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The map label for this gene is ispH [H]

Identifier: 75676451

GI number: 75676451

Start: 2469319

End: 2470263

Strand: Direct

Name: ispH [H]

Synonym: Nwi_2266

Alternate gene names: 75676451

Gene position: 2469319-2470263 (Clockwise)

Preceding gene: 75676449

Following gene: 75676452

Centisome position: 72.58

GC content: 60.21

Gene sequence:

>945_bases
TTGACTGATCCTCGTCCGATTGAAGTTCTGCTGGCACAGCCGCGCGGATTCTGCGCAGGCGTTGTCCGCGCGATCGAGAC
TGTCGAACGCGCGCTGGAGAAGTACGGTCCCCCAGTGTATGTCCGCCACGAGATCGTACACAACAAATACGTCGTCGAAA
GCCTGAAGAATAAAGGGGCGATTTTCGTCGAGGATCTGTCGGAGGTCCCTCCAAAGGCGGTGACCGTATTCAGTGCTCAC
GGCGTGGCGCAAAGCGTGGAAGAAGAGGCCGCCGCTCGCGGTCTTCCCGTGCTCAACGCCACCTGCCCGCTCGTCACCAA
GGTTCACAACCAGGGCAAACGCTACACGTCCAAGGGCCGGACCCTGATCCTGATCGGCCATGCCGGCCATCCCGAGGTCG
AGGGAACTATGGGACAGGTTCCAGGACCCGTCCTGCTTGTTCAGAATGTCGACGACGTCGCGGCCCTGACGCTGCCCGCC
GATACGCCCGTGGCCTATATCACGCAGACGACGCTGAGCGTCGACGACACCAAGGACATCATCATCGCCCTCCAGCAGCG
TTTTACTGATATTCAAGGTCCCGATACGCGGGATATCTGCTATGCAACGCAGAATCGCCAATCCGCGGTAAGAGACCTGA
GCAAGCTGGTGGACGTCATTCTGGTGGTCGGCGCAGCCAATAGTTCCAATTCAAACCGGCTGCGGGAAATTGGCACCGAG
GTCGGCGTCGCCAGTTATCTTATCGCTGACGGCAGCGAACTCAACGCTGAGTGGCTGGAAGGGGCGAAAGCCGTGGGCGT
TACCGCGGGCGCTTCGGCTCCGGAAGTCCTGGTCGACGACGTCATCGAGGCCTTGAAGCGCATCAGGCCGGTCGCTGTCT
CGGTCCTGCCGGGACGCGAAGAGAACATTGAATTCCGGCTTCCCTCTGAACTCACCTCAGCTTGA

Upstream 100 bases:

>100_bases
TCGCAGATTATACGTCTGTGACATTTCTTGAAGGCGCGCGCGATTTTGAGAAGACCGTCCGGTTCTATATGGAGTTACAG
CATAATGCATAAGGTGCGAA

Downstream 100 bases:

>100_bases
TCCCCTTCAGATTTTCAGCTGTTAACTCCCGAAAGAACACTCGATTATGGCAATACCGTTTTTCAAAGAACTGAAGATCA
GCGGGTATCTGATCAAGCAG

Product: 4-hydroxy-3-methylbut-2-enyl diphosphate reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 314; Mature: 313

Protein sequence:

>314_residues
MTDPRPIEVLLAQPRGFCAGVVRAIETVERALEKYGPPVYVRHEIVHNKYVVESLKNKGAIFVEDLSEVPPKAVTVFSAH
GVAQSVEEEAAARGLPVLNATCPLVTKVHNQGKRYTSKGRTLILIGHAGHPEVEGTMGQVPGPVLLVQNVDDVAALTLPA
DTPVAYITQTTLSVDDTKDIIIALQQRFTDIQGPDTRDICYATQNRQSAVRDLSKLVDVILVVGAANSSNSNRLREIGTE
VGVASYLIADGSELNAEWLEGAKAVGVTAGASAPEVLVDDVIEALKRIRPVAVSVLPGREENIEFRLPSELTSA

Sequences:

>Translated_314_residues
MTDPRPIEVLLAQPRGFCAGVVRAIETVERALEKYGPPVYVRHEIVHNKYVVESLKNKGAIFVEDLSEVPPKAVTVFSAH
GVAQSVEEEAAARGLPVLNATCPLVTKVHNQGKRYTSKGRTLILIGHAGHPEVEGTMGQVPGPVLLVQNVDDVAALTLPA
DTPVAYITQTTLSVDDTKDIIIALQQRFTDIQGPDTRDICYATQNRQSAVRDLSKLVDVILVVGAANSSNSNRLREIGTE
VGVASYLIADGSELNAEWLEGAKAVGVTAGASAPEVLVDDVIEALKRIRPVAVSVLPGREENIEFRLPSELTSA
>Mature_313_residues
TDPRPIEVLLAQPRGFCAGVVRAIETVERALEKYGPPVYVRHEIVHNKYVVESLKNKGAIFVEDLSEVPPKAVTVFSAHG
VAQSVEEEAAARGLPVLNATCPLVTKVHNQGKRYTSKGRTLILIGHAGHPEVEGTMGQVPGPVLLVQNVDDVAALTLPAD
TPVAYITQTTLSVDDTKDIIIALQQRFTDIQGPDTRDICYATQNRQSAVRDLSKLVDVILVVGAANSSNSNRLREIGTEV
GVASYLIADGSELNAEWLEGAKAVGVTAGASAPEVLVDDVIEALKRIRPVAVSVLPGREENIEFRLPSELTSA

Specific function: Converts 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate into isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP) [H]

COG id: COG0761

COG function: function code IM; Penicillin tolerance protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispH family [H]

Homologues:

Organism=Escherichia coli, GI1786212, Length=308, Percent_Identity=55.8441558441558, Blast_Score=348, Evalue=2e-97,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003451 [H]

Pfam domain/function: PF02401 LYTB [H]

EC number: =1.17.1.2 [H]

Molecular weight: Translated: 33650; Mature: 33519

Theoretical pI: Translated: 4.97; Mature: 4.97

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
0.3 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDPRPIEVLLAQPRGFCAGVVRAIETVERALEKYGPPVYVRHEIVHNKYVVESLKNKGA
CCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHCCCC
IFVEDLSEVPPKAVTVFSAHGVAQSVEEEAAARGLPVLNATCPLVTKVHNQGKRYTSKGR
EEEECHHHCCCCEEEEEECCCHHHHHHHHHHHCCCCEECCCCHHHHHHHHCCCCCCCCCC
TLILIGHAGHPEVEGTMGQVPGPVLLVQNVDDVAALTLPADTPVAYITQTTLSVDDTKDI
EEEEEECCCCCCCCCCCCCCCCCEEEEECCCCEEEEEECCCCCEEEEEEEEECCCCHHHH
IIALQQRFTDIQGPDTRDICYATQNRQSAVRDLSKLVDVILVVGAANSSNSNRLREIGTE
HHHHHHHHCCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHEEEECCCCCCCHHHHHHHHH
VGVASYLIADGSELNAEWLEGAKAVGVTAGASAPEVLVDDVIEALKRIRPVAVSVLPGRE
HHHHHHEEECCCCCCHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHCCCCEEEEECCCC
ENIEFRLPSELTSA
CCEEEECCHHHCCC
>Mature Secondary Structure 
TDPRPIEVLLAQPRGFCAGVVRAIETVERALEKYGPPVYVRHEIVHNKYVVESLKNKGA
CCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHCCCC
IFVEDLSEVPPKAVTVFSAHGVAQSVEEEAAARGLPVLNATCPLVTKVHNQGKRYTSKGR
EEEECHHHCCCCEEEEEECCCHHHHHHHHHHHCCCCEECCCCHHHHHHHHCCCCCCCCCC
TLILIGHAGHPEVEGTMGQVPGPVLLVQNVDDVAALTLPADTPVAYITQTTLSVDDTKDI
EEEEEECCCCCCCCCCCCCCCCCEEEEECCCCEEEEEECCCCCEEEEEEEEECCCCHHHH
IIALQQRFTDIQGPDTRDICYATQNRQSAVRDLSKLVDVILVVGAANSSNSNRLREIGTE
HHHHHHHHCCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHEEEECCCCCCCHHHHHHHHH
VGVASYLIADGSELNAEWLEGAKAVGVTAGASAPEVLVDDVIEALKRIRPVAVSVLPGRE
HHHHHHEEECCCCCCHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHCCCCEEEEECCCC
ENIEFRLPSELTSA
CCEEEECCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12597275 [H]