Click here to switch to the map view.

The map label for this gene is irlS [H]

Identifier: 75675391

GI number: 75675391

Start: 1304970

End: 1306430

Strand: Direct

Name: irlS [H]

Synonym: Nwi_1198

Alternate gene names: 75675391

Gene position: 1304970-1306430 (Clockwise)

Preceding gene: 75675389

Following gene: 75675392

Centisome position: 38.36

GC content: 63.52

Gene sequence:

>1461_bases
GTGACTGCGTTCGGCAAGCTGTTCCGCACTACGGCGTTTCGGCTGACGTTGGTCTATTTGCTGTTGTTCGCGCTGTTTGC
GGCTTCTCTTCTGGGATACTTTGCCTGGAATACCCGCCGCCTGATCACCGACCAGATCGCGGCGACAGTGGGCGAGGAAA
TCGGGCTGCTTACCGGCGTCTTCAACCGCCGCGGCTTGCGCGGATTGGTCGGCGCCATCGAAAATCGCGCGCTGCGGCCC
GGCTCCAACCTTTATCTGGTGACGACGCCGGCGGGTCTTGGGGTTGCGGGTAATGTCGGGTCGCTTCAGCCCGGTGTGAT
GGAGACCGCCGGCTGGGCCGAAACCGCCTATCGCAGGCTTGACGAGCAGGATCAGGCTCATCACTACGCCCTGGTGCGGG
TCACCGAACTGTCGAACGGCTTCCGGTTGCTGATTGGCCGCGATCTGGAGGAGCGTCATCGCATTATCGGCGTGGTCGCT
TCGGCCGCGCAATGGTCACTGCTGATCGTCGTGGTCCTGGGGCTTGGCGGCGGCGTCTTTGTGGCGCGGCGCGTGTTGCA
CCGGATCGACGCAATGACCGGTACCACGAAGCGCATCATGGCTGGCGATCTAAGCGGTCGGCTTCCGGTCGGCCGCAGCG
GCGACGAACTGGACCGGCTGGCGGAAAATCTCAATGCTATGCTCGAACGTATCGAAGCGCTTATGAACGGTTTGAGGGAG
GTGTCGGACAATATCGCCCACGACCTCAAAACGCCGTTGACGCGTTTACGCAATCGCGCCGAGGAAGCGCTCGCCAGGTC
CGGCAATGAGAGCGATTATCGCGCGGCCCTGGAACAGACGATCGAGGAATCCGATGGCCTTATCCGCACCTTCAATGCGC
TCCTGATGATCGCTCGCGCGGAATCTGGACATGCACGCGACAACATGGCCGATTTCGATGCCGCGGACGTCGCGGAGGGC
ATTCACGAACTCTATGAGCCGCTGGCCGAAGACAACGGAATGAAGCTCCAGGTTCAGACAGGGTTCGCCCCGCTGCATGG
CAATCGCGAACTGATAAGCCAAGCGCTTGCGAACCTCGTCGAAAACGCGATCAAGTACGGGCAGCCGAAGCCTGCCTCGC
CGCCGCTCGGTGCGGATGCCGTCGCGGCCGACGCCACGATCCTGATCGAAGCCCGCCATGAGGGCGATCGGATCATTCTC
GCCGTGACCGATCACGGTAGCGGGATTCCCGAAGCCGACCGCAAGCGCGCGGTCGAACGCTTCGTGCGGCTCGAAACCAG
CCGGACCCTGGCGGGATCCGGGCTTGGCCTCAGCCTTGCGTCGGCTGTGGCCAGGCTGCACGGCGGGGAGCTTGCGCTCG
ATGATGCGCAGCCGGGTTTGCGCGCCACGCTGTCGATCCCCGCGCGAAGCGCGTCAGGGCTTGCGGCCCCGACGGTGGAC
GTGCAACAGAATGATGCATGA

Upstream 100 bases:

>100_bases
CATGAACTCATTGTCACTTTTGGCCGTCCGTGGCGGATGTTAAGGGAGTTCATCATGGATAAATTCGAGACATGCGAAAC
ACCCTAGACGGATTTGATCC

Downstream 100 bases:

>100_bases
TTTCATCCGTTAAGGACGACGCGGACCCGGCCAGCCTGGCGGCGCGTCTTGCGGACGGACCGAAGCTGTTCGCGCCTTTC
GAAGCCGAGCGCCGGCTTGA

Product: Signal transduction histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 486; Mature: 485

Protein sequence:

>486_residues
MTAFGKLFRTTAFRLTLVYLLLFALFAASLLGYFAWNTRRLITDQIAATVGEEIGLLTGVFNRRGLRGLVGAIENRALRP
GSNLYLVTTPAGLGVAGNVGSLQPGVMETAGWAETAYRRLDEQDQAHHYALVRVTELSNGFRLLIGRDLEERHRIIGVVA
SAAQWSLLIVVVLGLGGGVFVARRVLHRIDAMTGTTKRIMAGDLSGRLPVGRSGDELDRLAENLNAMLERIEALMNGLRE
VSDNIAHDLKTPLTRLRNRAEEALARSGNESDYRAALEQTIEESDGLIRTFNALLMIARAESGHARDNMADFDAADVAEG
IHELYEPLAEDNGMKLQVQTGFAPLHGNRELISQALANLVENAIKYGQPKPASPPLGADAVAADATILIEARHEGDRIIL
AVTDHGSGIPEADRKRAVERFVRLETSRTLAGSGLGLSLASAVARLHGGELALDDAQPGLRATLSIPARSASGLAAPTVD
VQQNDA

Sequences:

>Translated_486_residues
MTAFGKLFRTTAFRLTLVYLLLFALFAASLLGYFAWNTRRLITDQIAATVGEEIGLLTGVFNRRGLRGLVGAIENRALRP
GSNLYLVTTPAGLGVAGNVGSLQPGVMETAGWAETAYRRLDEQDQAHHYALVRVTELSNGFRLLIGRDLEERHRIIGVVA
SAAQWSLLIVVVLGLGGGVFVARRVLHRIDAMTGTTKRIMAGDLSGRLPVGRSGDELDRLAENLNAMLERIEALMNGLRE
VSDNIAHDLKTPLTRLRNRAEEALARSGNESDYRAALEQTIEESDGLIRTFNALLMIARAESGHARDNMADFDAADVAEG
IHELYEPLAEDNGMKLQVQTGFAPLHGNRELISQALANLVENAIKYGQPKPASPPLGADAVAADATILIEARHEGDRIIL
AVTDHGSGIPEADRKRAVERFVRLETSRTLAGSGLGLSLASAVARLHGGELALDDAQPGLRATLSIPARSASGLAAPTVD
VQQNDA
>Mature_485_residues
TAFGKLFRTTAFRLTLVYLLLFALFAASLLGYFAWNTRRLITDQIAATVGEEIGLLTGVFNRRGLRGLVGAIENRALRPG
SNLYLVTTPAGLGVAGNVGSLQPGVMETAGWAETAYRRLDEQDQAHHYALVRVTELSNGFRLLIGRDLEERHRIIGVVAS
AAQWSLLIVVVLGLGGGVFVARRVLHRIDAMTGTTKRIMAGDLSGRLPVGRSGDELDRLAENLNAMLERIEALMNGLREV
SDNIAHDLKTPLTRLRNRAEEALARSGNESDYRAALEQTIEESDGLIRTFNALLMIARAESGHARDNMADFDAADVAEGI
HELYEPLAEDNGMKLQVQTGFAPLHGNRELISQALANLVENAIKYGQPKPASPPLGADAVAADATILIEARHEGDRIILA
VTDHGSGIPEADRKRAVERFVRLETSRTLAGSGLGLSLASAVARLHGGELALDDAQPGLRATLSIPARSASGLAAPTVDV
QQNDA

Specific function: Member of the two-component regulatory system irlR/irlS. May be involved in invasion of eukaryotic cells and heavy-metal resistance. Probably activates irlR by phosphorylation [H]

COG id: COG0642

COG function: function code T; Signal transduction histidine kinase

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1786783, Length=336, Percent_Identity=27.0833333333333, Blast_Score=110, Evalue=3e-25,
Organism=Escherichia coli, GI1787894, Length=309, Percent_Identity=28.1553398058252, Blast_Score=87, Evalue=3e-18,
Organism=Escherichia coli, GI1789808, Length=254, Percent_Identity=28.3464566929134, Blast_Score=86, Evalue=4e-18,
Organism=Escherichia coli, GI1788279, Length=321, Percent_Identity=23.6760124610592, Blast_Score=86, Evalue=4e-18,
Organism=Escherichia coli, GI1790861, Length=251, Percent_Identity=30.6772908366534, Blast_Score=77, Evalue=3e-15,
Organism=Escherichia coli, GI1786912, Length=235, Percent_Identity=28.0851063829787, Blast_Score=75, Evalue=1e-14,
Organism=Escherichia coli, GI1790346, Length=260, Percent_Identity=27.6923076923077, Blast_Score=72, Evalue=5e-14,
Organism=Escherichia coli, GI1788393, Length=293, Percent_Identity=24.2320819112628, Blast_Score=70, Evalue=4e-13,
Organism=Escherichia coli, GI1789403, Length=337, Percent_Identity=24.9258160237389, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1790551, Length=302, Percent_Identity=24.8344370860927, Blast_Score=65, Evalue=8e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR003660
- InterPro:   IPR004358
- InterPro:   IPR006290
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 52246; Mature: 52115

Theoretical pI: Translated: 6.16; Mature: 6.16

Prosite motif: PS50885 HAMP ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAFGKLFRTTAFRLTLVYLLLFALFAASLLGYFAWNTRRLITDQIAATVGEEIGLLTGV
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
FNRRGLRGLVGAIENRALRPGSNLYLVTTPAGLGVAGNVGSLQPGVMETAGWAETAYRRL
HHCCCHHHHHHHHHCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCHHHHCCHHHHHHHHC
DEQDQAHHYALVRVTELSNGFRLLIGRDLEERHRIIGVVASAAQWSLLIVVVLGLGGGVF
CCHHCCHHEEEEEEEECCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHH
VARRVLHRIDAMTGTTKRIMAGDLSGRLPVGRSGDELDRLAENLNAMLERIEALMNGLRE
HHHHHHHHHHHHCCCHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
VSDNIAHDLKTPLTRLRNRAEEALARSGNESDYRAALEQTIEESDGLIRTFNALLMIARA
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHEEC
ESGHARDNMADFDAADVAEGIHELYEPLAEDNGMKLQVQTGFAPLHGNRELISQALANLV
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCHHHHHHHHHHHH
ENAIKYGQPKPASPPLGADAVAADATILIEARHEGDRIILAVTDHGSGIPEADRKRAVER
HHHHHCCCCCCCCCCCCCCHHHCCEEEEEEEECCCCEEEEEEECCCCCCCCHHHHHHHHH
FVRLETSRTLAGSGLGLSLASAVARLHGGELALDDAQPGLRATLSIPARSASGLAAPTVD
HHHHHHCCHHCCCCCHHHHHHHHHHHCCCCEEECCCCCCCEEEEECCCCCCCCCCCCCCC
VQQNDA
CCCCCC
>Mature Secondary Structure 
TAFGKLFRTTAFRLTLVYLLLFALFAASLLGYFAWNTRRLITDQIAATVGEEIGLLTGV
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
FNRRGLRGLVGAIENRALRPGSNLYLVTTPAGLGVAGNVGSLQPGVMETAGWAETAYRRL
HHCCCHHHHHHHHHCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCHHHHCCHHHHHHHHC
DEQDQAHHYALVRVTELSNGFRLLIGRDLEERHRIIGVVASAAQWSLLIVVVLGLGGGVF
CCHHCCHHEEEEEEEECCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHH
VARRVLHRIDAMTGTTKRIMAGDLSGRLPVGRSGDELDRLAENLNAMLERIEALMNGLRE
HHHHHHHHHHHHCCCHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
VSDNIAHDLKTPLTRLRNRAEEALARSGNESDYRAALEQTIEESDGLIRTFNALLMIARA
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHEEC
ESGHARDNMADFDAADVAEGIHELYEPLAEDNGMKLQVQTGFAPLHGNRELISQALANLV
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCHHHHHHHHHHHH
ENAIKYGQPKPASPPLGADAVAADATILIEARHEGDRIILAVTDHGSGIPEADRKRAVER
HHHHHCCCCCCCCCCCCCCHHHCCEEEEEEEECCCCEEEEEEECCCCCCCCHHHHHHHHH
FVRLETSRTLAGSGLGLSLASAVARLHGGELALDDAQPGLRATLSIPARSASGLAAPTVD
HHHHHHCCHHCCCCCHHHHHHHHHHHCCCCEEECCCCCCCEEEEECCCCCCCCCCCCCCC
VQQNDA
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9393784 [H]