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The map label for this gene is ntaB [H]

Identifier: 75675131

GI number: 75675131

Start: 1028083

End: 1028625

Strand: Direct

Name: ntaB [H]

Synonym: Nwi_0938

Alternate gene names: 75675131

Gene position: 1028083-1028625 (Clockwise)

Preceding gene: 75675130

Following gene: 75675132

Centisome position: 30.22

GC content: 61.69

Gene sequence:

>543_bases
ATGACTGACGGTTCTGAACTTCCCGACACGCCGGATCCGGCTCGTGAACTGGCGAGCGGGGAGTCGACTCTCGATCCGCG
CGACTTCCGCAATGCGCTCGGAACCTATGCGACGGGCGTCACCATCATCACGGCTGTCGACGAGGAAGGAACGCCGCACG
GGTTAACGTGCAACTCGTTCGCGTCGGTCTCGCTGAATCCGCCGCTGGTGCTGTGGAGCCTCGTGATCTATTCGCGGAAC
ATGAGCGTCTTTCAAAACGCCAGCCATTTCGCCGTCAATGTGCTCGGGGCTTCACAACAAGGGCTCGCGACCAAATTCGC
GCAGCCGTCGAGCCGGAAGTTCGAGGGGGTCGGCTGGAAACCGGGTCTCGGCGGCGCTCCGCTTCTGGCGGGCAGCGTTG
CGAGCTTTGAATGTCGAGCCGTCGATCGTTATTACGGCGGAGATCACGTCATCTTCCTCGGCGCCGTGGAAGCCTATAGC
TACAACCGGGACGAGGCGCTGTTGTTTTCCCGCGGCAAGTATGGCCGCTTCGTCGGCGAATGA

Upstream 100 bases:

>100_bases
TCAACTCCCATATCGCATTCAGCTTCGATGCTGCGGGCACGGATTACGGCCGGGTCGCCCTCGGGCTGCCGTCGCAGAAT
CTGACGTTGTGAGGCGTCTG

Downstream 100 bases:

>100_bases
CGGCCTGCTGGCCCTGATGCGCAAGCAGCCTGAATTCCGGCGATGTCTCGCAAGCCGGCATGTCGGATGCCCTTGCCGCG
GCGCTTCCAGCCCGACTTTC

Product: flavin reductase-like, FMN-binding

Products: NAD(+); reduced flavin [C]

Alternate protein names: NTA monooxygenase component B; NTA-MO B [H]

Number of amino acids: Translated: 180; Mature: 179

Protein sequence:

>180_residues
MTDGSELPDTPDPARELASGESTLDPRDFRNALGTYATGVTIITAVDEEGTPHGLTCNSFASVSLNPPLVLWSLVIYSRN
MSVFQNASHFAVNVLGASQQGLATKFAQPSSRKFEGVGWKPGLGGAPLLAGSVASFECRAVDRYYGGDHVIFLGAVEAYS
YNRDEALLFSRGKYGRFVGE

Sequences:

>Translated_180_residues
MTDGSELPDTPDPARELASGESTLDPRDFRNALGTYATGVTIITAVDEEGTPHGLTCNSFASVSLNPPLVLWSLVIYSRN
MSVFQNASHFAVNVLGASQQGLATKFAQPSSRKFEGVGWKPGLGGAPLLAGSVASFECRAVDRYYGGDHVIFLGAVEAYS
YNRDEALLFSRGKYGRFVGE
>Mature_179_residues
TDGSELPDTPDPARELASGESTLDPRDFRNALGTYATGVTIITAVDEEGTPHGLTCNSFASVSLNPPLVLWSLVIYSRNM
SVFQNASHFAVNVLGASQQGLATKFAQPSSRKFEGVGWKPGLGGAPLLAGSVASFECRAVDRYYGGDHVIFLGAVEAYSY
NRDEALLFSRGKYGRFVGE

Specific function: Hydroxylation of nitrilotriacetate [H]

COG id: COG1853

COG function: function code R; Conserved protein/domain typically associated with flavoprotein oxygenases, DIM6/NTAB family

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI87081819, Length=140, Percent_Identity=32.1428571428571, Blast_Score=64, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002563
- InterPro:   IPR012349
- InterPro:   IPR009002
- InterPro:   IPR011991 [H]

Pfam domain/function: PF01613 Flavin_Reduct [H]

EC number: 1.6.8.- [C]

Molecular weight: Translated: 19245; Mature: 19113

Theoretical pI: Translated: 4.93; Mature: 4.93

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDGSELPDTPDPARELASGESTLDPRDFRNALGTYATGVTIITAVDEEGTPHGLTCNSF
CCCCCCCCCCCCHHHHHHCCCCCCCHHHHHHHHHHHHCCEEEEEEECCCCCCCCEECCCE
ASVSLNPPLVLWSLVIYSRNMSVFQNASHFAVNVLGASQQGLATKFAQPSSRKFEGVGWK
EEECCCCHHHHHHHHHHHCCHHHHHCCCCEEEEEECCCCCCCHHHCCCCCCCCCCCCCCC
PGLGGAPLLAGSVASFECRAVDRYYGGDHVIFLGAVEAYSYNRDEALLFSRGKYGRFVGE
CCCCCCCEEECCCCCEEEEEEHHEECCCEEEEEEEEEEECCCCCCEEEEECCCCCCCCCC
>Mature Secondary Structure 
TDGSELPDTPDPARELASGESTLDPRDFRNALGTYATGVTIITAVDEEGTPHGLTCNSF
CCCCCCCCCCCHHHHHHCCCCCCCHHHHHHHHHHHHCCEEEEEEECCCCCCCCEECCCE
ASVSLNPPLVLWSLVIYSRNMSVFQNASHFAVNVLGASQQGLATKFAQPSSRKFEGVGWK
EEECCCCHHHHHHHHHHHCCHHHHHCCCCEEEEEECCCCCCCHHHCCCCCCCCCCCCCCC
PGLGGAPLLAGSVASFECRAVDRYYGGDHVIFLGAVEAYSYNRDEALLFSRGKYGRFVGE
CCCCCCCEEECCCCCEEEEEEHHEECCCEEEEEEEEEEECCCCCCEEEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADH; flavin [C]

Specific reaction: NADH + flavin = NAD(+) + reduced flavin [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8892809; 9023192; 1735711 [H]