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The map label for this gene is dksA [H]

Identifier: 75675306

GI number: 75675306

Start: 1224098

End: 1224508

Strand: Direct

Name: dksA [H]

Synonym: Nwi_1113

Alternate gene names: 75675306

Gene position: 1224098-1224508 (Clockwise)

Preceding gene: 75675305

Following gene: 75675316

Centisome position: 35.98

GC content: 57.91

Gene sequence:

>411_bases
TTGGAAAAGCTGAAGAACTACAGGCCTTCTGAGAAAGAGCCTTTCATGAACGAGCGCCAGCGCGAATATTTCCGCGCCAA
GCTGCTGGCATGGAAGGACGAGATTCTTCGCGAATCGAAGGTGACGCTGCAGACTCTTCAAGAAGAAAACGTCAACCTTC
CCGACCTTGCCGATCGCGCGTCCTCGGAGACCGACCGCGCTATCGAGCTGCGCGCCCGTGACCGGCAGCGAAAGCTGATC
TCGAAAATCGACGCCGCCTTGCAGCGGATCGAGGACAACACCTATGGCTATTGCGAGGAAACGGGCGAGCCGATTTCATT
GAAGCGGCTCGAGGCGCGCCCGATCGCGACGCTTTCCGTCGAAGCCCAGGAGCGTCACGAGAAGCGGGAAAAGATCTATC
GGGACGAATAG

Upstream 100 bases:

>100_bases
GGGGTGGACTTCGGGACGACGATATTGTTTGTCACAAACGGCCGCTATATAAGCTCGCCGCAGCCGGATCACCCGGCGTT
TTCACCGAGCAGGAACGCAT

Downstream 100 bases:

>100_bases
GGCGGCTGCTCCGGCGCGGGGTCGCGGCCTCTATAGCGTTTTCGTCGCTGGTTGAATCAGAATCGTGCGGCGCTCCGCGG
AGCGGCGGCCCAGGGCCGCC

Product: DnaK suppressor protein DksA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 136; Mature: 136

Protein sequence:

>136_residues
MEKLKNYRPSEKEPFMNERQREYFRAKLLAWKDEILRESKVTLQTLQEENVNLPDLADRASSETDRAIELRARDRQRKLI
SKIDAALQRIEDNTYGYCEETGEPISLKRLEARPIATLSVEAQERHEKREKIYRDE

Sequences:

>Translated_136_residues
MEKLKNYRPSEKEPFMNERQREYFRAKLLAWKDEILRESKVTLQTLQEENVNLPDLADRASSETDRAIELRARDRQRKLI
SKIDAALQRIEDNTYGYCEETGEPISLKRLEARPIATLSVEAQERHEKREKIYRDE
>Mature_136_residues
MEKLKNYRPSEKEPFMNERQREYFRAKLLAWKDEILRESKVTLQTLQEENVNLPDLADRASSETDRAIELRARDRQRKLI
SKIDAALQRIEDNTYGYCEETGEPISLKRLEARPIATLSVEAQERHEKREKIYRDE

Specific function: Dosage-Dependent Suppressor Of A Dnak Deletion Mutation. It Suppressed Not Only The Temperature-Sensitive Growth But Also The Filamentous Phenotype Of The Dnak Deletion Strain, While The Defect Of Lambda Growth Is Not Suppressed. [C]

COG id: COG1734

COG function: function code T; DnaK suppressor protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 dksA C4-type zinc finger [H]

Homologues:

Organism=Escherichia coli, GI1786338, Length=125, Percent_Identity=44, Blast_Score=108, Evalue=1e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012784
- InterPro:   IPR000962
- InterPro:   IPR020458 [H]

Pfam domain/function: PF01258 zf-dskA_traR [H]

EC number: NA

Molecular weight: Translated: 16239; Mature: 16239

Theoretical pI: Translated: 6.08; Mature: 6.08

Prosite motif: PS01102 ZF_DKSA_1 ; PS51128 ZF_DKSA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKLKNYRPSEKEPFMNERQREYFRAKLLAWKDEILRESKVTLQTLQEENVNLPDLADRA
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHC
SSETDRAIELRARDRQRKLISKIDAALQRIEDNTYGYCEETGEPISLKRLEARPIATLSV
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHCCCCCEEEEC
EAQERHEKREKIYRDE
HHHHHHHHHHHHCCCC
>Mature Secondary Structure
MEKLKNYRPSEKEPFMNERQREYFRAKLLAWKDEILRESKVTLQTLQEENVNLPDLADRA
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHC
SSETDRAIELRARDRQRKLISKIDAALQRIEDNTYGYCEETGEPISLKRLEARPIATLSV
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHCCCCCEEEEC
EAQERHEKREKIYRDE
HHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9555902 [H]