGCTGTCCTGGGTACAAA
TGGCGTAGTCCAACGCCGTGAGGAACTTATCACTTATGAGTGTGATGGTTTAACTAGCTATCGCCAACGTCCGGCTGTGG
CGGTGTTACCTAGAACGACAGAACAAGTGGCGGCGGTAGTTAAAATCTGTAACCAGTATGCTGTACCCTTCATTGCGCGG
GGTTCTGGTACTGGTTTATCTGGTGGTGCTTTACCATCGGAAGATTCAGTTTTAATTGTTACTTCTTTAATGCGGCAAAT
TCTGAATGTTGATTTAGATAATCAGCGCATAGTTGTACAACCAGGAGTAATTAACAGTTGGGTGACACAAACTGTTAGTG
GTGCGGGATTTTATTATGCGCCAGACCCTTCTAGTCAAATTATCTGTTCAATTGGGGGTAACGTTGCGGAAAACTCTGGC
GGGGTACATTGTCTAAAATATGGTGTCACAACAAACCACGTTTTGGGTTTGAAAATCGTCACACCTGAAGGGGAAATAGT
TGATTTAGGTGGACAAATTCCTGAAGCCCCTGGTTATGACTTAACTGGTATATTTGTCGGTTCGGAAGGTACTTTAGGGA
TTGCTACAGAAATTACTTTGCGGATTCTCAAAAGTGCAGAATCAATCTGTGTATTATTAGCAGACTTTACCAGTGTGGAA
GCGGCTGGGGCGACTGTTTCTGATATCATCAGCGCGGGGATAATTCCTGGTGGGATGGAAATGATGGATAACGTTAGTAT
TAATGCAGTGGAAGATGTTGTCGCCACTAATTGTTATCCGCGTGATGCCACAGCGATTTTGTTAGTAGAAATTGATGGTT
TAGATGTCGAAGTTGCCGTGAATAAACAACGGGTGACTGACATTTGTCAACAAAATGGGGCGCGTAGTGTGACTTCTGCT
AGTGACCCAGAAACTAGATTGAAATTATGGAAAGGGCGTAAGGCGGCTTTTGCTGCGGCTGGTCATTTAAGCCCAGATTA
TTATGTGCAGGATGGTGTCATTCCTCGGACTCAGTTACCTTATGTATTGCAGGAAATTGAGGCATTGAGTCAAAAATTTG
GGTATCCAATTGCCAATGTTTTTCATGCTGGTGATGGTAATCTGCATCCATTGATTCTATTTGATAATTCCATTTCTGGT
GCATTAGAGAAAGTCGAAGAATTGGGAGGAGAAATTCTCAAACTTTGTGTGAGAGTCGGGGGTAGTATTTCTGGAGAACA
TGGTATTGGGGCAGATAAAAAATGTTATATGCCTGATATGTTTAGTGATGTTGATTTAGAAACAATGCAGTGGATCAGAC
AAGTATTTAATCCCCAAGGTTTGGCTAATCCTGGAAAGATATTCCCCACACCGCGCACTTGTGGCGAAGCTGCAAATGCT
ATTAAAAATCAACATTTTGAAGGAGTAGAAAGATTTTAA

Upstream 100 bases:

>100_bases
CTTACTTAGAATCAGTGCGATCGCCTCTTCACCCCCTGAAACTGCTATTCTTGCAACAATAGTAATTAAACTCCTCCACC
AGTCTGTTAAATAAAAATCT

Downstream 100 bases:

>100_bases
TACTCATCAATTAACAACAAAAATATTTATTTGTAGTAAGGGCTAAAGCCCTTATTTTTTTAAAGAACTTGAAAAAATAA
GATTTCATGTGTAAAACAAT

Product: glycolate oxidase subunit GlcD

Products: 2-oxo acid; H2O2

Alternate protein names: NA

Number of amino acids: Translated: 492; Mature: 492

Protein sequence:

>492_residues
MLTQDQDQKQRNWKPIIKAFEAVLGTNGVVQRREELITYECDGLTSYRQRPAVAVLPRTTEQVAAVVKICNQYAVPFIAR
GSGTGLSGGALPSEDSVLIVTSLMRQILNVDLDNQRIVVQPGVINSWVTQTVSGAGFYYAPDPSSQIICSIGGNVAENSG
GVHCLKYGVTTNHVLGLKIVTPEGEIVDLGGQIPEAPGYDLTGIFVGSEGTLGIATEITLRILKSAESICVLLADFTSVE
AAGATVSDIISAGIIPGGMEMMDNVSINAVEDVVATNCYPRDATAILLVEIDGLDVEVAVNKQRVTDICQQNGARSVTSA
SDPETRLKLWKGRKAAFAAAGHLSPDYYVQDGVIPRTQLPYVLQEIEALSQKFGYPIANVFHAGDGNLHPLILFDNSISG
ALEKVEELGGEILKLCVRVGGSISGEHGIGADKKCYMPDMFSDVDLETMQWIRQVFNPQGLANPGKIFPTPRTCGEAANA
IKNQHFEGVERF

Sequences:

>Translated_492_residues
MLTQDQDQKQRNWKPIIKAFEAVLGTNGVVQRREELITYECDGLTSYRQRPAVAVLPRTTEQVAAVVKICNQYAVPFIAR
GSGTGLSGGALPSEDSVLIVTSLMRQILNVDLDNQRIVVQPGVINSWVTQTVSGAGFYYAPDPSSQIICSIGGNVAENSG
GVHCLKYGVTTNHVLGLKIVTPEGEIVDLGGQIPEAPGYDLTGIFVGSEGTLGIATEITLRILKSAESICVLLADFTSVE
AAGATVSDIISAGIIPGGMEMMDNVSINAVEDVVATNCYPRDATAILLVEIDGLDVEVAVNKQRVTDICQQNGARSVTSA
SDPETRLKLWKGRKAAFAAAGHLSPDYYVQDGVIPRTQLPYVLQEIEALSQKFGYPIANVFHAGDGNLHPLILFDNSISG
ALEKVEELGGEILKLCVRVGGSISGEHGIGADKKCYMPDMFSDVDLETMQWIRQVFNPQGLANPGKIFPTPRTCGEAANA
IKNQHFEGVERF
>Mature_492_residues
MLTQDQDQKQRNWKPIIKAFEAVLGTNGVVQRREELITYECDGLTSYRQRPAVAVLPRTTEQVAAVVKICNQYAVPFIAR
GSGTGLSGGALPSEDSVLIVTSLMRQILNVDLDNQRIVVQPGVINSWVTQTVSGAGFYYAPDPSSQIICSIGGNVAENSG
GVHCLKYGVTTNHVLGLKIVTPEGEIVDLGGQIPEAPGYDLTGIFVGSEGTLGIATEITLRILKSAESICVLLADFTSVE
AAGATVSDIISAGIIPGGMEMMDNVSINAVEDVVATNCYPRDATAILLVEIDGLDVEVAVNKQRVTDICQQNGARSVTSA
SDPETRLKLWKGRKAAFAAAGHLSPDYYVQDGVIPRTQLPYVLQEIEALSQKFGYPIANVFHAGDGNLHPLILFDNSISG
ALEKVEELGGEILKLCVRVGGSISGEHGIGADKKCYMPDMFSDVDLETMQWIRQVFNPQGLANPGKIFPTPRTCGEAANA
IKNQHFEGVERF

Specific function: Unknown

COG id: COG0277

COG function: function code C; FAD/FMN-containing dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FAD-binding PCMH-type domain [H]

Homologues:

Organism=Homo sapiens, GI37595756, Length=477, Percent_Identity=30.398322851153, Blast_Score=223, Evalue=3e-58,
Organism=Homo sapiens, GI37595754, Length=501, Percent_Identity=29.3413173652695, Blast_Score=217, Evalue=2e-56,
Organism=Homo sapiens, GI119964728, Length=474, Percent_Identity=28.0590717299578, Blast_Score=152, Evalue=5e-37,
Organism=Homo sapiens, GI4501993, Length=457, Percent_Identity=22.9759299781182, Blast_Score=91, Evalue=2e-18,
Organism=Escherichia coli, GI1789351, Length=467, Percent_Identity=51.6059957173448, Blast_Score=481, Evalue=1e-137,
Organism=Escherichia coli, GI1787977, Length=500, Percent_Identity=24.4, Blast_Score=115, Evalue=8e-27,
Organism=Escherichia coli, GI48994907, Length=447, Percent_Identity=23.0425055928412, Blast_Score=94, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI71992373, Length=429, Percent_Identity=28.6713286713287, Blast_Score=194, Evalue=7e-50,
Organism=Caenorhabditis elegans, GI71992381, Length=428, Percent_Identity=27.1028037383178, Blast_Score=175, Evalue=4e-44,
Organism=Caenorhabditis elegans, GI71992392, Length=358, Percent_Identity=27.0949720670391, Blast_Score=158, Evalue=5e-39,
Organism=Caenorhabditis elegans, GI17534361, Length=466, Percent_Identity=25.5364806866953, Blast_Score=134, Evalue=1e-31,
Organism=Caenorhabditis elegans, GI17556096, Length=456, Percent_Identity=21.2719298245614, Blast_Score=79, Evalue=5e-15,
Organism=Saccharomyces cerevisiae, GI6320027, Length=437, Percent_Identity=28.3752860411899, Blast_Score=187, Evalue=4e-48,
Organism=Saccharomyces cerevisiae, GI6320764, Length=477, Percent_Identity=25.5765199161426, Blast_Score=149, Evalue=8e-37,
Organism=Saccharomyces cerevisiae, GI6320023, Length=479, Percent_Identity=25.4697286012526, Blast_Score=140, Evalue=3e-34,
Organism=Drosophila melanogaster, GI18921117, Length=490, Percent_Identity=27.7551020408163, Blast_Score=157, Evalue=2e-38,
Organism=Drosophila melanogaster, GI24639277, Length=490, Percent_Identity=27.7551020408163, Blast_Score=157, Evalue=2e-38,
Organism=Drosophila melanogaster, GI24639275, Length=490, Percent_Identity=27.7551020408163, Blast_Score=157, Evalue=2e-38,
Organism=Drosophila melanogaster, GI24653753, Length=224, Percent_Identity=26.3392857142857, Blast_Score=87, Evalue=3e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016166
- InterPro:   IPR016167
- InterPro:   IPR016164
- InterPro:   IPR016168
- InterPro:   IPR004113
- InterPro:   IPR004490
- InterPro:   IPR006094
- InterPro:   IPR016171 [H]

Pfam domain/function: PF02913 FAD-oxidase_C; PF01565 FAD_binding_4 [H]

EC number: 1.1.3.15

Molecular weight: Translated: 52765; Mature: 52765

Theoretical pI: Translated: 4.63; Mature: 4.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTQDQDQKQRNWKPIIKAFEAVLGTNGVVQRREELITYECDGLTSYRQRPAVAVLPRTT
CCCCCCHHHHCCHHHHHHHHHHHHCCCHHHHHHHHHEEEECCCCHHHHHCCCEEECCCCH
EQVAAVVKICNQYAVPFIARGSGTGLSGGALPSEDSVLIVTSLMRQILNVDLDNQRIVVQ
HHHHHHHHHHHHHCCCEEECCCCCCCCCCCCCCCCCEEHHHHHHHHHHCCCCCCCEEEEE
PGVINSWVTQTVSGAGFYYAPDPSSQIICSIGGNVAENSGGVHCLKYGVTTNHVLGLKIV
CCCHHHHHHHHHCCCCEEECCCCCCCEEEEECCCCCCCCCCEEEEEECCCCCCEEEEEEE
TPEGEIVDLGGQIPEAPGYDLTGIFVGSEGTLGIATEITLRILKSAESICVLLADFTSVE
CCCCCEEECCCCCCCCCCCCEEEEEECCCCCEEHHHHHHHHHHHCCCCEEEEEEHHHHHH
AAGATVSDIISAGIIPGGMEMMDNVSINAVEDVVATNCYPRDATAILLVEIDGLDVEVAV
HCCCHHHHHHHHCCCCCCHHHHHCCCCHHHHHHHHCCCCCCCCCEEEEEEECCCEEEEEE
NKQRVTDICQQNGARSVTSASDPETRLKLWKGRKAAFAAAGHLSPDYYVQDGVIPRTQLP
CHHHHHHHHHCCCCCCCCCCCCCHHHHHHHCCCCHHEEECCCCCCCCEEECCCCCCCCCH
YVLQEIEALSQKFGYPIANVFHAGDGNLHPLILFDNSISGALEKVEELGGEILKLCVRVG
HHHHHHHHHHHHHCCCHHHEEECCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHC
GSISGEHGIGADKKCYMPDMFSDVDLETMQWIRQVFNPQGLANPGKIFPTPRTCGEAANA
CCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHH
IKNQHFEGVERF
HHHHCCHHHHCC
>Mature Secondary Structure
MLTQDQDQKQRNWKPIIKAFEAVLGTNGVVQRREELITYECDGLTSYRQRPAVAVLPRTT
CCCCCCHHHHCCHHHHHHHHHHHHCCCHHHHHHHHHEEEECCCCHHHHHCCCEEECCCCH
EQVAAVVKICNQYAVPFIARGSGTGLSGGALPSEDSVLIVTSLMRQILNVDLDNQRIVVQ
HHHHHHHHHHHHHCCCEEECCCCCCCCCCCCCCCCCEEHHHHHHHHHHCCCCCCCEEEEE
PGVINSWVTQTVSGAGFYYAPDPSSQIICSIGGNVAENSGGVHCLKYGVTTNHVLGLKIV
CCCHHHHHHHHHCCCCEEECCCCCCCEEEEECCCCCCCCCCEEEEEECCCCCCEEEEEEE
TPEGEIVDLGGQIPEAPGYDLTGIFVGSEGTLGIATEITLRILKSAESICVLLADFTSVE
CCCCCEEECCCCCCCCCCCCEEEEEECCCCCEEHHHHHHHHHHHCCCCEEEEEEHHHHHH
AAGATVSDIISAGIIPGGMEMMDNVSINAVEDVVATNCYPRDATAILLVEIDGLDVEVAV
HCCCHHHHHHHHCCCCCCHHHHHCCCCHHHHHHHHCCCCCCCCCEEEEEEECCCEEEEEE
NKQRVTDICQQNGARSVTSASDPETRLKLWKGRKAAFAAAGHLSPDYYVQDGVIPRTQLP
CHHHHHHHHHCCCCCCCCCCCCCHHHHHHHCCCCHHEEECCCCCCCCEEECCCCCCCCCH
YVLQEIEALSQKFGYPIANVFHAGDGNLHPLILFDNSISGALEKVEELGGEILKLCVRVG
HHHHHHHHHHHHHCCCHHHEEECCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHC
GSISGEHGIGADKKCYMPDMFSDVDLETMQWIRQVFNPQGLANPGKIFPTPRTCGEAANA
CCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHH
IKNQHFEGVERF
HHHHCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: (S)-2-hydroxy acid; O2

Specific reaction: (S)-2-hydroxy acid + O2 = 2-oxo acid + H2O2

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]