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The map label for this gene is yafC [C]

Identifier: 75675173

GI number: 75675173

Start: 1074831

End: 1075745

Strand: Direct

Name: yafC [C]

Synonym: Nwi_0980

Alternate gene names: 75675173

Gene position: 1074831-1075745 (Clockwise)

Preceding gene: 75675168

Following gene: 75675174

Centisome position: 31.59

GC content: 66.34

Gene sequence:

>915_bases
ATGGCGAAGCTTCCGGATTTCGAGGCGCTGGCGATCTTCGCCAAGGTTGTCGAGCTGCGCTCCTTCGCCGGCGCGGCGGC
TGATCTGGGCCTGTCGAAAGCAACGGTTTCCAAAGCCGTCAGCCGGCTAGAGGACCGGCTCGGCGCCCGTCTCTTCAACA
GAACATCGCGCCGCCTGGCGCTGACGGATGCGGGCAAGAACCTGTCGGAACGCGCCGCGCGTCTGCTCGCGGACGGCGAG
GCGGCCGAGAACGAGGCGCTGGCGCGATCGACGGTGCCGCGCGGCCTGGTGCGGCTTGCGGTTCCCATGACGTTCGGTGT
CAACGTGATCGCGCCGCTACTGCCCGGCTTCCTCGCGATGTATCCCGAGGTGTCGGTGGATCTTCATTTCAGCGACGCGA
TGGTCGATCTGATCGGCGAGGGGTTTGACGTAGGCGTGCGGATTGCCAGCCTGCCGGATTCGTCATTGCTGGCGCGGCGG
CTCTGCCCGATGCCGCGTCATACCGTTGCGTCGCCGGCCTATCTCAAGGCGCACGGCAGGCCGACGCACCCGATGCATCT
CGCCCAGCACAGTTGCCTGGGCTACGCTTATCTGTCGACGCCGGGCGTATGGCGTTACGTCAATGCGCGCGGCGAACAGG
CTGCCGTGCGGCCATCGGGCCGACTGCGCGTCAATAACGGCGAGGCGCTGCTGCCCGCCATCATCGCCGGACTTGGGATA
GCGGACCTGCCGGAATTCATCATCGGCGACGCCATCGCCGCGAAACAGGTCGAGGTTCTCCTGAAGGGTTGGAAACAGGT
GGAAGGCGCGGTTCATCTGGTGATGCCGCCGGGCGGACCGCGACCGGCGCGCATCGAGGTGCTGATCGATTACCTCGCCC
ATCAACTCGCGCATCAAGGGCGGCGCGGAGGATGA

Upstream 100 bases:

>100_bases
CCGGTTGCGGTTGACGGATGGATATGTCTTTCGAGTATGTGTATAAATAGAAACTATCGAAACGCATTGTTTCTAAATAT
TACCAATTGCGGGACAGGGA

Downstream 100 bases:

>100_bases
GAGTTTCTTCGCGCGACATAGCGCAGCGTATGCGGGGTGCGGGTGGGACCGGACGAGGGGCGGGCCCGCCGCGAAAAGCC
ACACAAACCCTCATTGTATG

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: ORF2 [H]

Number of amino acids: Translated: 304; Mature: 303

Protein sequence:

>304_residues
MAKLPDFEALAIFAKVVELRSFAGAAADLGLSKATVSKAVSRLEDRLGARLFNRTSRRLALTDAGKNLSERAARLLADGE
AAENEALARSTVPRGLVRLAVPMTFGVNVIAPLLPGFLAMYPEVSVDLHFSDAMVDLIGEGFDVGVRIASLPDSSLLARR
LCPMPRHTVASPAYLKAHGRPTHPMHLAQHSCLGYAYLSTPGVWRYVNARGEQAAVRPSGRLRVNNGEALLPAIIAGLGI
ADLPEFIIGDAIAAKQVEVLLKGWKQVEGAVHLVMPPGGPRPARIEVLIDYLAHQLAHQGRRGG

Sequences:

>Translated_304_residues
MAKLPDFEALAIFAKVVELRSFAGAAADLGLSKATVSKAVSRLEDRLGARLFNRTSRRLALTDAGKNLSERAARLLADGE
AAENEALARSTVPRGLVRLAVPMTFGVNVIAPLLPGFLAMYPEVSVDLHFSDAMVDLIGEGFDVGVRIASLPDSSLLARR
LCPMPRHTVASPAYLKAHGRPTHPMHLAQHSCLGYAYLSTPGVWRYVNARGEQAAVRPSGRLRVNNGEALLPAIIAGLGI
ADLPEFIIGDAIAAKQVEVLLKGWKQVEGAVHLVMPPGGPRPARIEVLIDYLAHQLAHQGRRGG
>Mature_303_residues
AKLPDFEALAIFAKVVELRSFAGAAADLGLSKATVSKAVSRLEDRLGARLFNRTSRRLALTDAGKNLSERAARLLADGEA
AENEALARSTVPRGLVRLAVPMTFGVNVIAPLLPGFLAMYPEVSVDLHFSDAMVDLIGEGFDVGVRIASLPDSSLLARRL
CPMPRHTVASPAYLKAHGRPTHPMHLAQHSCLGYAYLSTPGVWRYVNARGEQAAVRPSGRLRVNNGEALLPAIIAGLGIA
DLPEFIIGDAIAAKQVEVLLKGWKQVEGAVHLVMPPGGPRPARIEVLIDYLAHQLAHQGRRGG

Specific function: Unknown

COG id: COG0583

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1786401, Length=290, Percent_Identity=35.5172413793103, Blast_Score=164, Evalue=6e-42,
Organism=Escherichia coli, GI1789639, Length=292, Percent_Identity=32.1917808219178, Blast_Score=157, Evalue=1e-39,
Organism=Escherichia coli, GI1787128, Length=295, Percent_Identity=28.135593220339, Blast_Score=138, Evalue=6e-34,
Organism=Escherichia coli, GI145693193, Length=287, Percent_Identity=31.0104529616725, Blast_Score=134, Evalue=9e-33,
Organism=Escherichia coli, GI87081978, Length=300, Percent_Identity=31, Blast_Score=130, Evalue=8e-32,
Organism=Escherichia coli, GI1789440, Length=232, Percent_Identity=31.4655172413793, Blast_Score=125, Evalue=4e-30,
Organism=Escherichia coli, GI1787589, Length=297, Percent_Identity=29.96632996633, Blast_Score=124, Evalue=6e-30,
Organism=Escherichia coli, GI157672245, Length=140, Percent_Identity=34.2857142857143, Blast_Score=73, Evalue=3e-14,
Organism=Escherichia coli, GI1789173, Length=196, Percent_Identity=28.5714285714286, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1788748, Length=250, Percent_Identity=26.8, Blast_Score=66, Evalue=2e-12,
Organism=Escherichia coli, GI2367136, Length=298, Percent_Identity=27.5167785234899, Blast_Score=63, Evalue=2e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 32514; Mature: 32383

Theoretical pI: Translated: 10.02; Mature: 10.02

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKLPDFEALAIFAKVVELRSFAGAAADLGLSKATVSKAVSRLEDRLGARLFNRTSRRLA
CCCCCCHHHHHHHHHHHHHHHHCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEE
LTDAGKNLSERAARLLADGEAAENEALARSTVPRGLVRLAVPMTFGVNVIAPLLPGFLAM
EECCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YPEVSVDLHFSDAMVDLIGEGFDVGVRIASLPDSSLLARRLCPMPRHTVASPAYLKAHGR
CCCCEEEEEHHHHHHHHHCCCCCCCEEEECCCCHHHHHHHHCCCCCCCCCCCHHEECCCC
PTHPMHLAQHSCLGYAYLSTPGVWRYVNARGEQAAVRPSGRLRVNNGEALLPAIIAGLGI
CCCCHHHHHHHHHEEEEECCCCEEEEECCCCCCEEECCCCCEEECCCCHHHHHHHHHCCH
ADLPEFIIGDAIAAKQVEVLLKGWKQVEGAVHLVMPPGGPRPARIEVLIDYLAHQLAHQG
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHCC
RRGG
CCCC
>Mature Secondary Structure 
AKLPDFEALAIFAKVVELRSFAGAAADLGLSKATVSKAVSRLEDRLGARLFNRTSRRLA
CCCCCHHHHHHHHHHHHHHHHCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEE
LTDAGKNLSERAARLLADGEAAENEALARSTVPRGLVRLAVPMTFGVNVIAPLLPGFLAM
EECCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YPEVSVDLHFSDAMVDLIGEGFDVGVRIASLPDSSLLARRLCPMPRHTVASPAYLKAHGR
CCCCEEEEEHHHHHHHHHCCCCCCCEEEECCCCHHHHHHHHCCCCCCCCCCCHHEECCCC
PTHPMHLAQHSCLGYAYLSTPGVWRYVNARGEQAAVRPSGRLRVNNGEALLPAIIAGLGI
CCCCHHHHHHHHHEEEEECCCCEEEEECCCCCCEEECCCCCEEECCCCHHHHHHHHHCCH
ADLPEFIIGDAIAAKQVEVLLKGWKQVEGAVHLVMPPGGPRPARIEVLIDYLAHQLAHQG
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHCC
RRGG
CCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8635745; 7542800 [H]