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The map label for this gene is relA [H]

Identifier: 75908214

GI number: 75908214

Start: 2474571

End: 2475152

Strand: Direct

Name: relA [H]

Synonym: Ava_1993

Alternate gene names: 75908214

Gene position: 2474571-2475152 (Clockwise)

Preceding gene: 75908212

Following gene: 75908216

Centisome position: 38.87

GC content: 45.36

Gene sequence:

>582_bases
ATGATTTCAGAACGCTTTACCCAAGCCTTGACCTACGCTACTCAACTCCACACTCACCAAGTCCGTAAAGGTTCGGGTAT
CCCTTACGTAGCTCATTTGTTAGGAGTTGCTAGTATTGCCTTGGAATATGGAGCCAATGAAGATGAAGCCATAGCAGCAC
TTTTACATGATGCGGTGGAAGACCAAGGTGGTGCAGAGACGCGAGAAGAAATTCGTCGCCGCTTTGGTGAAACGGTCACG
GCAATTGTTGATGGTTGTACAGACGCTGATACAATGCCAAAGCCCCCTTGGAAACAACGCAAGGAGGCTTATATTGCTCA
TATTTCTACCGCTTCTCCATCAGTGCTACTTGTATCAGCTGCTGATAAACTCCACAATGCTCGGTCAATTCTCCAAGATT
ATCGCCTTGTGGGTGAATCTCTTTGGGAACGCTTCCAAGGTGGTAAAACTGGTACTCTTTGGTATTACCGATCACTTGTG
GATGCTTATCAAAAGACTGAAACTAGGGCAATTTTTGCAGAACTGGCACGGGTAGTTACCCAAATTGAGGATTTAGCATC
TCAGAAAAAATCTGAAGCATGA

Upstream 100 bases:

>100_bases
TCATACTTAACCAAAACTAAATAACATCAGGGTCAACACTTAACACTTTTAACTTTTGTACCAATATTTTTCTAGTTCTA
AACACCACCCTCTCCTTTTT

Downstream 100 bases:

>100_bases
AGGCTGAAAAATTCTTTGCCTCATACTTCACATCTTTGTTTATGCTTCGACTGTAACAGGAAAAACAGTCTTGTTTGGGC
TAGCGGAACATACTACTAAT

Product: metal dependent phosphohydrolase

Products: NA

Alternate protein names: (p)ppGpp synthase; ATP:GTP 3'-pyrophosphotransferase; ppGpp synthase I [H]

Number of amino acids: Translated: 193; Mature: 193

Protein sequence:

>193_residues
MISERFTQALTYATQLHTHQVRKGSGIPYVAHLLGVASIALEYGANEDEAIAALLHDAVEDQGGAETREEIRRRFGETVT
AIVDGCTDADTMPKPPWKQRKEAYIAHISTASPSVLLVSAADKLHNARSILQDYRLVGESLWERFQGGKTGTLWYYRSLV
DAYQKTETRAIFAELARVVTQIEDLASQKKSEA

Sequences:

>Translated_193_residues
MISERFTQALTYATQLHTHQVRKGSGIPYVAHLLGVASIALEYGANEDEAIAALLHDAVEDQGGAETREEIRRRFGETVT
AIVDGCTDADTMPKPPWKQRKEAYIAHISTASPSVLLVSAADKLHNARSILQDYRLVGESLWERFQGGKTGTLWYYRSLV
DAYQKTETRAIFAELARVVTQIEDLASQKKSEA
>Mature_193_residues
MISERFTQALTYATQLHTHQVRKGSGIPYVAHLLGVASIALEYGANEDEAIAALLHDAVEDQGGAETREEIRRRFGETVT
AIVDGCTDADTMPKPPWKQRKEAYIAHISTASPSVLLVSAADKLHNARSILQDYRLVGESLWERFQGGKTGTLWYYRSLV
DAYQKTETRAIFAELARVVTQIEDLASQKKSEA

Specific function: In eubacteria ppGpp (guanosine 3'-diphosphate 5-' diphosphate) is a mediator of the stringent response that coordinates a variety of cellular activities in response to changes in nutritional abundance. This enzyme catalyzes the formation of pppGpp which i

COG id: COG0317

COG function: function code TK; Guanosine polyphosphate pyrophosphohydrolases/synthetases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HD domain [H]

Homologues:

Organism=Homo sapiens, GI38348360, Length=129, Percent_Identity=36.4341085271318, Blast_Score=68, Evalue=6e-12,
Organism=Escherichia coli, GI1790082, Length=133, Percent_Identity=30.8270676691729, Blast_Score=62, Evalue=4e-11,
Organism=Caenorhabditis elegans, GI25143118, Length=125, Percent_Identity=36.8, Blast_Score=71, Evalue=3e-13,
Organism=Drosophila melanogaster, GI24650996, Length=131, Percent_Identity=34.3511450381679, Blast_Score=66, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002912
- InterPro:   IPR012675
- InterPro:   IPR003607
- InterPro:   IPR007685
- InterPro:   IPR004811
- InterPro:   IPR004095
- InterPro:   IPR012676 [H]

Pfam domain/function: PF01842 ACT; PF04607 RelA_SpoT; PF02824 TGS [H]

EC number: =2.7.6.5 [H]

Molecular weight: Translated: 21436; Mature: 21436

Theoretical pI: Translated: 6.25; Mature: 6.25

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MISERFTQALTYATQLHTHQVRKGSGIPYVAHLLGVASIALEYGANEDEAIAALLHDAVE
CCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
DQGGAETREEIRRRFGETVTAIVDGCTDADTMPKPPWKQRKEAYIAHISTASPSVLLVSA
CCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCEEEEHH
ADKLHNARSILQDYRLVGESLWERFQGGKTGTLWYYRSLVDAYQKTETRAIFAELARVVT
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QIEDLASQKKSEA
HHHHHHHHHCCCC
>Mature Secondary Structure
MISERFTQALTYATQLHTHQVRKGSGIPYVAHLLGVASIALEYGANEDEAIAALLHDAVE
CCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
DQGGAETREEIRRRFGETVTAIVDGCTDADTMPKPPWKQRKEAYIAHISTASPSVLLVSA
CCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCEEEEHH
ADKLHNARSILQDYRLVGESLWERFQGGKTGTLWYYRSLVDAYQKTETRAIFAELARVVT
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QIEDLASQKKSEA
HHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9695918; 12948626 [H]