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The map label for this gene is folE [H]

Identifier: 75908170

GI number: 75908170

Start: 2423498

End: 2424148

Strand: Direct

Name: folE [H]

Synonym: Ava_1949

Alternate gene names: 75908170

Gene position: 2423498-2424148 (Clockwise)

Preceding gene: 75908169

Following gene: 75908172

Centisome position: 38.07

GC content: 41.32

Gene sequence:

>651_bases
ATGACTTTATCGATTCGTCCCGATACAGTTTCTGCTGCTCAGATGGTATCTTCTTTATCTACTCAGCAAACACCAACTGT
CACAGAAGCAGAAATGGTACAGGCTGTGCGTACTTTACTAATTGGATTAGGAGAAAATCCTGACCGTGAAGGGTTGCTAG
ATACTCCTAAGCGAGTGGTAAAAGCTTTGCAGTTTTTGACTAAGGGATACAATGAATCCTTAGATGAACTGTTAAATGGT
GCAGTATTTACAGAAGATGCCAATGAAATGGTATTGATTCGGGATATCGACATTTTCAGTTCTTGTGAACATCATATTTT
GCCAATTATTGGTCGCGCTCATGTTGCCTATATTCCCAATGGTAAGGTGATAGGATTATCTAAAATTGCGCGAGTTTGTG
AGATGTACGCTCGACGTTTACAAGTACAGGAACGTCTGACGCTACAAATTGCTGATGCACTGCAAGGTTTACTCAAACCT
CAAGGGGTAGCAGTGGTAGTAGAAGCAACTCATATGTGCATGGTGATGCGTGGTGTACAAAAGCCTGGTTCTTGGACTGT
AACTAGTGCAATGCGTGGTGTATTTGCGGAAGATGCTCGCACTCGTGAAGAGTTTATGAATTTAGTACGACATAATGCTA
ATTTTCACTAA

Upstream 100 bases:

>100_bases
GATTAAGAAGTGTTAAGTAATCAAACGACTCGGTTAATAGATTTTTGTCAATCTCTAACTATTAACTAACTCACAATCTC
TACCAATTAAACGAAATAAT

Downstream 100 bases:

>100_bases
TACCGTTTCTTAATGAAGATGTGCTTTATTCTGGACTGTAGAGACCTTTACCTGGTGCAACTTCATAGAAAATGGGTATC
AATAACCAAGATTTAGAGGA

Product: GTP cyclohydrolase I

Products: NA

Alternate protein names: GTP cyclohydrolase I 2; GTP-CH-I 2 [H]

Number of amino acids: Translated: 216; Mature: 215

Protein sequence:

>216_residues
MTLSIRPDTVSAAQMVSSLSTQQTPTVTEAEMVQAVRTLLIGLGENPDREGLLDTPKRVVKALQFLTKGYNESLDELLNG
AVFTEDANEMVLIRDIDIFSSCEHHILPIIGRAHVAYIPNGKVIGLSKIARVCEMYARRLQVQERLTLQIADALQGLLKP
QGVAVVVEATHMCMVMRGVQKPGSWTVTSAMRGVFAEDARTREEFMNLVRHNANFH

Sequences:

>Translated_216_residues
MTLSIRPDTVSAAQMVSSLSTQQTPTVTEAEMVQAVRTLLIGLGENPDREGLLDTPKRVVKALQFLTKGYNESLDELLNG
AVFTEDANEMVLIRDIDIFSSCEHHILPIIGRAHVAYIPNGKVIGLSKIARVCEMYARRLQVQERLTLQIADALQGLLKP
QGVAVVVEATHMCMVMRGVQKPGSWTVTSAMRGVFAEDARTREEFMNLVRHNANFH
>Mature_215_residues
TLSIRPDTVSAAQMVSSLSTQQTPTVTEAEMVQAVRTLLIGLGENPDREGLLDTPKRVVKALQFLTKGYNESLDELLNGA
VFTEDANEMVLIRDIDIFSSCEHHILPIIGRAHVAYIPNGKVIGLSKIARVCEMYARRLQVQERLTLQIADALQGLLKPQ
GVAVVVEATHMCMVMRGVQKPGSWTVTSAMRGVFAEDARTREEFMNLVRHNANFH

Specific function: Tetrahydrofolate biosynthesis; first step. [C]

COG id: COG0302

COG function: function code H; GTP cyclohydrolase I

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP cyclohydrolase I family [H]

Homologues:

Organism=Homo sapiens, GI66932968, Length=179, Percent_Identity=60.3351955307263, Blast_Score=239, Evalue=1e-63,
Organism=Homo sapiens, GI4503949, Length=179, Percent_Identity=60.3351955307263, Blast_Score=239, Evalue=1e-63,
Organism=Homo sapiens, GI66932970, Length=148, Percent_Identity=54.0540540540541, Blast_Score=181, Evalue=3e-46,
Organism=Homo sapiens, GI66932972, Length=139, Percent_Identity=56.8345323741007, Blast_Score=181, Evalue=6e-46,
Organism=Escherichia coli, GI1788476, Length=208, Percent_Identity=36.0576923076923, Blast_Score=124, Evalue=3e-30,
Organism=Caenorhabditis elegans, GI17560486, Length=178, Percent_Identity=58.4269662921348, Blast_Score=233, Evalue=5e-62,
Organism=Saccharomyces cerevisiae, GI6321706, Length=176, Percent_Identity=61.3636363636364, Blast_Score=224, Evalue=6e-60,
Organism=Drosophila melanogaster, GI24656782, Length=194, Percent_Identity=57.2164948453608, Blast_Score=224, Evalue=3e-59,
Organism=Drosophila melanogaster, GI24656796, Length=194, Percent_Identity=57.2164948453608, Blast_Score=224, Evalue=4e-59,
Organism=Drosophila melanogaster, GI24656791, Length=194, Percent_Identity=57.2164948453608, Blast_Score=224, Evalue=5e-59,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001474
- InterPro:   IPR020602
- InterPro:   IPR018234 [H]

Pfam domain/function: PF01227 GTP_cyclohydroI [H]

EC number: =3.5.4.16 [H]

Molecular weight: Translated: 23928; Mature: 23797

Theoretical pI: Translated: 6.79; Mature: 6.79

Prosite motif: PS00859 GTP_CYCLOHYDROL_1_1 ; PS00860 GTP_CYCLOHYDROL_1_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLSIRPDTVSAAQMVSSLSTQQTPTVTEAEMVQAVRTLLIGLGENPDREGLLDTPKRVV
CEEECCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHH
KALQFLTKGYNESLDELLNGAVFTEDANEMVLIRDIDIFSSCEHHILPIIGRAHVAYIPN
HHHHHHHCCCCHHHHHHHCCCEEECCCCCEEEEEECHHHHCCCHHHHHHHCCCEEEECCC
GKVIGLSKIARVCEMYARRLQVQERLTLQIADALQGLLKPQGVAVVVEATHMCMVMRGVQ
CCEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHCC
KPGSWTVTSAMRGVFAEDARTREEFMNLVRHNANFH
CCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
TLSIRPDTVSAAQMVSSLSTQQTPTVTEAEMVQAVRTLLIGLGENPDREGLLDTPKRVV
EEECCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHH
KALQFLTKGYNESLDELLNGAVFTEDANEMVLIRDIDIFSSCEHHILPIIGRAHVAYIPN
HHHHHHHCCCCHHHHHHHCCCEEECCCCCEEEEEECHHHHCCCHHHHHHHCCCEEEECCC
GKVIGLSKIARVCEMYARRLQVQERLTLQIADALQGLLKPQGVAVVVEATHMCMVMRGVQ
CCEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHCC
KPGSWTVTSAMRGVFAEDARTREEFMNLVRHNANFH
CCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11759840 [H]