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The map label for this gene is fucA [C]

Identifier: 75675077

GI number: 75675077

Start: 958862

End: 959641

Strand: Direct

Name: fucA [C]

Synonym: Nwi_0882

Alternate gene names: 75675077

Gene position: 958862-959641 (Clockwise)

Preceding gene: 75675075

Following gene: 75675078

Centisome position: 28.18

GC content: 62.05

Gene sequence:

>780_bases
ATGAGCACGATGACTGCGCCTAAAGACGAGCTTGTGAAGCTGGCCGAAAGCCGGATGAAGGTTGCGATCGCGGAGAATAC
CTGGACCGATCGCCAGAAGCTCGCTCTGACCTGCCGCATTCTCTTCGAAGGCGGCCACGATTCCGGTCTGGCCGGCCAGA
TCACGTGCCGCGCTGAGGCGCCAGGAACGTATTACACCCAGCGTCTCGGCCTCGGGTTTGACGAGATCAGGGCGCAGAAC
CTCCTTCTCGTCGATGAGGATCTTAACGTCCTCGATGGCGGCGGCATGGCCAACCCGGCAAACCGGTTTCATAGCTGGCT
CTACCGTGCGCGGCCCGACGTCAACTGCATCATCCATACGCATCCGCTCCATATCGCGGCGCTGTCGACGCTGGAGGTGC
CGCTGGTGGTGTCCCACATGGACCTGTGTCCGCTGTACGGTGACGTCGCGTTCCTCGAAAAGTGGCCCGGGGTGCCCGTC
GGCAACGAGGAAGGCGAAATCATCGCCGAAGCGATCGGTGACAAGAAAGCCATCCTCCTATCCCATCACGGGCAACTGAT
CGCCGGGGCCACGATCGAAGAGGCCTGCGTTCTGGCAATGCTGATCGAACGCGCGGCGAAGATGCAGTTGCTGGCCATGG
CCGCCGGCGAGATCAAGCCGATCCCGGAGAACCTGGCGCGCGAAGCGCATGACTGGATCTCCAAGCCGAAGCGCCACAGC
GCGGCATTCGACTATTACTCCCGCAGAGTTCTGGGTCGCCACAACGACTGCCTGCTCTGA

Upstream 100 bases:

>100_bases
CGCATCCACTTCGAAGACCTGGGAAGCTTCGAGGGGCGTCGAGGAGCGCGGGCTCGTCCAGCAGGCGGCGATACCGGGAT
GAATTTGAAAGGAGTCTACA

Downstream 100 bases:

>100_bases
ACTCCTGCGCTCGACTCCTCCTACTATTTTAAAGGACAGCAAATGCCCGATCTCGCTATTCAAGGCATCATTGGTTACAC
GATCACGCCCTTCGACAACC

Product: class II aldolase/adducin domain protein

Products: Dihydroxyacetone phosphate; L-Lactaldehyde [C]

Alternate protein names: NA

Number of amino acids: Translated: 259; Mature: 258

Protein sequence:

>259_residues
MSTMTAPKDELVKLAESRMKVAIAENTWTDRQKLALTCRILFEGGHDSGLAGQITCRAEAPGTYYTQRLGLGFDEIRAQN
LLLVDEDLNVLDGGGMANPANRFHSWLYRARPDVNCIIHTHPLHIAALSTLEVPLVVSHMDLCPLYGDVAFLEKWPGVPV
GNEEGEIIAEAIGDKKAILLSHHGQLIAGATIEEACVLAMLIERAAKMQLLAMAAGEIKPIPENLAREAHDWISKPKRHS
AAFDYYSRRVLGRHNDCLL

Sequences:

>Translated_259_residues
MSTMTAPKDELVKLAESRMKVAIAENTWTDRQKLALTCRILFEGGHDSGLAGQITCRAEAPGTYYTQRLGLGFDEIRAQN
LLLVDEDLNVLDGGGMANPANRFHSWLYRARPDVNCIIHTHPLHIAALSTLEVPLVVSHMDLCPLYGDVAFLEKWPGVPV
GNEEGEIIAEAIGDKKAILLSHHGQLIAGATIEEACVLAMLIERAAKMQLLAMAAGEIKPIPENLAREAHDWISKPKRHS
AAFDYYSRRVLGRHNDCLL
>Mature_258_residues
STMTAPKDELVKLAESRMKVAIAENTWTDRQKLALTCRILFEGGHDSGLAGQITCRAEAPGTYYTQRLGLGFDEIRAQNL
LLVDEDLNVLDGGGMANPANRFHSWLYRARPDVNCIIHTHPLHIAALSTLEVPLVVSHMDLCPLYGDVAFLEKWPGVPVG
NEEGEIIAEAIGDKKAILLSHHGQLIAGATIEEACVLAMLIERAAKMQLLAMAAGEIKPIPENLAREAHDWISKPKRHSA
AFDYYSRRVLGRHNDCLL

Specific function: Fucose metabolism; third step. [C]

COG id: COG0235

COG function: function code G; Ribulose-5-phosphate 4-epimerase and related epimerases and aldolases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aldolase class II family [H]

Homologues:

Organism=Homo sapiens, GI29826325, Length=203, Percent_Identity=29.5566502463054, Blast_Score=66, Evalue=4e-11,
Organism=Homo sapiens, GI29826323, Length=203, Percent_Identity=29.5566502463054, Blast_Score=65, Evalue=4e-11,
Organism=Homo sapiens, GI9943848, Length=153, Percent_Identity=32.0261437908497, Blast_Score=65, Evalue=6e-11,
Organism=Homo sapiens, GI9951927, Length=153, Percent_Identity=32.0261437908497, Blast_Score=65, Evalue=6e-11,
Organism=Homo sapiens, GI62912453, Length=153, Percent_Identity=32.0261437908497, Blast_Score=65, Evalue=6e-11,
Organism=Homo sapiens, GI29826321, Length=203, Percent_Identity=29.5566502463054, Blast_Score=64, Evalue=1e-10,
Organism=Escherichia coli, GI1789164, Length=197, Percent_Identity=27.4111675126904, Blast_Score=69, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI71989560, Length=200, Percent_Identity=33, Blast_Score=79, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI71989550, Length=200, Percent_Identity=33, Blast_Score=79, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI71989541, Length=200, Percent_Identity=33, Blast_Score=79, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI71989544, Length=200, Percent_Identity=33, Blast_Score=79, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI71989555, Length=200, Percent_Identity=33, Blast_Score=79, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI115533216, Length=194, Percent_Identity=27.8350515463918, Blast_Score=77, Evalue=7e-15,
Organism=Caenorhabditis elegans, GI115533218, Length=199, Percent_Identity=27.1356783919598, Blast_Score=76, Evalue=1e-14,
Organism=Drosophila melanogaster, GI281363755, Length=213, Percent_Identity=30.9859154929577, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI281363751, Length=213, Percent_Identity=30.9859154929577, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24655690, Length=213, Percent_Identity=30.9859154929577, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI281363763, Length=213, Percent_Identity=30.9859154929577, Blast_Score=76, Evalue=2e-14,
Organism=Drosophila melanogaster, GI281363757, Length=213, Percent_Identity=30.9859154929577, Blast_Score=76, Evalue=2e-14,
Organism=Drosophila melanogaster, GI17136738, Length=213, Percent_Identity=30.9859154929577, Blast_Score=76, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24655685, Length=213, Percent_Identity=30.9859154929577, Blast_Score=76, Evalue=3e-14,
Organism=Drosophila melanogaster, GI281363761, Length=213, Percent_Identity=30.9859154929577, Blast_Score=75, Evalue=3e-14,
Organism=Drosophila melanogaster, GI281363759, Length=213, Percent_Identity=30.9859154929577, Blast_Score=75, Evalue=3e-14,
Organism=Drosophila melanogaster, GI281363753, Length=213, Percent_Identity=30.9859154929577, Blast_Score=75, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001303 [H]

Pfam domain/function: PF00596 Aldolase_II [H]

EC number: 4.1.2.17 [C]

Molecular weight: Translated: 28537; Mature: 28406

Theoretical pI: Translated: 6.28; Mature: 6.28

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTMTAPKDELVKLAESRMKVAIAENTWTDRQKLALTCRILFEGGHDSGLAGQITCRAEA
CCCCCCCHHHHHHHHHHHEEEEEECCCCCCCHHHEEEEEEEEECCCCCCCCEEEEEEECC
PGTYYTQRLGLGFDEIRAQNLLLVDEDLNVLDGGGMANPANRFHSWLYRARPDVNCIIHT
CCCHHHHHHCCCHHHHCCCCEEEEECCCCEECCCCCCCHHHHHHHHHHHCCCCCEEEEEE
HPLHIAALSTLEVPLVVSHMDLCPLYGDVAFLEKWPGVPVGNEEGEIIAEAIGDKKAILL
CCCEEEEHHHHCCCHHHHHHHHHHHHCCHHHHHHCCCCCCCCCCCCCHHHHHCCCEEEEE
SHHGQLIAGATIEEACVLAMLIERAAKMQLLAMAAGEIKPIPENLAREAHDWISKPKRHS
ECCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCHHH
AAFDYYSRRVLGRHNDCLL
HHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
STMTAPKDELVKLAESRMKVAIAENTWTDRQKLALTCRILFEGGHDSGLAGQITCRAEA
CCCCCCHHHHHHHHHHHEEEEEECCCCCCCHHHEEEEEEEEECCCCCCCCEEEEEEECC
PGTYYTQRLGLGFDEIRAQNLLLVDEDLNVLDGGGMANPANRFHSWLYRARPDVNCIIHT
CCCHHHHHHCCCHHHHCCCCEEEEECCCCEECCCCCCCHHHHHHHHHHHCCCCCEEEEEE
HPLHIAALSTLEVPLVVSHMDLCPLYGDVAFLEKWPGVPVGNEEGEIIAEAIGDKKAILL
CCCEEEEHHHHCCCHHHHHHHHHHHHCCHHHHHHCCCCCCCCCCCCCHHHHHCCCEEEEE
SHHGQLIAGATIEEACVLAMLIERAAKMQLLAMAAGEIKPIPENLAREAHDWISKPKRHS
ECCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCHHH
AAFDYYSRRVLGRHNDCLL
HHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Ca2+; Co2+; Mg2+; Mn2+; Zn2+ [C]

Kcat value (1/min): 19.3 [C]

Specific activity: 21.0

Km value (mM): 0.7 {L-fuculose} [C]

Substrates: L-Fuculose 1-phosphate [C]

Specific reaction: L-Fuculose 1-phosphate <==> Dihydroxyacetone phosphate + L-Lactaldehyde [C]

General reaction: Elimination of an aldehyde C-C bond; Cleavage [C]

Inhibitor: EDTA; Phosphoglycolohydroxamate [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11259647 [H]