Click here to switch to the map view.

The map label for this gene is folK [H]

Identifier: 75676420

GI number: 75676420

Start: 2429897

End: 2430388

Strand: Direct

Name: folK [H]

Synonym: Nwi_2235

Alternate gene names: 75676420

Gene position: 2429897-2430388 (Clockwise)

Preceding gene: 75676419

Following gene: 75676421

Centisome position: 71.42

GC content: 65.45

Gene sequence:

>492_bases
ATGGCCAGCGTCCTGATCGCGCTCGGGGGTAACGTCGGAGACGTCAGGCAGACGTTCCGCAAGGCCATCGCCAACATCCG
CGGCATGGCGCAGGCCGCGCTGCTCGCCCGTTCGTCCGACTACGCCACGCCGCCATGGGGCGAGGAGAAACAGGCTCGTT
TCGTCAACGCCTGCATCGAGATCGACACCGGCCTCGACCCGCACGCTTTGCTGTTCACGCTGCAAAAGATCGAGAAGAAA
TTCGGCCGCGACCGCGCCGCCGAACAACGCTGGGGACCGCGCACGCTGGACCTCGACCTGATCGCCTATGACGACGCCAT
TCTCGACAAGCCGGAACTGACGCTACCGCATCCGCGCCTGTTCGAGCGCGCCTTCGTTCTGGTTCCGCTTGCCGAAATCG
CGCCGGACCGCATTATCGCAGGGCAAACACCGGCCAGGGCGCTGGCCGACATATCGACCGCAGGAATCGAGCGCCTCCCC
GACCTTCCCTGA

Upstream 100 bases:

>100_bases
GGACGATCACGATCACCGTGCACAAGCCGCACGCGCCGATCCCGGCGATCTTCGACGACGTCGGCATTTCACTTACCCGC
GGCAGGGACTAGCCTTCGCG

Downstream 100 bases:

>100_bases
TCGCGCGCAAACGACCGTTTGGCTTGTCCGCCGCTGCGTGGCATTTTGAGAATCCGGATTCTGCCGGACACGAGCGAACA
ATGCCCGCTGCTCCCCATGA

Product: 7, 8-dihydro-6-hydroxymethylpterin-pyrophosphokinase

Products: NA

Alternate protein names: 6-hydroxymethyl-7,8-dihydropterin pyrophosphokinase; PPPK; 7,8-dihydro-6-hydroxymethylpterin-pyrophosphokinase; HPPK [H]

Number of amino acids: Translated: 163; Mature: 162

Protein sequence:

>163_residues
MASVLIALGGNVGDVRQTFRKAIANIRGMAQAALLARSSDYATPPWGEEKQARFVNACIEIDTGLDPHALLFTLQKIEKK
FGRDRAAEQRWGPRTLDLDLIAYDDAILDKPELTLPHPRLFERAFVLVPLAEIAPDRIIAGQTPARALADISTAGIERLP
DLP

Sequences:

>Translated_163_residues
MASVLIALGGNVGDVRQTFRKAIANIRGMAQAALLARSSDYATPPWGEEKQARFVNACIEIDTGLDPHALLFTLQKIEKK
FGRDRAAEQRWGPRTLDLDLIAYDDAILDKPELTLPHPRLFERAFVLVPLAEIAPDRIIAGQTPARALADISTAGIERLP
DLP
>Mature_162_residues
ASVLIALGGNVGDVRQTFRKAIANIRGMAQAALLARSSDYATPPWGEEKQARFVNACIEIDTGLDPHALLFTLQKIEKKF
GRDRAAEQRWGPRTLDLDLIAYDDAILDKPELTLPHPRLFERAFVLVPLAEIAPDRIIAGQTPARALADISTAGIERLPD
LP

Specific function: Dihydrofolate biosynthesis; first step. [C]

COG id: COG0801

COG function: function code H; 7,8-dihydro-6-hydroxymethylpterin-pyrophosphokinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HPPK family [H]

Homologues:

Organism=Escherichia coli, GI1786335, Length=139, Percent_Identity=38.1294964028777, Blast_Score=102, Evalue=2e-23,
Organism=Saccharomyces cerevisiae, GI6324073, Length=134, Percent_Identity=35.0746268656716, Blast_Score=85, Evalue=5e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000550 [H]

Pfam domain/function: PF01288 HPPK [H]

EC number: =2.7.6.3 [H]

Molecular weight: Translated: 17893; Mature: 17762

Theoretical pI: Translated: 5.81; Mature: 5.81

Prosite motif: PS00794 HPPK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASVLIALGGNVGDVRQTFRKAIANIRGMAQAALLARSSDYATPPWGEEKQARFVNACIE
CCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHH
IDTGLDPHALLFTLQKIEKKFGRDRAAEQRWGPRTLDLDLIAYDDAILDKPELTLPHPRL
HCCCCCHHHHHHHHHHHHHHHCCCHHHHHHCCCCEEEEEEEEECHHHCCCCCCCCCCHHH
FERAFVLVPLAEIAPDRIIAGQTPARALADISTAGIERLPDLP
HHHHHHHHHHHHHCCCCEECCCCCHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
ASVLIALGGNVGDVRQTFRKAIANIRGMAQAALLARSSDYATPPWGEEKQARFVNACIE
CEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHH
IDTGLDPHALLFTLQKIEKKFGRDRAAEQRWGPRTLDLDLIAYDDAILDKPELTLPHPRL
HCCCCCHHHHHHHHHHHHHHHCCCHHHHHHCCCCEEEEEEEEECHHHCCCCCCCCCCHHH
FERAFVLVPLAEIAPDRIIAGQTPARALADISTAGIERLPDLP
HHHHHHHHHHHHHCCCCEECCCCCHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9168622 [H]