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The map label for this gene is trmFO

Identifier: 75675956

GI number: 75675956

Start: 1934176

End: 1935609

Strand: Direct

Name: trmFO

Synonym: Nwi_1764

Alternate gene names: 75675956

Gene position: 1934176-1935609 (Clockwise)

Preceding gene: 75675955

Following gene: 75675957

Centisome position: 56.85

GC content: 65.2

Gene sequence:

>1434_bases
ATGACACAGTTTGAATCCTCCCGCGCCACCGTCCATATCGTCGGCGCCGGCCTGGCCGGCTCGGAAGCGGCCTGGCAGGT
CGCCGCTCAGGGCGTTCGGGTCGTGCTGCACGAGATGCGGCCCCACCGGATGACCGCGGCGCACCGGACCGGTGGCCTTG
CGGAACTGGTCTGTTCAAATTCGTTCCGGTCCGACGATGCTTCCAACAATGCGGTCGGACTCCTCCATGCGGAGATGCGC
CGGCTCGGATCGCTGGTCATGCGATGCGCCGATGCCAATCAGGTTCCTGCAGGCGGCGCGCTCGCGGTGGATCGCGACGG
CTTTTCCGCCGCGGTCACCGAAGCTCTCGACAACCATCCGCTGATCGAGATCGACCGTGCCGAGATCGACGGCCTGCCGC
CCGCGGACTGGGGCAACGTCATTGTCGCCACGGGGCCGCTGACCTCGACGCCTCTCGCGGCTGCGATCCGCGCACTGACC
GACGAGTCCGCCCTCGCCTTCTTCGATGCGATCGCACCCATCGTCCATCGCGACTCCATCGATATGTCGAAAGCCTGGTT
CCAGTCCCGCTATGACAAGGCTGGTCCTGGCGGCAGCGGCGCCGATTACATCAATTGTCCGCTGAGCGAAGCGCAGTATC
ACGCCTTTGTGGACGCGCTGCTCGAGGGCGAGAAGGTCGACTTCAAGGACTGGGAGACCGCGCCCCCCTACTTCGACGGC
TGCCTGCCGATCGAGGTCATGGCCGAACGCGGCCGGGAAACGCTGCGCTATGGTCCGATGAAGCCGGTCGGCCTCACCAA
TCCGCACGATCCCGCCACCAAGCCCTACGCCGTCGTCCAATTGCGCCAGGACAACAAGCTGGGCACGCTTTACAATATCG
TGGGCTTTCAGACCAAACTGAAGCATGGCGCGCAGACGCGAATCTTTCGCACCATTCCCGGCCTTGAAGGCGCGGAGTTT
GCCCGCCTCGGCGGCCTGCATCGCAACACATTCCTGAACTCGCCAAAGCTTCTTGACGTCCGGTTGCGGCTGCGCGCCGA
GCCACGGCTGCGCTTCGCGGGACAGATGACCGGATGCGAAGGTTACGTGGAATCCGCCGCTATCGGGCTGCTCGGGGGAC
TCTACGCGGCAGCCGATGCACGATCATGCACGCTTGAAGCCCCGCCGCAGACGACGGCGCTGGGCGCGCTGCTCGGCCAC
ATCACCGGCGGCCACATCGAGACCATCGACACGGGCCCCCGCTCGTTCCAGCCGATGAACATCAATTTCGGATTGTTTCC
TCCGCTCGCCGATCCGCCAACCAGGAAGCCTGACGGCACGCGCCTGCGCGGCAATGAAAAGACGATCGCAAAGAAACAGG
CGATCTGCGCGCGCGCGCTGTCGGATCTCGATCGCTGGATCGCGGATGCGCTTCGTCCGGCAGCCGCGGCATAG

Upstream 100 bases:

>100_bases
ACCATAGCCCCAATGCGGACGCGAATACCGTCAGGGTTGAAAGCGCCGGACCCGCGTTAACGCGTTCCTTTTGGCTGAGA
AACGCGCTACCCCTTCGTCC

Downstream 100 bases:

>100_bases
GTTCAACACGGCACCTCCAGGATTATCATGCCCCTTCCGAAGCAGGACGCAGCATTCCTGTCCACGAACTGGACCGAAGG
AGTCCTACTGAAACGCGACG

Product: tRNA (uracil-5-)-methyltransferase Gid

Products: NA

Alternate protein names: Folate-dependent tRNA (uracil-5-)-methyltransferase; Folate-dependent tRNA(M-5-U54)-methyltransferase

Number of amino acids: Translated: 477; Mature: 476

Protein sequence:

>477_residues
MTQFESSRATVHIVGAGLAGSEAAWQVAAQGVRVVLHEMRPHRMTAAHRTGGLAELVCSNSFRSDDASNNAVGLLHAEMR
RLGSLVMRCADANQVPAGGALAVDRDGFSAAVTEALDNHPLIEIDRAEIDGLPPADWGNVIVATGPLTSTPLAAAIRALT
DESALAFFDAIAPIVHRDSIDMSKAWFQSRYDKAGPGGSGADYINCPLSEAQYHAFVDALLEGEKVDFKDWETAPPYFDG
CLPIEVMAERGRETLRYGPMKPVGLTNPHDPATKPYAVVQLRQDNKLGTLYNIVGFQTKLKHGAQTRIFRTIPGLEGAEF
ARLGGLHRNTFLNSPKLLDVRLRLRAEPRLRFAGQMTGCEGYVESAAIGLLGGLYAAADARSCTLEAPPQTTALGALLGH
ITGGHIETIDTGPRSFQPMNINFGLFPPLADPPTRKPDGTRLRGNEKTIAKKQAICARALSDLDRWIADALRPAAAA

Sequences:

>Translated_477_residues
MTQFESSRATVHIVGAGLAGSEAAWQVAAQGVRVVLHEMRPHRMTAAHRTGGLAELVCSNSFRSDDASNNAVGLLHAEMR
RLGSLVMRCADANQVPAGGALAVDRDGFSAAVTEALDNHPLIEIDRAEIDGLPPADWGNVIVATGPLTSTPLAAAIRALT
DESALAFFDAIAPIVHRDSIDMSKAWFQSRYDKAGPGGSGADYINCPLSEAQYHAFVDALLEGEKVDFKDWETAPPYFDG
CLPIEVMAERGRETLRYGPMKPVGLTNPHDPATKPYAVVQLRQDNKLGTLYNIVGFQTKLKHGAQTRIFRTIPGLEGAEF
ARLGGLHRNTFLNSPKLLDVRLRLRAEPRLRFAGQMTGCEGYVESAAIGLLGGLYAAADARSCTLEAPPQTTALGALLGH
ITGGHIETIDTGPRSFQPMNINFGLFPPLADPPTRKPDGTRLRGNEKTIAKKQAICARALSDLDRWIADALRPAAAA
>Mature_476_residues
TQFESSRATVHIVGAGLAGSEAAWQVAAQGVRVVLHEMRPHRMTAAHRTGGLAELVCSNSFRSDDASNNAVGLLHAEMRR
LGSLVMRCADANQVPAGGALAVDRDGFSAAVTEALDNHPLIEIDRAEIDGLPPADWGNVIVATGPLTSTPLAAAIRALTD
ESALAFFDAIAPIVHRDSIDMSKAWFQSRYDKAGPGGSGADYINCPLSEAQYHAFVDALLEGEKVDFKDWETAPPYFDGC
LPIEVMAERGRETLRYGPMKPVGLTNPHDPATKPYAVVQLRQDNKLGTLYNIVGFQTKLKHGAQTRIFRTIPGLEGAEFA
RLGGLHRNTFLNSPKLLDVRLRLRAEPRLRFAGQMTGCEGYVESAAIGLLGGLYAAADARSCTLEAPPQTTALGALLGHI
TGGHIETIDTGPRSFQPMNINFGLFPPLADPPTRKPDGTRLRGNEKTIAKKQAICARALSDLDRWIADALRPAAAA

Specific function: Catalyzes the folate-dependent formation of 5-methyl- uridine at position 54 (M-5-U54) in all tRNAs

COG id: COG1206

COG function: function code J; NAD(FAD)-utilizing enzyme possibly involved in translation

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MnmG family. TrmFO subfamily

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRMFO_NITWN (Q3SRR6)

Other databases:

- EMBL:   CP000115
- RefSeq:   YP_318377.1
- ProteinModelPortal:   Q3SRR6
- SMR:   Q3SRR6
- STRING:   Q3SRR6
- GeneID:   3675644
- GenomeReviews:   CP000115_GR
- KEGG:   nwi:Nwi_1764
- NMPDR:   fig|323098.3.peg.1598
- eggNOG:   COG1206
- HOGENOM:   HBG285041
- OMA:   FEGCLPI
- ProtClustDB:   PRK05335
- BioCyc:   NWIN323098:NWI_1764-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01037
- InterPro:   IPR004417
- InterPro:   IPR002218
- InterPro:   IPR016040
- Gene3D:   G3DSA:3.40.50.720
- TIGRFAMs:   TIGR00137

Pfam domain/function: PF01134 GIDA

EC number: =2.1.1.74

Molecular weight: Translated: 51183; Mature: 51052

Theoretical pI: Translated: 6.84; Mature: 6.84

Prosite motif: PS01280 GIDA_1; PS01281 GIDA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQFESSRATVHIVGAGLAGSEAAWQVAAQGVRVVLHEMRPHRMTAAHRTGGLAELVCSN
CCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCHHHHHHCC
SFRSDDASNNAVGLLHAEMRRLGSLVMRCADANQVPAGGALAVDRDGFSAAVTEALDNHP
CCCCCCCCCCEEEHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCHHHHHHHHCCCC
LIEIDRAEIDGLPPADWGNVIVATGPLTSTPLAAAIRALTDESALAFFDAIAPIVHRDSI
EEEEEHHHCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHCCCC
DMSKAWFQSRYDKAGPGGSGADYINCPLSEAQYHAFVDALLEGEKVDFKDWETAPPYFDG
CHHHHHHHHHCCCCCCCCCCCCEEECCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCC
CLPIEVMAERGRETLRYGPMKPVGLTNPHDPATKPYAVVQLRQDNKLGTLYNIVGFQTKL
CCHHHHHHHCCHHHHHCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHH
KHGAQTRIFRTIPGLEGAEFARLGGLHRNTFLNSPKLLDVRLRLRAEPRLRFAGQMTGCE
HCCCHHEEEECCCCCCCCHHHHHCCCHHHHHCCCCCEEEEEEEECCCCCEEECCCCCCCH
GYVESAAIGLLGGLYAAADARSCTLEAPPQTTALGALLGHITGGHIETIDTGPRSFQPMN
HHHHHHHHHHHHHHHHHCCCCCEEECCCCCHHHHHHHHHHCCCCEEEEECCCCCCCCCEE
INFGLFPPLADPPTRKPDGTRLRGNEKTIAKKQAICARALSDLDRWIADALRPAAAA
CCCCCCCCCCCCCCCCCCCCEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TQFESSRATVHIVGAGLAGSEAAWQVAAQGVRVVLHEMRPHRMTAAHRTGGLAELVCSN
CCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCHHHHHHCC
SFRSDDASNNAVGLLHAEMRRLGSLVMRCADANQVPAGGALAVDRDGFSAAVTEALDNHP
CCCCCCCCCCEEEHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCHHHHHHHHCCCC
LIEIDRAEIDGLPPADWGNVIVATGPLTSTPLAAAIRALTDESALAFFDAIAPIVHRDSI
EEEEEHHHCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHCCCC
DMSKAWFQSRYDKAGPGGSGADYINCPLSEAQYHAFVDALLEGEKVDFKDWETAPPYFDG
CHHHHHHHHHCCCCCCCCCCCCEEECCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCC
CLPIEVMAERGRETLRYGPMKPVGLTNPHDPATKPYAVVQLRQDNKLGTLYNIVGFQTKL
CCHHHHHHHCCHHHHHCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHH
KHGAQTRIFRTIPGLEGAEFARLGGLHRNTFLNSPKLLDVRLRLRAEPRLRFAGQMTGCE
HCCCHHEEEECCCCCCCCHHHHHCCCHHHHHCCCCCEEEEEEEECCCCCEEECCCCCCCH
GYVESAAIGLLGGLYAAADARSCTLEAPPQTTALGALLGHITGGHIETIDTGPRSFQPMN
HHHHHHHHHHHHHHHHHCCCCCEEECCCCCHHHHHHHHHHCCCCEEEEECCCCCCCCCEE
INFGLFPPLADPPTRKPDGTRLRGNEKTIAKKQAICARALSDLDRWIADALRPAAAA
CCCCCCCCCCCCCCCCCCCCEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA