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The map label for this gene is moaA

Identifier: 75908051

GI number: 75908051

Start: 2276596

End: 2277582

Strand: Direct

Name: moaA

Synonym: Ava_1830

Alternate gene names: 75908051

Gene position: 2276596-2277582 (Clockwise)

Preceding gene: 75908048

Following gene: 75908052

Centisome position: 35.76

GC content: 45.9

Gene sequence:

>987_bases
ATGAACCAGGTAGACTACCTCCGCATTAGCTTAATAGATCGCTGCAATTTCCGTTGTCAGTACTGTATGCCAGAGGGGTC
AGAGTTAGACTATATCCTCAAGCAACAATTATTGACTGATGAGGAATTGCTCACTTTAGTTCAAGAAGTATTTATTCCTG
TTGGTTTTAGACAATTTCGCTTAACTGGGGGAGAACCTTTATTACGCCCCCGTGTTGTGGATTTGGTCGGGGCGATCGCT
TCTTTACCCCAAACCCAAGACCTCTCCATGACGACTAACGGCTTTTTACTCGCACCCATCGCCCAAAATCTCTACGATGC
TGGTTTACGGCGCATCAATATCAGCTTAGATTCCCTCGATCCCCATATATTTGACCAGATTATCGGCAGTCATGGACGTT
CTCGCTGGCAACAAGTTTGGGATGGTATTCAAGCTGCCCATCGTGTAGGCTTCGATCCATTGAAACTAAATGTAGTGGTA
ATTCCTGGTGTCAATGACCACGAAATTCTCGATTTAGCCGCCTTAACAATTGATAAACAATGGCACGTCCGGTTTATTGA
ATTTATGCCCATTGGTAACGGAGAGTTATTTGGCGATCGCGGTTGGGTATCTTCATCTCAATTACGCCAACAAATCCGCG
ATCGCTGGGGCTTGACAGATGCTCAAGTTCGTGGTTCTGGCCCGGCGGATGTCTTCCAAATCCCTGGAGCCAAAGGCACA
CTAGGATTTATCAGCCAAATGTCAGAATGTTTTTGCGATCGTTGTAACCGGATGCGTCTCAGTGCCGATGGCTGGTTACG
CCCTTGCTTATTAAACGAAACAGGTCAACTCGATCTAAAAACATCTCTACGCTCTGGTGTGAGTATCCACCAGTTACGAG
AACAGGTGAGACATTTATTAGCAATCAAGCCAGAAATTAACTATAAAGGGCGTGATTCAGGTACTACAGGAGCCTACAGC
CGCACAATGTCACAAATCGGAGGGTAA

Upstream 100 bases:

>100_bases
CATGCTTACATTTTACTAGACATTCTGTAAGAAATCCAAATTAATTAAATCCGTGCTACAAGCACGGGGTTTTAAACCCA
TTTTTCTGATAAAACCCACA

Downstream 100 bases:

>100_bases
GCAGAGATAATTTGATTAAACAGGAGAGATGATCATGGTCAACAGCCTTAAAGAACTAATTGATGAGCCAGAGTTGGGTC
ATAATGACGACCCAGAGGAA

Product: GTP cyclohydrolase subunit MoaA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 328; Mature: 328

Protein sequence:

>328_residues
MNQVDYLRISLIDRCNFRCQYCMPEGSELDYILKQQLLTDEELLTLVQEVFIPVGFRQFRLTGGEPLLRPRVVDLVGAIA
SLPQTQDLSMTTNGFLLAPIAQNLYDAGLRRINISLDSLDPHIFDQIIGSHGRSRWQQVWDGIQAAHRVGFDPLKLNVVV
IPGVNDHEILDLAALTIDKQWHVRFIEFMPIGNGELFGDRGWVSSSQLRQQIRDRWGLTDAQVRGSGPADVFQIPGAKGT
LGFISQMSECFCDRCNRMRLSADGWLRPCLLNETGQLDLKTSLRSGVSIHQLREQVRHLLAIKPEINYKGRDSGTTGAYS
RTMSQIGG

Sequences:

>Translated_328_residues
MNQVDYLRISLIDRCNFRCQYCMPEGSELDYILKQQLLTDEELLTLVQEVFIPVGFRQFRLTGGEPLLRPRVVDLVGAIA
SLPQTQDLSMTTNGFLLAPIAQNLYDAGLRRINISLDSLDPHIFDQIIGSHGRSRWQQVWDGIQAAHRVGFDPLKLNVVV
IPGVNDHEILDLAALTIDKQWHVRFIEFMPIGNGELFGDRGWVSSSQLRQQIRDRWGLTDAQVRGSGPADVFQIPGAKGT
LGFISQMSECFCDRCNRMRLSADGWLRPCLLNETGQLDLKTSLRSGVSIHQLREQVRHLLAIKPEINYKGRDSGTTGAYS
RTMSQIGG
>Mature_328_residues
MNQVDYLRISLIDRCNFRCQYCMPEGSELDYILKQQLLTDEELLTLVQEVFIPVGFRQFRLTGGEPLLRPRVVDLVGAIA
SLPQTQDLSMTTNGFLLAPIAQNLYDAGLRRINISLDSLDPHIFDQIIGSHGRSRWQQVWDGIQAAHRVGFDPLKLNVVV
IPGVNDHEILDLAALTIDKQWHVRFIEFMPIGNGELFGDRGWVSSSQLRQQIRDRWGLTDAQVRGSGPADVFQIPGAKGT
LGFISQMSECFCDRCNRMRLSADGWLRPCLLNETGQLDLKTSLRSGVSIHQLREQVRHLLAIKPEINYKGRDSGTTGAYS
RTMSQIGG

Specific function: Together with moaC, is involved in the conversion of a guanosine derivative (5'-GTP) into molybdopterin precursor Z

COG id: COG2896

COG function: function code H; Molybdenum cofactor biosynthesis enzyme

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. MoaA family

Homologues:

Organism=Homo sapiens, GI116734668, Length=291, Percent_Identity=43.298969072165, Blast_Score=225, Evalue=5e-59,
Organism=Homo sapiens, GI22027536, Length=291, Percent_Identity=43.298969072165, Blast_Score=224, Evalue=1e-58,
Organism=Escherichia coli, GI1786999, Length=323, Percent_Identity=33.7461300309598, Blast_Score=175, Evalue=3e-45,
Organism=Caenorhabditis elegans, GI32566329, Length=337, Percent_Identity=39.4658753709199, Blast_Score=210, Evalue=9e-55,
Organism=Caenorhabditis elegans, GI32566331, Length=303, Percent_Identity=40.9240924092409, Blast_Score=209, Evalue=1e-54,
Organism=Caenorhabditis elegans, GI17561210, Length=336, Percent_Identity=39.5833333333333, Blast_Score=207, Evalue=6e-54,
Organism=Drosophila melanogaster, GI28574490, Length=292, Percent_Identity=40.7534246575342, Blast_Score=196, Evalue=2e-50,
Organism=Drosophila melanogaster, GI28574488, Length=292, Percent_Identity=40.7534246575342, Blast_Score=195, Evalue=3e-50,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MOAA_ANAVT (Q3MC34)

Other databases:

- EMBL:   CP000117
- RefSeq:   YP_322347.1
- HSSP:   P69848
- ProteinModelPortal:   Q3MC34
- STRING:   Q3MC34
- GeneID:   3681938
- GenomeReviews:   CP000117_GR
- KEGG:   ava:Ava_1830
- NMPDR:   fig|240292.3.peg.2910
- eggNOG:   COG2896
- HOGENOM:   HBG529885
- OMA:   NESEVED
- PhylomeDB:   Q3MC34
- ProtClustDB:   CLSK896062
- BioCyc:   AVAR240292:AVA_1830-MONOMER
- HAMAP:   MF_01225_B
- InterPro:   IPR013785
- InterPro:   IPR006638
- InterPro:   IPR013483
- InterPro:   IPR000385
- InterPro:   IPR010505
- InterPro:   IPR007197
- Gene3D:   G3DSA:3.20.20.70
- SMART:   SM00729
- TIGRFAMs:   TIGR02666

Pfam domain/function: PF06463 Mob_synth_C; PF04055 Radical_SAM

EC number: NA

Molecular weight: Translated: 36906; Mature: 36906

Theoretical pI: Translated: 6.86; Mature: 6.86

Prosite motif: PS01305 MOAA_NIFB_PQQE

Important sites: BINDING 8-8 BINDING 21-21 BINDING 60-60 BINDING 64-64 BINDING 91-91 BINDING 115-115 BINDING 155-155 BINDING 189-189

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNQVDYLRISLIDRCNFRCQYCMPEGSELDYILKQQLLTDEELLTLVQEVFIPVGFRQFR
CCCCCEEEEEEHHCCCCEEEEECCCCCCHHHHHHHHHCCHHHHHHHHHHHHHCCCCEEEE
LTGGEPLLRPRVVDLVGAIASLPQTQDLSMTTNGFLLAPIAQNLYDAGLRRINISLDSLD
ECCCCCCCCHHHHHHHHHHHHCCCCCCCEECCCCEEEHHHHHHHHHCCCEEEEEEHHCCC
PHIFDQIIGSHGRSRWQQVWDGIQAAHRVGFDPLKLNVVVIPGVNDHEILDLAALTIDKQ
HHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCHHHHHHHHEECCC
WHVRFIEFMPIGNGELFGDRGWVSSSQLRQQIRDRWGLTDAQVRGSGPADVFQIPGAKGT
CEEEEEEEEECCCCCEECCCCCCCHHHHHHHHHHHHCCCCCEECCCCCCCEEECCCCCCH
LGFISQMSECFCDRCNRMRLSADGWLRPCLLNETGQLDLKTSLRSGVSIHQLREQVRHLL
HHHHHHHHHHHHHHHCCEEECCCCCCCCEECCCCCCEEEHHHHHCCCHHHHHHHHHHHHH
AIKPEINYKGRDSGTTGAYSRTMSQIGG
CCCCCCCCCCCCCCCCHHHHHHHHHHCC
>Mature Secondary Structure
MNQVDYLRISLIDRCNFRCQYCMPEGSELDYILKQQLLTDEELLTLVQEVFIPVGFRQFR
CCCCCEEEEEEHHCCCCEEEEECCCCCCHHHHHHHHHCCHHHHHHHHHHHHHCCCCEEEE
LTGGEPLLRPRVVDLVGAIASLPQTQDLSMTTNGFLLAPIAQNLYDAGLRRINISLDSLD
ECCCCCCCCHHHHHHHHHHHHCCCCCCCEECCCCEEEHHHHHHHHHCCCEEEEEEHHCCC
PHIFDQIIGSHGRSRWQQVWDGIQAAHRVGFDPLKLNVVVIPGVNDHEILDLAALTIDKQ
HHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCHHHHHHHHEECCC
WHVRFIEFMPIGNGELFGDRGWVSSSQLRQQIRDRWGLTDAQVRGSGPADVFQIPGAKGT
CEEEEEEEEECCCCCEECCCCCCCHHHHHHHHHHHHCCCCCEECCCCCCCEEECCCCCCH
LGFISQMSECFCDRCNRMRLSADGWLRPCLLNETGQLDLKTSLRSGVSIHQLREQVRHLL
HHHHHHHHHHHHHHHCCEEECCCCCCCCEECCCCCCEEEHHHHHCCCHHHHHHHHHHHHH
AIKPEINYKGRDSGTTGAYSRTMSQIGG
CCCCCCCCCCCCCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA