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The map label for this gene is gshB [H]

Identifier: 75908057

GI number: 75908057

Start: 2282935

End: 2283909

Strand: Direct

Name: gshB [H]

Synonym: Ava_1836

Alternate gene names: 75908057

Gene position: 2282935-2283909 (Clockwise)

Preceding gene: 75908056

Following gene: 75908061

Centisome position: 35.86

GC content: 43.79

Gene sequence:

>975_bases
GTGAAACTGGCTTTTATTATAGATCCCATCCATCAGTTAGACCCATGCCATGATACCAGCGTGGCGCTGATGGAAGCAGC
GCAAATTTTAGGACATGAAGTTTGGGTAACTCAGGCTAATTGGTTGAGTGTAGTAGACAGCAAAGCTTGGGCTATATTGC
AGCAGGTAGAACTTGTACCAGTGCAGTTGGTAGATGGACGATGGGTTGCAGCAAATCCTTGGTATAAATTAAATGCTCGT
TCCCTCAATTCTTTGGAAACGATGGATGCTGTATTTATGCGGACAGATCCACCCGTAAACGATGCCTATCTTTACGCTAC
CTATGTTCTGGATTACGTTGACCAGCAGAAAACTTTGGTCATTAACAATCCCAATGGTATCCGTAAGGCAAATGAAAAAA
TGTATGCCCTGCAATTTACCAAGGCAATTCCCGAAACCATCGTCAGTGCAGATAAAGATTTTATCCGGCAATTTGTCGAA
GCAAAAGGCGCTACTATCCTCAAACCCTTGGGTAACAAAGCTGGGGAAGGGATTTTATTTCTACAAGCAGGCGATCGCAA
TTTTAACTCTATCGTCGAACTCAGCACCCAGCAAGGCAGATTACCAGTAATGGTACAAACTTATCTATCAGAGGCCAAAG
AGGGAGATAAGCGGATCATTTTACTTAATGGTGAACCCATTGGCGCTCTCAACCGTTTGGCAAGTGGCAGTGATTTTCGT
AATAACATGGCCACTGGTGGAACGGTCGCAAAAACCGAAATTACCCCACGAGAACAAGAAATTTGCGGCGATTTGGCTGC
AAATTTACGCCAAGATGGGTTAGTCTTTGTGGGTATTGATGTGATTGGTGGCTATCTCACAGAAGTTAACGTCACTAGCC
CAACTGGGATACGGGAAATCGACCGACTAGATGGTACTCGCCTGGCTAATCAAGTAATTCAATGGGTGGAAAAAAACCTA
CAAATTCAAAATTAA

Upstream 100 bases:

>100_bases
AGCAATAGGATTACGCCTTTTTCCTCAATAGTATTTGTCAATCTGTCAGGATATCGGGGGATAATTTATCTGAGAATTTC
CTGGCGTTGAGGCAAAAGGC

Downstream 100 bases:

>100_bases
AGACAGGTAATTACAGGAAAACTACATGAAGAGGACGTGAACATTTCTCTTTTCAGTCTCCCTCCTGTATATCACAAGTA
CACACTTGGGCTTAAAAGTC

Product: glutathione synthetase

Products: NA

Alternate protein names: GSH synthetase; GSH-S; GSHase; Glutathione synthase [H]

Number of amino acids: Translated: 324; Mature: 324

Protein sequence:

>324_residues
MKLAFIIDPIHQLDPCHDTSVALMEAAQILGHEVWVTQANWLSVVDSKAWAILQQVELVPVQLVDGRWVAANPWYKLNAR
SLNSLETMDAVFMRTDPPVNDAYLYATYVLDYVDQQKTLVINNPNGIRKANEKMYALQFTKAIPETIVSADKDFIRQFVE
AKGATILKPLGNKAGEGILFLQAGDRNFNSIVELSTQQGRLPVMVQTYLSEAKEGDKRIILLNGEPIGALNRLASGSDFR
NNMATGGTVAKTEITPREQEICGDLAANLRQDGLVFVGIDVIGGYLTEVNVTSPTGIREIDRLDGTRLANQVIQWVEKNL
QIQN

Sequences:

>Translated_324_residues
MKLAFIIDPIHQLDPCHDTSVALMEAAQILGHEVWVTQANWLSVVDSKAWAILQQVELVPVQLVDGRWVAANPWYKLNAR
SLNSLETMDAVFMRTDPPVNDAYLYATYVLDYVDQQKTLVINNPNGIRKANEKMYALQFTKAIPETIVSADKDFIRQFVE
AKGATILKPLGNKAGEGILFLQAGDRNFNSIVELSTQQGRLPVMVQTYLSEAKEGDKRIILLNGEPIGALNRLASGSDFR
NNMATGGTVAKTEITPREQEICGDLAANLRQDGLVFVGIDVIGGYLTEVNVTSPTGIREIDRLDGTRLANQVIQWVEKNL
QIQN
>Mature_324_residues
MKLAFIIDPIHQLDPCHDTSVALMEAAQILGHEVWVTQANWLSVVDSKAWAILQQVELVPVQLVDGRWVAANPWYKLNAR
SLNSLETMDAVFMRTDPPVNDAYLYATYVLDYVDQQKTLVINNPNGIRKANEKMYALQFTKAIPETIVSADKDFIRQFVE
AKGATILKPLGNKAGEGILFLQAGDRNFNSIVELSTQQGRLPVMVQTYLSEAKEGDKRIILLNGEPIGALNRLASGSDFR
NNMATGGTVAKTEITPREQEICGDLAANLRQDGLVFVGIDVIGGYLTEVNVTSPTGIREIDRLDGTRLANQVIQWVEKNL
QIQN

Specific function: Glutathione biosynthesis; second step. [C]

COG id: COG0189

COG function: function code HJ; Glutathione synthase/Ribosomal protein S6 modification enzyme (glutaminyl transferase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Escherichia coli, GI1789316, Length=326, Percent_Identity=33.1288343558282, Blast_Score=196, Evalue=1e-51,
Organism=Escherichia coli, GI1787076, Length=248, Percent_Identity=25.4032258064516, Blast_Score=75, Evalue=6e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR006284
- InterPro:   IPR004218
- InterPro:   IPR004215
- InterPro:   IPR013817
- InterPro:   IPR016185 [H]

Pfam domain/function: PF02955 GSH-S_ATP; PF02951 GSH-S_N [H]

EC number: =6.3.2.3 [H]

Molecular weight: Translated: 35923; Mature: 35923

Theoretical pI: Translated: 4.82; Mature: 4.82

Prosite motif: PS50975 ATP_GRASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLAFIIDPIHQLDPCHDTSVALMEAAQILGHEVWVTQANWLSVVDSKAWAILQQVELVP
CEEEEEECCHHHCCCCCCCHHHHHHHHHHHCCEEEEECCHHHHHHHHHHHHHHHHHHEEE
VQLVDGRWVAANPWYKLNARSLNSLETMDAVFMRTDPPVNDAYLYATYVLDYVDQQKTLV
EEEECCEEEECCCCEEECCCCCCHHHHHHHHEEECCCCCCCHHHHHHHHHHHHCCCCEEE
INNPNGIRKANEKMYALQFTKAIPETIVSADKDFIRQFVEAKGATILKPLGNKAGEGILF
EECCCCCHHHCCCEEEEHHHHHHHHHHHHCCHHHHHHHHHHCCCEEEECCCCCCCCEEEE
LQAGDRNFNSIVELSTQQGRLPVMVQTYLSEAKEGDKRIILLNGEPIGALNRLASGSDFR
EEECCCCCCHHEEECCCCCCCCHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHCCCCCHH
NNMATGGTVAKTEITPREQEICGDLAANLRQDGLVFVGIDVIGGYLTEVNVTSPTGIREI
CCCCCCCEEEEEECCCCHHHHHHHHHHHHHHCCEEEEEHHHHCCEEEEEECCCCCCHHHH
DRLDGTRLANQVIQWVEKNLQIQN
HHCCHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MKLAFIIDPIHQLDPCHDTSVALMEAAQILGHEVWVTQANWLSVVDSKAWAILQQVELVP
CEEEEEECCHHHCCCCCCCHHHHHHHHHHHCCEEEEECCHHHHHHHHHHHHHHHHHHEEE
VQLVDGRWVAANPWYKLNARSLNSLETMDAVFMRTDPPVNDAYLYATYVLDYVDQQKTLV
EEEECCEEEECCCCEEECCCCCCHHHHHHHHEEECCCCCCCHHHHHHHHHHHHCCCCEEE
INNPNGIRKANEKMYALQFTKAIPETIVSADKDFIRQFVEAKGATILKPLGNKAGEGILF
EECCCCCHHHCCCEEEEHHHHHHHHHHHHCCHHHHHHHHHHCCCEEEECCCCCCCCEEEE
LQAGDRNFNSIVELSTQQGRLPVMVQTYLSEAKEGDKRIILLNGEPIGALNRLASGSDFR
EEECCCCCCHHEEECCCCCCCCHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHCCCCCHH
NNMATGGTVAKTEITPREQEICGDLAANLRQDGLVFVGIDVIGGYLTEVNVTSPTGIREI
CCCCCCCEEEEEECCCCHHHHHHHHHHHHHHCCEEEEEHHHHCCEEEEEECCCCCCHHHH
DRLDGTRLANQVIQWVEKNLQIQN
HHCCHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7557485; 11759840; 8525061 [H]