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The map label for this gene is adhI [H]

Identifier: 75675931

GI number: 75675931

Start: 1900674

End: 1901801

Strand: Direct

Name: adhI [H]

Synonym: Nwi_1739

Alternate gene names: 75675931

Gene position: 1900674-1901801 (Clockwise)

Preceding gene: 75675930

Following gene: 75675935

Centisome position: 55.87

GC content: 64.18

Gene sequence:

>1128_bases
ATGCACGTCCGCGCCGCAGTCGCCCTTGCCGCCGGCAAGCCGCTTGAAGTCACGACCGTGGAACTCGACGGCCCACGCGA
TGGTGAGGTCATGGTCGAGATCAAGGCGGCCGGCGTCTGCCACACCGACGAGTTCACCTTGTCCGGCGCGGATCCGGAAG
GGTTGTTTCCGGCCATACTCGGCCATGAGGGCGCCGGCGTGGTGGTCGATGTCGGCAAGGGCGTGACCTCGGTGAAGAAA
GGCGATCACGTTATTCCGCTCTACACGCCCGAATGCCGGCAATGCCCGTCCTGCCTGTCGCGCAAGACCAATCTGTGCAC
GGCCATTCGCGCGACCCAAGGCCAGGGGTTAATGCCGGACGGCACCTCGCGCTTCTCCTTCGACGGCAAGCCGCTCCATC
ACTACATGGGCACCTCGACCTTCGCCAACTACACGGTGCTGCCGGAGATCGCGGTCGCGAAGATCCGCGAAGACGCTCCG
TTCGACAAGGTCTGCTACATCGGCTGCGGCGTCACTACCGGCATCGGCGCGGTGATCAACACCGCGAAGGTCGAACCCGG
CGCCAAGGCGATCGTGTTCGGCCTCGGCGGCATCGGGCTGAACGTCCTGCAAGGGTTGAGGCTCGCCGGCGCCGACATGA
TCATCGGCGTGGACATCAACGACGATCGCAAGGCGTGGGGCGAGCGCTTCGGCATGACGCACTTCGTCAATCCCACGGAT
GTCGGGAAGGAACTCGTCCCTTACCTTGTCGATATGACGAAGTCCGGCGCTGATCAGATCGGCGGCGCGGATTACACGTT
CGACTGCACGGGCAATGTGAAGGTGATGCGCCAGGCGCTGGAATCCTGTCACCGTGGCTGGGGACAGTCGATCGTGATCG
GCGTCGCCCCCGCCGGCGCGGAGATCGCGACGCGGCCGTTCCAACTCGTCACAGGCCGCGTATGGAAAGGCACGGCGTTC
GGCGGCGCGCGCGGCCGCACCGATGTGCCGAAGATCGTAGACTGGTACATGCAGGGCAAGATCCAGATCGATCCGATGAT
CACGCACGTCATGCCGCTCGACGACATCAACAGAGCGTTTGACCTGATGCGCAAAGGCGAATCGATCCGCGGCGTCGTCA
CGTTCTGA

Upstream 100 bases:

>100_bases
GCGGCGGCCGTCGTTGCGGCTTCGGAGGCACGACCGTATCCTGGGCGGCAACGCCCATTCCGTCGATGCTCTTTCAATTC
CTGCCGGAGAAGAAGCCGCC

Downstream 100 bases:

>100_bases
AAGAGACTTTCCGTTCCGCCGGAATCAGAAGCAAGGCTCTCTGATCTTGTTTTAAAGCGTTTTCCTCACGCGAACAGTTC
ATCCATCCTTAGGGCTCAAG

Product: zinc-containing alcohol dehydrogenase superfamily protein

Products: NA

Alternate protein names: Alcohol dehydrogenase class-III; Glutathione-dependent formaldehyde dehydrogenase; FALDH; FDH; GSH-FDH; S-(hydroxymethyl)glutathione dehydrogenase [H]

Number of amino acids: Translated: 375; Mature: 375

Protein sequence:

>375_residues
MHVRAAVALAAGKPLEVTTVELDGPRDGEVMVEIKAAGVCHTDEFTLSGADPEGLFPAILGHEGAGVVVDVGKGVTSVKK
GDHVIPLYTPECRQCPSCLSRKTNLCTAIRATQGQGLMPDGTSRFSFDGKPLHHYMGTSTFANYTVLPEIAVAKIREDAP
FDKVCYIGCGVTTGIGAVINTAKVEPGAKAIVFGLGGIGLNVLQGLRLAGADMIIGVDINDDRKAWGERFGMTHFVNPTD
VGKELVPYLVDMTKSGADQIGGADYTFDCTGNVKVMRQALESCHRGWGQSIVIGVAPAGAEIATRPFQLVTGRVWKGTAF
GGARGRTDVPKIVDWYMQGKIQIDPMITHVMPLDDINRAFDLMRKGESIRGVVTF

Sequences:

>Translated_375_residues
MHVRAAVALAAGKPLEVTTVELDGPRDGEVMVEIKAAGVCHTDEFTLSGADPEGLFPAILGHEGAGVVVDVGKGVTSVKK
GDHVIPLYTPECRQCPSCLSRKTNLCTAIRATQGQGLMPDGTSRFSFDGKPLHHYMGTSTFANYTVLPEIAVAKIREDAP
FDKVCYIGCGVTTGIGAVINTAKVEPGAKAIVFGLGGIGLNVLQGLRLAGADMIIGVDINDDRKAWGERFGMTHFVNPTD
VGKELVPYLVDMTKSGADQIGGADYTFDCTGNVKVMRQALESCHRGWGQSIVIGVAPAGAEIATRPFQLVTGRVWKGTAF
GGARGRTDVPKIVDWYMQGKIQIDPMITHVMPLDDINRAFDLMRKGESIRGVVTF
>Mature_375_residues
MHVRAAVALAAGKPLEVTTVELDGPRDGEVMVEIKAAGVCHTDEFTLSGADPEGLFPAILGHEGAGVVVDVGKGVTSVKK
GDHVIPLYTPECRQCPSCLSRKTNLCTAIRATQGQGLMPDGTSRFSFDGKPLHHYMGTSTFANYTVLPEIAVAKIREDAP
FDKVCYIGCGVTTGIGAVINTAKVEPGAKAIVFGLGGIGLNVLQGLRLAGADMIIGVDINDDRKAWGERFGMTHFVNPTD
VGKELVPYLVDMTKSGADQIGGADYTFDCTGNVKVMRQALESCHRGWGQSIVIGVAPAGAEIATRPFQLVTGRVWKGTAF
GGARGRTDVPKIVDWYMQGKIQIDPMITHVMPLDDINRAFDLMRKGESIRGVVTF

Specific function: Oxidizes long-chain aliphatic alcohols, long-chain hydroxylated fatty acids and S-hydroxymethylglutathione (hmGSH) in increasing order of preference. Shows little or no activity with short-chain aliphatic alcohols [H]

COG id: COG1062

COG function: function code C; Zn-dependent alcohol dehydrogenases, class III

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the zinc-containing alcohol dehydrogenase family. Class-III subfamily [H]

Homologues:

Organism=Homo sapiens, GI71565154, Length=373, Percent_Identity=58.9812332439678, Blast_Score=441, Evalue=1e-124,
Organism=Homo sapiens, GI34577061, Length=375, Percent_Identity=44.5333333333333, Blast_Score=339, Evalue=3e-93,
Organism=Homo sapiens, GI4501933, Length=375, Percent_Identity=44.2666666666667, Blast_Score=335, Evalue=5e-92,
Organism=Homo sapiens, GI262073058, Length=376, Percent_Identity=44.1489361702128, Blast_Score=335, Evalue=6e-92,
Organism=Homo sapiens, GI71743840, Length=376, Percent_Identity=44.1489361702128, Blast_Score=334, Evalue=8e-92,
Organism=Homo sapiens, GI71565152, Length=380, Percent_Identity=44.7368421052632, Blast_Score=334, Evalue=9e-92,
Organism=Homo sapiens, GI156523966, Length=373, Percent_Identity=43.4316353887399, Blast_Score=329, Evalue=2e-90,
Organism=Homo sapiens, GI4501929, Length=375, Percent_Identity=43.4666666666667, Blast_Score=327, Evalue=1e-89,
Organism=Homo sapiens, GI4501939, Length=367, Percent_Identity=43.5967302452316, Blast_Score=324, Evalue=8e-89,
Organism=Escherichia coli, GI1786552, Length=375, Percent_Identity=60, Blast_Score=441, Evalue=1e-125,
Organism=Escherichia coli, GI1788073, Length=370, Percent_Identity=26.2162162162162, Blast_Score=98, Evalue=7e-22,
Organism=Escherichia coli, GI226510992, Length=361, Percent_Identity=26.3157894736842, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI87082125, Length=389, Percent_Identity=24.1645244215938, Blast_Score=89, Evalue=4e-19,
Organism=Escherichia coli, GI1790045, Length=383, Percent_Identity=24.2819843342037, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI1787863, Length=354, Percent_Identity=23.4463276836158, Blast_Score=82, Evalue=8e-17,
Organism=Escherichia coli, GI87081918, Length=361, Percent_Identity=26.8698060941828, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1788075, Length=364, Percent_Identity=25.5494505494505, Blast_Score=75, Evalue=6e-15,
Organism=Escherichia coli, GI1786825, Length=373, Percent_Identity=23.3243967828418, Blast_Score=73, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI25146526, Length=379, Percent_Identity=52.2427440633245, Blast_Score=412, Evalue=1e-115,
Organism=Caenorhabditis elegans, GI71997431, Length=381, Percent_Identity=51.9685039370079, Blast_Score=407, Evalue=1e-114,
Organism=Caenorhabditis elegans, GI17565904, Length=346, Percent_Identity=54.0462427745665, Blast_Score=389, Evalue=1e-108,
Organism=Caenorhabditis elegans, GI17562584, Length=374, Percent_Identity=25.9358288770053, Blast_Score=82, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI17562582, Length=384, Percent_Identity=25.5208333333333, Blast_Score=77, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17562876, Length=361, Percent_Identity=22.9916897506925, Blast_Score=71, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI71988145, Length=377, Percent_Identity=24.1379310344828, Blast_Score=68, Evalue=9e-12,
Organism=Saccharomyces cerevisiae, GI6320033, Length=373, Percent_Identity=53.6193029490617, Blast_Score=416, Evalue=1e-117,
Organism=Saccharomyces cerevisiae, GI6319621, Length=373, Percent_Identity=28.4182305630027, Blast_Score=92, Evalue=1e-19,
Organism=Saccharomyces cerevisiae, GI6323099, Length=373, Percent_Identity=26.8096514745308, Blast_Score=90, Evalue=7e-19,
Organism=Saccharomyces cerevisiae, GI6322619, Length=359, Percent_Identity=27.2980501392758, Blast_Score=86, Evalue=1e-17,
Organism=Saccharomyces cerevisiae, GI6319955, Length=359, Percent_Identity=27.2980501392758, Blast_Score=85, Evalue=2e-17,
Organism=Saccharomyces cerevisiae, GI6323729, Length=359, Percent_Identity=25.0696378830084, Blast_Score=80, Evalue=4e-16,
Organism=Saccharomyces cerevisiae, GI6323961, Length=371, Percent_Identity=25.0673854447439, Blast_Score=76, Evalue=7e-15,
Organism=Saccharomyces cerevisiae, GI6324486, Length=361, Percent_Identity=24.6537396121884, Blast_Score=74, Evalue=4e-14,
Organism=Saccharomyces cerevisiae, GI6319257, Length=357, Percent_Identity=24.6498599439776, Blast_Score=73, Evalue=9e-14,
Organism=Saccharomyces cerevisiae, GI6319258, Length=271, Percent_Identity=26.1992619926199, Blast_Score=71, Evalue=4e-13,
Organism=Drosophila melanogaster, GI17737895, Length=374, Percent_Identity=57.4866310160428, Blast_Score=442, Evalue=1e-124,
Organism=Drosophila melanogaster, GI17137530, Length=344, Percent_Identity=27.3255813953488, Blast_Score=83, Evalue=4e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014183
- InterPro:   IPR013149
- InterPro:   IPR013154
- InterPro:   IPR002085
- InterPro:   IPR002328
- InterPro:   IPR011032
- InterPro:   IPR016040 [H]

Pfam domain/function: PF08240 ADH_N; PF00107 ADH_zinc_N [H]

EC number: =1.1.1.1; =1.1.1.284 [H]

Molecular weight: Translated: 39946; Mature: 39946

Theoretical pI: Translated: 6.78; Mature: 6.78

Prosite motif: PS00059 ADH_ZINC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHVRAAVALAAGKPLEVTTVELDGPRDGEVMVEIKAAGVCHTDEFTLSGADPEGLFPAIL
CCCCEEEEEECCCCEEEEEEEECCCCCCCEEEEEEECCEEECCCEEECCCCCCCCCHHHH
GHEGAGVVVDVGKGVTSVKKGDHVIPLYTPECRQCPSCLSRKTNLCTAIRATQGQGLMPD
CCCCCEEEEECCCCCCHHCCCCEEEEEECCHHHHHHHHHCCCCCCEEEEEECCCCCCCCC
GTSRFSFDGKPLHHYMGTSTFANYTVLPEIAVAKIREDAPFDKVCYIGCGVTTGIGAVIN
CCCEECCCCCCHHHHCCCCCCCCEEECCHHHHHHHHCCCCCCCEEEEECCHHHHHHHHEE
TAKVEPGAKAIVFGLGGIGLNVLQGLRLAGADMIIGVDINDDRKAWGERFGMTHFVNPTD
EEECCCCCEEEEEEECCCHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHCCCEEECCHHH
VGKELVPYLVDMTKSGADQIGGADYTFDCTGNVKVMRQALESCHRGWGQSIVIGVAPAGA
HHHHHHHHHHHHHCCCHHHCCCCCEEEECCCCHHHHHHHHHHHHCCCCCEEEEEECCCCC
EIATRPFQLVTGRVWKGTAFGGARGRTDVPKIVDWYMQGKIQIDPMITHVMPLDDINRAF
CHHCCCEEHHCCCEEECCCCCCCCCCCCCHHHHHHHHCCEEEECCCEEEECCHHHHHHHH
DLMRKGESIRGVVTF
HHHHCCCCCEEEEEC
>Mature Secondary Structure
MHVRAAVALAAGKPLEVTTVELDGPRDGEVMVEIKAAGVCHTDEFTLSGADPEGLFPAIL
CCCCEEEEEECCCCEEEEEEEECCCCCCCEEEEEEECCEEECCCEEECCCCCCCCCHHHH
GHEGAGVVVDVGKGVTSVKKGDHVIPLYTPECRQCPSCLSRKTNLCTAIRATQGQGLMPD
CCCCCEEEEECCCCCCHHCCCCEEEEEECCHHHHHHHHHCCCCCCEEEEEECCCCCCCCC
GTSRFSFDGKPLHHYMGTSTFANYTVLPEIAVAKIREDAPFDKVCYIGCGVTTGIGAVIN
CCCEECCCCCCHHHHCCCCCCCCEEECCHHHHHHHHCCCCCCCEEEEECCHHHHHHHHEE
TAKVEPGAKAIVFGLGGIGLNVLQGLRLAGADMIIGVDINDDRKAWGERFGMTHFVNPTD
EEECCCCCEEEEEEECCCHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHCCCEEECCHHH
VGKELVPYLVDMTKSGADQIGGADYTFDCTGNVKVMRQALESCHRGWGQSIVIGVAPAGA
HHHHHHHHHHHHHCCCHHHCCCCCEEEECCCCHHHHHHHHHHHHCCCCCEEEEEECCCCC
EIATRPFQLVTGRVWKGTAFGGARGRTDVPKIVDWYMQGKIQIDPMITHVMPLDDINRAF
CHHCCCEEHHCCCEEECCCCCCCCCCCCCHHHHHHHHCCEEEECCCEEEECCHHHHHHHH
DLMRKGESIRGVVTF
HHHHCCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8631716 [H]