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The map label for this gene is proS

Identifier: 75908528

GI number: 75908528

Start: 2872157

End: 2873971

Strand: Direct

Name: proS

Synonym: Ava_2311

Alternate gene names: 75908528

Gene position: 2872157-2873971 (Clockwise)

Preceding gene: 75908526

Following gene: 75908529

Centisome position: 45.12

GC content: 44.13

Gene sequence:

>1815_bases
ATGCGACTGTCACAAATGTTATTCGTTACACTACGGGATGATCCGGCTGATGCGGAGATTCCTAGCCATAAGTTGTTACT
ACGTGCAGGCTACATTCGTCGCATCGGTAGCGGTATTTATGCGTATCTCCCCCTGATGTGGCGAGTACTGCAAAAGGTTT
CTCAAATTGTGCGGGAAGAAATGAACGCGGCAGGCGCACAAGAATGTCTGTTACCCCAGTTACAGCCGTCGGAGTTGTGG
AAGGAGTCGGGACGCTGGGATACTTATACTAAAGCCGAGGGAATTATGTTTTCCCTAATTGACCGCCGCGAACAACAATT
AGGGTTAGGGCCGACGCACGAAGAAGTAATTACAGCGATCGCTCGTGATATGATTCGCTCTTACCGTCAGCTACCCCTAC
ATCTATATCAATTGCAAACCAAGTTCCGCGATGAAATACGTCCCCGGTTTGGTTTGATGCGCGGACGGGAATTTATTATG
AAGGATGGCTATTCTTTCCACGTCGATGAAGACAGCCTGAAAAAAACCTACCAAGATATGTACCAAGCCTACAGCAATAT
GCTACGGCGCGCGGGTTTAGCGTTTCGTCCGGTAGAAGCTGATTCTGGTGCAATTGGTGGTTCTGGTTCTACAGAATTTA
TGGTGTTGGCGGAAGCTGGGGAAGACGAAGTTCTCTACACCGATGATGGGAAATACGCCGCTAATGTGGAAAAAGCGGTT
TCTTTACCTGCGGATGCGGAACCGTCACAGTTTACAGCTTTTGAAAAGCGAGAAACACCAGGAACAGAAACTATTGAAAA
AGTTACTCAATTTCTCAAAGCTTCTCCTACCCAGATAGTGAAAAATGTTCTCTACCAAACGGTTTATGACAATGGTGTAA
CAGTTTTGGTACTGGTAATTATTCGTGGTGATCAAGAAGTTAACGAAGTTAAGTTACAGAATGAATTGACGAAATTAGCT
CCTAATTACGGTGCAAAGGCAATTATTAACCTCACCGTTCCCAGTGCAGAAAACCAACAAACATGGACAGCAAAATCTTT
ACCTTTGGGCTATATTGCACCGGATATTGCTGATGAGTATATTGCTGCAAATAAACAAATTCATCCTAAATTTGTCCGGT
TCGTTGATAAAACCGCCGTTGATTTAAAAAACTTCATTACAGGTGCAAATGAAGCTGGTTATCACGTTGTGGGTGCGAAT
TGGGGCGAACAATTTCCCCTACCAGAAATTGTTGTAGATGTGCGTAAAGCCAGGCCAGGCGATCGCGCTATTCATGACTC
AACCCAAATCTTAAAAAGTGCCAGAGGAATTGAAGTCGGTCATATCTTCCAATTAGGTACTAAATATTCCCAAGCCTTAG
GCGCAACCTATACCAATGAACAAGGTGAAGAAAAACCCCTGGTGATGGGTTGCTACGGTGTGGGTGTATCGCGGTTAGCC
CAATCAGCCGTGGAACAATCTTATGACAAAGATGGCATTATCTGGCCCGTGGCGATCGCTCCTTATCATGCAATTGTGAC
AATTCCTAACATTAACGATGCTCAACAGGTAGAAATTGCGGAAAGGCTTTACACTGAACTGAATCAATCAGGGGTAGAAA
CTTTATTAGATGACCGTAATGAAAGAGCCGGAGTCAAATTCAAAGACGCTGATCTAATCGGTATTCCTTACAGAATTGTT
ACCGGACGAGCAATTACTAACGGTAAAGTTGAAATTGTAGAAAGGGCAACTCGCCAATCTCAAGAAATCCCCATTGATGA
AGTTATTACCACACTCCAGCAGTGGATTAAAGCCGCAATAGAGCAGAAAAATTAA

Upstream 100 bases:

>100_bases
AATAACCTATATTTTGTAATTCATAGTTCATGTTCGATATTTCGTAATTGAGTTGATACAGAAGTTAGTGATTTTGCAAT
TAGGAGTCAAGGAATAAACG

Downstream 100 bases:

>100_bases
ATTATGAGGAAATGAGACTATTTATTACTCTCATTTCCTCTGTATCTCTCATTCAACTGTTGAAAACTTGAAGAAATCAT
AACATTTACAGATTTACATA

Product: prolyl-tRNA synthetase

Products: NA

Alternate protein names: Proline--tRNA ligase; ProRS

Number of amino acids: Translated: 604; Mature: 604

Protein sequence:

>604_residues
MRLSQMLFVTLRDDPADAEIPSHKLLLRAGYIRRIGSGIYAYLPLMWRVLQKVSQIVREEMNAAGAQECLLPQLQPSELW
KESGRWDTYTKAEGIMFSLIDRREQQLGLGPTHEEVITAIARDMIRSYRQLPLHLYQLQTKFRDEIRPRFGLMRGREFIM
KDGYSFHVDEDSLKKTYQDMYQAYSNMLRRAGLAFRPVEADSGAIGGSGSTEFMVLAEAGEDEVLYTDDGKYAANVEKAV
SLPADAEPSQFTAFEKRETPGTETIEKVTQFLKASPTQIVKNVLYQTVYDNGVTVLVLVIIRGDQEVNEVKLQNELTKLA
PNYGAKAIINLTVPSAENQQTWTAKSLPLGYIAPDIADEYIAANKQIHPKFVRFVDKTAVDLKNFITGANEAGYHVVGAN
WGEQFPLPEIVVDVRKARPGDRAIHDSTQILKSARGIEVGHIFQLGTKYSQALGATYTNEQGEEKPLVMGCYGVGVSRLA
QSAVEQSYDKDGIIWPVAIAPYHAIVTIPNINDAQQVEIAERLYTELNQSGVETLLDDRNERAGVKFKDADLIGIPYRIV
TGRAITNGKVEIVERATRQSQEIPIDEVITTLQQWIKAAIEQKN

Sequences:

>Translated_604_residues
MRLSQMLFVTLRDDPADAEIPSHKLLLRAGYIRRIGSGIYAYLPLMWRVLQKVSQIVREEMNAAGAQECLLPQLQPSELW
KESGRWDTYTKAEGIMFSLIDRREQQLGLGPTHEEVITAIARDMIRSYRQLPLHLYQLQTKFRDEIRPRFGLMRGREFIM
KDGYSFHVDEDSLKKTYQDMYQAYSNMLRRAGLAFRPVEADSGAIGGSGSTEFMVLAEAGEDEVLYTDDGKYAANVEKAV
SLPADAEPSQFTAFEKRETPGTETIEKVTQFLKASPTQIVKNVLYQTVYDNGVTVLVLVIIRGDQEVNEVKLQNELTKLA
PNYGAKAIINLTVPSAENQQTWTAKSLPLGYIAPDIADEYIAANKQIHPKFVRFVDKTAVDLKNFITGANEAGYHVVGAN
WGEQFPLPEIVVDVRKARPGDRAIHDSTQILKSARGIEVGHIFQLGTKYSQALGATYTNEQGEEKPLVMGCYGVGVSRLA
QSAVEQSYDKDGIIWPVAIAPYHAIVTIPNINDAQQVEIAERLYTELNQSGVETLLDDRNERAGVKFKDADLIGIPYRIV
TGRAITNGKVEIVERATRQSQEIPIDEVITTLQQWIKAAIEQKN
>Mature_604_residues
MRLSQMLFVTLRDDPADAEIPSHKLLLRAGYIRRIGSGIYAYLPLMWRVLQKVSQIVREEMNAAGAQECLLPQLQPSELW
KESGRWDTYTKAEGIMFSLIDRREQQLGLGPTHEEVITAIARDMIRSYRQLPLHLYQLQTKFRDEIRPRFGLMRGREFIM
KDGYSFHVDEDSLKKTYQDMYQAYSNMLRRAGLAFRPVEADSGAIGGSGSTEFMVLAEAGEDEVLYTDDGKYAANVEKAV
SLPADAEPSQFTAFEKRETPGTETIEKVTQFLKASPTQIVKNVLYQTVYDNGVTVLVLVIIRGDQEVNEVKLQNELTKLA
PNYGAKAIINLTVPSAENQQTWTAKSLPLGYIAPDIADEYIAANKQIHPKFVRFVDKTAVDLKNFITGANEAGYHVVGAN
WGEQFPLPEIVVDVRKARPGDRAIHDSTQILKSARGIEVGHIFQLGTKYSQALGATYTNEQGEEKPLVMGCYGVGVSRLA
QSAVEQSYDKDGIIWPVAIAPYHAIVTIPNINDAQQVEIAERLYTELNQSGVETLLDDRNERAGVKFKDADLIGIPYRIV
TGRAITNGKVEIVERATRQSQEIPIDEVITTLQQWIKAAIEQKN

Specific function: Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction:proline is first activated by ATP to form Pro- AMP and then transferred to the acceptor end of tRNA(Pro). As ProRS can inadvertently accommodate and process non-cognate amino acids su

COG id: COG0442

COG function: function code J; Prolyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 1 subfamily

Homologues:

Organism=Homo sapiens, GI34303926, Length=230, Percent_Identity=41.7391304347826, Blast_Score=187, Evalue=2e-47,
Organism=Escherichia coli, GI1786392, Length=602, Percent_Identity=44.5182724252492, Blast_Score=486, Evalue=1e-138,
Organism=Caenorhabditis elegans, GI115532348, Length=214, Percent_Identity=36.4485981308411, Blast_Score=135, Evalue=4e-32,
Organism=Saccharomyces cerevisiae, GI6320931, Length=242, Percent_Identity=34.7107438016529, Blast_Score=155, Evalue=1e-38,
Organism=Drosophila melanogaster, GI24656200, Length=226, Percent_Identity=39.3805309734513, Blast_Score=163, Evalue=3e-40,

Paralogues:

None

Copy number: 800 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): SYP_ANAVT (Q3MAQ7)

Other databases:

- EMBL:   CP000117
- RefSeq:   YP_322824.1
- ProteinModelPortal:   Q3MAQ7
- SMR:   Q3MAQ7
- STRING:   Q3MAQ7
- GeneID:   3678919
- GenomeReviews:   CP000117_GR
- KEGG:   ava:Ava_2311
- NMPDR:   fig|240292.3.peg.191
- eggNOG:   COG0442
- HOGENOM:   HBG403504
- OMA:   DFVLGPT
- PhylomeDB:   Q3MAQ7
- ProtClustDB:   PRK09194
- BioCyc:   AVAR240292:AVA_2311-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01569
- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR002316
- InterPro:   IPR004500
- InterPro:   IPR007214
- Gene3D:   G3DSA:3.40.50.800
- PRINTS:   PR01046
- TIGRFAMs:   TIGR00409

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b; PF04073 YbaK; SSF52954 Anticodon_bd; SSF55826 YbaK/aa-tRNA-synth-assoc-reg

EC number: =6.1.1.15

Molecular weight: Translated: 67689; Mature: 67689

Theoretical pI: Translated: 5.36; Mature: 5.36

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLSQMLFVTLRDDPADAEIPSHKLLLRAGYIRRIGSGIYAYLPLMWRVLQKVSQIVREE
CCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHH
MNAAGAQECLLPQLQPSELWKESGRWDTYTKAEGIMFSLIDRREQQLGLGPTHEEVITAI
HHCCCHHHHCCCCCCHHHHHHHCCCCCCCHHHCCHHHHHHHHHHHHCCCCCCHHHHHHHH
ARDMIRSYRQLPLHLYQLQTKFRDEIRPRFGLMRGREFIMKDGYSFHVDEDSLKKTYQDM
HHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCEEECCHHHHHHHHHHH
YQAYSNMLRRAGLAFRPVEADSGAIGGSGSTEFMVLAEAGEDEVLYTDDGKYAANVEKAV
HHHHHHHHHHCCCEEECCCCCCCCCCCCCCCEEEEEEECCCCCEEEECCCCCHHCHHHHH
SLPADAEPSQFTAFEKRETPGTETIEKVTQFLKASPTQIVKNVLYQTVYDNGVTVLVLVI
CCCCCCCCCCCCHHHCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCEEEEEEE
IRGDQEVNEVKLQNELTKLAPNYGAKAIINLTVPSAENQQTWTAKSLPLGYIAPDIADEY
ECCCCHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCEECCCCCCCCCCHHHHHHH
IAANKQIHPKFVRFVDKTAVDLKNFITGANEAGYHVVGANWGEQFPLPEIVVDVRKARPG
HHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCHHHHHHHHHCCCC
DRAIHDSTQILKSARGIEVGHIFQLGTKYSQALGATYTNEQGEEKPLVMGCYGVGVSRLA
CCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEHHHHHHHHH
QSAVEQSYDKDGIIWPVAIAPYHAIVTIPNINDAQQVEIAERLYTELNQSGVETLLDDRN
HHHHHHCCCCCCCEEEEEECCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCC
ERAGVKFKDADLIGIPYRIVTGRAITNGKVEIVERATRQSQEIPIDEVITTLQQWIKAAI
CCCCCEEECCCEECCCEEEEECCEECCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
EQKN
CCCC
>Mature Secondary Structure
MRLSQMLFVTLRDDPADAEIPSHKLLLRAGYIRRIGSGIYAYLPLMWRVLQKVSQIVREE
CCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHH
MNAAGAQECLLPQLQPSELWKESGRWDTYTKAEGIMFSLIDRREQQLGLGPTHEEVITAI
HHCCCHHHHCCCCCCHHHHHHHCCCCCCCHHHCCHHHHHHHHHHHHCCCCCCHHHHHHHH
ARDMIRSYRQLPLHLYQLQTKFRDEIRPRFGLMRGREFIMKDGYSFHVDEDSLKKTYQDM
HHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCEEECCHHHHHHHHHHH
YQAYSNMLRRAGLAFRPVEADSGAIGGSGSTEFMVLAEAGEDEVLYTDDGKYAANVEKAV
HHHHHHHHHHCCCEEECCCCCCCCCCCCCCCEEEEEEECCCCCEEEECCCCCHHCHHHHH
SLPADAEPSQFTAFEKRETPGTETIEKVTQFLKASPTQIVKNVLYQTVYDNGVTVLVLVI
CCCCCCCCCCCCHHHCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCEEEEEEE
IRGDQEVNEVKLQNELTKLAPNYGAKAIINLTVPSAENQQTWTAKSLPLGYIAPDIADEY
ECCCCHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCEECCCCCCCCCCHHHHHHH
IAANKQIHPKFVRFVDKTAVDLKNFITGANEAGYHVVGANWGEQFPLPEIVVDVRKARPG
HHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCHHHHHHHHHCCCC
DRAIHDSTQILKSARGIEVGHIFQLGTKYSQALGATYTNEQGEEKPLVMGCYGVGVSRLA
CCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEHHHHHHHHH
QSAVEQSYDKDGIIWPVAIAPYHAIVTIPNINDAQQVEIAERLYTELNQSGVETLLDDRN
HHHHHHCCCCCCCEEEEEECCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCC
ERAGVKFKDADLIGIPYRIVTGRAITNGKVEIVERATRQSQEIPIDEVITTLQQWIKAAI
CCCCCEEECCCEECCCEEEEECCEECCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
EQKN
CCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA