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The map label for this gene is mraW

Identifier: 75675236

GI number: 75675236

Start: 1136258

End: 1137256

Strand: Direct

Name: mraW

Synonym: Nwi_1043

Alternate gene names: 75675236

Gene position: 1136258-1137256 (Clockwise)

Preceding gene: 75675234

Following gene: 75675237

Centisome position: 33.4

GC content: 69.57

Gene sequence:

>999_bases
ATGACGGCGCCGCCGATTCGTCATGTGCCCGTGCTCGGCCGGGAAGCCGTCGCCATGTTGGCGCCGCGGGCCGGCGGCGT
CTACGTCGATGCCACGTTCGGGGCCGGAGGCTATTCGCGCGCCATTCTCGCAACCGCGGAGACCCGCGTGATCGGGATCG
ACCGCGACCCAACCGCCATTGCCGGCGGCTCCGGCCTGGTGGAGGAGTCCGGCGGCCGGTTGATATTGGTCGAGGATCGC
TTCTCTCATCTCGCCGCGGTCTGCGCGGCGCAGGACGCCGCCGCGGTGGATGGCGTTGTCATGGATGTCGGCGTCTCCTC
GATGCAGCTGGACGAGGCCGGTCGGGGCTTCTCCTTTCGTCTCGACGGACCGCTCGACATGCGCATGAGCGGCCATGGGC
CGACCGCGGCCGAGGTCGTGGCCCGCGCCTCCGAGACCGATCTCGCCGATATCATCTACATCTTCGGCGAAGAGCGTCGC
TCGCGCGCCGTCGCCCGCGCCATCGTCGGGGCACGGCGACACGGGCCGATCGCGACCACGCGGGCGCTGGCCGATATCGT
CTCGAAGGTGGTTCGCGCGAAGCCGCACGAGATTCATCCGGCGACGCGGACGTTTCAGGCTCTGCGGATCTTCGTCAACG
AGGAACTTGACGAACTGATCGCGGCGCTGGCCGCGGCGGAACGCGTGCTGAAGCCAGGCGGCCGGCTAGCTGTGGTGTCC
TTTCACTCGCTGGAAGACCGCATCGTCAAGAATTTCCTGGCCTGGCGCGGCAAGACGGGCGGCGGCTCGCGCCATCGGCC
CGAAATCGAACGCGCGCCGCCGAGCTTTGCCATTCTGACCAAGCGTCCGCTGACGCCGGGTGACGACGAAGTCCAGGCCA
ACCCGCGCGCGCGCTCCGCCAGGCTGCGCGCCGCCGAGCGTACCGATGCGGCCGCGATTGATGACGGCGCCGAGCTTCCG
GCCTGGCCGTCGCTCGCCAACGTGATGCGCGGAGGCTGA

Upstream 100 bases:

>100_bases
CCGGCTTCAGGGTGGGAGCGGGCGGCTGAATTCCGGACGGTCGGGATCGGATGAACGCACGCGTGGCGAGAGCGAGGCAT
CTGCGGATGGAGGCGCGATC

Downstream 100 bases:

>100_bases
TGAGGTGCGGATCATCCATTTCCTCGTCGTCTGCGCGCTGGTCTATGCGGCATCGTATGTCTACCGCATCAAGATGGATT
CGACCTCCCGCACCGAGCGT

Product: S-adenosyl-methyltransferase MraW

Products: NA

Alternate protein names: 16S rRNA m(4)C1402 methyltransferase; rRNA (cytosine-N(4)-)-methyltransferase RsmH

Number of amino acids: Translated: 332; Mature: 331

Protein sequence:

>332_residues
MTAPPIRHVPVLGREAVAMLAPRAGGVYVDATFGAGGYSRAILATAETRVIGIDRDPTAIAGGSGLVEESGGRLILVEDR
FSHLAAVCAAQDAAAVDGVVMDVGVSSMQLDEAGRGFSFRLDGPLDMRMSGHGPTAAEVVARASETDLADIIYIFGEERR
SRAVARAIVGARRHGPIATTRALADIVSKVVRAKPHEIHPATRTFQALRIFVNEELDELIAALAAAERVLKPGGRLAVVS
FHSLEDRIVKNFLAWRGKTGGGSRHRPEIERAPPSFAILTKRPLTPGDDEVQANPRARSARLRAAERTDAAAIDDGAELP
AWPSLANVMRGG

Sequences:

>Translated_332_residues
MTAPPIRHVPVLGREAVAMLAPRAGGVYVDATFGAGGYSRAILATAETRVIGIDRDPTAIAGGSGLVEESGGRLILVEDR
FSHLAAVCAAQDAAAVDGVVMDVGVSSMQLDEAGRGFSFRLDGPLDMRMSGHGPTAAEVVARASETDLADIIYIFGEERR
SRAVARAIVGARRHGPIATTRALADIVSKVVRAKPHEIHPATRTFQALRIFVNEELDELIAALAAAERVLKPGGRLAVVS
FHSLEDRIVKNFLAWRGKTGGGSRHRPEIERAPPSFAILTKRPLTPGDDEVQANPRARSARLRAAERTDAAAIDDGAELP
AWPSLANVMRGG
>Mature_331_residues
TAPPIRHVPVLGREAVAMLAPRAGGVYVDATFGAGGYSRAILATAETRVIGIDRDPTAIAGGSGLVEESGGRLILVEDRF
SHLAAVCAAQDAAAVDGVVMDVGVSSMQLDEAGRGFSFRLDGPLDMRMSGHGPTAAEVVARASETDLADIIYIFGEERRS
RAVARAIVGARRHGPIATTRALADIVSKVVRAKPHEIHPATRTFQALRIFVNEELDELIAALAAAERVLKPGGRLAVVSF
HSLEDRIVKNFLAWRGKTGGGSRHRPEIERAPPSFAILTKRPLTPGDDEVQANPRARSARLRAAERTDAAAIDDGAELPA
WPSLANVMRGG

Specific function: Specifically methylates the N4 position of cytidine in position 1402 (C1402) of 16S rRNA

COG id: COG0275

COG function: function code M; Predicted S-adenosylmethionine-dependent methyltransferase involved in cell envelope biogenesis

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmH family

Homologues:

Organism=Homo sapiens, GI165377209, Length=333, Percent_Identity=43.8438438438438, Blast_Score=234, Evalue=6e-62,
Organism=Homo sapiens, GI165377202, Length=189, Percent_Identity=43.3862433862434, Blast_Score=141, Evalue=8e-34,
Organism=Escherichia coli, GI1786270, Length=318, Percent_Identity=45.2830188679245, Blast_Score=236, Evalue=1e-63,
Organism=Drosophila melanogaster, GI62472493, Length=336, Percent_Identity=42.2619047619048, Blast_Score=215, Evalue=4e-56,
Organism=Drosophila melanogaster, GI28571637, Length=336, Percent_Identity=42.2619047619048, Blast_Score=215, Evalue=4e-56,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RSMH_NITWN (Q3STT6)

Other databases:

- EMBL:   CP000115
- RefSeq:   YP_317657.1
- ProteinModelPortal:   Q3STT6
- SMR:   Q3STT6
- STRING:   Q3STT6
- GeneID:   3675785
- GenomeReviews:   CP000115_GR
- KEGG:   nwi:Nwi_1043
- NMPDR:   fig|323098.3.peg.855
- eggNOG:   COG0275
- HOGENOM:   HBG302779
- OMA:   PQLDDPE
- PhylomeDB:   Q3STT6
- ProtClustDB:   PRK00050
- BioCyc:   NWIN323098:NWI_1043-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01007
- InterPro:   IPR002903
- PANTHER:   PTHR11265
- PIRSF:   PIRSF004486
- TIGRFAMs:   TIGR00006

Pfam domain/function: PF01795 Methyltransf_5

EC number: NA

Molecular weight: Translated: 35177; Mature: 35045

Theoretical pI: Translated: 8.52; Mature: 8.52

Prosite motif: NA

Important sites: BINDING 54-54 BINDING 81-81 BINDING 102-102 BINDING 109-109

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAPPIRHVPVLGREAVAMLAPRAGGVYVDATFGAGGYSRAILATAETRVIGIDRDPTAI
CCCCCCCCCCCCCHHHHHHHCCCCCCEEEEEEECCCCCCCEEEEECCEEEEEECCCCCEE
AGGSGLVEESGGRLILVEDRFSHLAAVCAAQDAAAVDGVVMDVGVSSMQLDEAGRGFSFR
ECCCCCEECCCCEEEEEECHHHHHHHHHHHCCHHHHCCEEEECCCCCEEHHCCCCCEEEE
LDGPLDMRMSGHGPTAAEVVARASETDLADIIYIFGEERRSRAVARAIVGARRHGPIATT
ECCCEEEEECCCCCHHHHHHHHCCCCCHHHEEEEECCCHHHHHHHHHHHHHHCCCCHHHH
RALADIVSKVVRAKPHEIHPATRTFQALRIFVNEELDELIAALAAAERVLKPGGRLAVVS
HHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEE
FHSLEDRIVKNFLAWRGKTGGGSRHRPEIERAPPSFAILTKRPLTPGDDEVQANPRARSA
EHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHCCCCEEEEECCCCCCCCCCCCCCCCHHHH
RLRAAERTDAAAIDDGAELPAWPSLANVMRGG
HHHHHHCCCCCCCCCCCCCCCCHHHHHHHCCC
>Mature Secondary Structure 
TAPPIRHVPVLGREAVAMLAPRAGGVYVDATFGAGGYSRAILATAETRVIGIDRDPTAI
CCCCCCCCCCCCHHHHHHHCCCCCCEEEEEEECCCCCCCEEEEECCEEEEEECCCCCEE
AGGSGLVEESGGRLILVEDRFSHLAAVCAAQDAAAVDGVVMDVGVSSMQLDEAGRGFSFR
ECCCCCEECCCCEEEEEECHHHHHHHHHHHCCHHHHCCEEEECCCCCEEHHCCCCCEEEE
LDGPLDMRMSGHGPTAAEVVARASETDLADIIYIFGEERRSRAVARAIVGARRHGPIATT
ECCCEEEEECCCCCHHHHHHHHCCCCCHHHEEEEECCCHHHHHHHHHHHHHHCCCCHHHH
RALADIVSKVVRAKPHEIHPATRTFQALRIFVNEELDELIAALAAAERVLKPGGRLAVVS
HHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEE
FHSLEDRIVKNFLAWRGKTGGGSRHRPEIERAPPSFAILTKRPLTPGDDEVQANPRARSA
EHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHCCCCEEEEECCCCCCCCCCCCCCCCHHHH
RLRAAERTDAAAIDDGAELPAWPSLANVMRGG
HHHHHHCCCCCCCCCCCCCCCCHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA