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The map label for this gene is cysM [H]

Identifier: 75675717

GI number: 75675717

Start: 1664324

End: 1665382

Strand: Direct

Name: cysM [H]

Synonym: Nwi_1525

Alternate gene names: 75675717

Gene position: 1664324-1665382 (Clockwise)

Preceding gene: 75675710

Following gene: 75675722

Centisome position: 48.92

GC content: 59.96

Gene sequence:

>1059_bases
ATGAGAATTTTGCATCTCATGAGAATCGATAACGACGTCGTAGAAGCGATCGGCAATACACCTCTTATCAAGTTGAAGCG
CGCCTCCGAAGCAACAGGATGCACCATTCTCGGCAAGGCCGAGTTCATGAATCCGGGTCAGTCCGTCAAGGATCGCGCCG
GTAAGCAGATGATCCTGGAAGCCGAAAAGCGCGGCGACCTCAAGCCCGGCGGCCTCGTCGTTGAAGGCACGGCCGGCAAT
ACCGGCATCGGTCTTGCCATCGTCGCCAGCGCTAGAGGCTATCGCACGATGATCGTCATTCCGGAAACACAAAGCCGTGA
AAAGAAAGACATGCTTCGGCTATGCGGCGCCGAGCTGGTCGAGGTTCCGGCGCTCCCCTACAGCAACCCGAACAATTATC
AGCACATCGCAAGGCGTCTCGCTGACGAATTGCGCGAATCCGAACCCAACGGTGTGCTGTTCGCCGACCAATGGAATAAC
CTCGACAATCCGAAGGCCCATTACGTTTCCACGGGTCCGGAAATCTGGGAGCAGACGGATGGAAAGGTCGATGGCTTCAT
CTGTTCGATCGGCACCGGCGGCACGCTCGCCGGAGTGAGCGCTTTCCTGCGGGAGAAAAATAAAGATATCGTCATCGGCG
TCGCGGACCCGCGCGGCGCGGCCATGTATGAACTGTTCAAGAACGGCGTGGCCAAGCCGACCGCGGGAGGCTCGATCACC
GAGGGGATCGGGCTCGGACGCGTGACGCCCGTGATTGCCGACGCCAGGGTTGACGAAGCCTATCTCGTCCCGGACGAGGA
GGCTGTGCCCGTGATCTACGATCTGCTCGAACACGAGGGGCTTTGCCTTGGCGGCTCGACCGGCGTCAACGTCGCCGGCG
CGATTCGTCTCGCGCGCCAGCTGGGGCCCGGCCATACCGTCGTGACGATCCTGTGCGACTCGGGCAACCGCTATCAGTCG
AAACTTTTCAACCCGGACTTCATGCGCTCCAAGAACCTTCCCGTGCCGCAATGGCTCGAGAGGCGGAGCACGGTGAAGGT
TCCGCTCGCAGAAGTTTAA

Upstream 100 bases:

>100_bases
AGTCGCCGGCCCGCGGCTTCGCTTTATGAATGCGATTCTGCGCACCCTTGTACCTGCCTCTCCCCTCAACTAGGGTTACC
TGCAATCCTCCAACAGCCCG

Downstream 100 bases:

>100_bases
GGCCGTTCCGGTCTCACCGCAGAATCTGGCTGAGGAAGAGCTGGCTGCGCGGGTGTCGCGGATTGGCGAAGAAGCTGGCC
GGGGTATCGGACTCGATGAT

Product: cysteine synthase A

Products: NA

Alternate protein names: CSase B; O-acetylserine (thiol)-lyase B; OAS-TL B; O-acetylserine sulfhydrylase B [H]

Number of amino acids: Translated: 352; Mature: 352

Protein sequence:

>352_residues
MRILHLMRIDNDVVEAIGNTPLIKLKRASEATGCTILGKAEFMNPGQSVKDRAGKQMILEAEKRGDLKPGGLVVEGTAGN
TGIGLAIVASARGYRTMIVIPETQSREKKDMLRLCGAELVEVPALPYSNPNNYQHIARRLADELRESEPNGVLFADQWNN
LDNPKAHYVSTGPEIWEQTDGKVDGFICSIGTGGTLAGVSAFLREKNKDIVIGVADPRGAAMYELFKNGVAKPTAGGSIT
EGIGLGRVTPVIADARVDEAYLVPDEEAVPVIYDLLEHEGLCLGGSTGVNVAGAIRLARQLGPGHTVVTILCDSGNRYQS
KLFNPDFMRSKNLPVPQWLERRSTVKVPLAEV

Sequences:

>Translated_352_residues
MRILHLMRIDNDVVEAIGNTPLIKLKRASEATGCTILGKAEFMNPGQSVKDRAGKQMILEAEKRGDLKPGGLVVEGTAGN
TGIGLAIVASARGYRTMIVIPETQSREKKDMLRLCGAELVEVPALPYSNPNNYQHIARRLADELRESEPNGVLFADQWNN
LDNPKAHYVSTGPEIWEQTDGKVDGFICSIGTGGTLAGVSAFLREKNKDIVIGVADPRGAAMYELFKNGVAKPTAGGSIT
EGIGLGRVTPVIADARVDEAYLVPDEEAVPVIYDLLEHEGLCLGGSTGVNVAGAIRLARQLGPGHTVVTILCDSGNRYQS
KLFNPDFMRSKNLPVPQWLERRSTVKVPLAEV
>Mature_352_residues
MRILHLMRIDNDVVEAIGNTPLIKLKRASEATGCTILGKAEFMNPGQSVKDRAGKQMILEAEKRGDLKPGGLVVEGTAGN
TGIGLAIVASARGYRTMIVIPETQSREKKDMLRLCGAELVEVPALPYSNPNNYQHIARRLADELRESEPNGVLFADQWNN
LDNPKAHYVSTGPEIWEQTDGKVDGFICSIGTGGTLAGVSAFLREKNKDIVIGVADPRGAAMYELFKNGVAKPTAGGSIT
EGIGLGRVTPVIADARVDEAYLVPDEEAVPVIYDLLEHEGLCLGGSTGVNVAGAIRLARQLGPGHTVVTILCDSGNRYQS
KLFNPDFMRSKNLPVPQWLERRSTVKVPLAEV

Specific function: Two cysteine synthase enzymes are found. Both catalyze the same reaction. Cysteine synthase B can also use thiosulfate in place of sulfide to give cysteine thiosulfonate as a product [H]

COG id: COG0031

COG function: function code E; Cysteine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the cysteine synthase/cystathionine beta- synthase family [H]

Homologues:

Organism=Homo sapiens, GI295821202, Length=329, Percent_Identity=39.8176291793313, Blast_Score=221, Evalue=7e-58,
Organism=Homo sapiens, GI295821200, Length=329, Percent_Identity=39.8176291793313, Blast_Score=221, Evalue=7e-58,
Organism=Homo sapiens, GI4557415, Length=329, Percent_Identity=39.8176291793313, Blast_Score=221, Evalue=7e-58,
Organism=Escherichia coli, GI2367138, Length=308, Percent_Identity=40.2597402597403, Blast_Score=204, Evalue=7e-54,
Organism=Escherichia coli, GI1788754, Length=337, Percent_Identity=39.1691394658754, Blast_Score=172, Evalue=2e-44,
Organism=Caenorhabditis elegans, GI17535051, Length=308, Percent_Identity=37.6623376623377, Blast_Score=182, Evalue=3e-46,
Organism=Caenorhabditis elegans, GI17562970, Length=320, Percent_Identity=36.25, Blast_Score=177, Evalue=8e-45,
Organism=Caenorhabditis elegans, GI32566674, Length=310, Percent_Identity=37.0967741935484, Blast_Score=176, Evalue=1e-44,
Organism=Caenorhabditis elegans, GI115535073, Length=325, Percent_Identity=36.3076923076923, Blast_Score=172, Evalue=2e-43,
Organism=Caenorhabditis elegans, GI17534315, Length=335, Percent_Identity=34.0298507462687, Blast_Score=163, Evalue=1e-40,
Organism=Caenorhabditis elegans, GI17561720, Length=329, Percent_Identity=34.6504559270517, Blast_Score=156, Evalue=2e-38,
Organism=Caenorhabditis elegans, GI25147552, Length=332, Percent_Identity=31.9277108433735, Blast_Score=143, Evalue=1e-34,
Organism=Caenorhabditis elegans, GI32566672, Length=230, Percent_Identity=31.7391304347826, Blast_Score=102, Evalue=4e-22,
Organism=Caenorhabditis elegans, GI71996324, Length=332, Percent_Identity=26.5060240963855, Blast_Score=77, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6321449, Length=328, Percent_Identity=40.2439024390244, Blast_Score=205, Evalue=9e-54,
Organism=Saccharomyces cerevisiae, GI6321594, Length=349, Percent_Identity=34.0974212034384, Blast_Score=193, Evalue=4e-50,
Organism=Drosophila melanogaster, GI24643623, Length=340, Percent_Identity=37.6470588235294, Blast_Score=190, Evalue=1e-48,
Organism=Drosophila melanogaster, GI20129101, Length=340, Percent_Identity=37.6470588235294, Blast_Score=190, Evalue=1e-48,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001216
- InterPro:   IPR005856
- InterPro:   IPR005858
- InterPro:   IPR001926 [H]

Pfam domain/function: PF00291 PALP [H]

EC number: =2.5.1.47 [H]

Molecular weight: Translated: 37937; Mature: 37937

Theoretical pI: Translated: 6.38; Mature: 6.38

Prosite motif: PS00901 CYS_SYNTHASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRILHLMRIDNDVVEAIGNTPLIKLKRASEATGCTILGKAEFMNPGQSVKDRAGKQMILE
CCEEEEEECCHHHHHHHCCCCEEEEECCCCCCCEEEEECHHHCCCCCHHHHHCCCEEEEE
AEKRGDLKPGGLVVEGTAGNTGIGLAIVASARGYRTMIVIPETQSREKKDMLRLCGAELV
HHHCCCCCCCCEEEECCCCCCCCEEEEEEECCCCEEEEEECCCCCHHHHHHHHHHCHHHH
EVPALPYSNPNNYQHIARRLADELRESEPNGVLFADQWNNLDNPKAHYVSTGPEIWEQTD
HCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCEEECCCHHHHHHCC
GKVDGFICSIGTGGTLAGVSAFLREKNKDIVIGVADPRGAAMYELFKNGVAKPTAGGSIT
CCCCEEEEECCCCCHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHCCCCCCCCCCCCC
EGIGLGRVTPVIADARVDEAYLVPDEEAVPVIYDLLEHEGLCLGGSTGVNVAGAIRLARQ
CCCCCCCCCCEEECCCCCCEEECCCCCCHHHHHHHHHCCCEEECCCCCCCHHHHHHHHHH
LGPGHTVVTILCDSGNRYQSKLFNPDFMRSKNLPVPQWLERRSTVKVPLAEV
CCCCCEEEEEEECCCCHHHHHCCCCHHHCCCCCCCHHHHHCCCCCCCCCCCC
>Mature Secondary Structure
MRILHLMRIDNDVVEAIGNTPLIKLKRASEATGCTILGKAEFMNPGQSVKDRAGKQMILE
CCEEEEEECCHHHHHHHCCCCEEEEECCCCCCCEEEEECHHHCCCCCHHHHHCCCEEEEE
AEKRGDLKPGGLVVEGTAGNTGIGLAIVASARGYRTMIVIPETQSREKKDMLRLCGAELV
HHHCCCCCCCCEEEECCCCCCCCEEEEEEECCCCEEEEEECCCCCHHHHHHHHHHCHHHH
EVPALPYSNPNNYQHIARRLADELRESEPNGVLFADQWNNLDNPKAHYVSTGPEIWEQTD
HCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCEEECCCHHHHHHCC
GKVDGFICSIGTGGTLAGVSAFLREKNKDIVIGVADPRGAAMYELFKNGVAKPTAGGSIT
CCCCEEEEECCCCCHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHCCCCCCCCCCCCC
EGIGLGRVTPVIADARVDEAYLVPDEEAVPVIYDLLEHEGLCLGGSTGVNVAGAIRLARQ
CCCCCCCCCCEEECCCCCCEEECCCCCCHHHHHHHHHCCCEEECCCCCCCHHHHHHHHHH
LGPGHTVVTILCDSGNRYQSKLFNPDFMRSKNLPVPQWLERRSTVKVPLAEV
CCCCCEEEEEEECCCCHHHHHCCCCHHHCCCCCCCHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2188958; 9205837; 9278503 [H]