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The map label for this gene is ybiK [C]

Identifier: 75907996

GI number: 75907996

Start: 2213465

End: 2214421

Strand: Direct

Name: ybiK [C]

Synonym: Ava_1775

Alternate gene names: 75907996

Gene position: 2213465-2214421 (Clockwise)

Preceding gene: 75907995

Following gene: 75908000

Centisome position: 34.77

GC content: 46.5

Gene sequence:

>957_bases
ATGAAGTCACAGGTGCAACCAAAATTAATTATTCATGGGGGAGCAGGTAGTTCTCTCCACGGCAAAGGTGGATTAGAAGC
AGTGCGCCAAACACTCCATGCAATAGTAGAGGAAGTCTACACCCTATTATTGTCAGGAGCGAATGCTTCTGTCGCAGTGG
TGCGGGGTTGTCAACTGTTGGAAGATGAACCCCGCTTCAATGCTGGTACTGGTTCGGTGCTGCAATCTGATGGGCAAATC
CGTATGAGTGCTTCCATCATGGATGGTGCATTAGGACGCTTTAGTGGTGTAATAAATGTTTCACGGGTAAAAAATCCGAT
TGAGTTAGCACAATTTTTACAAAATTCCCCAGACCGAGTGCTGTCAGATTACGGCTCGGCTGAATTAGCCAGGGAAATGC
AAATTCCTAGCTATAACGCTTTAACTGAGCTGCGATTACAAGAATGGATACAAGAAAGACAAGATAATTTCAAAAGAACA
ATGGCTGGGGTAATAGCCGAACCAGAACTTTTGGAAACTAGTAATGCCGGACGTGGCACAATCGGCGTAGTAGCTTTAGA
TACCTATGGTAAACTAGCAGTTGGTACATCTACAGGTGGCAAAGGCTTTGAGCGCATTGGACGCGTCAGTGATTCTGCTA
TGCCAGCAGGTAATTATGCTACTAGTTATGCCGCCGTCAGCTGTACAGGCATCGGCGAAGATATTATTGATGAGTGCCTA
GCCCCCAAGATTGTCATTCGTGTCACAGATGGATTGTCTCTGCAAGAGTCTATGCAGCGCTCCTTTGCCGAAGCACACGA
AAATAAAAGAGATTTCGGAGCGATCGCCTTAGATGCCACCGGAGCGATCTCCTGGGGTAAAACTTGTGACATTATCCTCG
CCGCCTTTCATGATGGCGAAAAAATTGGGGATACATTAGAACTCCCAGGTGGTACCCAGGTGGGTAGTATCAGTTAG

Upstream 100 bases:

>100_bases
CGCCGCCGTCGTTCTGAGGCTAAAACTGGGGACTGAATCAGTTAACCGTCAACTGATACACCCAATATTCAAACTAAAAC
GCAAATAGTGCAGGGTATAA

Downstream 100 bases:

>100_bases
GGATTGGGGACAAAAAAGTAAAAAGTAAAAAGGTTTTTTCTTTTGACTTTTGCCTTTTGACTTTTGCCTTGAATTGGGGA
CTTCTCCCCCTGCTCCCTGC

Product: asparaginase

Products: NA

Alternate protein names: Beta-aspartyl-peptidase; Isoaspartyl dipeptidase [H]

Number of amino acids: Translated: 318; Mature: 318

Protein sequence:

>318_residues
MKSQVQPKLIIHGGAGSSLHGKGGLEAVRQTLHAIVEEVYTLLLSGANASVAVVRGCQLLEDEPRFNAGTGSVLQSDGQI
RMSASIMDGALGRFSGVINVSRVKNPIELAQFLQNSPDRVLSDYGSAELAREMQIPSYNALTELRLQEWIQERQDNFKRT
MAGVIAEPELLETSNAGRGTIGVVALDTYGKLAVGTSTGGKGFERIGRVSDSAMPAGNYATSYAAVSCTGIGEDIIDECL
APKIVIRVTDGLSLQESMQRSFAEAHENKRDFGAIALDATGAISWGKTCDIILAAFHDGEKIGDTLELPGGTQVGSIS

Sequences:

>Translated_318_residues
MKSQVQPKLIIHGGAGSSLHGKGGLEAVRQTLHAIVEEVYTLLLSGANASVAVVRGCQLLEDEPRFNAGTGSVLQSDGQI
RMSASIMDGALGRFSGVINVSRVKNPIELAQFLQNSPDRVLSDYGSAELAREMQIPSYNALTELRLQEWIQERQDNFKRT
MAGVIAEPELLETSNAGRGTIGVVALDTYGKLAVGTSTGGKGFERIGRVSDSAMPAGNYATSYAAVSCTGIGEDIIDECL
APKIVIRVTDGLSLQESMQRSFAEAHENKRDFGAIALDATGAISWGKTCDIILAAFHDGEKIGDTLELPGGTQVGSIS
>Mature_318_residues
MKSQVQPKLIIHGGAGSSLHGKGGLEAVRQTLHAIVEEVYTLLLSGANASVAVVRGCQLLEDEPRFNAGTGSVLQSDGQI
RMSASIMDGALGRFSGVINVSRVKNPIELAQFLQNSPDRVLSDYGSAELAREMQIPSYNALTELRLQEWIQERQDNFKRT
MAGVIAEPELLETSNAGRGTIGVVALDTYGKLAVGTSTGGKGFERIGRVSDSAMPAGNYATSYAAVSCTGIGEDIIDECL
APKIVIRVTDGLSLQESMQRSFAEAHENKRDFGAIALDATGAISWGKTCDIILAAFHDGEKIGDTLELPGGTQVGSIS

Specific function: Degrades proteins damaged by L-isoaspartyl residue formation (also known as beta-Asp residues). Probably performs the final step in the degradation of the reserve polymer cyanophycin (depolymerizes the building block L-beta-Asp-Arg). Also has L- asparagin

COG id: COG1446

COG function: function code E; Asparaginase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Ntn-hydrolase family [H]

Homologues:

Organism=Homo sapiens, GI145275202, Length=302, Percent_Identity=33.4437086092715, Blast_Score=119, Evalue=3e-27,
Organism=Homo sapiens, GI145275200, Length=302, Percent_Identity=33.4437086092715, Blast_Score=119, Evalue=3e-27,
Organism=Escherichia coli, GI1787050, Length=267, Percent_Identity=34.8314606741573, Blast_Score=102, Evalue=5e-23,
Organism=Drosophila melanogaster, GI18921183, Length=309, Percent_Identity=30.0970873786408, Blast_Score=78, Evalue=9e-15,
Organism=Drosophila melanogaster, GI21357893, Length=282, Percent_Identity=28.0141843971631, Blast_Score=73, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000246 [H]

Pfam domain/function: PF01112 Asparaginase_2 [H]

EC number: =3.4.19.5 [H]

Molecular weight: Translated: 33669; Mature: 33669

Theoretical pI: Translated: 4.84; Mature: 4.84

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSQVQPKLIIHGGAGSSLHGKGGLEAVRQTLHAIVEEVYTLLLSGANASVAVVRGCQLL
CCCCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHH
EDEPRFNAGTGSVLQSDGQIRMSASIMDGALGRFSGVINVSRVKNPIELAQFLQNSPDRV
CCCCCCCCCCCCCEECCCCEEEEHHHHHHHHHHHHCCEEHHHCCCHHHHHHHHHCCHHHH
LSDYGSAELAREMQIPSYNALTELRLQEWIQERQDNFKRTMAGVIAEPELLETSNAGRGT
HHHCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCCCE
IGVVALDTYGKLAVGTSTGGKGFERIGRVSDSAMPAGNYATSYAAVSCTGIGEDIIDECL
EEEEEEECCCCEEEECCCCCHHHHHHHCCCCCCCCCCCCHHCEEEEEECCCCHHHHHHHC
APKIVIRVTDGLSLQESMQRSFAEAHENKRDFGAIALDATGAISWGKTCDIILAAFHDGE
CCEEEEEECCCCCHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCEEEEEEEECCCC
KIGDTLELPGGTQVGSIS
CCCCEEECCCCCCCCCCC
>Mature Secondary Structure
MKSQVQPKLIIHGGAGSSLHGKGGLEAVRQTLHAIVEEVYTLLLSGANASVAVVRGCQLL
CCCCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHH
EDEPRFNAGTGSVLQSDGQIRMSASIMDGALGRFSGVINVSRVKNPIELAQFLQNSPDRV
CCCCCCCCCCCCCEECCCCEEEEHHHHHHHHHHHHCCEEHHHCCCHHHHHHHHHCCHHHH
LSDYGSAELAREMQIPSYNALTELRLQEWIQERQDNFKRTMAGVIAEPELLETSNAGRGT
HHHCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCCCE
IGVVALDTYGKLAVGTSTGGKGFERIGRVSDSAMPAGNYATSYAAVSCTGIGEDIIDECL
EEEEEEECCCCEEEECCCCCHHHHHHHCCCCCCCCCCCCHHCEEEEEECCCCHHHHHHHC
APKIVIRVTDGLSLQESMQRSFAEAHENKRDFGAIALDATGAISWGKTCDIILAAFHDGE
CCEEEEEECCCCCHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCEEEEEEEECCCC
KIGDTLELPGGTQVGSIS
CCCCEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11759840 [H]