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The map label for this gene is dfrA [H]

Identifier: 75675093

GI number: 75675093

Start: 976580

End: 977608

Strand: Direct

Name: dfrA [H]

Synonym: Nwi_0898

Alternate gene names: 75675093

Gene position: 976580-977608 (Clockwise)

Preceding gene: 75675092

Following gene: 75675094

Centisome position: 28.71

GC content: 66.57

Gene sequence:

>1029_bases
ATGCTGCAACCTCGCGCCAGAATGGCGCTTGCTGGCCGTCGCCCGATCCTGGTCACAGGTGGAAATGGATTCATCGGACA
ACATCTGGTTGCAGCGCTGCGCCGCAGGCACGGCGTCGTGCGCGTCCTGGATCTGCAACCGCCGCCACCCGGACCCGTGC
CGGAATTCATCCAGGGAACGATCCTGGATCCGCACGATGTGCGGCGCGCTCTGGACGGCGTGGATACCGTGTATCATCTG
GCGGCGATCTCGCATCTGTGGACGGCGAACCCCGCCGACTTCGAGCGCGTGAATCAACATGGCACCGAACTCATGCTAGC
GGCGGCGCGCGAGAGAGGGGTGCGCAACATCGTTCACTGCTCCACCGAAGCGATCCTGTTTCCCTATCGCAGGGGCGGCA
CAACGATGCCGCAACGCGCGGAAGACATGCCGGGGCCCTATACGCGATCGAAGTTCATGGCCGAGCAGATCGCGCGCGAG
GCGGCGGCCGATGGACTGCGTGTCGTGATCGCCAATCCCACCGTTCCCATCGGGCCCGGCGATCACAACTTTACCGAGCC
GACGCGGATGCTCGACCTGTTCGCGCGCAAATCGCCGCCGATGGTGCTGGACTCGATGCTGAACCTCGTGGACGTGCGGG
ACGTCGCGGCGGGGCTCATCCTGGCCGGAGAGCGCGGCAGGGCGGGAGAGCGCTATATTCTCGGCGGCGAAAATGTCAGC
GTGCGCGAACTCGTGCGGCGTGTCGGAAGCCTGTGCGGCCGCAGCGCCAACGTGCATGCGTTGCCGGCTTCGCTGGCGCT
TGCGATCGGAGCGGCGTCCGAATGGTTCGAAGGCCAGGTGATCCAGCGCACGCCGCGGGTCTCGATCGAGGCGGTGCGCA
TCGCGTTGCGCTCGATCCCGCTGGACACACGAAAGGCGGAGACCGAACTCGGCTATCTGCCTCGCCCGATCGACGACGCG
CTCACCGACGCCATCGCCTGGCTCACGCGCCGTGACGCGGATGCGGCTCAGCCGCGGGTCTCGGCTTAG

Upstream 100 bases:

>100_bases
ATCCGGAAAGCATTCGGGTCATTGAAGGTTGGAACGGCCCGCCGGTGTAGTATCCTTATCCTACCGGGTCGATCTCCTTG
GAGCCGCGGACGTGAAATCC

Downstream 100 bases:

>100_bases
AGCCTGTTTCAACCGCGTTGAATCCGATGCAACCGCGTTGAATCAGAAGCGGACACCGGTTCGCGTAAAGAAAACGCGAC
AAAACAAAATCAGAGAGCCC

Product: NAD-dependent epimerase/dehydratase

Products: NA

Alternate protein names: DFR; Dihydrokaempferol 4-reductase [H]

Number of amino acids: Translated: 342; Mature: 342

Protein sequence:

>342_residues
MLQPRARMALAGRRPILVTGGNGFIGQHLVAALRRRHGVVRVLDLQPPPPGPVPEFIQGTILDPHDVRRALDGVDTVYHL
AAISHLWTANPADFERVNQHGTELMLAAARERGVRNIVHCSTEAILFPYRRGGTTMPQRAEDMPGPYTRSKFMAEQIARE
AAADGLRVVIANPTVPIGPGDHNFTEPTRMLDLFARKSPPMVLDSMLNLVDVRDVAAGLILAGERGRAGERYILGGENVS
VRELVRRVGSLCGRSANVHALPASLALAIGAASEWFEGQVIQRTPRVSIEAVRIALRSIPLDTRKAETELGYLPRPIDDA
LTDAIAWLTRRDADAAQPRVSA

Sequences:

>Translated_342_residues
MLQPRARMALAGRRPILVTGGNGFIGQHLVAALRRRHGVVRVLDLQPPPPGPVPEFIQGTILDPHDVRRALDGVDTVYHL
AAISHLWTANPADFERVNQHGTELMLAAARERGVRNIVHCSTEAILFPYRRGGTTMPQRAEDMPGPYTRSKFMAEQIARE
AAADGLRVVIANPTVPIGPGDHNFTEPTRMLDLFARKSPPMVLDSMLNLVDVRDVAAGLILAGERGRAGERYILGGENVS
VRELVRRVGSLCGRSANVHALPASLALAIGAASEWFEGQVIQRTPRVSIEAVRIALRSIPLDTRKAETELGYLPRPIDDA
LTDAIAWLTRRDADAAQPRVSA
>Mature_342_residues
MLQPRARMALAGRRPILVTGGNGFIGQHLVAALRRRHGVVRVLDLQPPPPGPVPEFIQGTILDPHDVRRALDGVDTVYHL
AAISHLWTANPADFERVNQHGTELMLAAARERGVRNIVHCSTEAILFPYRRGGTTMPQRAEDMPGPYTRSKFMAEQIARE
AAADGLRVVIANPTVPIGPGDHNFTEPTRMLDLFARKSPPMVLDSMLNLVDVRDVAAGLILAGERGRAGERYILGGENVS
VRELVRRVGSLCGRSANVHALPASLALAIGAASEWFEGQVIQRTPRVSIEAVRIALRSIPLDTRKAETELGYLPRPIDDA
LTDAIAWLTRRDADAAQPRVSA

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dihydroflavonol-4-reductase family [H]

Homologues:

Organism=Homo sapiens, GI116268111, Length=348, Percent_Identity=27.2988505747126, Blast_Score=84, Evalue=3e-16,
Organism=Homo sapiens, GI193211614, Length=221, Percent_Identity=31.2217194570136, Blast_Score=75, Evalue=9e-14,
Organism=Homo sapiens, GI8393516, Length=221, Percent_Identity=31.2217194570136, Blast_Score=75, Evalue=9e-14,
Organism=Homo sapiens, GI239745448, Length=378, Percent_Identity=26.1904761904762, Blast_Score=75, Evalue=1e-13,
Organism=Homo sapiens, GI310132178, Length=378, Percent_Identity=25.9259259259259, Blast_Score=73, Evalue=4e-13,
Organism=Homo sapiens, GI310113012, Length=378, Percent_Identity=25.9259259259259, Blast_Score=73, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR017829
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =1.1.1.219 [H]

Molecular weight: Translated: 37289; Mature: 37289

Theoretical pI: Translated: 9.84; Mature: 9.84

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLQPRARMALAGRRPILVTGGNGFIGQHLVAALRRRHGVVRVLDLQPPPPGPVPEFIQGT
CCCCHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHCCEEEEEECCCCCCCCCHHHHCCC
ILDPHDVRRALDGVDTVYHLAAISHLWTANPADFERVNQHGTELMLAAARERGVRNIVHC
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCHHHHHHHHHHHCHHHHHHC
STEAILFPYRRGGTTMPQRAEDMPGPYTRSKFMAEQIAREAAADGLRVVIANPTVPIGPG
CCCEEEEEECCCCCCCCCHHHCCCCCHHHHHHHHHHHHHHHHCCCCEEEEECCCEECCCC
DHNFTEPTRMLDLFARKSPPMVLDSMLNLVDVRDVAAGLILAGERGRAGERYILGGENVS
CCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHEEECCCCCCCCEEEECCCCCC
VRELVRRVGSLCGRSANVHALPASLALAIGAASEWFEGQVIQRTPRVSIEAVRIALRSIP
HHHHHHHHHHHHCCCCCCEEHHHHHHHHHHCCHHHHCCCHHHCCCCCHHHHHHHHHHHCC
LDTRKAETELGYLPRPIDDALTDAIAWLTRRDADAAQPRVSA
CCCHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure
MLQPRARMALAGRRPILVTGGNGFIGQHLVAALRRRHGVVRVLDLQPPPPGPVPEFIQGT
CCCCHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHCCEEEEEECCCCCCCCCHHHHCCC
ILDPHDVRRALDGVDTVYHLAAISHLWTANPADFERVNQHGTELMLAAARERGVRNIVHC
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCHHHHHHHHHHHCHHHHHHC
STEAILFPYRRGGTTMPQRAEDMPGPYTRSKFMAEQIAREAAADGLRVVIANPTVPIGPG
CCCEEEEEECCCCCCCCCHHHCCCCCHHHHHHHHHHHHHHHHCCCCEEEEECCCEECCCC
DHNFTEPTRMLDLFARKSPPMVLDSMLNLVDVRDVAAGLILAGERGRAGERYILGGENVS
CCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHEEECCCCCCCCEEEECCCCCC
VRELVRRVGSLCGRSANVHALPASLALAIGAASEWFEGQVIQRTPRVSIEAVRIALRSIP
HHHHHHHHHHHHCCCCCCEEHHHHHHHHHHCCHHHHCCCHHHCCCCCHHHHHHHHHHHCC
LDTRKAETELGYLPRPIDDALTDAIAWLTRRDADAAQPRVSA
CCCHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]