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The map label for this gene is narK2 [H]

Identifier: 75675611

GI number: 75675611

Start: 1545582

End: 1546838

Strand: Direct

Name: narK2 [H]

Synonym: Nwi_1419

Alternate gene names: 75675611

Gene position: 1545582-1546838 (Clockwise)

Preceding gene: 75675610

Following gene: 162139846

Centisome position: 45.43

GC content: 62.61

Gene sequence:

>1257_bases
ATGGCGAGATCCGGCAACCTCTCCGTCCTGCTCATCACGACCTGGGCATTCACCGTTTGCTTCGCTGTGTGGATGATGTT
CGGTGTGATCGGCATTCCGATCCGCGAACAGCTTGGGCTCGGTTCGACGGAATTCGGTCTGCTCACGGCGACGCCTGTCC
TTACCGGCGCATTGTTTCGCTTGCCGCTGGGCATCTGGACCGACCGCTTCGGCGGACGGATCATCATGCTGCTGCTGCTG
GTCGGCTGCGCGGTCCCGGTGTGGCTGTCCGCCTATGCGACCACGCTCTGGCAGTTCCTGCTGCTAGGACTGGCGCTGGG
TCTGGTCGGCGCGTCCTTTTCGGTCGGCACGCCCTATGTCGCGCGCTTCTTTCCCAGGGAACGCCGGGGCTTCGCGATGG
GTTTCTTCGGCGCCGGCACGATCGGCGCCGCCCTGAACATGTTCGTATCTCCCTGGCTCATAGAGGCCTATGGCTGGCAG
ATGGTGCCCAAGGTATATGCCGTCGTGCTGCTGGTCACCGCGCTTCTCTTTTGGGCGTTGTCCGCGCGTGATCCCGGCGT
CGGCAGTCAGGCGCCTTCGATGTGGCGACAATTGTCGGTGCTGCGCGATCCTCGCGTCTGGAAGTATTGCCAGTACTATT
CGATCGTGTTCGGCGGTTTCACCGCGCTGTCGATCTGGATGCCGCAATACTTCAAGACCGAGTACGGTTTCACCATCGCG
CAGGCGTCGCTGCTGGCGGCGTGCTTCTCGCTTCCCGGCGGGGCCTTTCGCGCCTTGGGCGGCTGGCTTTCGGATCGCTT
CGGCGCCCATAACGTCACCTGGTGGGTGCTGTGGGTTGCCTGGGTCTGCCTGTTTCTGCTGTCCTATCCCAGGACGGACC
TGATCGTGCACACGGTCCGTGATCCGCTGAGTTTCAGCATCGCCCTGCCGTCGTGGTTCTTCACCATGCTTCTCTTCACG
CTCGGCATCGCCTTTGCCTGCGGCATGGCCTCGACGTTCAAATACATCGGCGACGACTTTCCCGACAGCATGGGAGCGGT
ATCCGGCGTCGTCGGCATGGCGGGCGGCTTCGGCGGCTTCCTGCTGCCGATCACGTTCGGGGTGATCCTTGACTGGCTCG
GCTTCAACTCGAGCTGCTTCATGCTGCTCTACGGCATCATCTGGGTATCGCTGACGCTCAACTACCTCACCGAAGTCAGG
CAGGCGCCGGTCATGGGAGAGCAAGTTCGATCGCCTGCGGCGGAGCAGTCACGTTAG

Upstream 100 bases:

>100_bases
CCCGAGCCTGCTTATGATGTGACACGGTCGTGAGCCGGGCGGGAAAGGCAGGAACAGCCGAACCCCCGGTTGATCGCGAG
TCAGCATCGGGGCGAACAAT

Downstream 100 bases:

>100_bases
GGTGTTTTCGAGCGACGTGGAAACCGCTTCGCGTGAAGAAAACGCGTCAAAACAATAATCTCTATAGCGTTTTCGAGCGA
AGTGGGTACCGGTTCGCGTG

Product: major facilitator transporter

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 418; Mature: 417

Protein sequence:

>418_residues
MARSGNLSVLLITTWAFTVCFAVWMMFGVIGIPIREQLGLGSTEFGLLTATPVLTGALFRLPLGIWTDRFGGRIIMLLLL
VGCAVPVWLSAYATTLWQFLLLGLALGLVGASFSVGTPYVARFFPRERRGFAMGFFGAGTIGAALNMFVSPWLIEAYGWQ
MVPKVYAVVLLVTALLFWALSARDPGVGSQAPSMWRQLSVLRDPRVWKYCQYYSIVFGGFTALSIWMPQYFKTEYGFTIA
QASLLAACFSLPGGAFRALGGWLSDRFGAHNVTWWVLWVAWVCLFLLSYPRTDLIVHTVRDPLSFSIALPSWFFTMLLFT
LGIAFACGMASTFKYIGDDFPDSMGAVSGVVGMAGGFGGFLLPITFGVILDWLGFNSSCFMLLYGIIWVSLTLNYLTEVR
QAPVMGEQVRSPAAEQSR

Sequences:

>Translated_418_residues
MARSGNLSVLLITTWAFTVCFAVWMMFGVIGIPIREQLGLGSTEFGLLTATPVLTGALFRLPLGIWTDRFGGRIIMLLLL
VGCAVPVWLSAYATTLWQFLLLGLALGLVGASFSVGTPYVARFFPRERRGFAMGFFGAGTIGAALNMFVSPWLIEAYGWQ
MVPKVYAVVLLVTALLFWALSARDPGVGSQAPSMWRQLSVLRDPRVWKYCQYYSIVFGGFTALSIWMPQYFKTEYGFTIA
QASLLAACFSLPGGAFRALGGWLSDRFGAHNVTWWVLWVAWVCLFLLSYPRTDLIVHTVRDPLSFSIALPSWFFTMLLFT
LGIAFACGMASTFKYIGDDFPDSMGAVSGVVGMAGGFGGFLLPITFGVILDWLGFNSSCFMLLYGIIWVSLTLNYLTEVR
QAPVMGEQVRSPAAEQSR
>Mature_417_residues
ARSGNLSVLLITTWAFTVCFAVWMMFGVIGIPIREQLGLGSTEFGLLTATPVLTGALFRLPLGIWTDRFGGRIIMLLLLV
GCAVPVWLSAYATTLWQFLLLGLALGLVGASFSVGTPYVARFFPRERRGFAMGFFGAGTIGAALNMFVSPWLIEAYGWQM
VPKVYAVVLLVTALLFWALSARDPGVGSQAPSMWRQLSVLRDPRVWKYCQYYSIVFGGFTALSIWMPQYFKTEYGFTIAQ
ASLLAACFSLPGGAFRALGGWLSDRFGAHNVTWWVLWVAWVCLFLLSYPRTDLIVHTVRDPLSFSIALPSWFFTMLLFTL
GIAFACGMASTFKYIGDDFPDSMGAVSGVVGMAGGFGGFLLPITFGVILDWLGFNSSCFMLLYGIIWVSLTLNYLTEVRQ
APVMGEQVRSPAAEQSR

Specific function: Involved in excretion of nitrite produced by the dissimilatory reduction of nitrate [H]

COG id: COG2223

COG function: function code P; Nitrate/nitrite transporter

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Nitrate/nitrite porter (TC 2.A.1.8) family [H]

Homologues:

Organism=Escherichia coli, GI2367379, Length=264, Percent_Identity=28.7878787878788, Blast_Score=62, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 46024; Mature: 45893

Theoretical pI: Translated: 8.81; Mature: 8.81

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARSGNLSVLLITTWAFTVCFAVWMMFGVIGIPIREQLGLGSTEFGLLTATPVLTGALFR
CCCCCCEEEEEHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCCHHHHHHHHHHHHHH
LPLGIWTDRFGGRIIMLLLLVGCAVPVWLSAYATTLWQFLLLGLALGLVGASFSVGTPYV
HHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHH
ARFFPRERRGFAMGFFGAGTIGAALNMFVSPWLIEAYGWQMVPKVYAVVLLVTALLFWAL
HHHCCHHHCCCEEEECCCHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHH
SARDPGVGSQAPSMWRQLSVLRDPRVWKYCQYYSIVFGGFTALSIWMPQYFKTEYGFTIA
CCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHH
QASLLAACFSLPGGAFRALGGWLSDRFGAHNVTWWVLWVAWVCLFLLSYPRTDLIVHTVR
HHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHC
DPLSFSIALPSWFFTMLLFTLGIAFACGMASTFKYIGDDFPDSMGAVSGVVGMAGGFGGF
CCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCHH
LLPITFGVILDWLGFNSSCFMLLYGIIWVSLTLNYLTEVRQAPVMGEQVRSPAAEQSR
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCCCCC
>Mature Secondary Structure 
ARSGNLSVLLITTWAFTVCFAVWMMFGVIGIPIREQLGLGSTEFGLLTATPVLTGALFR
CCCCCEEEEEHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCCHHHHHHHHHHHHHH
LPLGIWTDRFGGRIIMLLLLVGCAVPVWLSAYATTLWQFLLLGLALGLVGASFSVGTPYV
HHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHH
ARFFPRERRGFAMGFFGAGTIGAALNMFVSPWLIEAYGWQMVPKVYAVVLLVTALLFWAL
HHHCCHHHCCCEEEECCCHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHH
SARDPGVGSQAPSMWRQLSVLRDPRVWKYCQYYSIVFGGFTALSIWMPQYFKTEYGFTIA
CCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHH
QASLLAACFSLPGGAFRALGGWLSDRFGAHNVTWWVLWVAWVCLFLLSYPRTDLIVHTVR
HHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHC
DPLSFSIALPSWFFTMLLFTLGIAFACGMASTFKYIGDDFPDSMGAVSGVVGMAGGFGGF
CCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCHH
LLPITFGVILDWLGFNSSCFMLLYGIIWVSLTLNYLTEVRQAPVMGEQVRSPAAEQSR
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]