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The map label for this gene is lipA [H]

Identifier: 75675631

GI number: 75675631

Start: 1567715

End: 1568674

Strand: Direct

Name: lipA [H]

Synonym: Nwi_1439

Alternate gene names: 75675631

Gene position: 1567715-1568674 (Clockwise)

Preceding gene: 75675629

Following gene: 75675632

Centisome position: 46.08

GC content: 62.19

Gene sequence:

>960_bases
ATGGTCGTCATCCTCGATACGATTTCCGCCAATCCTGTCCGGCCGCGGCATCCGGAGAAGGCCAACCGGCCGGATGCGTT
GTCGCCGCCGAAACCCGACTGGATTCGCGTGCGCGCGCCGAACACGCGCGGCTATGCGAATACGCGCCGGATCGTCAAGG
AGAACGGACTGGTCACCGTATGCGAGGAGGCGGGCTGCCCGAACATCGGCGAGTGCTGGGACAAGAAACACGCCACCTTC
ATGATCATGGGCGACACCTGCACCCGCGCCTGCGCCTTCTGCAATGTCAAGACGGGAATGCCCGGTGCGATCGAGTCGTC
CGAGCCCGAATACGTGGCTGAAGCGACGCGCAAGCTGGGGTTGGCGCACGTCGTCGTCACATCCGTCGATCGCGACGATC
TCGATGACGGAGGCGCGGAACATTTCGCGCAGACGATACGCGCTATCCGTGAGCGTTGTCCGGCGACGACGATCGAGATC
CTGACGCCCGATTTTCTTCGCAAGGACGGCGCGCTGGAAAAGGTGGTGGCGGCGAAGCCCGATGTCTTCAACCACAATCT
CGAAACCGTTCCGTCGCGTTACCTGACTGTGCGGCCCGGCGCGCGCTATTTCCACTCGATCCGCCTGTTGCAACGGGTGA
AGGAGATCGACCCGGCGATCTTCACCAAGTCCGGCATCATGGTCGGGCTTGGCGAGGAGCGTCACGAGGTCTTGCAGGTG
ATGGACGATCTGCGGTCGGCGGATGTCGACTTTCTTACCATCGGGCAATACCTGCAGCCGACCCGCAAGCATCACGCCGT
GATACGTTACGTCACGCCGGAGGAGTTTTCGAGCTATGAGACTGTCGCTTACACCAAGGGTTTTCTGATGGTATCGGCAA
GCCCGCTGACGCGCTCGTCGCATCACGCCGGCGAGGACTTTGCAAAGTTGCAGGCCGCCCGCGCCACGTTGTCGCGGTAA

Upstream 100 bases:

>100_bases
AATCAAAAACGGGGCATACGATCTTGATCTGACGTGTTCTCTTCACGCGAACAGGTCGTTCGTTCCGCTCGAAAACGCCC
AAAAGTCTTCTGGATCATCC

Downstream 100 bases:

>100_bases
GCGCCACCGGGATGCCGTCGTTTTCAAGCAAGCGCCGCGTGCGTCACAGCGCGAGCGAGATGTTCGATCTCGTGGCGGAT
GTCGAGCGCTATCCGGACTT

Product: lipoyl synthase

Products: NA

Alternate protein names: Lip-syn; LS; Lipoate synthase; Lipoic acid synthase; Sulfur insertion protein lipA [H]

Number of amino acids: Translated: 319; Mature: 319

Protein sequence:

>319_residues
MVVILDTISANPVRPRHPEKANRPDALSPPKPDWIRVRAPNTRGYANTRRIVKENGLVTVCEEAGCPNIGECWDKKHATF
MIMGDTCTRACAFCNVKTGMPGAIESSEPEYVAEATRKLGLAHVVVTSVDRDDLDDGGAEHFAQTIRAIRERCPATTIEI
LTPDFLRKDGALEKVVAAKPDVFNHNLETVPSRYLTVRPGARYFHSIRLLQRVKEIDPAIFTKSGIMVGLGEERHEVLQV
MDDLRSADVDFLTIGQYLQPTRKHHAVIRYVTPEEFSSYETVAYTKGFLMVSASPLTRSSHHAGEDFAKLQAARATLSR

Sequences:

>Translated_319_residues
MVVILDTISANPVRPRHPEKANRPDALSPPKPDWIRVRAPNTRGYANTRRIVKENGLVTVCEEAGCPNIGECWDKKHATF
MIMGDTCTRACAFCNVKTGMPGAIESSEPEYVAEATRKLGLAHVVVTSVDRDDLDDGGAEHFAQTIRAIRERCPATTIEI
LTPDFLRKDGALEKVVAAKPDVFNHNLETVPSRYLTVRPGARYFHSIRLLQRVKEIDPAIFTKSGIMVGLGEERHEVLQV
MDDLRSADVDFLTIGQYLQPTRKHHAVIRYVTPEEFSSYETVAYTKGFLMVSASPLTRSSHHAGEDFAKLQAARATLSR
>Mature_319_residues
MVVILDTISANPVRPRHPEKANRPDALSPPKPDWIRVRAPNTRGYANTRRIVKENGLVTVCEEAGCPNIGECWDKKHATF
MIMGDTCTRACAFCNVKTGMPGAIESSEPEYVAEATRKLGLAHVVVTSVDRDDLDDGGAEHFAQTIRAIRERCPATTIEI
LTPDFLRKDGALEKVVAAKPDVFNHNLETVPSRYLTVRPGARYFHSIRLLQRVKEIDPAIFTKSGIMVGLGEERHEVLQV
MDDLRSADVDFLTIGQYLQPTRKHHAVIRYVTPEEFSSYETVAYTKGFLMVSASPLTRSSHHAGEDFAKLQAARATLSR

Specific function: Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives [H]

COG id: COG0320

COG function: function code H; Lipoate synthase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Lipoyl synthase family [H]

Homologues:

Organism=Homo sapiens, GI37577166, Length=293, Percent_Identity=47.4402730375427, Blast_Score=268, Evalue=4e-72,
Organism=Homo sapiens, GI37577164, Length=243, Percent_Identity=47.7366255144033, Blast_Score=230, Evalue=1e-60,
Organism=Escherichia coli, GI1786846, Length=275, Percent_Identity=50.9090909090909, Blast_Score=283, Evalue=8e-78,
Organism=Caenorhabditis elegans, GI32564533, Length=258, Percent_Identity=45.3488372093023, Blast_Score=238, Evalue=4e-63,
Organism=Saccharomyces cerevisiae, GI6324770, Length=297, Percent_Identity=45.7912457912458, Blast_Score=258, Evalue=7e-70,
Organism=Drosophila melanogaster, GI221513272, Length=283, Percent_Identity=45.2296819787986, Blast_Score=247, Evalue=9e-66,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR006638
- InterPro:   IPR003698
- InterPro:   IPR007197 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: =2.8.1.8 [H]

Molecular weight: Translated: 35509; Mature: 35509

Theoretical pI: Translated: 7.85; Mature: 7.85

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVVILDTISANPVRPRHPEKANRPDALSPPKPDWIRVRAPNTRGYANTRRIVKENGLVTV
CEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCHHHHHHHHCCCCEEE
CEEAGCPNIGECWDKKHATFMIMGDTCTRACAFCNVKTGMPGAIESSEPEYVAEATRKLG
ECCCCCCCHHHHHCCCCCEEEEECCHHHHHHHHEECCCCCCCCCCCCCCHHHHHHHHHHC
LAHVVVTSVDRDDLDDGGAEHFAQTIRAIRERCPATTIEILTPDFLRKDGALEKVVAAKP
HHEEEEEECCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEECHHHHHCCCCHHHHHHCCC
DVFNHNLETVPSRYLTVRPGARYFHSIRLLQRVKEIDPAIFTKSGIMVGLGEERHEVLQV
CHHCCCHHHCCCCEEEECCCHHHHHHHHHHHHHHHCCHHEEECCCEEEECCHHHHHHHHH
MDDLRSADVDFLTIGQYLQPTRKHHAVIRYVTPEEFSSYETVAYTKGFLMVSASPLTRSS
HHHHHHCCCCEEEHHHHHCHHHHCCEEEEEECHHHHCCCCEEEEECCEEEEECCCCCCCC
HHAGEDFAKLQAARATLSR
CCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure
MVVILDTISANPVRPRHPEKANRPDALSPPKPDWIRVRAPNTRGYANTRRIVKENGLVTV
CEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCHHHHHHHHCCCCEEE
CEEAGCPNIGECWDKKHATFMIMGDTCTRACAFCNVKTGMPGAIESSEPEYVAEATRKLG
ECCCCCCCHHHHHCCCCCEEEEECCHHHHHHHHEECCCCCCCCCCCCCCHHHHHHHHHHC
LAHVVVTSVDRDDLDDGGAEHFAQTIRAIRERCPATTIEILTPDFLRKDGALEKVVAAKP
HHEEEEEECCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEECHHHHHCCCCHHHHHHCCC
DVFNHNLETVPSRYLTVRPGARYFHSIRLLQRVKEIDPAIFTKSGIMVGLGEERHEVLQV
CHHCCCHHHCCCCEEEECCCHHHHHHHHHHHHHHHCCHHEEECCCEEEECCHHHHHHHHH
MDDLRSADVDFLTIGQYLQPTRKHHAVIRYVTPEEFSSYETVAYTKGFLMVSASPLTRSS
HHHHHHCCCCEEEHHHHHCHHHHCCEEEEEECHHHHCCCCEEEEECCEEEEECCCCCCCC
HHAGEDFAKLQAARATLSR
CCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA