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The map label for this gene is pepT [H]

Identifier: 75676084

GI number: 75676084

Start: 2074083

End: 2075336

Strand: Direct

Name: pepT [H]

Synonym: Nwi_1893

Alternate gene names: 75676084

Gene position: 2074083-2075336 (Clockwise)

Preceding gene: 75676083

Following gene: 75676086

Centisome position: 60.96

GC content: 60.69

Gene sequence:

>1254_bases
ATGACCGCTCCCATGACGTTCCATCACACGGTAACGGAGCGCTTCCTTCGCTATGTCGTCATTGATACCCAGTCCGATCC
GGCGTCTTCGAGTTATCCCTCCACCGAGAAGCAGAAGGATCTCGGGCGTCTGCTGGTGAGCGAGTTACAGGAACTCGGCA
TCGCGGATGCTCATCTCGACTCGCACGGCTACGTGTATGCGACGCTTCCGGCGAACAGCTCGAAGCAGGTTCCGGTTATC
TGCTTCTGTTCGCATATGGACACCTCGCCGGATTGTTCAGGAGCAAACGTCAAACCGCAGATCGTCCGGAACTATCGAGG
CGGCGACATCCATCTCCGCGGCGATCCGTCGCAGATCATCCGCGCGGCGGATCATCCGGCGCTGGCTGATCAGGTCGGCA
ACGATATCATCACGTCGGACGGCACCACGCTGCTCGGCGCCGACAACAAAGCCGGCGTTGCCGAAATCATGGATGCGGTT
CAGGTCCTGCTCGCCAATCCGGAGGTCAGGCATGGCACGATCAAAATCCTGTTCACGCCCGATGAGGAAATCGGACGCGG
TGTCGATAAGGTCGACCTGAAAAAACTCGGCGCGGACTTCGCCTACACGGTCGACGGCGAGACCGCGGGTTATATCGAGG
ACGAGACGTTCTCGGCCGATGCCGCGCTTATTACCATCAAGGGAGTCAGCGCCCATCCCGGCTTCGCCAAGGGAAAGATG
GAACATGCGATCAAGATCGCGTCCCGCATCGTGGAACGGCTCCCCAGGAATGCCTGCTCGCCCGAGACGACCGACGGCCG
CGAAGGCTTTCTGCACCCGACCGACATCAGCGGCACGCTCGAAAGCGCGACGCTGAGCCTCATCGTGCGCGATTTCACGG
AAACGGGCCTGACTGAGAAGAAGATGCTGCTGGAGGACATCATCCGCGACATCATGGCGGACTTCCCACACTCGACCTGT
CGGCTGGAAATTCAGCCGCAGTATCGCAACATGAAAGAGGTTGTGGATCGTCACCCCGAGGTTGTCGATAACGCGATGGA
GGCGATCCGCCGCGCCGGTCTCGAACCGGTCAAAGGCAGCGTTCGCGGCGGCACGGACGGCTCGCGACTGTCATTCATGG
GACTTCCCTGTCCCAACATCTTCGCCGGCGAACACGCCTTCCACTCGCGCACCGAGTGGGTCAGCGTGCAAGACATGGAA
GCTGCCGTGCGAACCATCGTGCACCTCGCCGCGATCTGGGAAGAACGCGCCTAG

Upstream 100 bases:

>100_bases
ATTCAACCGATAGCCCACGCAAGGCCGGGATCACTCACCTGGCGAGGTTGATTCCATTGCCGATGTTGATTCCCTGCTTT
ACGGTCCCCCTGGATTCCCG

Downstream 100 bases:

>100_bases
ATCGTTTTCGAACGAAGCATGTCCTCGGGCTTGACCCGAGGATGGAATCCGGTTCGCGTGAAGAAAACGCGTCAGGTAAA
TATCATAGAGCATCGCTTCT

Product: peptidase T

Products: NA

Alternate protein names: Aminotripeptidase; Tripeptidase; Tripeptide aminopeptidase [H]

Number of amino acids: Translated: 417; Mature: 416

Protein sequence:

>417_residues
MTAPMTFHHTVTERFLRYVVIDTQSDPASSSYPSTEKQKDLGRLLVSELQELGIADAHLDSHGYVYATLPANSSKQVPVI
CFCSHMDTSPDCSGANVKPQIVRNYRGGDIHLRGDPSQIIRAADHPALADQVGNDIITSDGTTLLGADNKAGVAEIMDAV
QVLLANPEVRHGTIKILFTPDEEIGRGVDKVDLKKLGADFAYTVDGETAGYIEDETFSADAALITIKGVSAHPGFAKGKM
EHAIKIASRIVERLPRNACSPETTDGREGFLHPTDISGTLESATLSLIVRDFTETGLTEKKMLLEDIIRDIMADFPHSTC
RLEIQPQYRNMKEVVDRHPEVVDNAMEAIRRAGLEPVKGSVRGGTDGSRLSFMGLPCPNIFAGEHAFHSRTEWVSVQDME
AAVRTIVHLAAIWEERA

Sequences:

>Translated_417_residues
MTAPMTFHHTVTERFLRYVVIDTQSDPASSSYPSTEKQKDLGRLLVSELQELGIADAHLDSHGYVYATLPANSSKQVPVI
CFCSHMDTSPDCSGANVKPQIVRNYRGGDIHLRGDPSQIIRAADHPALADQVGNDIITSDGTTLLGADNKAGVAEIMDAV
QVLLANPEVRHGTIKILFTPDEEIGRGVDKVDLKKLGADFAYTVDGETAGYIEDETFSADAALITIKGVSAHPGFAKGKM
EHAIKIASRIVERLPRNACSPETTDGREGFLHPTDISGTLESATLSLIVRDFTETGLTEKKMLLEDIIRDIMADFPHSTC
RLEIQPQYRNMKEVVDRHPEVVDNAMEAIRRAGLEPVKGSVRGGTDGSRLSFMGLPCPNIFAGEHAFHSRTEWVSVQDME
AAVRTIVHLAAIWEERA
>Mature_416_residues
TAPMTFHHTVTERFLRYVVIDTQSDPASSSYPSTEKQKDLGRLLVSELQELGIADAHLDSHGYVYATLPANSSKQVPVIC
FCSHMDTSPDCSGANVKPQIVRNYRGGDIHLRGDPSQIIRAADHPALADQVGNDIITSDGTTLLGADNKAGVAEIMDAVQ
VLLANPEVRHGTIKILFTPDEEIGRGVDKVDLKKLGADFAYTVDGETAGYIEDETFSADAALITIKGVSAHPGFAKGKME
HAIKIASRIVERLPRNACSPETTDGREGFLHPTDISGTLESATLSLIVRDFTETGLTEKKMLLEDIIRDIMADFPHSTCR
LEIQPQYRNMKEVVDRHPEVVDNAMEAIRRAGLEPVKGSVRGGTDGSRLSFMGLPCPNIFAGEHAFHSRTEWVSVQDMEA
AVRTIVHLAAIWEERA

Specific function: Cleaves the N-terminal amino acid of tripeptides [H]

COG id: COG2195

COG function: function code E; Di- and tripeptidases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M20B family [H]

Homologues:

Organism=Escherichia coli, GI1787372, Length=408, Percent_Identity=45.0980392156863, Blast_Score=349, Evalue=2e-97,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001261
- InterPro:   IPR002933
- InterPro:   IPR011650
- InterPro:   IPR010161 [H]

Pfam domain/function: PF07687 M20_dimer; PF01546 Peptidase_M20 [H]

EC number: =3.4.11.4 [H]

Molecular weight: Translated: 45554; Mature: 45423

Theoretical pI: Translated: 5.19; Mature: 5.19

Prosite motif: PS00759 ARGE_DAPE_CPG2_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAPMTFHHTVTERFLRYVVIDTQSDPASSSYPSTEKQKDLGRLLVSELQELGIADAHLD
CCCCCCHHHHHHHHHHEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEC
SHGYVYATLPANSSKQVPVICFCSHMDTSPDCSGANVKPQIVRNYRGGDIHLRGDPSQII
CCCEEEEEECCCCCCCCEEEEEECCCCCCCCCCCCCCCHHHHHCCCCCEEEECCCHHHHH
RAADHPALADQVGNDIITSDGTTLLGADNKAGVAEIMDAVQVLLANPEVRHGTIKILFTP
HHCCCCHHHHHHCCCEEECCCCEEEECCCCCCHHHHHHHHHHHHCCCCCCCCEEEEEECC
DEEIGRGVDKVDLKKLGADFAYTVDGETAGYIEDETFSADAALITIKGVSAHPGFAKGKM
CHHHHCCCCHHHHHHHCCCEEEEECCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCHHHH
EHAIKIASRIVERLPRNACSPETTDGREGFLHPTDISGTLESATLSLIVRDFTETGLTEK
HHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHH
KMLLEDIIRDIMADFPHSTCRLEIQPQYRNMKEVVDRHPEVVDNAMEAIRRAGLEPVKGS
HHHHHHHHHHHHHHCCCCEEEEEECHHHCCHHHHHHCCHHHHHHHHHHHHHCCCCCCCCC
VRGGTDGSRLSFMGLPCPNIFAGEHAFHSRTEWVSVQDMEAAVRTIVHLAAIWEERA
CCCCCCCCEEEEECCCCCCCCCCCHHHCCCCCEEEHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TAPMTFHHTVTERFLRYVVIDTQSDPASSSYPSTEKQKDLGRLLVSELQELGIADAHLD
CCCCCHHHHHHHHHHEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEC
SHGYVYATLPANSSKQVPVICFCSHMDTSPDCSGANVKPQIVRNYRGGDIHLRGDPSQII
CCCEEEEEECCCCCCCCEEEEEECCCCCCCCCCCCCCCHHHHHCCCCCEEEECCCHHHHH
RAADHPALADQVGNDIITSDGTTLLGADNKAGVAEIMDAVQVLLANPEVRHGTIKILFTP
HHCCCCHHHHHHCCCEEECCCCEEEECCCCCCHHHHHHHHHHHHCCCCCCCCEEEEEECC
DEEIGRGVDKVDLKKLGADFAYTVDGETAGYIEDETFSADAALITIKGVSAHPGFAKGKM
CHHHHCCCCHHHHHHHCCCEEEEECCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCHHHH
EHAIKIASRIVERLPRNACSPETTDGREGFLHPTDISGTLESATLSLIVRDFTETGLTEK
HHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHH
KMLLEDIIRDIMADFPHSTCRLEIQPQYRNMKEVVDRHPEVVDNAMEAIRRAGLEPVKGS
HHHHHHHHHHHHHHCCCCEEEEEECHHHCCHHHHHHCCHHHHHHHHHHHHHCCCCCCCCC
VRGGTDGSRLSFMGLPCPNIFAGEHAFHSRTEWVSVQDMEAAVRTIVHLAAIWEERA
CCCCCCCCEEEEECCCCCCCCCCCHHHCCCCCEEEHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11997336 [H]