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The map label for this gene is yfiJ [H]

Identifier: 75908248

GI number: 75908248

Start: 2509590

End: 2510795

Strand: Direct

Name: yfiJ [H]

Synonym: Ava_2027

Alternate gene names: 75908248

Gene position: 2509590-2510795 (Clockwise)

Preceding gene: 75908246

Following gene: 75908249

Centisome position: 39.42

GC content: 35.32

Gene sequence:

>1206_bases
ATGGGTCAATATATTAAAGTTCATAACCACCCTTTCCGCTTTTTACTATATCTAGAATGGATATTGTTGGCAATATCTAT
TATCACATCGATATTGCCCTATCCCTCACCTAGATTTTCCAGTAGGTTTCCAGAAATTACGATTATTAGTCTCACAATTT
TTGGATTAATGGGACTAAGATTACCTACTAGTAATAACCTAAATAAAATAGTTTATACTGCCTGTGAAATAATTTTAATT
TTCATTACTGGACTATTTGAAGGGAGAGCAGCTCGAATGTTTCCCTTTCTCTACATCATTTTAGTGACTCGTAGTTGTTT
AATCTTTAAGTTACCTGGACGGTTATCTGTCACTTTGATTTCGTTTAGTTTGTTTTTATTCACCTTAATAAATCGATTAC
CTCGTCCTCCACTTCCACTACAAGCTCAAGAGCGTTTTCGCTTTTTCACGCTGAGTTTGACACTTGTATTTGGCTTAAGC
TTAATTTTCGTCTTGCTGTTAATGAATAGCTTATTATCAGAACGTCAGAGTCGAGAAGAACTAGCCGTTGCGAATGAAAA
ACTACGTCAATATGCTCTACGTATTGAAAACCAAGCAACTCTAGAAGAACGCAATCGCATTGCGCGGGAAATTCACGACT
CTTTAGGTCATTCGCTCACAGCATTGAATTTACAATTAGAAACTGCGCTAAAACTTGCACAGTCTAATCCCAATAAAGCA
CAAACTTTTTTAGTTAGGGCAAAAGAGCTTGGTTCTAAAGCTTTACAAGATGTGAGAAATTCTGTTTCTACTATGCGATC
TCATCCATTGCAGGACAAAACTTTAGAGCAAGCAATAGATATCATAGCAGAAGACTTTCAACAGAATACCGGGATTATAT
TAACTAGTAGTTTTAATCTTGATTATAAATTATCAACAGAAGTTAGTACAGCCATATATAGGATCATTCAAGAATCTTTA
ACTAATATTTCTAAGCACGCCAGAGCCACAGAAGTTATTTTAGAAATTTCTCTCAATCGAGGTAGCTTATCTTTGATAAT
TCGGGATAATGGTAGAGGGTTTGATATACAACAAAATACTACAGGTTTTGGATTACAAAGTATGCGCGATCGCACTTTGG
CTTTAGGAGGAAAATTTGCCATCAATAGCGATGTTGGTTATGGTTGTCAAATTACAGTTGATATTCCTGCAAGCAGATTA
ATATGA

Upstream 100 bases:

>100_bases
AGAATTGAAATAAGTTAAAAGTTTATATGGTTTGTGATGTTCTCACTATTGTCAATCCTGACTTATACATTCTTTGTCAC
TATATTTTGCAGTATAAACT

Downstream 100 bases:

>100_bases
TTAAAGTTTTATTAGTAGATGATCAAAATCTGATCCGTCAAGGATTAAGAGCATTATTAGAGCTAGAATCTGATATAGAA
ATTGTGGGAGAGGCAGAAAA

Product: periplasmic sensor signal transduction histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 401; Mature: 400

Protein sequence:

>401_residues
MGQYIKVHNHPFRFLLYLEWILLAISIITSILPYPSPRFSSRFPEITIISLTIFGLMGLRLPTSNNLNKIVYTACEIILI
FITGLFEGRAARMFPFLYIILVTRSCLIFKLPGRLSVTLISFSLFLFTLINRLPRPPLPLQAQERFRFFTLSLTLVFGLS
LIFVLLLMNSLLSERQSREELAVANEKLRQYALRIENQATLEERNRIAREIHDSLGHSLTALNLQLETALKLAQSNPNKA
QTFLVRAKELGSKALQDVRNSVSTMRSHPLQDKTLEQAIDIIAEDFQQNTGIILTSSFNLDYKLSTEVSTAIYRIIQESL
TNISKHARATEVILEISLNRGSLSLIIRDNGRGFDIQQNTTGFGLQSMRDRTLALGGKFAINSDVGYGCQITVDIPASRL
I

Sequences:

>Translated_401_residues
MGQYIKVHNHPFRFLLYLEWILLAISIITSILPYPSPRFSSRFPEITIISLTIFGLMGLRLPTSNNLNKIVYTACEIILI
FITGLFEGRAARMFPFLYIILVTRSCLIFKLPGRLSVTLISFSLFLFTLINRLPRPPLPLQAQERFRFFTLSLTLVFGLS
LIFVLLLMNSLLSERQSREELAVANEKLRQYALRIENQATLEERNRIAREIHDSLGHSLTALNLQLETALKLAQSNPNKA
QTFLVRAKELGSKALQDVRNSVSTMRSHPLQDKTLEQAIDIIAEDFQQNTGIILTSSFNLDYKLSTEVSTAIYRIIQESL
TNISKHARATEVILEISLNRGSLSLIIRDNGRGFDIQQNTTGFGLQSMRDRTLALGGKFAINSDVGYGCQITVDIPASRL
I
>Mature_400_residues
GQYIKVHNHPFRFLLYLEWILLAISIITSILPYPSPRFSSRFPEITIISLTIFGLMGLRLPTSNNLNKIVYTACEIILIF
ITGLFEGRAARMFPFLYIILVTRSCLIFKLPGRLSVTLISFSLFLFTLINRLPRPPLPLQAQERFRFFTLSLTLVFGLSL
IFVLLLMNSLLSERQSREELAVANEKLRQYALRIENQATLEERNRIAREIHDSLGHSLTALNLQLETALKLAQSNPNKAQ
TFLVRAKELGSKALQDVRNSVSTMRSHPLQDKTLEQAIDIIAEDFQQNTGIILTSSFNLDYKLSTEVSTAIYRIIQESLT
NISKHARATEVILEISLNRGSLSLIIRDNGRGFDIQQNTTGFGLQSMRDRTLALGGKFAINSDVGYGCQITVDIPASRLI

Specific function: Member of the two-component regulatory system yfiJ/yfiK. Probably activates yfiK by phosphorylation [H]

COG id: COG4585

COG function: function code T; Signal transduction histidine kinase

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI87082311, Length=224, Percent_Identity=32.1428571428571, Blast_Score=93, Evalue=3e-20,
Organism=Escherichia coli, GI1788812, Length=201, Percent_Identity=31.8407960199005, Blast_Score=77, Evalue=3e-15,
Organism=Escherichia coli, GI1787474, Length=201, Percent_Identity=26.865671641791, Blast_Score=74, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR011712 [H]

Pfam domain/function: PF02518 HATPase_c; PF07730 HisKA_3 [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 45337; Mature: 45206

Theoretical pI: Translated: 10.05; Mature: 10.05

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGQYIKVHNHPFRFLLYLEWILLAISIITSILPYPSPRFSSRFPEITIISLTIFGLMGLR
CCCEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEHHHHHHHHHHCC
LPTSNNLNKIVYTACEIILIFITGLFEGRAARMFPFLYIILVTRSCLIFKLPGRLSVTLI
CCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCEEEECCCCHHHHHH
SFSLFLFTLINRLPRPPLPLQAQERFRFFTLSLTLVFGLSLIFVLLLMNSLLSERQSREE
HHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LAVANEKLRQYALRIENQATLEERNRIAREIHDSLGHSLTALNLQLETALKLAQSNPNKA
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCEEEEEEEHHHHHHHHHCCCCHH
QTFLVRAKELGSKALQDVRNSVSTMRSHPLQDKTLEQAIDIIAEDFQQNTGIILTSSFNL
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEECCC
DYKLSTEVSTAIYRIIQESLTNISKHARATEVILEISLNRGSLSLIIRDNGRGFDIQQNT
CEEECHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEEECCCCEEEEEEECCCCCCCCCCC
TGFGLQSMRDRTLALGGKFAINSDVGYGCQITVDIPASRLI
CCCCHHHHCCHHHEECCCEEECCCCCCCEEEEEECCHHHCC
>Mature Secondary Structure 
GQYIKVHNHPFRFLLYLEWILLAISIITSILPYPSPRFSSRFPEITIISLTIFGLMGLR
CCEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEHHHHHHHHHHCC
LPTSNNLNKIVYTACEIILIFITGLFEGRAARMFPFLYIILVTRSCLIFKLPGRLSVTLI
CCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCEEEECCCCHHHHHH
SFSLFLFTLINRLPRPPLPLQAQERFRFFTLSLTLVFGLSLIFVLLLMNSLLSERQSREE
HHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LAVANEKLRQYALRIENQATLEERNRIAREIHDSLGHSLTALNLQLETALKLAQSNPNKA
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCEEEEEEEHHHHHHHHHCCCCHH
QTFLVRAKELGSKALQDVRNSVSTMRSHPLQDKTLEQAIDIIAEDFQQNTGIILTSSFNL
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEECCC
DYKLSTEVSTAIYRIIQESLTNISKHARATEVILEISLNRGSLSLIIRDNGRGFDIQQNT
CEEECHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEEECCCCEEEEEEECCCCCCCCCCC
TGFGLQSMRDRTLALGGKFAINSDVGYGCQITVDIPASRLI
CCCCHHHHCCHHHEECCCEEECCCCCCCEEEEEECCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8973323; 9384377 [H]