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The map label for this gene is phoB [C]

Identifier: 75908138

GI number: 75908138

Start: 2380889

End: 2381605

Strand: Direct

Name: phoB [C]

Synonym: Ava_1917

Alternate gene names: 75908138

Gene position: 2380889-2381605 (Clockwise)

Preceding gene: 75908135

Following gene: 75908140

Centisome position: 37.4

GC content: 40.73

Gene sequence:

>717_bases
ATGGTGATGCTTTCCGCTACAAACCCCAAAATTCTTGTTGTAGATGACGATTTTGGAGTCCGTAACCTCATATATCGCTT
TTTAAGTCGCAAATATCAAATAGAGTCTGCGGCTGACGGTAAAACAGCTTTGTCATTGTTTGAGCAATTCAACCCAGCCT
TGGTGATTCTTGATTGGAATCTTCCCGATACTAGTGGCTATAGTCTTTGTCAAGAAATGCAGAGCCGTAATAACGTCTTG
GTGATGATCTTGACTGGAAGAACAGAGGAAGCTGACAAAATTAAAATCCTGGCGGCTGGTGCTGATGACTTCATTACTAA
GCCATTTAGTTTGGCTGAAATTGAAGTTCGTGTCCAAGCTCTTTTAAGACGTATCCGCTCTATTAAACCTTCTCCTTCAC
AACGCTTGATCTTTAAGCAGCTAGCAATTAACCCAGAAGGAAGAGAAGTAACTCTGAACGATAAACCTCTGAATCTCACC
GCTTTGGAGTTTAATATTCTCCATTTCTTAGCTAGTCATCCTGGTCAAGCTTGGAGCCGTCCTCAATTGATTCAAAAAAT
TTGGGGTTGTGACTATGTAGGAGATGGACGGGTGGTTGATGTACACATCGGACAGCTACGTAAAAAGTTAGAATCTGATA
CCAGTATACCGGAGTTTATTAAAACTGTTCGCGGTTACGGTTATAAGTTTGAAATACCAGAATCTACACCAATTTAA

Upstream 100 bases:

>100_bases
GCTCATAACACAATCACCTTAACGGCTGTCTCTTGTGTAGGAGCATAACAAACAGCAAAACGCTAGATAAACAATAGGCT
ATAACAATCGCTGAAATAAT

Downstream 100 bases:

>100_bases
GCCAGGAAAACAAGAGTTTACCTGAATAAAAAAGTTCACTCACACATTTCTATCAGGGTGTGGCTATTTATAATTTGACT
GTTTATTTACATCTTCTTTT

Product: two component transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 238; Mature: 238

Protein sequence:

>238_residues
MVMLSATNPKILVVDDDFGVRNLIYRFLSRKYQIESAADGKTALSLFEQFNPALVILDWNLPDTSGYSLCQEMQSRNNVL
VMILTGRTEEADKIKILAAGADDFITKPFSLAEIEVRVQALLRRIRSIKPSPSQRLIFKQLAINPEGREVTLNDKPLNLT
ALEFNILHFLASHPGQAWSRPQLIQKIWGCDYVGDGRVVDVHIGQLRKKLESDTSIPEFIKTVRGYGYKFEIPESTPI

Sequences:

>Translated_238_residues
MVMLSATNPKILVVDDDFGVRNLIYRFLSRKYQIESAADGKTALSLFEQFNPALVILDWNLPDTSGYSLCQEMQSRNNVL
VMILTGRTEEADKIKILAAGADDFITKPFSLAEIEVRVQALLRRIRSIKPSPSQRLIFKQLAINPEGREVTLNDKPLNLT
ALEFNILHFLASHPGQAWSRPQLIQKIWGCDYVGDGRVVDVHIGQLRKKLESDTSIPEFIKTVRGYGYKFEIPESTPI
>Mature_238_residues
MVMLSATNPKILVVDDDFGVRNLIYRFLSRKYQIESAADGKTALSLFEQFNPALVILDWNLPDTSGYSLCQEMQSRNNVL
VMILTGRTEEADKIKILAAGADDFITKPFSLAEIEVRVQALLRRIRSIKPSPSQRLIFKQLAINPEGREVTLNDKPLNLT
ALEFNILHFLASHPGQAWSRPQLIQKIWGCDYVGDGRVVDVHIGQLRKKLESDTSIPEFIKTVRGYGYKFEIPESTPI

Specific function: This Protein Is A Positive Regulator For The Phosphate Regulon. Transcription Of This Operon Is Positively Regulated By Phob And Phor When Phosphate Is Limited. [C]

COG id: COG0745

COG function: function code TK; Response regulators consisting of a CheY-like receiver domain and a winged-helix DNA-binding domain

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1786599, Length=226, Percent_Identity=36.7256637168142, Blast_Score=152, Evalue=1e-38,
Organism=Escherichia coli, GI1789809, Length=235, Percent_Identity=37.4468085106383, Blast_Score=150, Evalue=7e-38,
Organism=Escherichia coli, GI1790863, Length=231, Percent_Identity=35.0649350649351, Blast_Score=136, Evalue=1e-33,
Organism=Escherichia coli, GI1788394, Length=234, Percent_Identity=35.4700854700855, Blast_Score=133, Evalue=9e-33,
Organism=Escherichia coli, GI1790860, Length=223, Percent_Identity=35.8744394618834, Blast_Score=131, Evalue=5e-32,
Organism=Escherichia coli, GI1787375, Length=225, Percent_Identity=33.7777777777778, Blast_Score=130, Evalue=9e-32,
Organism=Escherichia coli, GI1786911, Length=226, Percent_Identity=31.4159292035398, Blast_Score=127, Evalue=5e-31,
Organism=Escherichia coli, GI87082012, Length=223, Percent_Identity=33.1838565022422, Blast_Score=118, Evalue=4e-28,
Organism=Escherichia coli, GI1786784, Length=228, Percent_Identity=29.8245614035088, Blast_Score=116, Evalue=2e-27,
Organism=Escherichia coli, GI145693140, Length=230, Percent_Identity=32.1739130434783, Blast_Score=107, Evalue=5e-25,
Organism=Escherichia coli, GI2367329, Length=225, Percent_Identity=32.8888888888889, Blast_Score=106, Evalue=2e-24,
Organism=Escherichia coli, GI1789402, Length=223, Percent_Identity=31.8385650224215, Blast_Score=105, Evalue=2e-24,
Organism=Escherichia coli, GI1790552, Length=221, Percent_Identity=34.3891402714932, Blast_Score=105, Evalue=3e-24,
Organism=Escherichia coli, GI1787229, Length=207, Percent_Identity=31.8840579710145, Blast_Score=101, Evalue=5e-23,
Organism=Escherichia coli, GI1788550, Length=128, Percent_Identity=32.8125, Blast_Score=70, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001867
- InterPro:   IPR001789
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00072 Response_reg; PF00486 Trans_reg_C [H]

EC number: NA

Molecular weight: Translated: 26918; Mature: 26918

Theoretical pI: Translated: 8.20; Mature: 8.20

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVMLSATNPKILVVDDDFGVRNLIYRFLSRKYQIESAADGKTALSLFEQFNPALVILDWN
CEEEECCCCEEEEEECCCCHHHHHHHHHHHHEEECCCCCCHHHHHHHHHCCCEEEEEECC
LPDTSGYSLCQEMQSRNNVLVMILTGRTEEADKIKILAAGADDFITKPFSLAEIEVRVQA
CCCCCHHHHHHHHHCCCCEEEEEEECCCCCCCEEEEEEECCHHHHCCCCCHHHHHHHHHH
LLRRIRSIKPSPSQRLIFKQLAINPEGREVTLNDKPLNLTALEFNILHFLASHPGQAWSR
HHHHHHCCCCCCHHHHHHHHHHCCCCCCEEEECCCCCEEEEHHHHHHHHHHCCCCCCCCC
PQLIQKIWGCDYVGDGRVVDVHIGQLRKKLESDTSIPEFIKTVRGYGYKFEIPESTPI
HHHHHHHHCCCCCCCCEEEEEEHHHHHHHHHCCCCHHHHHHHHHCCCEEEECCCCCCC
>Mature Secondary Structure
MVMLSATNPKILVVDDDFGVRNLIYRFLSRKYQIESAADGKTALSLFEQFNPALVILDWN
CEEEECCCCEEEEEECCCCHHHHHHHHHHHHEEECCCCCCHHHHHHHHHCCCEEEEEECC
LPDTSGYSLCQEMQSRNNVLVMILTGRTEEADKIKILAAGADDFITKPFSLAEIEVRVQA
CCCCCHHHHHHHHHCCCCEEEEEEECCCCCCCEEEEEEECCHHHHCCCCCHHHHHHHHHH
LLRRIRSIKPSPSQRLIFKQLAINPEGREVTLNDKPLNLTALEFNILHFLASHPGQAWSR
HHHHHHCCCCCCHHHHHHHHHHCCCCCCEEEECCCCCEEEEHHHHHHHHHHCCCCCCCCC
PQLIQKIWGCDYVGDGRVVDVHIGQLRKKLESDTSIPEFIKTVRGYGYKFEIPESTPI
HHHHHHHHCCCCCCCCEEEEEEHHHHHHHHHCCCCHHHHHHHHHCCCEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1554697 [H]